Abstract：
:In this paper, a new strategy is reported for preparing a label-free β-trophin electrochemiluminescent (ECL) immunosensor with good specificity, reproducibility and stability. An aquagel polymer from the hydrolysis of (3-aminopropyl) trimethoxysilane acted as the linker to catch the Au nanoparticles (AuNPs) on the indium-tin oxide (ITO) substrate by a two-step method. The AuNPs play an important role in enhancing ECL and immobilizing the β-trophin antibody. This immunosensor can test for β-trophin using luminol as an ECL probe. The ECL intensity at the resultant sensor, after the direct immuno-interaction, was proportional to the concentration of β-trophin and had a low limit of quantification as 4.2 ng mL-1. After deep discussions on the ECL mechanism of this immunosensor, we found that its sensitivity is greatly affected by the presence of oxygen and improved under deoxygenation. We believe that this sensor can be used for clinical cases.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zheng L,Fang C,Yan J,Li H,Tu Ydoi
10.1038/s41598-017-11750-8subject
Has Abstractpub_date
2017-09-11 00:00:00pages
11199issue
1issn
2045-2322pii
10.1038/s41598-017-11750-8journal_volume
7pub_type
杂志文章abstract：:The incidence of ovarian cancer dramatically increases in early menopause but the factors contributing to cancer onset are unclear. Most ovarian cancers originate in the fallopian tube with subsequent implantation of malignant cells into the ovary. However, the events and conditions that lead to cancer cell implantati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30261-8
更新日期：2018-08-17 00:00:00
abstract：:The mosquito, Aedes baisasi, which inhabits brackish mangrove swamps, is known to feed on fish. However, its host assemblage has not been investigated at the species level. We amplified and sequenced the cytochrome oxidase subunit I barcoding regions as well as some other regions from blood-fed females to identify hos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40509-6
更新日期：2019-03-08 00:00:00
abstract：:Present study focuses on rainfall over Western Ghats (WG), a complex topographic region (elevation > 500 m) of India to evaluate and to better understand the satellite behavior in contrast with a flat region (FR) (elevation < 500 m) of central India from 1998 to 2016 using the combinatory data sets of TMPA and IMERG (...
journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-07-11 00:00:00
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journal_title：Scientific reports
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更新日期：2018-05-02 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2017-09-28 00:00:00
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pub_type： 杂志文章
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更新日期：2017-11-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-11-04 00:00:00
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更新日期：2016-05-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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pub_type： 杂志文章,meta分析,评审
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更新日期：2020-01-29 00:00:00
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更新日期：2016-07-04 00:00:00
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pub_type： 杂志文章
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