Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.

Abstract:

PURPOSE:The Na+, K+-ATPase (NKA) is important in regulating trans-membrane ion gradients, cellular excitability and muscle function. We investigated the effects of resistance training in healthy young adults on the adaptability of NKA content and of the specific α and β isoforms in human skeletal muscle. METHODS:Twenty-one healthy young males (22.9 ± 4.6 year; 1.80 ± 0.70 m, 85.1 ± 17.8 kg, mean ± SD) underwent 7 weeks of resistance training, training three times per week (RT, n = 16) or control (CON, n = 5). The training program was effective with a 39% gain in leg press muscle strength (p = 0.001). A resting vastus lateralis muscle biopsy was taken before and following RT or CON and assayed for NKA content ([3H]ouabain binding site content) and NKA isoform (α1, α2, β1, β2) abundances. RESULTS:After RT, each of NKA content (12%, 311 ± 76 vs 349 ± 76 pmol g wet weight-1, p = 0.01), NKA α1 (32%, p = 0.01) and α2 (10%, p < 0.01) isoforms were increased, whereas β1 (p = 0.18) and β2 (p = 0.22) isoforms were unchanged. NKA content and isoform abundances were unchanged during CON. CONCLUSIONS:Resistance training increased muscle NKA content through upregulation of both α1 and α2 isoforms, which were independent of β isoform changes. In animal models, modulations in α1 and α2 isoform abundances in skeletal muscle may affect fatigue resistance during exercise, muscle hypertrophy and strength. Whether similar in-vivo functional benefits of these NKA isoform adaptations occurs in human muscle with resistance training remains to be determined.

journal_name

Eur J Appl Physiol

authors

Altarawneh MM,Petersen AC,Farr T,Garnham A,Broatch JR,Halson S,Bishop DJ,McKenna MJ

doi

10.1007/s00421-020-04408-3

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

1777-1785

issue

8

eissn

1439-6319

issn

1439-6327

pii

10.1007/s00421-020-04408-3

journal_volume

120

pub_type

杂志文章
  • Regional differences in facial skin blood flow responses to thermal stimulation.

    abstract:PURPOSE:The facial skin blood flow (SkBF) shows regional differences in the responses to a given stimulation. The facial SkBFs, especially in the eyelid and nose exhibit unique response to physiological and psychological stimuli, but the mechanisms inducing those regional differences remain unclear. To investigate whet...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-019-04109-6

    authors: Miyaji A,Hayashi S,Hayashi N

    更新日期:2019-05-01 00:00:00

  • Respiration-related cerebral blood flow variability increases during control-mode non-invasive ventilation in normovolemia and hypovolemia.

    abstract:PURPOSE:Increased variability in cerebral blood flow (CBF) predisposes to adverse cerebrovascular events. Oscillations in arterial blood pressure and PaCO2 induce CBF variability. Less is known about how heart rate (HR) variability affects CBF. We experimentally reduced respiration-induced HR variability in healthy sub...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-017-3711-0

    authors: Skytioti M,Søvik S,Elstad M

    更新日期:2017-11-01 00:00:00

  • Mobilization and oxidative burst of neutrophils are influenced by carbohydrate supplementation during prolonged cycling in humans.

    abstract::Prolonged, strenuous exercise may lead to suppressive effects on the immune system, which might be responsible for a greater susceptibility to opportunistic infections. The aim of this study was to examine the influence of carbohydrate substitution (CHS) during prolonged, strenuous exercise on neutrophil granulocytes ...

    journal_title:European journal of applied physiology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00421-002-0642-0

    authors: Scharhag J,Meyer T,Gabriel HH,Auracher M,Kindermann W

    更新日期:2002-10-01 00:00:00

  • Inverse relationship between changes of maximal aerobic capacity and changes in walking economy after weight loss.

    abstract:PURPOSE:The aims of this study were to: (1) determine the relationships between maximum oxygen uptake ([Formula: see text]O2max) and walking economy during non-graded and graded walking among overweight women and (2) examine potential differences in [Formula: see text]O2max and walking economy before and after weight l...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-018-3888-x

    authors: Borges JH,Carter SJ,Singh H,Hunter GR

    更新日期:2018-08-01 00:00:00

  • Muscle cooling modulates tissue oxidative and biochemical responses but not energy metabolism during exercise.

    abstract:PURPOSE:This study investigated whether muscle cooling and its associated effects on skeletal muscle oxidative responses, blood gases, and hormonal concentrations influenced energy metabolism during cycling. METHODS:Twelve healthy participants (Males: seven; Females: five) performed two steady-state exercise sessions ...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-020-04407-4

    authors: Gagnon DD,Hancock C,McCue A,Beckett-Brown N,Gagnon J,Williams L,Marsh D,Munten S

