Abstract：
:Aldosterone and the Mineralocorticoid Receptor (MR) control hydroelectrolytic homeostasis and alterations of mineralocorticoid signaling pathway are involved in the pathogenesis of numerous human diseases, justifying the need to decipher molecular events controlling MR expression level. Here, we show in renal cells that the RNA-Binding Protein, Human antigen R (HuR), plays a central role in the editing of MR transcript as revealed by a RNA interference strategy. We identify a novel Δ6 MR splice variant, which lacks the entire exon 6, following a HuR-dependent exon skipping event. Using isoform-specific TaqMan probes, we show that Δ6 MR variant is expressed in all MR-expressing tissues and cells and demonstrate that extracelullar tonicity regulates its renal expression. More importantly, this splice variant exerts dominant-negative effects on transcriptional activity of the full-length MR protein. Collectively, our data highlight a crucial role of HuR as a master posttranscriptional regulator of MR expression in response to osmotic stress. We demonstrate that hypotonicity, not only enhances MR mRNA stability, but also decreases expression of the Δ6 MR variant, thus potentiating renal MR signaling. These findings provide compelling evidence for an autoregulatory feedback loop for the control of sodium homeostasis through posttranscriptional events, likely relevant in renal pathophysiological situations.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Lema I,Amazit L,Lamribet K,Fagart J,Blanchard A,Lombès M,Cherradi N,Viengchareun Sdoi
10.1038/s41598-017-04838-8subject
Has Abstractpub_date
2017-07-06 00:00:00pages
4835issue
1issn
2045-2322pii
10.1038/s41598-017-04838-8journal_volume
7pub_type
杂志文章abstract：:The unconventional myosin MYO18A that contains a PDZ domain is required for muscle integrity during zebrafish development. However, the mechanism by which it functions in myofibers is not clear. The presence of a PDZ domain suggests that MYO18A may interact with other partners to perform muscle-specific functions. Her...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36768
更新日期：2016-11-08 00:00:00
abstract：:Rice breeding has achieved great productivity improvements by semi-dwarf varieties and hybrid vigour. Due to poor understanding of genetic basis of elite backbone varieties, the continuous increasing in rice yield still faces great challenges. Here, 52 elite rice varieties from three historical representative pedigree...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00415-1
更新日期：2017-03-22 00:00:00
abstract：:The application of CLSI and EUCAST guidelines led to many discrepancies. Various doubts have already appeared in preliminary stages of microbiological diagnostics of Haemophilus spp. A total of 87 H. parainfluenzae isolates were obtained from throat or nasopharyngeal swabs from adults 18 to 70 years old, both healthy ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68161-5
更新日期：2020-07-13 00:00:00
abstract：:Interactions of high-frequency radio waves (RF) with biological tissues are currently being investigated as a therapeutic platform for non-invasive cancer hyperthermia therapy. RF delivers thermal energy into tissues, which increases intra-tumoral drug perfusion and blood-flow. Herein, we describe an optical-based met...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43961
更新日期：2017-03-13 00:00:00
abstract：:Male infertility is a worldwide medical problem. Asthenozoospermia is a common cause of infertility. Epigenetic modifications of DNA and histones have been shown to influence human infertility, but no research has explored whether N(6)-methyladenosine (m(6)A) level in RNA is associated with asthenozoospermia. Here, we...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24345
更新日期：2016-04-13 00:00:00
abstract：:Composite materials have become widely used in engineering applications, in order to reduce the overall weight of structures while retaining their required strength. In this work, a composite material consisting of discontinuous glass fibers in a polypropylene matrix is studied at the microstructural level through cou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60368-w
更新日期：2020-02-27 00:00:00
abstract：:A novel characterization technique using both in situ reflection high-energy electron diffraction (RHEED) transmission mode and transmission electron microscopy (TEM) has been developed to investigate the growth behaviour of semiconductor nanostructures. RHEED employed in transmission mode enables the acquisition of s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19857-2
更新日期：2018-01-26 00:00:00
abstract：:The extracellular matrix (ECM) impacts stem cell differentiation, but identifying formulations supportive of differentiation is challenging in 3D models. Prior efforts involving combinatorial ECM arrays seemed intuitively advantageous. We propose an alternative that suggests reducing sample size and technological burd...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18705
更新日期：2015-12-21 00:00:00
abstract：:In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing. Additionally, no study has reported the possibility of two-color in-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78879-x
更新日期：2020-12-14 00:00:00
abstract：:Monoclonal antibodies (mAbs) are attractive therapeutics for treating a wide range of human disorders, and bind to the antigen through their complementarity-determining regions (CDRs). Small stable proteins containing structurally retained CDRs are promising alternatives to mAbs. In this report, we present a method to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57713-4
更新日期：2020-01-21 00:00:00
abstract：:Pancreatic ductal adenocarcinoma (PDAC) exists in a complex desmoplastic microenvironment. As part of it, pancreatic stellate cells (PSCs) provide a fibrotic niche, stimulated by a dynamic communication between activated PSCs and tumour cells. Investigating how PSCs contribute to tumour development and for identifying...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41740-x
更新日期：2019-03-28 00:00:00
abstract：:Rice (Oryza sativa L.) is one of the essential staple food crops and tillering, panicle branching and grain filling are three important traits determining the grain yield. Although miRNAs have been reported being regulating yield, no study has systematically investigated how miRNAs differentially function in high and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26438-w
更新日期：2018-05-31 00:00:00
abstract：:Hepcidin, a cysteine-rich peptide hormone, secreted mainly by the liver, plays a central role in iron metabolism regulation. Emerging evidence suggests that disordered iron metabolism is a risk factor for various types of diseases including cancers. However, it remains challenging to apply current mass spectrometry (M...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43756-9
更新日期：2019-05-13 00:00:00
