Optimization of neuronal cultures from rat superior cervical ganglia for dual patch recording.

Abstract:

:Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic switch and to develop mature and healthy neuronal profiles suited for detailed patch clamp analysis. One single pup provides sufficient material to prepare what was formerly obtained from 12 to 15 animals. The suitability of these cultures to study neurotransmitter release mechanisms was validated by presynaptically perturbing the interaction of the v-SNARE VAMP2 with the vesicular V-ATPase V0c subunit.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Amendola J,Boumedine N,Sangiardi M,El Far O

doi

10.1038/srep14455

subject

Has Abstract

pub_date

2015-09-24 00:00:00

pages

14455

issn

2045-2322

pii

srep14455

journal_volume

5

pub_type

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