    更新日期:2020-08-01 00:00:00

  • Assessment of the efficiency of warming devices during neonatal surgery.

    abstract::This study assessed the relative efficiency of different warming devices (surgical sheets covering the body and a tubegauze on the head, forced-air warming, warming mattress) commonly used to prevent body hypothermia during neonatal surgery. Dry heat losses were measured from a thermal manikin, which simulated a low-b...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-004-1126-1

    authors: Buisson P,Bach V,Elabbassi EB,Chardon K,Delanaud S,Canarelli JP,Libert JP

    更新日期:2004-09-01 00:00:00

  • Effects of muscle group recruitment on sniff transdiaphragmatic pressure and its components.

    abstract::Measuring maximal sniff pressures is an easy way of assessing inspiratory muscle strength. During a static manoeuvre, the pattern of inspiratory muscle recruitment during a sniff can vary from one individual to another. We therefore assessed how voluntarily changing muscle recruitment would affect sniff oesophageal, g...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s004210100469

    authors: Verin E,Delafosse C,Straus C,Morélot-Panzini C,Avdeev S,Derenne JP,Similowski T

    更新日期:2001-10-01 00:00:00

  • Coordination-related changes in the rhythms of breathing and walking in humans.

    abstract::Coordination of the respiratory rhythm with the rhythm of limb movements has often been observed during rhythmical exercise (e.g. in locomotion). It is usually associated with changes in the respiratory time course, but not in the locomotor rhythm. Therefore, we hypothesised that in walking, the extent of coordination...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s004210000224

    authors: Rassler B,Kohl J

    更新日期:2000-07-01 00:00:00

  • Low frequency of the "plateau phenomenon" during maximal exercise in elite British athletes.

    abstract::A plateau in oxygen consumption (VO(2)) has long been considered the criterion for maximal effort during an incremental exercise test. But, surprisingly, the termination of a maximum exercise test often occurs in the absence of a VO(2) plateau. To explain this inconsistency, some have proposed that an oxygen limitatio...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-003-0845-z

    authors: Doherty M,Nobbs L,Noakes TD

    更新日期:2003-08-01 00:00:00

  • The energetics of ultra-endurance running.

    abstract::Our objective was to determine the effects of long-lasting endurance events on the energy cost of running (C(r)), and the role of maximal oxygen uptake (VO(2max)), its fractional utilisation (F) and C(r) in determining the performance. Ten healthy runners (age range 26-59 years) participated in an ultra-endurance comp...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-011-2120-z

    authors: Lazzer S,Salvadego D,Rejc E,Buglione A,Antonutto G,di Prampero PE

    更新日期:2012-05-01 00:00:00

  • Performance for short intermittent runs: active recovery vs. passive recovery.

    abstract::The purpose of this study was to compare the effects of active vs. passive recovery on the time to exhaustion for intermittent runs (15 s) at supramaximal velocity (120% of maximal aerobic speed). Twelve male subjects performed a graded test, an intermittent run to exhaustion with active recovery (50% of maximal aerob...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-003-0834-2

    authors: Dupont G,Blondel N,Berthoin S

    更新日期:2003-08-01 00:00:00

  • Pivoting neuromuscular control and proprioception in females and males.

    abstract:PURPOSE:Noncontact ACL injuries occur most commonly in pivoting sports and are much more frequent in females than in males. However, information on sex differences in proprioceptive acuity under weight-bearing and leg neuromuscular control in pivoting is scarce. The objective of this study was to investigate sex differ...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-014-3062-z

    authors: Lee SJ,Ren Y,Kang SH,Geiger F,Zhang LQ

    更新日期:2015-04-01 00:00:00

  • Effects of plantar stimulation on cardiovascular response to orthostatism.

    abstract:PURPOSE:Walking is a complex locomotor process that involves both spinal cord reflexes and cortical integration of peripheral nerve input. Maintaining an upright body position requires not only neuromuscular activity but also cardiovascular regulation. We postulated that plantar mechanical stimulation might modulate au...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-016-3479-7

    authors: Amirova LE,Navasiolava NM,Bareille MP,Beck A,Tomilovskaya ES,Kozlovzkaya IB,Gauquelin-Koch G,Gharib C,Custaud MA

    更新日期:2016-12-01 00:00:00

  • BP regulation VI: elevated sympathetic outflow with human aging: hypertensive or homeostatic?