abstract：:To date there are thirteen species validly assigned to the genus Anaerococcus. Most of the species in this genus are anaerobic and of human origin. Anaerococcus urinimassiliensis sp. nov., strain Marseille-P2143T is member of family Peptoniphilaceae, which was isolated from the urine of a 17-year-old boy affected by a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-82420-z
更新日期：2021-01-29 00:00:00
abstract：:Technological advances have made it possible to examine dental resin composites using 3D nanometer resolution. This investigation aims to characterize existing dental nano-hybrid and micro-hybrid resin composites through comparing and contrasting nano-computed tomography (nano-CT) with micro-CT and high-resolution SEM...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72599-y
更新日期：2020-09-23 00:00:00
abstract：:In inflammatory bowel disease (IBD), compromised restitution of the epithelial barrier contributes to disease severity. Owing to the complexity in the pathogenesis of IBD, a variety of factors have been implicated in its progress. In this study, we report a functional interaction between macroautophagy and Corticotrop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23342
更新日期：2016-03-18 00:00:00
abstract：:Dolphin morbillivirus (DMV) has been responsible for several outbreaks of systemic infection and has resulted in cetacean strandings in the Mediterranean. In August-October 2016, seven striped dolphins (Stenella coeruleoalba) stranded on the Sicilian coastline (Italy) tested positive for DMV. Tissue samples from brain...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46096-w
更新日期：2019-07-05 00:00:00
abstract：:Early detection and treatment of coronary artery disease (CAD) can reduce incidences of acute myocardial infarction. In this study, we determined the proper use of contributing risk factors and coronary artery calcium score (CACS) when screening asymptomatic patients with coronary arterial stenoses using coronary comp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17655-w
更新日期：2017-12-15 00:00:00
abstract：:We study a system of active particles with soft repulsive interactions that lead to an active cluster-crystal phase in two dimensions. We use two different modelizations of the active force - Active Brownian particles (ABP) and Ornstein-Uhlenbeck particles (AOUP) - and focus on analogies and differences between them. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52420-1
更新日期：2019-11-13 00:00:00
abstract：:The profiles of time-contrast (TC) -curves from popular MRI injectors derived at the injection site of the attached tube-line system were compared. Variations of TC-profiles were previously reported to potentially influence image quality in time critical MRI measurements. TC-curves from five injectors obtained during ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76536-x
更新日期：2020-11-11 00:00:00
abstract：:Given the complex nature of the interaction between gas and solid atoms, the development of nanoscale science and technology has engendered a need for further understanding of gas transport behavior through nanopores and more tractable models for large-scale simulations. In the present paper, we utilize molecular dyna...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23629
更新日期：2016-03-29 00:00:00
abstract：:Despite the importance of urban trees, their growth reaction to climate change and to the urban heat island effect has not yet been investigated with an international scope. While we are well informed about forest growth under recent conditions, it is unclear if this knowledge can be simply transferred to urban enviro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14831-w
更新日期：2017-11-13 00:00:00
abstract：:Sensory representations are typically endowed with intrinsic noise, leading to variability and inaccuracies in perceptual responses. The Bayesian framework accounts for an optimal strategy to deal with sensory-motor uncertainty, by combining the noisy sensory input with prior information regarding the distribution of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34512-6
更新日期：2018-11-06 00:00:00
abstract：:A new approach to estimate the Shannon entropy of a long-range correlated sequence is proposed. The entropy is written as the sum of two terms corresponding respectively to power-law (ordered) and exponentially (disordered) distributed blocks (clusters). The approach is illustrated on the 24 human chromosome sequences...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02721
更新日期：2013-01-01 00:00:00
abstract：:Previously, we cloned a new gene termed 'tongue cancer resistance-associated protein 1' (TCRP1), which modulates tumorigenesis, enhances cisplatin (cDDP) resistance in cancers, and may be a potential target for reversing drug resistance. However, the mechanisms for regulating TCRP1 expression remain unclear. Herein, w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03763-0
更新日期：2017-06-16 00:00:00
abstract：:Acoustic structure quantification (ASQ) is a recently developed technique widely used for detecting liver fibrosis. Ultrasound Nakagami parametric imaging based on the Nakagami distribution has been widely used to model echo amplitude distribution for tissue characterization. We explored the feasibility of using ultra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33075
更新日期：2016-09-08 00:00:00
abstract：:Catharanthus roseus is a commercial source for anti-cancer terpenoid indole alkaloids (TIAs: vincristine and vinblastine). Inherent levels of these TIAs are very low, hence research studies need to focus on enhancing their levels in planta. Since primary metabolism provides precursors for specialized-metabolism, eleva...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33415-w
更新日期：2018-10-10 00:00:00
abstract：:A subset of the family of fluorescent proteins are the non-fluorescent chromoproteins which are promising probe molecules for use in photoacoustic imaging and as acceptor chromophores in Förster resonance energy transfer (FRET)-based biosensors. Typical approaches for fluorescent protein optimization by screening of l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22129
更新日期：2016-03-01 00:00:00
abstract：:Chemotherapy can cause cachexia, which consists of weight loss associated with muscle atrophy. The exact mechanisms underlying this skeletal muscle toxicity are largely unknown and co-therapies to attenuate chemotherapy-induced side effects are lacking. By using a rat model of cisplatin-induced cachexia, we here chara...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13504-y
更新日期：2017-10-12 00:00:00
abstract：:Cold agglutinin disease (CAD) is a rare form of autoimmune haemolytic anaemia, and because of its rareness, there is no standard treatment for CAD patients. We retrospectively analysed the response to rituximab-containing therapy in CAD patients at our hospital. All patients received rituximab-containing therapy for a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69465-2
更新日期：2020-07-29 00:00:00
