Abstract：
:Atherosclerosis is an inflammatory disease in which dysfunction of mitochondria play an important role, and disorders of lipid management intensify this process. Agmatine, an endogenous polyamine formed by decarboxylation of arginine, exerts a protective effect on mitochondria and modulates fatty acid metabolism. We investigated the effect of exogenous agmatine on the development of atherosclerosis and changes in lipid profile in apolipoprotein E knockout (apoE-/-) mice. Agmatine caused an approximate 40% decrease of atherosclerotic lesions, as estimated by en face and cross-section methods with an influence on macrophage but not on smooth muscle content in the plaques. Agmatine treatment did not changed gelatinase activity within the plaque area. What is more, the action of agmatine was associated with an increase in the number of high density lipoproteins (HDL) in blood. Real-Time PCR analysis showed that agmatine modulates liver mRNA levels of many factors involved in oxidation of fatty acid and cholesterol biosynthesis. Two-dimensional electrophoresis coupled with mass spectrometry identified 27 differentially expressed mitochondrial proteins upon agmatine treatment in the liver of apoE-/- mice, mostly proteins related to metabolism and apoptosis. In conclusion, prolonged administration of agmatine inhibits atherosclerosis in apoE-/- mice; however, the exact mechanisms linking observed changes and elevations of HDL plasma require further investigation.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wiśniewska A,Olszanecki R,Totoń-Żurańska J,Kuś K,Stachowicz A,Suski M,Gębska A,Gajda M,Jawień J,Korbut Rdoi
10.3390/ijms18081706subject
Has Abstractpub_date
2017-08-04 00:00:00issue
8issn
1422-0067pii
ijms18081706journal_volume
18pub_type
杂志文章abstract：:Liver injuries caused by the use of exogenous compounds such as drugs, herbs, and alcohol are commonly well diagnosed using laboratory tests, toxin analyses, or eventually reactive intermediates generated during metabolic degradation of the respective chemical in the liver and subject to covalent binding by target pro...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21010212
更新日期：2019-12-27 00:00:00
abstract：:Protein splicing catalyzed by inteins utilizes many different combinations of amino-acid types at active sites. Inteins have been classified into three classes based on their characteristic sequences. We investigated the structural basis of the protein splicing mechanism of class 3 inteins by determining crystal struc...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218367
更新日期：2020-11-07 00:00:00
abstract：:Cannabidiol (CBD) is known for its vasorelaxant (including in the human pulmonary artery), anti-proliferative and anti-inflammatory properties. The aim of our study was to examine the potential preventive effect of chronic CBD administration (10 mg/kg/day for three weeks) on monocrotaline (MCT)-induced pulmonary hyper...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197077
更新日期：2020-09-25 00:00:00
abstract：:Cell proliferation is an important biological process during myogenesis. Tob1 encoded a member of the Tob/BTG family of anti-proliferative proteins. Our previous LongSAGE (Long Serial Analysis of Gene Expression) analysis suggested that Tob1 was differentially expressed during prenatal skeletal muscle development. In ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12074315
更新日期：2011-01-01 00:00:00
abstract：:As a conserved actin-regulating protein, CAP (adenylyl Cyclase-Associated Protein) functions to facilitate the rearrangement of the actin cytoskeleton. The ubiquitously expressed isoform CAP1 drives mammalian cell migration, and accordingly, most studies on the involvement of CAP1 in human cancers have largely been ba...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20112653
更新日期：2019-05-30 00:00:00
abstract：:Trihelix transcription factors are thought to feature a typical DNA-binding trihelix (helix-loop-helix-loop-helix) domain that binds specifically to the GT motif, a light-responsive DNA element. Members of the trihelix family are known to function in a number of processes in plants. Here, we characterize 20 trihelix f...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17020198
更新日期：2016-02-02 00:00:00
abstract：BACKGROUND:Carbapenems (ertapenem, imipenem, meropenem) are used to treat multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB), even if the published evidence is limited, particularly when it is otherwise difficult to identify the recommended four active drugs to be included in the regimen. N...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17030373
更新日期：2016-03-12 00:00:00