    abstract::Though conventional wisdom suggests that a rise in blood pressure is a reality of advancing age, in fact, it appears that progressive elevation in sympathetic activity, not necessarily accompanied by increased blood pressure, is intrinsic to cardiovascular aging in humans. The mechanism behind this elevation would see...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,评审

    doi:10.1007/s00421-013-2731-7

    authors: Taylor JA,Tan CO

    更新日期:2014-03-01 00:00:00

  • Effects of exercise stress on the endocannabinoid system in humans under field conditions.

    abstract::The effects of physical exercise stress on the endocannabinoid system in humans are almost unexplored. In this prospective study, we investigated in a crossover design and under field conditions at different altitudes the effects of physical exercise on the endocannabinoid system (ECS) in 12 trained healthy volunteers...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-011-2237-0

    authors: Feuerecker M,Hauer D,Toth R,Demetz F,Hölzl J,Thiel M,Kaufmann I,Schelling G,Choukèr A

    更新日期:2012-07-01 00:00:00

  • Effects of neuromuscular fatigue on the electromechanical delay of the leg extensors and flexors in young and old men.

    abstract:PURPOSE:The purpose of this study was to investigate the effects of a fatigue-inducing bout of submaximal, intermittent isometric contractions on the electromechanical delay (EMD) of the leg extensors and flexors in young and old men. METHODS:Twenty young (mean ± SD: age = 25 ± 2.8 years) and sixteen old (age = 70.8 ±...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-013-2675-y

    authors: Conchola EC,Thompson BJ,Smith DB

    更新日期:2013-09-01 00:00:00

  • Effects of caffeine ingestion on endurance performance in mentally fatigued individuals.

    abstract:PURPOSE:To examine the effects of caffeine ingestion on physiological and perceptual responses in mentally fatigued individuals. METHODS:Eight male physically active subjects completed four cycling constant-workload tests in four experimental conditions at 80 % of maximal power output: control (C), mental fatigue (MF)...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-016-3483-y

    authors: Azevedo R,Silva-Cavalcante MD,Gualano B,Lima-Silva AE,Bertuzzi R

    更新日期:2016-12-01 00:00:00

  • Ventilatory responses to hypercapnia in divers and non-divers: effects of posture and immersion.

    abstract::The aim of this study was to determine the effects on respiratory drive of two factors, one mechanical (lung volume) and one chemical (sensitivity to hypercapnia), that are involved in determining the breath-hold duration (BHD). Functional residual capacity was measured by helium dilution with the subject seated in ai...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s004210100518

    authors: Delapille P,Verin E,Tourny-Chollet C,Pasquis P

    更新日期:2001-11-01 00:00:00

  • Seasonal variation of haemoglobin mass in internationally competitive female road cyclists.

    abstract::In order to quantify the seasonal variability of haemoglobin mass (Hb(mass)) in cyclists during a competitive season, and investigate whether variability is associated with changes in training load or performance, Hb(mass) was measured in 10 internationally competitive female road cyclists approximately once per month...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-009-1349-2

    authors: Garvican LA,Martin DT,McDonald W,Gore CJ

    更新日期:2010-05-01 00:00:00

  • The use of electromyogram biofeedback to reduce Trendelenburg gait.

    abstract::Ten subjects suffering from incomplete spinal cord injuries, clinically diagnosed as walking with Trendelenburg gait, underwent a 2 month therapy program to strengthen their muscles and reduce their gait abnormalities. Therapy involved muscle strengthening and gait training for 2 h a day, 5 days a week in a clinic. Bi...

    journal_title:European journal of applied physiology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s004210100466

    authors: Petrofsky JS

    更新日期:2001-09-01 00:00:00

  • Rates of fluid ingestion alter pacing but not thermoregulatory responses during prolonged exercise in hot and humid conditions with appropriate convective cooling.

    abstract::The aim of the study was to examine the effects of fluid replacement on thermoregulation and cycling performance in hot, humid conditions. Six male cyclists (PPO = 426 +/- 39 W) performed six 80 km time trials. Subjects replaced 0% (0); 33% (33); 66% (66); or 100% (100) of the weight lost during an "ad libitum" trial ...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-008-0876-6

    authors: Dugas JP,Oosthuizen U,Tucker R,Noakes TD

    更新日期:2009-01-01 00:00:00

  • Post-exercise cooling techniques in hot, humid conditions.

    abstract::Major sporting events are often held in hot and humid environmental conditions. Cooling techniques have been used to reduce the risk of heat illness following exercise. This study compared the efficacy of five cooling techniques, hand immersion (HI), whole body fanning (WBF), an air cooled garment (ACG), a liquid cool...

