Abstract：
:Tyrosylprotein sulfotransferases (TPSTs) are enzymes that catalyze post-translational tyrosine sulfation of proteins. In humans, there are only two TPST isoforms, designated TPST1 and TPST2. In a previous study, we reported the crystal structure of TPST2, which revealed the catalytic mechanism of the tyrosine sulfation reaction. However, detailed molecular mechanisms underlying how TPSTs catalyse a variety of substrate proteins with different efficiencies and how TPSTs catalyze the sulfation of multiple tyrosine residues in a substrate protein remain unresolved. Here, we report two crystal structures of the human TPST1 complexed with two substrate peptides that are catalysed by human TPST1 with significantly different efficiencies. The distinct binding modes found in the two complexes provide insight into the sulfation mechanism for these substrates. The present study provides valuable information describing the molecular mechanism of post-translational protein modifications catalysed by TPSTs.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Tanaka S,Nishiyori T,Kojo H,Otsubo R,Tsuruta M,Kurogi K,Liu MC,Suiko M,Sakakibara Y,Kakuta Ydoi
10.1038/s41598-017-07141-8subject
Has Abstractpub_date
2017-08-18 00:00:00pages
8776issue
1issn
2045-2322pii
10.1038/s41598-017-07141-8journal_volume
7pub_type
杂志文章abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-75487-7
更新日期：2020-10-22 00:00:00
abstract：:The production of tuberous roots is usually reduced by vigorous vegetative growth because of the competition for resource between the vegetative parts and reproductive organs. In this study, we conducted root pruning to examine the vigorous vegetative growth by regulating root growth, subsequently limiting vegetative ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22586-1
更新日期：2018-03-13 00:00:00
abstract：:The clotting factor prothrombin exists in equilibrium between closed and open conformations, but the physiological role of these forms remains unclear. As for other allosteric proteins, elucidation of the linkage between molecular transitions and function is facilitated by reagents stabilized in each of the alternativ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21304-1
更新日期：2018-02-13 00:00:00
abstract：:The full length human histone 3 lysine 4 demethylase KDM5B (PLU-1/Jarid1B) has been studied using Hydrogen/Deuterium exchange mass spectrometry, homology modelling, sequence analysis, small angle X-ray scattering and electron microscopy. This first structure on an intact multi-domain Jumonji histone demethylase reveal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40573-y
更新日期：2019-03-11 00:00:00
abstract：:In Duchenne muscular dystrophy, progressive loss of muscle tissue is accompanied by fibrosis, chronic inflammation and reduced muscle regenerative capacity. Although much is known about the development of fibrosis and chronic inflammation in muscular dystrophy, less is known about how they are mechanistically linked t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24708
更新日期：2016-05-31 00:00:00
abstract：:Obese individuals can be categorized as "healthy obese" (MHO) and "unhealthy obese" (MUO) based on the presence or absence of metabolic abnormality. This study sets out to assess potential genetic causes behind persistence of healthy metabolic status in individuals categorized as "healthy obese". This study was conduc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70627-5
更新日期：2020-08-12 00:00:00
abstract：:The aim of this study was to investigate whether a novel physiologically relevant marker, InsuTAG (fasting insulin × fasting triglycerides) can predict insulin resistance (IR) and metabolic syndrome (MetS). Data of 618 participants from the Retirement Health and Lifestyle Study (RHLS) were evaluated for the current st...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15460-z
更新日期：2017-11-09 00:00:00
abstract：:While most large river-deltas in the world are facing the risk of subsidence and erosion in the Anthropocene, it is suspected that the Changjiang submerged delta (CSD) could be subjected to the impacts of the world's largest dam, the Three Gorges Dam (TGD). Here we firstly indicate that the CSD went through high accum...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06600
更新日期：2014-10-16 00:00:00
abstract：:Whether the retinal process alone or retinal and cortical processes jointly determine afterimage (AI) formation has long been debated. Based on the retinal rebound responses, recent work proposes that afterimage signals are exclusively generated in the retina, although later modified by cortical mechanisms. We tested ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41101
更新日期：2017-01-23 00:00:00
abstract：:Aberrant immune responses characterize autoimmune disorders like Rheumatoid Arthritis (RA) wherein lymphocytes are recognized as key players. Role of CD8+ T cells in RA has been less defined however we found that these cells are activated in RA patients with increased expression of cytolytic granules and inflammatory ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01033-7
更新日期：2017-04-19 00:00:00
abstract：:In the era of precision medicine the treatment options for cancer patients and subsequent outcomes are expected to improve. We present a review of patients enrolled in first-in-human Phase1 trials at University of Alabama at Birmingham. Between 1/2015-6/2017, 162 cancer patients (whole cohort, WC) were enrolled on pha...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
doi：10.1038/s41598-020-64906-4
更新日期：2020-05-13 00:00:00
abstract：:The epithelial-to-mesenchymal transition (EMT) is one of mechanisms that induce renal interstitial fibrosis. Understanding EMT in renal fibrosis has important therapeutic implications for patients with kidney disease. Alpha-lipoic acid (ALA) is a natural compound with antioxidant properties. Studies for ALA are perfor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46065
更新日期：2017-04-05 00:00:00
abstract：:A patient's pain during mandibular third molar extraction often creates problems for a dental surgeon and can also cause immense patient discomfort, such as decreased quality of life, serious complications, or even danger to the patients' lives. Effective pain management is therefore of great importance. Conventional ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
doi：10.1038/srep40987
更新日期：2017-01-20 00:00:00
