HSP60 activity on human bronchial epithelial cells.

Abstract:

:HSP60 has been implicated in chronic inflammatory disease pathogenesis, including chronic obstructive pulmonary disease (COPD), but the mechanisms by which this chaperonin would act are poorly understood. A number of studies suggest a role for extracellular HSP60, since it can be secreted from cells and bind Toll-like receptors; however, the effects of this stimulation have never been extensively studied. We investigated the effects (pro- or anti-inflammatory) of HSP60 in human bronchial epithelial cells (16-HBE) alone and in comparison with oxidative, inflammatory, or bacterial challenges. 16-HBE cells were cultured for 1-4 h in the absence or presence of HSP60, H2O2, lipopolysaccharide (LPS), or cytomix. The cell response was evaluated by measuring the expression of IL-8 and IL-10, respectively, pro- and anti-inflammatory cytokines involved in COPD pathogenesis, as well as of pertinent TLR-4 pathway mediators. Stimulation with HSP60 up-regulated IL-8 at mRNA and protein levels and down-regulated IL-10 mRNA and protein. Likewise, CREB1 mRNA was up-regulated. H2O2 and LPS up-regulated IL-8. Experiments with an inhibitor for p38 showed that this mitogen-activated protein kinase could be involved in the HSP60-mediated pro-inflammatory effects. HSP60 showed pro-inflammatory properties in bronchial epithelial cells mediated by activation of TLR-4-related molecules. The results should prompt further studies on more complex ex-vivo or in-vivo models with the aim to elucidate further the role of those molecules in the pathogenesis of COPD.

authors

Sangiorgi C,Vallese D,Gnemmi I,Bucchieri F,Balbi B,Brun P,Leone A,Giordano A,Conway de Macario E,Macario AJ,Cappello F,Di Stefano A

doi

10.1177/0394632017734479

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

333-340

issue

4

eissn

0394-6320

issn

2058-7384

journal_volume

30

pub_type

杂志文章
  • Combined antimuscarinics for treatment of neurogenic overactive bladder.

    abstract::Antimuscarinic drugs are the first line pharmacotherapy for overactive bladder, but they are not always effective to achieve complete continence. Nevertheless in some patients urodynamic investigations reveal insufficient effects with continuing incontinence events even with dose optimization. The aim of this study is...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章,随机对照试验

    doi:10.1177/03946320120250s106

    authors: Nardulli R,Losavio E,Ranieri M,Fiore P,Megna G,Bellomo RG,Cristella G,Megna M

    更新日期:2012-01-01 00:00:00

  • Human mesenchymal stem cells produce bioactive neurotrophic factors: source, individual variability and differentiation issues.

    abstract::The possible use of cell therapies for neurological lesions and disorders is regarded as a very promising strategy. However, many issues related to cell type, tissue donor, expected biological action etc., are still open. In this study human mesenchymal stem cells derived from different fetal and adult tissues were ex...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463201402700309

    authors: Paradisi M,Alviano F,Pirondi S,Lanzoni G,Fernandez M,Lizzo G,Giardino L,Giuliani A,Costa R,Marchionni C,Bonsi L,Calza L

    更新日期:2014-07-01 00:00:00

  • Sublingual allergoid immunotherapy: a new 4-day induction phase in patients allergic to house dust mites.

    abstract::Sublingual immunotherapy with monomeric allergoid (allergoid SLIT), given according to the standard scheme, has proved effective and safe in many clinical trials. However, its build-up phase requires a long time ranging from 16 days to 14 weeks. This study therefore investigated whether, with a four-day up-dosing, the...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章,随机对照试验

    doi:10.1177/039463201002300217

    authors: D'Anneo RW,Bruno ME,Falagiani P

    更新日期:2010-04-01 00:00:00

  • Ultradian variation of nerve growth factor plasma levels in healthy and schizophrenic subjects.

    abstract::Some studies in animal models showed that several neurotrophins may be implicated in the regulation of light-dependent suprachiasmatic pacemaker and in other functions implicated in long-term memory acquisition during sleep. However, no data are known about the role played by NGF in ultradian regulation in humans. The...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 临床试验,杂志文章

    doi:10.1177/039463200401700316

    authors: Bersani G,Iannitelli A,Massoni E,Garavini A,Grilli A,Di Giannantonio M,Conti CM,Pancheri P