    journal_title:European journal of applied physiology

    pub_type: 临床试验,杂志文章

    doi:10.1007/s00421-009-1135-1

    authors: Barwood MJ,Davey S,House JR,Tipton MJ

    更新日期:2009-11-01 00:00:00

  • Attenuation of indirect markers of eccentric exercise-induced muscle damage by curcumin.

    abstract:PURPOSE:Polyphenolic curcumin is known to have potent anti-inflammatory effects; thus the present study investigated the hypothesis that curcumin ingestion would attenuate muscle damage after eccentric exercise. METHODS:Fourteen untrained young men (24 ± 1 years) performed 50 maximal isokinetic (120°/s) eccentric cont...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-015-3170-4

    authors: Tanabe Y,Maeda S,Akazawa N,Zempo-Miyaki A,Choi Y,Ra SG,Imaizumi A,Otsuka Y,Nosaka K

    更新日期:2015-09-01 00:00:00

  • Effect of heavy strength training on thigh muscle cross-sectional area, performance determinants, and performance in well-trained cyclists.

    abstract::The purpose of this study was to investigate the effect of heavy strength training on thigh muscle cross-sectional area (CSA), determinants of cycling performance, and cycling performance in well-trained cyclists. Twenty well-trained cyclists were assigned to either usual endurance training combined with heavy strengt...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-009-1307-z

    authors: Rønnestad BR,Hansen EA,Raastad T

    更新日期:2010-03-01 00:00:00

  • Time course of changes in immuneoendocrine markers following an international rugby game.

    abstract::Intense exercise is known to cause temporary impairments in immune function. Few studies, however, have investigated the effects of intense competitive exercise on immunoendocrine variables in elite team sport athletes. The aim of this study was to evaluate the time course of changes in selected immunoendocrine and in...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-009-1200-9

    authors: Cunniffe B,Hore AJ,Whitcombe DM,Jones KP,Baker JS,Davies B

    更新日期:2010-01-01 00:00:00

  • Hold your breath: peripheral and cerebral oxygenation during dry static apnea.

    abstract:PURPOSE:Acute breath-holding deprives the human body from oxygen. In an effort to protect the brain, the diving response is initiated, coupling several physiological responses. The aim of this study was to describe the physiological responses to apnea at the cardiac, peripheral and cerebral level. METHODS:31 physicall...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-020-04445-y

    authors: Bouten J,Bourgois JG,Boone J

    更新日期:2020-10-01 00:00:00

  • High intensity interval training does not impair strength gains in response to resistance training in premenopausal women.

    abstract:PURPOSE:To compare the increases in upper- and lower-body muscle strength in premenopausal women performing resistance training (RT) alone or alongside concurrent high-intensity interval training (CT). METHODS:Sixteen women (26-40 years) were randomly assigned into two groups that performed either RT or CT. Both group...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-017-3614-0

    authors: Gentil P,de Lira CAB,Filho SGC,La Scala Teixeira CV,Steele J,Fisher J,Carneiro JA,Campos MH

    更新日期:2017-06-01 00:00:00

  • Quantitative assessment of nitric oxide in rat skeletal muscle and plasma after exercise.

    abstract::Nitric oxide (NO), a short-lived vasoactive substance that has multiple physiological functions, is also involved in skeletal muscle physiology. This work examines the levels of nitrate (the metabolic end-product of NO) in muscle and plasma after different exercise protocols: namely acute, eccentric, cardiac stress an...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-002-0693-2

    authors: Perez AC,de Oliveira CC,Prieto JG,Ferrando A,Vila L,Alvarez AI

    更新日期:2002-11-01 00:00:00

  • Thermal comfort range of a military cold protection glove: database by thermophysiological simulation.

    abstract::The thermal insulation properties of a military wet/cold protection glove of the German Bundeswehr were investigated using the thermophysiological simulation device CYBOR with a heated full-scale hand model. The aim of this study was the physiology related and more reliable estimation of a database for the thermal com...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章

    doi:10.1007/s00421-007-0660-z

    authors: Zimmermann C,Uedelhoven WH,Kurz B,Glitz KJ

    更新日期:2008-09-01 00:00:00

  • Regenerative responses in slow- and fast-twitch muscles following moderate contusion spinal cord injury and locomotor training.

    abstract::The aim of this study was to use the rat moderate spinal cord contusion model to investigate the effects of incomplete spinal cord injury (SCI) on the muscle regeneration process, comparing regeneration of slow-twitch plantarflexor soleus muscle and fast-twitch dorsiflexor tibialis anterior (TA) muscle. Additionally, ...

    journal_title:European journal of applied physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s00421-012-2429-2

    authors: Jayaraman A,Liu M,Ye F,Walter GA,Vandenborne K

    更新日期:2013-01-01 00:00:00