abstract：:TRIB1 is a GWAS locus associated with plasma cholesterol and triglycerides (TG) levels. In mice, liver-specific overexpression of TRIB1 lowers plasma lipid levels. Berberine (BBR) is a natural lipid lowering drug that reduces plasma LDL-cholesterol (LDL-C), total cholesterol (TC) and TG in hyperlipidemic patients and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52253-y
更新日期：2019-10-30 00:00:00
abstract：:Chloroplast genomes have been widely considered an informative and valuable resource for molecular marker development and phylogenetic reconstruction in plant species. This study evaluated the complete chloroplast genomes of the traditional Chinese medicine Gleditsia sinensis and G. japonica, an adulterant of the form...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73392-7
更新日期：2020-10-01 00:00:00
abstract：:The Rho-family small GTPase Cdc42 localizes at plasma membrane and Golgi complex and aside from protrusion and migration operates in vesicle trafficking, endo- and exocytosis as well as establishment and/or maintenance of cell polarity. The formin family members FMNL2 and -3 are actin assembly factors established to r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09952-1
更新日期：2017-08-29 00:00:00
abstract：:Although N-acyl-L-homoserine lactone (AHL) based quorum sensing (QS) phenomenon has been observed in mature anaerobic granules, the biological role of AHL-based QS system in anaerobic granulation process remains unexplored. For the first time, a long-term anaerobic bioreactor was operated for 168 days to investigate t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34183-3
更新日期：2018-10-25 00:00:00
abstract：:The second-in-class proteasome inhibitor (PI) carfilzomib (Kyprolis, Cfz) has contributed to a substantial advancement in multiple myeloma treatment by improving patient survival and quality of life. A considerable portion of patients however display intrinsic resistance to Cfz. Our mechanistic understanding of intrin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40635-1
更新日期：2019-03-11 00:00:00
abstract：:In this study, nanowires/nanowalls were generated on a silicon wafer through a chemical etching method. Octadecyltrichlorosilane (OTS) was deposited onto the nanowire/nanowall surfaces to alter their hydrophobicity. The hydrophobic characteristics of the surfaces were further modified via a 1.5-μm-thick layer of n-oct...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38678
更新日期：2016-12-09 00:00:00
abstract：:2D materials open the possibility to study Dirac electrons in complex self-similar geometries. The two-dimensional nature of materials like graphene, silicene, phosphorene and transition-metal dichalcogenides allow the nanostructuration of complex geometries through metallic electrodes, interacting substrates, strain,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71697-1
更新日期：2020-09-07 00:00:00
abstract：:Nuclei detection is often a critical initial step in the development of computer aided diagnosis and prognosis schemes in the context of digital pathology images. While over the last few years, a number of nuclei detection methods have been proposed, most of these approaches make idealistic assumptions about the stain...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33985
更新日期：2016-10-03 00:00:00
abstract：:Dravet syndrome is the prototype of SCN1A-mutation associated epilepsies. It is characterised by prolonged seizures, typically provoked by fever. We describe the evaluation of an SCN1A mutation in a child with early-onset temperature-sensitive seizures. The patient carries a heterozygous missense variant (c3818C > T; ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31879
更新日期：2016-09-01 00:00:00
abstract：:Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic swit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14455
更新日期：2015-09-24 00:00:00
abstract：:CRISPR-Cas9/gRNA exhibits therapeutic efficacy against latent human immunodeficiency virus (HIV) genome but the delivery of this therapeutic cargo to the brain remains as a challenge. In this research, for the first time, we demonstrated magnetically guided non-invasive delivery of a nano-formulation (NF), composed of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40222-4
更新日期：2019-03-08 00:00:00
abstract：:A growing interest in healthy eating has lead to an increase in the consumption of vegetables, associated with a rising number of bacterial outbreaks related to fresh produce. This is the case of the outbreak in Germany, caused by a O104:H4 enteroaggregative E. coli strain lysogenic for a Stx phage. Temperate Stx phag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08169-6
更新日期：2017-08-10 00:00:00
abstract：:Although the effects of osteoporosis on the skeleton are well studied, site-specific and long-term studies on the mandible are still lacking. This study investigated the time-course changes of the bone microarchitecture in the mandibular condyle in comparison to the corresponding changes in the alveolar bone, body of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50554-w
更新日期：2019-10-02 00:00:00
abstract：:The hyaluronan synthase 2 (HAS2)-hyaluronic acid (HA)-CD44-Caspase-3 pathway is involved in ovarian granulosa cell (GC) functions in mammals. HAS2 is a key enzyme required for HA synthesis and is the key factor in this pathway. However, the regulation of HAS2 and the HAS2-mediated pathway by microRNAs in GCs is poorly...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21197
更新日期：2016-02-18 00:00:00
abstract：:Wolfram syndrome (WS) is a rare neurodegenerative disorder that is mainly characterized by diabetes mellitus, optic nerve atrophy, deafness, and progressive brainstem degeneration. Treatment with GLP-1 receptor agonists has shown a promising anti-diabetic effect in WS treatment in both animal models and in human patie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52295-2
更新日期：2019-10-31 00:00:00
abstract：:Epidemiologic studies have identified a positive association between obesity and colorectal neoplasia. Adiposity induces systemic low-grade inflammation, which is commonly assessed with a sensitive biomarker, C-reactive protein (CRP). To understand the molecular mechanisms of obesity in the etiology of colorectal neop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04780-9
更新日期：2017-06-30 00:00:00
abstract：:SOCS2 is a pleiotropic E3 ligase. Its deficiency is associated with gigantism and organismal lethality upon inflammatory challenge. However, mechanistic understanding of SOCS2 function is dismal due to our unawareness of its protein substrates. We performed a mass spectrometry based proteomic profiling upon SOCS2 depl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42800
更新日期：2017-02-20 00:00:00