    更新日期:2004-09-01 00:00:00

  • Subglotic malt-lymphoma of the larynx: an unusual presentation of chronic cough.

    abstract::Extranodal non-Hodgkin lymphomas limited to the larynx are rare, accounting for less than 1% of all laryngeal neoplasms. The most common site of development of primary laryngeal lymphomas is the supraglottic region. In most cases, the presenting symptoms are hoarseness, dysphagia, dyspnea, and cervical lymphadenopathy...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 信件

    doi:10.1177/039463201402700319

    authors: Zapparoli M,Trolese AR,Remo A,Sina S,Bonetti A,Micheletto C

    更新日期:2014-07-01 00:00:00

  • Acute polyradiculoneuropathy occurring after hymenoptera stings: a clinical case study.

    abstract::Hymenoptera stings may be responsible for both local and systemic reactions; these can be immediate or delayed, depending on the time between the sting and the development of signs or symptoms. Delayed clinical reactions have been reported, although unusual, due to serum sickness and/or affecting organs or systems gen...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463200501800220

    authors: Ridolo E,Albertini R,Borghi L,Meschi T,Montanari E,Dall'Aglio PP

    更新日期:2005-04-01 00:00:00

  • Inhaled mucoactive drugs for treating non-cystic fibrosis bronchiectasis in children.

    abstract::Non-cystic fibrosis bronchiectasis (nCFb) is an acquired condition of variable etiology. Medical treatment basically involves antibiotics and chest physiotherapy. An impaired mucociliary clearance seems to be one of the mechanisms behind nCFb, and inhaled therapy with mucoactive agents has frequently been used to try ...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 信件,评审

    doi:10.1177/039463201302600228

    authors: Snijders D,Calgaro S,Bertozzi I,Quartesan S,Kozuh I,Lunardi F,Barbato A

    更新日期:2013-04-01 00:00:00

  • Infection following bone tumor resection and reconstruction with tumoral prostheses: a literature review.

    abstract::Bone resection is the choice treatment of malignant bone tumors. Tumor prosthesis is one of the most common solutions of reconstruction following resection of bone tumor located to the metaphysis of long bones. Periprosthetic infections are a frequent complication of limb-salvage surgery which is largely due to prolon...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 社论,评审

    doi:10.1177/039463201002300405

    authors: Graci C,Maccauro G,Muratori F,Spinelli MS,Rosa MA,Fabbriciani C

    更新日期:2010-10-01 00:00:00

  • Bone microstructure evaluation near unloaded dental implants combining confocal scanning laser microscopy, circularly polarized light microscopy, and SEM backscattered electrons imaging.

    abstract::The aim of this report is to present a new investigative approach to implant dentistry based on the correlation of qualitative and quantitative data reported on the same figure by overlapping different images collected on the specimen with different investigative systems. Six unloaded titanium dental implants retrieve...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463200702001s09

    authors: Traini T,Degidi M,Murmura G,Piattelli A,Caputi S

    更新日期:2007-01-01 00:00:00

  • Ritonavir and Saquinavir directly stimulate anterior pituitary prolactin secretion, in vitro.

    abstract::An association between human immunodeficiency virus type I (HIV-1) protease inhibitors (PIs) and galactorrhoea/hyperprolactinemia adverse effect has recently been reported in four HIV-1-infected women treated with PIs (indinavir, nelfinavir, ritonavir or saquinavir). This could be explained by a direct effect of riton...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463200201500109

    authors: Orlando G,Brunetti L,Vacca M

    更新日期:2002-01-01 00:00:00

  • Clinical evaluation and treatment of acute asthma exacerbations in children.

    abstract::This update on treatment of asthma exacerbations in children is the result of an Italian Pediatric Society Task-force, made up of a panel of experts working in 2007-2008. The aim is to give clear indications on the use of the drugs most employed in children, grading the quality of evidence and the strength of recommen...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 社论

    doi:10.1177/039463200902200402

    authors: Indinnimeo L,Bertuola F,Cutrera R,De Benedictis FM,Di Pietro P,Duse M,Gianiorio P,Indirli G,La Grutta S,La Rosa M,Longhi R,Miceli Sopo S,Miglioranzi P,Miraglia Del Giudice M,Monaco F,Radzik D,Renna S,Snijders D,Zampog

