Abstract:
:Consumption of a high-fat diet (HFD) promotes the development of obesity, a disease resulting from an imbalance between energy intake and energy expenditure. Brown adipose tissue (BAT) has thermogenic capacity that burns calories to produce heat, and it is a potential target for the treatment and prevention of obesity. There is limited information regarding the impact of HFD on the BAT transcriptome. We hypothesized that HFD-induced obesity would lead to transcriptional regulation of BAT genes. RNA sequencing was used to generate global transcriptome profiles from BAT of lean mice fed with a low-fat diet (LFD) and obese mice fed with a HFD. Gene Ontology (GO) analysis identified increased expression of genes involved in biological processes (BP) related to immune responses, which enhanced molecular function (MF) in chemokine activity; decreased expression of genes involved in BP related to ion transport and muscle structure development, which reduced MF in channel and transporter activity and structural binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional pathway analysis indicated that pathways associated with innate immunity were enhanced by HFD, while pathways associated with muscle contraction and calcium signaling were suppressed by HFD. Collectively, these results suggest that diet-induced obesity changes transcriptomic signatures of BAT, leading to dysfunction involving inflammation, calcium signaling, ion transport, and cell structural development.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Cao J,Zhu Q,Liu L,Glazier BJ,Hinkel BC,Liang C,Shi Hdoi
10.3390/ijms19041095subject
Has Abstractpub_date
2018-04-06 00:00:00issue
4issn
1422-0067pii
ijms19041095journal_volume
19pub_type
杂志文章abstract::The development of new therapeutic options against Clostridioides difficile (C. difficile) infection is a critical public health concern, as the causative bacterium is highly resistant to multiple classes of antibiotics. Antimicrobial host-defense peptides (HDPs) are highly effective at simultaneously modulating the i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215289
更新日期:2019-10-24 00:00:00
abstract::Asymmetric dimethylarginine (ADMA) is an endogenous nitric oxide synthase (NOS) inhibitor known as a mediator of endothelial dysfunction and atherosclerosis. Circulating ADMA levels are correlated with cardiovascular risk factors such as hypercholesterolemia, arterial hypertension, diabetes mellitus, hyperhomocysteine...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15046391
更新日期:2014-04-15 00:00:00
abstract::Pathogen-induced reorganization of the host cell cytoskeleton is a common strategy utilized in host cell invasion by many facultative intracellular bacteria, such as Shigella, Listeria, enteroinvasive E. coli and Salmonella. Shigella is an enteroinvasive intracellular pathogen that preferentially infects human epithel...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms151018253
更新日期:2014-10-10 00:00:00
abstract::Safflower (Carthamus tinctorius L.), an important traditional Chinese medicine, is cultured widely for its pharmacological effects, but little is known regarding the genes related to the metabolic regulation of the safflower's yellow pigment. To investigate genes related to safflor yellow biosynthesis, 454 pyrosequenc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161025657
更新日期:2015-10-26 00:00:00
abstract::The ability to respond flexibly to changing environmental circumstances is a hallmark of goal-directed behavior, and compromised flexibility is associated with a wide range of psychiatric conditions in humans, such as addiction and stress-related disorders. To identify neural circuits and transmitter systems implicate...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20123098
更新日期:2019-06-25 00:00:00
abstract::Transcription factors play vital roles in the developmental processes of plants. The SQUAMOSA promoter binding protein (SBP) genes encode a family of plant-specific transcription factors and plays many crucial roles in plant development. In this study, 16 SBP-box gene family members were identified in Ziziphus jujuba ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081734
更新日期:2017-08-15 00:00:00
abstract::Several cellular insults can result in sperm DNA fragmentation either on one or both DNA strands. Oxidative damage, premature interruption of the apoptotic process and defects in DNA compaction during spermatogenesis are the main mechanisms that cause DNA breaks in sperm. The two-tailed Comet assay is the only techniq...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21113882
更新日期:2020-05-29 00:00:00
abstract::Mycobacterium immunogenum, a newly identified member of the Mycobacterium chelonae_M. abscessus complex is considered a potential etiological agent for hypersensitivity pneumonitis (HP) in machine workers exposed to contaminated metalworking fluid (MWF). This study investigated the biocidal efficacy of the frequently ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12010725
更新日期:2011-01-20 00:00:00
abstract::Cotton fibres, as single cells arising from the seed coat, can be classified as lint and fuzz according to their final length. Gossypium arboreum is a cultivated diploid cotton species and a potential donor of the A subgenome of the more widely grown tetraploid cottons. In this study, we performed genetic studies on o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051675
更新日期:2020-02-29 00:00:00
abstract::Monoterpenoids are a diverse class of natural products and contribute to the important varietal aroma of certain Vitis vinifera grape cultivars. Among the typical monoterpenoids, linalool exists in almost all grape varieties. A gene coding for a nerolidol/linalool (NES/LINS) synthase was evaluated in the role of linal...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151221992
更新日期:2014-12-01 00:00:00
abstract::Malignancy often arises from sophisticated defects in the intricate molecular mechanisms of cells, rendering a complicated molecular ground to effectively target cancers. Resistance toward cell death and enhancement of cell survival are the common adaptations in cancer due to its infinite proliferative capacity. Exist...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20020372
更新日期:2019-01-16 00:00:00
abstract::Endometriosis is a chronic, inflammatory, hormone-dependent disease characterized by histological lesions produced by the presence of endometrial tissue outside the uterine cavity. Despite the fact that an estimated 176 million women are affected worldwide by this gynecological disorder, risk factors that cause endome...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21249397
更新日期:2020-12-10 00:00:00
