Abstract：
:CRISPR-Cas9/gRNA exhibits therapeutic efficacy against latent human immunodeficiency virus (HIV) genome but the delivery of this therapeutic cargo to the brain remains as a challenge. In this research, for the first time, we demonstrated magnetically guided non-invasive delivery of a nano-formulation (NF), composed of Cas9/gRNA bound with magneto-electric nanoparticles (MENPs), across the blood-brain barrier (BBB) to inhibit latent HIV-1 infection in microglial (hμglia)/HIV (HC69) cells. An optimized ac-magnetic field of 60 Oe was applied on NF to release Cas9/gRNA from MENPs surface and to facilitate NF cell uptake resulting in intracellular release and inhibition of HIV. The outcomes suggested that developed NF reduced HIV-LTR expression significantly in comparison to unbound Cas9/gRNA in HIV latent hμglia/HIV (HC69) cells. These findings were also validated qualitatively using fluorescence microscopy to assess NF efficacy against latent HIV in the microglia cells. We believe that CNS delivery of NF (CRISPR/Cas9-gRNA-MENPs) across the BBB certainly will have clinical utility as future personalized nanomedicine to manage neuroHIV/AIDS.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kaushik A,Yndart A,Atluri V,Tiwari S,Tomitaka A,Gupta P,Jayant RD,Alvarez-Carbonell D,Khalili K,Nair Mdoi
10.1038/s41598-019-40222-4subject
Has Abstractpub_date
2019-03-08 00:00:00pages
3928issue
1issn
2045-2322pii
10.1038/s41598-019-40222-4journal_volume
9pub_type
杂志文章abstract：:Nonequilibrium can be a source of order. This rather counterintuitive statement has been proven to be true through a variety of fluctuation-driven, self-organization behaviors exhibited by out-of-equilibrium, many-body systems in nature (physical, chemical, and biological), resulting in the spontaneous appearance of m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03283
更新日期：2013-11-21 00:00:00
abstract：:Blood transfusion is fundamental in managing hematologic malignancies. We sought to evaluate the need and availability of blood products for patients with hematological malignancies at Uganda Cancer Institute. We prospectively studied the demand and supply of blood for patients with thrombocytopenia (platelet count ≤5...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59773-y
更新日期：2020-02-18 00:00:00
abstract：:Hox proteins are transcription factors and key regulators of segmental identity along the anterior posterior axis across all bilaterian animals. Despite decades of research, the mechanisms by which Hox proteins select and regulate their targets remain elusive. We have carried out whole-genome ChIP-chip experiments to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00205
更新日期：2011-01-01 00:00:00
abstract：:Since the re-emergence of Zika virus in 2014 and subsequent association with microcephaly, much work has focused on the development of a vaccine to halt its spread throughout the world. The mosquito vector that transmits this virus is widespread and responsible for the spread of other arboviridae including Dengue. Cur...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05980-z
更新日期：2017-07-24 00:00:00
abstract：:Many deltas are likely undergoing net erosion because of rapid decreases in riverine sediment supply and rising global sea levels. However, detecting erosion in subaqueous deltas is usually difficult because of the lack of bathymetric data. In this study, by comparing bathymetric data between 1981 and 2012 and surfici...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41735
更新日期：2017-02-01 00:00:00
abstract：:Banana production is severely hindered by plant-parasitic nematodes in acidic, sandy soil. This study investigated the possibility of applying a novel fumigation agent based on ammonium bicarbonate as a strategy for controlling plant-parasitic nematodes under sealed conditions. Moreover, its effects on the nematode co...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17597
更新日期：2015-12-01 00:00:00
abstract：:N-linked glycosylation is one of the predominant post-translational modifications involved in a number of biological functions. Since experimental characterization of glycosites is challenging, glycosite prediction is crucial. Several predictors have been made available and report high performance. Most of them evalua...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52341-z
更新日期：2019-11-04 00:00:00
abstract：:We present a density functional theory (DFT) for steady-state nonequilibrium quantum systems such as molecular junctions under a finite bias. Based on the steady-state nonequilibrium statistics that maps nonequilibrium to an effective equilibrium, we show that ground-state DFT (GS-DFT) is not applicable in this case a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15386