    更新日期:2009-10-01 00:00:00

  • Response of crypt paneth cells in the small intestine following total-body gamma-irradiation.

    abstract::Ionizing irradiation causes damage and functional failure of irradiation-sensitive systems and tissues such as small intestine. The molecular mechanisms underlying inflammatory and adaptive responses to acute irradiation damage are poorly understood. Using a mouse model of total-body γ-irradiation, we assessed the irr...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463201002300415

    authors: Gorbunov NV,Garrison BR,Kiang JG

    更新日期:2010-10-01 00:00:00

  • Serratiopeptidase reduces the invasion of osteoblasts by Staphylococcus aureus.

    abstract::Finding new strategies to counteract periprosthetic infection and implant failure is a main target in orthopedics. Staphylococcus aureus, the leading etiologic agent of orthopedic implant infections, is able to enter and kill osteoblasts, to stimulate pro-inflammatory chemokine secretion, to recruit osteoclasts, and t...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/0394632017745762

    authors: Selan L,Papa R,Ermocida A,Cellini A,Ettorre E,Vrenna G,Campoccia D,Montanaro L,Arciola CR,Artini M

    更新日期:2017-12-01 00:00:00

  • The POLISMAIL lesson: sublingual immunotherapy may be prescribed also in polysensitized patients.

    abstract::Polysensitization is a feature of allergic rhinitis (AR) that significantly impairs the quality of life (QoL) of AR patients. Allergen-specific immunotherapy is the only causal therapy for AR. However, the polysensitization phenomenon may represent a crucial obstacle as far as it concerns the choice of the allergen ex...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 信件,多中心研究

    doi:10.1177/039463201002300227

    authors: Ciprandi G,Incorvaia C,Puccinelli P,Scurati S,Masieri S,Frati F

    更新日期:2010-04-01 00:00:00

  • Immunomodulatory and antimicrobial efficacy of Lactobacilli against enteropathogenic infection of Salmonella typhi: In-vitro and in-vivo study.

    abstract::Salmonellosis-induced diarrhea, is one of the commonest cause of childhood mortality in developing countries. Using of probiotics is viewed as a promising means for reducing the pathogenic loads of bacterial infection. The current study aimed to evaluate the potential antimicrobial and immunomodulatory efficacy of iso...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/0394632015592099

    authors: Mazaya B,Hamzawy MA,Khalil MA,Tawkol WM,Sabit H

    更新日期:2015-12-01 00:00:00

  • Prognostic implication of high risk human papillomavirus E6 and E7 mRNA in patients with intraepithelial lesions of the cervix in relationship to age.

    abstract::Since the introduction of the cytological screening programs, a significant reduction in the incidence of cervical cancer has been achieved. Almost all of these cancers are related to high-risk (HR) Human Papillomavirus (HPV) cervical infections. However, the natural history of HPV infection seems to be different in y...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463201102400219

    authors: Frega A,Lorenzon L,Giovagnoli MR,De Sanctis L,Fabiano V,Lukic A,Moscarini M,Torrisi MR,French D

    更新日期:2011-04-01 00:00:00

  • Systemic nickel allergy: oral desensitization and possible role of cytokines interleukins 2 and 10.

    abstract::Nickel ingested with food can elicit either systemic cutaneous or gastrointestinal symptoms causing a systemic nickel allergy syndrome (SNAS) that can be treated with tolerance by oral ingestion of the metal. It has been suggested that interleukins 2 (IL-2) and 10 (IL-10) are involved in the mechanisms underlying oral...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 临床试验,信件

    doi:10.1177/039463201302600127

    authors: Ricciardi L,Carni A,Loschiavo G,Gangemi S,Tigano V,Arena E,Mannucci C,Calapai G

    更新日期:2013-01-01 00:00:00

  • A multicenter open-label experience on the response of psoriasis to Adalimumab and effect of dose escalation in non-responders: the Aphrodite project.