abstract：:Over 70 different genetic variants with a significant association with rheumatoid arthritis (RA) have been discovered. Anti-citrullination protein antibodies (ACPA)-positive RA variants are more well-defined than their ACPA-negative counterparts. The human leukocyte antigen, HLA-DRB1 locus remains the prime suspect in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms17060900
更新日期：2016-06-08 00:00:00
abstract：:This study evaluated the genetic diversity and population structures in a novel cotton germplasm collection comprising 132 diploids, including Glossypium klotzschianum and allotetraploid cotton accessions, including Glossypium barbadense, Glossypium darwinii, Glossypium tomentosum, Glossypium ekmanianum, and Glossypiu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082401
更新日期：2018-08-14 00:00:00
abstract：:In recent years it has become apparent that aminoacyl-tRNAs are not only crucial components involved in protein biosynthesis, but are also used as substrates and amino acid donors in a variety of other important cellular processes, ranging from bacterial cell wall biosynthesis and lipid modification to protein turnove...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms150814610
更新日期：2014-08-21 00:00:00
abstract：:Endothelial cells (ECs) that line the lumen of blood vessels are important players in blood vessel formation, and EC migration is a key component of the angiogenic process. Thus, identification of genes that are specifically or preferentially expressed in vascular ECs and in-depth understanding of their biological fun...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13044920
更新日期：2012-01-01 00:00:00
abstract：:A complex antioxidant system has been developed in mammals to relieve oxidative stress. However, excessive reactive species derived from oxygen and nitrogen may still lead to oxidative damage to tissue and organs. Oxidative stress has been considered as a conjoint pathological mechanism, and it contributes to initiati...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161125942
更新日期：2015-11-02 00:00:00
abstract：:Thirteen polyphenolics were isolated from fresh pods of Caesalpinia pulcherrima using various methods of column chromatography. The structures of these polyphenolics were elucidated as gallic acid (1), methyl gallate (2), 6-O-galloyl-d-glucoside (3), methyl 6-O-galloyl-β-d-glucoside (4), methyl 3,6-di-O-galloyl-α-d-gl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13056073
更新日期：2012-01-01 00:00:00
abstract：:The voltage-dependent anion channel (VDAC), a highly conserved major mitochondrial outer membrane protein, plays crucial roles in energy metabolism and metabolite transport. However, knowledge about the roles of the VDAC family in plants is limited. In this study, we investigated the expression pattern of VDAC1 in Ara...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14010701
更新日期：2013-01-04 00:00:00
abstract：:Cadmium at environmental concentrations is a risk factor for many diseases, including cardiovascular and neurodegenerative diseases, in which macrophages play an important role. The aim of this study was to evaluate the effects of cadmium at low environmental (nanomolar) concentrations on apoptotic processes in THP-1(...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160921410
更新日期：2015-09-07 00:00:00
abstract：:Currently, there is no three-dimensional structure of D-specific dehalogenase (DehD) in the protein database. We modeled DehD using ab initio technique, performed molecular dynamics (MD) simulation and docking of D-2-chloropropionate (D-2CP), D-2-bromopropionate (D-2BP), monochloroacetate (MCA), monobromoacetate (MBA)...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms131215724
更新日期：2012-11-26 00:00:00
abstract：:Acidovorax citrulli (A. citrulli) strains cause bacterial fruit blotch (BFB) in cucurbit crops and affect melon significantly. Numerous strains of the bacterium have been isolated from melon hosts globally. Strains that are aggressively virulent towards melon and diagnostic markers for detecting such strains are yet t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20112715
更新日期：2019-06-02 00:00:00
abstract：:Three kinds of new water-soluble polysaccharides (FA, FB and FC) were isolated from wild mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam by the classical method "water extraction and alcohol precipitation" and purified by column chromatography. The Mw of FA, FB and FC ranged from 5690 Da to 38,340 Da. The three poly...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030637
更新日期：2019-02-01 00:00:00
abstract：:Emodin (1,3,8-trihydroxy-6-methylanthraquinone) has been identified to have the potential to improve lung fibrosis and lung cancer. To avoid the liver and kidney toxicities and the fast metabolism of emodin, emodin-loaded polylactic acid microspheres (ED-PLA-MS) were prepared and their characteristics were studied. ED...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms15046241