更新日期：2015-10-16 00:00:00
abstract：:This study aimed to explore the effects of radiochemotherapy on the neurocognitive function of patients with high-grade gliomas (HGG). The mini-mental state examination (MMSE), Montreal Cognitive Assessment (MoCA), event-related potential P300 (ERP-P300), and specific MRI parameters were compared, and the associations...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42321-8
更新日期：2019-04-12 00:00:00
abstract：:Psychopathy is a personality disorder characterised by atypical moral behaviour likely rooted in atypical affective/motivational processing, as opposed to an inability to judge the wrongness of an action. Guilt is a moral emotion believed to play a crucial role in adherence to moral and social norms, but the mechanism...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36273
更新日期：2016-11-03 00:00:00
abstract：:Here we show that electrical tuning of the sensitivity of chemitransistor sensors, namely field-effect-transistors (FETs) exploiting nano/mesostructured sensing materials, can be used to effectively address two chief problems of state-of-the-art gas sensors, specifically fabrication reliability and degradation by agin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01161
更新日期：2013-01-01 00:00:00
abstract：:The accident at the Fukushima Daiichi nuclear power plant caused serious radiocesium ((137)Cs) contamination of forest ecosystems located in mountainous and hilly regions with steep terrain. To understand topographic effects on the redistribution and accumulation of (137)Cs on forest floor, we investigated the distrib...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06853
更新日期：2014-10-31 00:00:00
abstract：:The aim of our study was to compare soft tissue measurements with 3D imaging methods in individuals with untreated skeletal and pseudo-Class III malocclusions. The study sample consisted of 75 patients (38 males, 37 females, mean age 12.41 ± 2.35 years) with pseudo- and true skeletal Class III malocclusions and skelet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71772-7
更新日期：2020-09-07 00:00:00
abstract：:A method is described for multi-residue, high-throughput determination of trace levels of 22 organochlorine pesticides (OCPs) and 5 pyrethroid pesticides (PYPs) in Chinese medicinal (CM) health wines using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) based extraction method and gas chromatography-elect...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21558
更新日期：2016-02-17 00:00:00
abstract：:Airports can affect birds by hindering acoustic communication. Here, we investigated the impacts of aircraft events on vocal behavior in wood thrush (Hylocichla mustelina) breeding one mile from an airport in Ithaca, NY, USA. We identified the number of wood thrush songs between 0500 and 0800 h at various distances fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80380-4
更新日期：2021-01-13 00:00:00
abstract：:The human cytomegalovirus developed distinct evasion mechanisms from the cellular antiviral response involving vMIA, a virally-encoded protein that is not only able to prevent cellular apoptosis but also to inhibit signalling downstream from mitochondrial MAVS. vMIA has been shown to localize at mitochondria and to tr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26028
更新日期：2016-05-16 00:00:00
abstract：:Significant technical and optical advances are required for intraoperative optical coherence tomography (OCT) to be utilized during otological surgeries. Integrating OCT with surgical microscopy makes it possible to evaluate soft tissue in real-time and at a high resolution. Herein, we describe an augmented-reality, i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35563-5
更新日期：2018-11-27 00:00:00
abstract：:The salamander chytrid fungus (Batrachochytrium salamandrivorans [Bsal]) is causing massive mortality of salamanders in Europe. The potential for spread via international trade into North America and the high diversity of salamanders has catalyzed concern about Bsal in the U.S. Surveillance programs for invading patho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69486-x
更新日期：2020-08-03 00:00:00
abstract：:Fat character is an important index in duck culture that linked to local flavor, feed cost and fat intake for costumers. Since the regulation networks in duck lipid metabolism had not been reported very clearly, we aimed to explore the potential miRNA-mRNA pairs and their regulatory roles in duck lipid metabolism. Her...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27418