    abstract::There is much evidence to show the efficacy of adalimumab, a human monoclonal antibody targeting tumour necrosis factor-alpha, in the treatment of plaque psoriasis. In this open-label experience, 147 high-need patients suffering from plaque psoriasis, with a mean Psoriasis Area and Severity Index (PASI) of 18.8, and c...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章,多中心研究

    doi:10.1177/039463200902200125

    authors: Vena GA,Galluccio A,De Simone C,Mastrandrea V,Buquicchio R,La Greca S,Dattola S,Puglisi Guerra A,Donato L,Cantoresi F,De Pità O,Pezza M,D Agostino M,Vernaci R,Miracapillo A,Valenti G,Cassano N

    更新日期:2009-01-01 00:00:00

  • Detection of hepatitis C virus-RNA in saliva from chronically HCV-infected patients.

    abstract::The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids, including saliva. From a review of the literature many investigators have found the presence of HCV-RNA in saliva, however, widely contrasting results em...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:

    authors: Pastore L,Fiore JR,Tateo M,De Benedittis M,Petruzzi M,Casalino C,Genchi C,Lo Muzio L,Angarano G,Serpico R

    更新日期:2006-01-01 00:00:00

  • A case of Smith-Lemli-Opitz Syndrome, defect of cholesterol biosynthesis.

    abstract::We report the case of a child with Smith-Lemli-Opitz Syndrome. The pregnancy was complicated by prenatal growth retardation. The baby was admitted to the Neonatal Intensive Care Unit of Chieti when she was five months old. She showed postnatal growth retardation, trouble sucking and swallowing, microcephaly and multip...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463200601900220

    authors: Domizio S,Pallotta R,Romanelli A,Puglielli C,Pollice R,Casacchia M,Conte E,Domizio R,Sabatino G

    更新日期:2006-04-01 00:00:00

  • Specific immunotherapy in children: the evidence.

    abstract::Specific immunotherapy (SIT) is the only treatment able to not only act on the symptoms of allergy but also act on the causes. At present, SIT may be administered in two forms: subcutaneous (SCIT) and sublingual immunotherapy (SLIT). SCIT represents the standard modality of treatment while SLIT has recently been intro...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1177/03946320110240S413

    authors: La Rosa M,Lionetti E,Leonardi S,Salpietro A,Bianchi L,Salpietro C,Miraglia Del Giudice M,Ciprandi G,Marseglia GL

    更新日期:2011-10-01 00:00:00

  • Aberrant miR-219-5p is correlated with TLR4 and serves as a novel biomarker in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning.

    abstract::This study aimed to investigate the clinical significance of serum microRNA-219-5p (miR-219-5p) in patients with multiple organ dysfunction syndrome (MODS) caused by acute paraquat (PQ) poisoning, and its correlation with Toll-like Receptor 4 (TLR4). Luciferase reporter assay was used to investigate in vitro the corre...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/2058738420974888

    authors: Dai Y,Liu X,Gao Y

    更新日期:2020-01-01 00:00:00

  • Safety and efficacy of etanercept in children with juvenile-onset Behcets disease.

    abstract::Behcets disease (BD) is a chronic, relapsing, multi-system inflammatory disorder, clinically characterized by recurrent oral and genital ulcers, skin lesions, and uveitis. Other manifestations include arthritis, a positive pathergy test, thrombophlebitis, central nervous system disease and gastrointestinal ulcerations...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 信件

    doi:10.1177/039463200902200235

    authors: Cantarini L,Tinazzi I,Caramaschi P,Bellisai F,Brogna A,Galeazzi M

    更新日期:2009-04-01 00:00:00

  • Aurora B expression as a prognostic indicator and possible therapeutic target in oral squamous cell carcinoma.

    abstract::The aim of this study is to investigate the expression of the chromosomal passenger protein Aurora B and its activated (phosphorylated) form in a large series of human oral squamous cell cancers (OSCC) and to evaluate its clinical and prognostic significance. Western blotting analysis revealed overexpression of both A...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463201102400110

    authors: Pannone G,Hindi SA,Santoro A,Sanguedolce F,Rubini C,Cincione RI,De Maria S,Tortorella S,Rocchetti R,Cagiano S,Pedicillo C,Serpico R,Lo Muzio L,Bufo P