更新日期：2014-04-11 00:00:00
abstract：:The retromer is a multiprotein complex conserved from yeast to humans, which is involved in intracellular protein trafficking and protein recycling. Selection of cargo proteins transported by the retromer depends on the core retromer subunit composed of the three vacuolar protein sorting (VPS) proteins, namely VPS26, ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20020362
更新日期：2019-01-16 00:00:00
abstract：:The potential for developing molecules of interest in preclinical preeclampsia from candidate genes that were discovered on gene expression microarray analysis has been challenged by limited access to additional first trimester trophoblast and decidual tissues. The question of whether these candidates encode secreted ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms161126023
更新日期：2015-11-13 00:00:00
abstract：:Cell death represents a basic biological paradigm that governs outcomes and long-term sequelae in almost every hepatic disease. Necroptosis is a common form of programmed cell death in the liver. Necroptosis can be activated by ligands of death receptors, which then interact with receptor-interactive protein kinases 1...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms22010066
更新日期：2020-12-23 00:00:00
abstract：BACKGROUND:Nowadays, the molecular mechanisms governing TSD (temperature-dependent sex determination) or GSD + TE (genotypic sex determination + temperature effects) remain a mystery in fish. METHODS:We developed three all-female families of Nile tilapia (Oreochromis niloticus), and the family with the highest male ra...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030689
更新日期：2018-02-28 00:00:00
abstract：:Diabetic retinopathy (DR), a sight-threatening neurovasculopathy, is the leading cause of irreversible blindness in the developed world. DR arises as the result of prolonged hyperglycemia and is characterized by leaky retinal vasculature, retinal ischemia, retinal inflammation, angiogenesis, and neovascularization. Th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20194755
更新日期：2019-09-25 00:00:00
abstract：:Fourier transform infrared (FTIR) microspectroscopy shows potential as a benign, objective and rapid tool to screen pluripotent and multipotent stem cells for clinical use. It offers a new experimental approach that provides a holistic measurement of macromolecular composition such that a signature representing the in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms140917453
更新日期：2013-08-26 00:00:00
abstract：:Prior evidence indicates that homocysteine plays a role in the development of metabolic syndrome (MetS). Methylenetetrahydrofolate reductase (MTHFR) C677T and methionine synthase reductase (MTRR) A66G polymorphisms are common genetic determinants of homocysteine levels. To investigate the associations of the MTHFR C67...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms151221687
更新日期：2014-11-25 00:00:00
abstract：:This study aims to reveal the potential relationship between 5-HT and oxidative stress in the organism. Our in vitro experiments in RIN-14B cells showed that anoxia leads the cells to the state of oxidative stress. Administration of exogenous 5-HT exacerbated this effect, whereas the inhibition of Tph1, LP533401 allev...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20061364
更新日期：2019-03-18 00:00:00
abstract：:Consumption of a high-fat diet (HFD) promotes the development of obesity, a disease resulting from an imbalance between energy intake and energy expenditure. Brown adipose tissue (BAT) has thermogenic capacity that burns calories to produce heat, and it is a potential target for the treatment and prevention of obesity...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19041095
更新日期：2018-04-06 00:00:00
abstract：:In a previous study on chromate toxicity, an increase in the 2Fe2S electron paramagnetic resonance (EPR) signal from mitochondria was found upon addition of chromate to human bronchial epithelial cells and bovine airway tissue ex vivo. This study was undertaken to show that a chromate-induced increase in the 2Fe2S EPR...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051143
更新日期：2019-03-06 00:00:00
abstract：:PCSK9 (Proprotein convertase subtilisin/kexin type 9) increases plasma cholesterol levels by promoting LDL receptor degradation. Current antibody inhibitors block the interaction between PCSK9 and LDL receptors, significantly decrease plasma cholesterol levels, and provide beneficial clinical outcomes. To reduce the a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19020640
更新日期：2018-02-24 00:00:00
