Abstract:
:Vascular cell adhesion molecule-1 (VCAM-1) is closely associated with tumor progression and metastasis. However, the relevance and role of VCAM-1 in lung cancer have not been clearly elucidated. In this study, we found that VCAM-1 was highly overexpressed in lung cancer tissue compared with that of normal lung tissue, and high VCAM-1 expression correlated with poor survival in lung cancer patients. VCAM-1 knockdown reduced migration of A549 human lung cancer cells into Matrigel, and competitive blocking experiments targeting the Ig-like domain 6 of VCAM-1 (VCAM-1-D6) demonstrated that the VCAM-1-D6 domain was critical for VCAM-1 mediated A549 cell migration into Matrigel. Next, we developed a human monoclonal antibody specific to human and mouse VCAM-1-D6 (VCAM-1-D6 huMab), which was isolated from a human synthetic antibody library using phage display technology. Finally, we showed that VCAM-1-D6 huMab had a nanomolar affinity for VCAM-1-D6 and that it potently suppressed the migration of A549 and NCI-H1299 lung cancer cell lines into Matrigel. Taken together, these results suggest that VCAM-1-D6 is a key domain for regulating VCAM-1-mediated lung cancer invasion and that our newly developed VCAM-1-D6 huMab will be a useful tool for inhibiting VCAM-1-expressing lung cancer cell invasion.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Kim MR,Jang JH,Park CS,Kim TK,Kim YJ,Chung J,Shim H,Nam IH,Han JM,Lee Sdoi
10.3390/ijms18030566subject
Has Abstractpub_date
2017-03-06 00:00:00issue
3issn
1422-0067pii
ijms18030566journal_volume
18pub_type
杂志文章abstract::Intracranial malignancies, such as primary brain cancers and brain-localized metastases derived from peripheral cancers, are particularly difficult to treat with therapeutic agents, because the blood-brain barrier (BBB) effectively minimizes brain entry of the vast majority of agents arriving from the systemic circula...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123905
更新日期:2018-12-06 00:00:00
abstract::Cancers are heterogeneous at the cell level, and the mechanisms leading to cancer heterogeneity could be clonal evolution or cancer stem cells. Cancer stem cells are resistant to most anti-cancer treatments and could be preferential targets to reverse this resistance, either targeting stemness pathways or cancer stem ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19124036
更新日期:2018-12-13 00:00:00
abstract::Esterification of starch was carried out to expand the usefulness of starch for a myriad of industrial applications. Lipase B from Candida antarctica, immobilized on macroporous acrylic resin (Novozym 435), was used for starch esterification in two reaction systems: micro-solvent system and solvent-free system. The es...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13067226
更新日期:2012-01-01 00:00:00
abstract::NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in Malus baccata(...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041198
更新日期:2020-02-11 00:00:00
abstract::Over the last decades a renewed interest in n-3 very long polyunsaturated fatty acids (PUFAs), derived mainly from fish oils in the human diet, has been observed because of their potential effects against cancer diseases, including breast carcinoma. These n-3 PUFAs mainly consist of eicosapentaenoic acid (EPA) and doc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21072279
更新日期:2020-03-26 00:00:00
abstract::The introduction of glyphosate-resistant (GR) crops revolutionized weed management; however, the improper use of this technology has selected for a wide range of weeds resistant to glyphosate, referred to as superweeds. We characterized the high glyphosate resistance level of an Amaranthus hybridus population (GRH)-a ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20102396
更新日期:2019-05-15 00:00:00
abstract::Killer immunoglobulin-like receptor (KIR) 2DL4 (CD158d) was previously thought to be a human NK cell-specific protein. Mast cells are involved in allergic reactions via their KIT-mediated and FcɛRI-mediated responses. We recently detected the expression of KIR2DL4 in human cultured mast cells established from peripher...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21030954
更新日期:2020-01-31 00:00:00
abstract::The domain of unknown function 26 (DUF26), harboring a conserved cysteine-rich motif (C-X8-C-X2-C), is unique to land plants. Several cysteine-rich repeat proteins (CRRs), belonging to DUF26-containing proteins, have been implicated in the defense against fungal pathogens in ginkgo, cotton, and maize. However, little ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165698