    更新日期:2011-01-01 00:00:00

  • Interleukin-2 enhances susceptibility of colon cancer cells to FasR mediated apoptosis by up-regulating Fas receptor level and down-regulating FAP-1 expression.

    abstract::Colon cancer cells are resistant to FasR-mediated apoptosis, which contributes to their evasion from immune attack by CTLs and NK cells. FasR refractoriness of the malignancies was reported to result from loss of Fas receptors and overepression of Fas-associated phosphatase-1 (FAP-1). Therefore, we investigated the ef...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:

    authors: Song E,Chen J,Antus B,Wang M,Xie Y,Yao H,Exton MS

    更新日期:2000-09-01 00:00:00

  • Determinants of bronchial hyperresponsiveness in subjects with rhinitis.

    abstract::Subjects with rhinitis but without asthma may have coexisting bronchial hyperresponsiveness, although the reasons for this are uncertain. To evaluate the factors that determine BHR in rhinitis we examined 410 patients with symptomatic rhinitis with forced expiratory volume in 1 second (FEV1) and forced vital capacity ...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463200501800414

    authors: Di Lorenzo G,Pacor ML,Mansueto P,Esposito Pellitteri M,Lo Bianco C,Ditta V,Leto-Barone MS,Napoli N,Di Fede G,Rini GB

    更新日期:2005-10-01 00:00:00

  • Increased levels of NF-kappaB inhibitors (IkappaBalpha and IkappaBgamma) in the intestinal mucosa of Crohn's disease patients during infliximab treatment.

    abstract::The treatment with infliximab is employed successfully in Crohn's disease (CD) but predictors of efficacy are lacking. Activation of the transcription factor NF-kB has been demonstrated in CD and its inhibition is one of the mechanisms by which anti-inflammatory agents exert their effects. We evaluated the production ...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463200501800116

    authors: Guidi L,Costanzo M,Ciarniello M,De Vitis I,Pioli C,Gatta L,Pace L,Tricerri A,Bartoloni C,Coppola L,Balistreri P,Doria G,Fedeli G,Gasbarrini GB

    更新日期:2005-01-01 00:00:00

  • Pathology of upper tract urothelial carcinoma with emphasis on staging.

    abstract::Classification of upper tract urothelial preneoplastic and neoplastic lesions mirrors that of the urinary bladder, with all lesions of the bladder urothelium being possible in the upper tract and vice versa. There are three major groups of non-invasive urothelial neoplasms: flat, papillary, and inverted. These three g...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 社论,评审

    doi:10.1177/039463201402700406

    authors: Mazzucchelli R,Scarpelli M,Galosi AB,Di Primio R,Lopez-Beltran A,Cheng L,Montironi R

    更新日期:2014-10-01 00:00:00

  • CD1A and CD1E gene polymorphisms are associated with susceptibility to multiple sclerosis.

    abstract::Multiple sclerosis (MS) is thought to be an autoimmune T-cell-mediated disease directed at myelin antigens of the central nervous system. Besides myelin proteins, lipid components of CNS are supposed to play a role as antigens for T cells in MS. CD1 is a family of MHC-like glycoproteins specialized in capturing and pr...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463201102400120

    authors: Caporale CM,Notturno F,Pace M,Aureli A,Di Tommaso V,De Luca G,Farina D,Giovannini A,Uncini A

    更新日期:2011-01-01 00:00:00

  • IL-8 and pH values in exhaled condensate after antibiotics in cystic fibrosis children.

    abstract::Interleukin (IL)-8 is a major factor in inflammatory response and the IL-8 levels in exhaled breath condensate (EBC) may be used as a marker of airway inflammation. Airway acidification is implicated in the pathophysiology of obstructive airway diseases and pH EBC values have been used as a marker of airway acidificat...

    journal_title:International journal of immunopathology and pharmacology

    pub_type: 杂志文章

    doi:10.1177/039463200702000305

    authors: Bodini A,D'Orazio C,Peroni DG,Corradi M,Zerman L,Folesani G,Assael BM,Boner AL,Piacentini GL

    更新日期:2007-07-01 00:00:00