Abstract:
:Superior cervical ganglion neurons (SCGN) are often used to investigate neurotransmitter release mechanisms. In this study, we optimized the dissociation and culture conditions of rat SCGN cultures for dual patch clamp recordings. Two weeks in vitro are sufficient to achieve a significant CNTF-induced cholinergic switch and to develop mature and healthy neuronal profiles suited for detailed patch clamp analysis. One single pup provides sufficient material to prepare what was formerly obtained from 12 to 15 animals. The suitability of these cultures to study neurotransmitter release mechanisms was validated by presynaptically perturbing the interaction of the v-SNARE VAMP2 with the vesicular V-ATPase V0c subunit.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Amendola J,Boumedine N,Sangiardi M,El Far Odoi
10.1038/srep14455subject
Has Abstractpub_date
2015-09-24 00:00:00pages
14455issn
2045-2322pii
srep14455journal_volume
5pub_type
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