abstract::Transcription factor binding sites (TFBSs) play an important role in gene expression regulation. Many computational methods for TFBS prediction need sufficient labeled data. However, many transcription factors (TFs) lack labeled data in cell types. We propose a novel method, referred to as DANN_TF, for TFBS prediction...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20143425
更新日期:2019-07-12 00:00:00
abstract::The MAP kinase ERK5 contains an N-terminal kinase domain and a unique C-terminal tail including a nuclear localization signal and a transcriptional activation domain. ERK5 is activated in response to growth factors and stresses and regulates transcription at the nucleus by either phosphorylation or interaction with tr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062203
更新日期:2020-03-23 00:00:00
abstract::The homeostatic control of lipid metabolism is essential for many fundamental physiological processes. A deep understanding of its regulatory mechanisms is pivotal to unravel prospective physiopathological factors and to identify novel molecular targets that could be employed to design promising therapies in the manag...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041297
更新日期:2020-02-14 00:00:00
abstract::Genomic selection (GS) is a strategy to predict the genetic merits of individuals using genome-wide markers. However, GS prediction accuracy is affected by many factors, including missing rate and minor allele frequency (MAF) of genotypic data, GS models, trait features, etc. In this study, we used one wheat populatio...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041342
更新日期:2020-02-17 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is often diagnosed at an advanced stage and has a dismal prognosis. Nearly 10 years after the approval of cetuximab, anti-PD1/PD-L1 checkpoint inhibitors are the first drugs that have shown any survival benefit for the treatment on platinum-refractory recurrent/metastatic ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20215399
更新日期:2019-10-30 00:00:00
abstract::Senescence is the final stage of leaf development which is accompanied by highly coordinated and complicated reprogramming of gene expression. Genetic manipulation of leaf senescence in major crops including wheat has been shown to be able to increase stress tolerance and grain yield. NAC(No apical meristem (NAM), ATA...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21207672
更新日期:2020-10-16 00:00:00
abstract::Over the last decades a renewed interest in n-3 very long polyunsaturated fatty acids (PUFAs), derived mainly from fish oils in the human diet, has been observed because of their potential effects against cancer diseases, including breast carcinoma. These n-3 PUFAs mainly consist of eicosapentaenoic acid (EPA) and doc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21072279
更新日期:2020-03-26 00:00:00
abstract::Several studies have suggested that there is a link between membrane attack complex (MAC) deposition in the retina and the progression of diabetic retinopathy (DR). Our recent investigation demonstrated that circulating IgG-laden extracellular vesicles contribute to an increase in retinal vascular permeability in DR t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051693
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Widespread use of prostate specific antigen (PSA) in screening procedures allowed early identification of an increasing number of prostate cancers (PCas), mainly including indolent cancer. Availability of different therapeutic strategies which have a very different impact on the patient's quality of life sug...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21041184
更新日期:2020-02-11 00:00:00
abstract::Prodrug activator gene therapy mediated by murine leukemia virus (MLV)-based retroviral replicating vectors (RRV) was previously shown to be highly effective in killing glioma cells both in culture and in vivo. To avoid receptor interference and enable dual vector co-infection with MLV-RRV, we have developed another R...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041433
更新日期:2020-02-20 00:00:00
abstract::Accurate and sensitive determination of hematopoietic chimerism is a crucial diagnostic measure after allogeneic stem cell transplantation to monitor engraftment and potentially residual disease. Short tandem repeat (STR) amplification, the current "gold standard" for chimerism assessment facilitates reliable accuracy...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17091515
更新日期:2016-09-09 00:00:00
abstract::Prostate cancer (PC) is a frequent male malignancy and represents the second most diagnosed cancer in men. Since pre-cancerous lesions, i.e., the high-grade prostatic intraepithelial neoplasia (HGPIN), can be detected years before progression to PC, early diagnosis and chemoprevention are targeted strategies to reduce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15011433
更新日期:2014-01-21 00:00:00
abstract::Both positive and negative aspects of sport performance are currently considered. The aim of our study was to determine time- and intensity-dependent effects of a single exercise bout on redox and inflammatory status. The experiment was performed on 40 male Wistar rats subjected to treadmill running for 30 min with th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218071
更新日期:2020-10-29 00:00:00
abstract::Alpha-synuclein (SNCA) is a small membrane protein that plays an important role in neuro-psychiatric diseases. It is best known for its abnormal subcellular aggregation in Lewy bodies that serves as a hallmark of Parkinson's disease (PD). Due to the high comorbidity of PD with depression, we investigated the role of S...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20082029
更新日期:2019-04-25 00:00:00
abstract::Starting from dansyl-chloride, in reaction with 1,1-diphenylhydrazine and methoxyamine, two new fluorescent derivatives 1 and 2 were obtained and characterized by NMR, IR, UV-Vis, HR-MS, and fluorescence spectroscopy. The single-crystal X-ray structure was obtained for compound 2. Both compounds generate free radicals...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21103559
更新日期:2020-05-18 00:00:00
abstract::Mast cells (MC) are resident tissue cells found primarily at the interphase between tissues and the environment. These evolutionary old cells store large amounts of proteases within cytoplasmic granules, and one of the most abundant of these proteases is tryptase. To look deeper into the question of their in vivo targ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20205147
更新日期:2019-10-17 00:00:00
abstract::Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific bin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15058821
更新日期:2014-05-19 00:00:00
abstract::The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13033431
更新日期:2012-01-01 00:00:00