更新日期：2016-06-08 00:00:00
abstract：:Phosphopantetheinyl transferases (PPTases) play essential roles in both primary metabolisms and secondary metabolisms via post-translational modification of acyl carrier proteins (ACPs) and peptidyl carrier proteins (PCPs). In this study, an industrial FK506 producing strain Streptomyces tsukubaensis L19, together wit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24255
更新日期：2016-04-07 00:00:00
abstract：:Clinical data have been equivocal and controversial as to the benefits to the brain and cognition of hormone therapy (HT) in postmenopausal women. Recent reevaluation of the role of estrogens proposed that HT may effectively prevent the deleterious effects of aging on cognition, and reduces the risks of dementia, incl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44917
更新日期：2017-03-21 00:00:00
abstract：:We report on the first realization of an ultrafast Airy beam optical parametric oscillator (OPO). By introducing intracavity cubic phase modulation to the resonant Gaussian signal in a synchronously-pumped singly-resonant OPO cavity and its subsequent Fourier transformation, we have generated 2-dimensional Airy beam i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30701
更新日期：2016-08-01 00:00:00
abstract：:Whether hybridization generates or erodes species diversity has long been debated, but to date most studies have been conducted at small taxonomic scales. Salamanders (order Caudata) represent a taxonomic order in which hybridization plays a prevalent ecological and evolutionary role. We employed a recently developed ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63378-w
更新日期：2020-04-16 00:00:00
abstract：:Coincident climatic and human effects strongly influence water-quality properties in estuarine-coastal ecosystems around the world. Time-series data for a number of ecosystems reveal high spatio-temporal variability superimposed on secular trends traceable to nutrient over-enrichment. In this paper, we present new ana...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43036-6
更新日期：2019-04-30 00:00:00
abstract：:The ecological consequences of habitat loss and fragmentation have been intensively studied on a broad, landscape-wide scale, but have less been investigated on the finer scale of individual habitat patches, especially when considering dynamic turnovers in the habitability of sites. We study changes to individual patc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47497-7
更新日期：2019-07-31 00:00:00
abstract：:Although it is clear that lipids are responsible for insulin resistance, it is poorly understood what types of lipids are involved. In this study, we verified the characteristic lipid species in skeletal muscle of a chronic exercise training model and a high-fat induced-obesity model. Three different lipidomics analys...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03267
更新日期：2013-11-20 00:00:00
abstract：:The lignite biodegradation procedure to produce water-soluble humic materials (WSHM) with a Penicillium stain was established by previous studies in our laboratory. This study researched the effects of WSHM on the growth of Bradyrhizobium liaoningense CCBAU05525 and its nodulation on soybean. Results showed that WSHM ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10836
更新日期：2015-06-08 00:00:00
abstract：:The coupling of the surface plasmon near-field into the sensing medium is key to the sensitivity of surface plasmon-based sensing devices. A low-index dielectric is necessary for the sensing medium to support a highly-penetrating surface plasmon evanescent field that extends well into the dielectric medium. The air-li...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38859-2
更新日期：2019-02-19 00:00:00
abstract：:Fluid overload (FO) has been shown to adversely affect multiple organs and survival in critically ill patients. Liver transplantation (LT) carries the risk of massive transfusion, which frequently results in FO. We investigated the association of postoperative weight gain with graft failure, early allograft dysfunctio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56543-3
更新日期：2019-12-27 00:00:00
abstract：:The prognostic factors for survival among patients with secondary osteosarcoma remain unclear. The aim of this study was to develop a practical nomogram for predicting cancer-specific survival (CSS) in patients with osteosarcoma as a secondary malignancy. The surveillance, epidemiology, and end results database was us...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69740-2
更新日期：2020-07-30 00:00:00
