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Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR.

Abstract：

:What's on the label is not what's in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodities is highly desirable. In this field, DNA-based techniques have proved to be powerful tools to overcome qualitative challenges. However, is it possible to quantify the weight of biological materials with PCR? Therefore, a basic scientific question needs to be answered: what's the relationship between DNA content and the mass of biological materials? Is DNA content directly proportional to the mass of biological materials as most of the researchers previously thought? In this study, we firstly found that there exists a linear relation between DNA contents and the weight of biomaterials indeed when the analytical practices are fully controlled. In this case, the mass of targeted biomaterials in the highly processed commercial products can also be calculated by quantifying the species-specific DNA through classic real-time PCR with a good reproducibility.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Chen R,Wang J,Yuan Y,Deng Y,Lai X,Du F,Dong J,Huang X,Cui X,Tang Z

doi

10.1038/s41598-017-05083-9

subject

Has Abstract

pub_date

2017-07-06 00:00:00

pages

4774

issue

1

issn

2045-2322

pii

10.1038/s41598-017-05083-9

journal_volume

7

pub_type

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