更新日期:2020-08-09 00:00:00
abstract::Ca2+ signaling has been involved in controling critical cellular functions such as activation of proteases, cell death, and cell cycle control. The endoplasmatic reticulum plays a significant role in Ca2+ storage inside the cell, but mitochondria have long been recognized as a fundamental Ca2+ pool. Protozoan parasite...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22010469
更新日期:2021-01-05 00:00:00
abstract::Hyperpolarization-activated calcium channels (HACCs) are found in the plasma membrane and tonoplast of many plant cell types, where they have an important role in Ca2+-dependent signalling. The unusual gating properties of HACCs in plants, i.e., activation by membrane hyperpolarization rather than depolarization, dict...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113771
更新日期:2020-05-27 00:00:00
abstract::A variety of Magnetic Resonance (MR) and nuclear medicine (NM) techniques have been used in symptomatic and presymptomatic SCA2 gene carriers to explore,in vivo, the physiopathological biomarkers of the neurological dysfunctions characterizing the associated progressive disease that presents with a cerebellar syndrome...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21031020
更新日期:2020-02-04 00:00:00
abstract::We would like to submit the following correction to the published paper [1], the reason for this action is that the data in Table 3 were reanalyzed by one more accurate statistic method: On page 12, the sentence of paragraph three "OR and 95% CI were calculated by limited backward-LR (likelihood ratio) logistic regres...
journal_title:International journal of molecular sciences
pub_type: 已发布勘误
doi:10.3390/ijms18091899
更新日期:2017-09-04 00:00:00
abstract::Genomic selection (GS) is a strategy to predict the genetic merits of individuals using genome-wide markers. However, GS prediction accuracy is affected by many factors, including missing rate and minor allele frequency (MAF) of genotypic data, GS models, trait features, etc. In this study, we used one wheat populatio...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21041342
更新日期:2020-02-17 00:00:00
abstract::Keratinocytes are non-professional immune cells contributing actively to innate immune responses partially by reacting to a wide range of molecular patterns by activating pattern recognition receptors. Cytosolic nucleotide fragments as pathogen- or self-derived trigger factors are activating inflammasomes and inducing...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030774
更新日期:2018-03-08 00:00:00
abstract::Sirtuins (SIRTs) are class III histone deacetylases (HDACs) that play important roles in aging and a wide range of cellular functions. Sirtuins are crucial to numerous biological processes, including proliferation, DNA repair, mitochondrial energy homeostasis, and antioxidant activity. Mammals have seven different sir...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21186686
更新日期:2020-09-12 00:00:00
abstract::This study evaluated frequency-dependent effects of extremely low frequency electromagnetic fields (ELF-EMFs) on melanogenesis by melanocytes in vitro. Melanocytes were exposed to 2 mT EMFs at 30-75 Hz for 3 days before melanogenesis was examined. Exposure to ELF-EMFs at 50 and 60 Hz induced melanogenic maturation wit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18102120
更新日期:2017-10-11 00:00:00
abstract::In order to use cyanobacteria for the biological production of hydrogen, it is important to thoroughly study the function and the regulation of the hydrogen-production machine in order to better understand its role in the global cell metabolism and identify bottlenecks limiting H2 production. Most of the recent advanc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms151119938
更新日期:2014-10-31 00:00:00
abstract::Latent transforming growth factor-β-1 binding protein-2 (LTBP-2) belongs to the LTBP-fibrillin superfamily of extracellular proteins. Unlike other LTBPs, LTBP-2 does not covalently bind transforming growth factor-β1 (TGF-β1) but appears to be implicated in the regulation of TGF-β1 bioactivity, although the mechanisms ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18102114
更新日期:2017-10-09 00:00:00
abstract::Apolipoprotein E (apoE) is mainly secreted by hepatocytes and incorporated into most plasma lipoproteins. Macrophages, which accumulate cholesterol and are critical for the development of the atherosclerotic plaque, are also an important, albeit smaller, apoE source. Distal regulatory elements control cell-specific ac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20061471
