当前位置： SCI文献检索 > JOURNAL OF ETHNOPHARMACOLOGY期刊下所有文献 > Ulmus davidiana Nakai induces apoptosis and autophagy on non-small cell lung cancer cells.

Ulmus davidiana Nakai induces apoptosis and autophagy on non-small cell lung cancer cells.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Ulmus davidiana Nakai (UDN) is frequently used in the treatment of cancer in traditional oriental medicine. Although several reports indicate that UDN has inhibitory effects in some cancers, there has been no report on the inhibitory effects of UDN via both autophagy and apoptosis. MATERIALS AND METHODS:Cytotoxicity induced by UDN in human non-small cell lung cancer (NSCLC) H-1299 and H-460 cell lines was evaluated using the 2, 3-Bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) assay and trypan blue exclusion assay. Induction of apoptosis was also investigated using Hoechst staining and annexin-V binding assay and was confirmed with western blot analysis. Induction of autophagy was investigated through observation of autophagy vacuoles under inverted phase-contrast microscopy and was confirmed by observing the formation of autophagy vacuoles under a fluorescence microscope using monodansylcadaverine (MDC) staining and western blot analysis. The in vivo anti-tumorigenic effect of UDN was investigated in an athymic nude mouse xenograft model using H-1299 NSCLC cells. RESULTS:UDN exhibited a marked inhibitory effect on cell growth in H-1299 and H-460 human NSCLC cell lines in a dose- and time-dependent manner in vitro and in vivo. It induced not only apoptosis, but also autophagy in both H-1299 and H-460 cells in a dose-dependent manner. UDN-mediated autophagy led to the accumulation of autophagosome, resulting in apoptosis induction and cell death. CONCLUSIONS:From our current knowledge, we are the first to demonstrate that UDN has the potential to induce both autophagy and apoptosis in H-1299 and H-460 human NSCLC cell lines. We suggest that UDN can be considered a potential candidate for lung cancer-specific chemotherapy with efficacy as a cytotoxic agent.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Hong SO,Choi IK,Jeong W,Lee SR,Sung HJ,Hong SS,Seo JH

doi

10.1016/j.jep.2017.03.009

subject

Has Abstract

pub_date

2017-04-18 00:00:00

pages

1-11

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(16)32364-9

journal_volume

202

pub_type

杂志文章

在线工具

Ulmus davidiana Nakai induces apoptosis and autophagy on non-small cell lung cancer cells.