更新日期:2019-03-23 00:00:00
abstract::The binding of epidermal growth factor (EGF) to EGF receptor (EGFR) stimulates cell mitogenesis and survival through various signalling cascades. EGF also stimulates rapid EGFR endocytosis and its eventual degradation in lysosomes. The immediate events induced by ligand binding include receptor dimerization, activatio...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17081200
更新日期:2016-07-25 00:00:00
abstract:BACKGROUND:The molecular signature of endometrial receptivity still remains barely understood, especially when focused on the possible benefit of therapeutical interventions and implantation-related pathologies. Therefore, the protein composition of tissue and isolated primary cells (endometrial stromal cells, ESCs) fr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20020425
更新日期:2019-01-19 00:00:00
abstract::Malignancy often arises from sophisticated defects in the intricate molecular mechanisms of cells, rendering a complicated molecular ground to effectively target cancers. Resistance toward cell death and enhancement of cell survival are the common adaptations in cancer due to its infinite proliferative capacity. Exist...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20020372
更新日期:2019-01-16 00:00:00
abstract::The present work provides a comprehensive overview of the recent progress of research work toward developing new one dimensional (1-D) ceria (CeO(2)) nanomaterials. The review has been classified into three parts: the preparation procedures with identification of the existing different dimensional ceria nanomaterials,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms11093226
更新日期:2010-09-13 00:00:00
abstract::Drought adversely affects crop production across the globe. The root system immensely contributes to water management and the adaptability of plants to drought stress. In this study, drought-induced phenotypic and transcriptomic responses of two contrasting chickpea (Cicer arietinum L.) genotypes were compared at the ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051781
更新日期:2020-03-05 00:00:00
abstract::Of the multiple sources of reactive oxygen species (ROS) in the blood vessel, NADPH oxidases are the primary source. Whereas several studies have implicated NADPH oxidases in the initiation of atherosclerosis, their roles in disease progression are incompletely understood. Our objective was to determine the potential ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140817017
更新日期:2013-08-19 00:00:00
abstract::The olfactory system, composed of the olfactory organs and the olfactory bulb, allows organisms to interact with their environment and through the detection of odor signals. Olfaction mediates behaviors pivotal for survival, such as feeding, mating, social behavior, and danger assessment. The olfactory organs are dire...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20071639
更新日期:2019-04-02 00:00:00
abstract::Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation en...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020371
更新日期:2020-01-07 00:00:00
abstract::It has been increasingly recognized that SNAIL1 and SNAIL2, as major EMT-inducers, might also be involved in drug resistance of cancer cells. We sought to determine a relation between SNAIL1/2, E-cadherin and N-cadherin expression, as well as ovarian cancer cells' resistance to cisplatin and EMT markers' level. Thus, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020980
更新日期:2021-01-19 00:00:00
abstract::The paper presents a detailed study of the biological effects of two amino acid hydroxyurea derivatives that showed selective antiproliferative effects in vitro on the growth of human tumor cell line SW620. Tested compounds induced cell cycle perturbations and apoptosis. Proteins were identified by proteomics analyses...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141223654
更新日期:2013-12-04 00:00:00
abstract::Cypermethrin is one of the most highly effective synthetic pyrethroid insecticides. The toxicity of cypermethrin to the reproductive and nervous systems has been well studied. However, little is known about the toxic effect of cypermethrin on immune cells such as macrophages. Here, we investigated the cytotoxicity of ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17060885
更新日期:2016-06-17 00:00:00