Activation of contact-dependent antibacterial tRNase toxins by translation elongation factors.

abstract：:Synthesis of mouse metallothionein (MT)-I and MT-II is transcriptionally induced by the synthetic glucocorticoid, dexamethasone (DEX) or both in vivo as well as in numerous cell lines. However, the location(s) of a glucocorticoid response element (GRE) has not been described. The observation that a marked MT-I gene, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly EJ,Sandgren EP,Brinster RL,Palmiter RD

更新日期：1997-09-16 00:00:00

abstract：:The B cell developmental pathway represents a leading system for the analysis of regulatory circuits that orchestrate cell fate specification and commitment. Considerable progress has been achieved within the past decade in the identification and genetic analysis of various regulatory components. These components incl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singh H,Medina KL,Pongubala JM

更新日期：2005-04-05 00:00:00

abstract：:Understanding nuclear receptor signaling in vivo would be facilitated by an efficient methodology to determine where a nuclear receptor is active. Herein, we present a feedback-inducible expression system in transgenic mice to detect activated nuclear receptor effector proteins by using an inducible reporter gene. Wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mata De Urquiza A,Solomin L,Perlmann T

更新日期：1999-11-09 00:00:00

abstract：:Systemic delivery of bioactive molecules in the CNS is hampered by the blood-brain barrier, which has bottlenecked noninvasive physiological study of the brain and the development of CNS drugs. Here we report that irradiation with an ultrashort pulsed laser to the blood vessel wall induces transient leakage of blood p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi M,Ku T,Chong K,Yoon J,Choi C

更新日期：2011-05-31 00:00:00

abstract：:The ionophores A23187 and bromolasalocid (Br-X537A) acted synergistically in translocating Ca(2+) from an aqueous into an organic immiscible phase or in mediating Ca(2+) transport across the organic phase, the effects obtained in the simultaneous presence of both ionophores being greater than those expected from a sum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deleers M,Gelbcke M,Malaisse WJ

更新日期：1981-01-01 00:00:00

abstract：:Cyclic electron flow (CEF) around photosystem I is thought to balance the ATP/NADPH energy budget of photosynthesis, requiring that its rate be finely regulated. The mechanisms of this regulation are not well understood. We observed that mutants that exhibited constitutively high rates of CEF also showed elevated prod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strand DD,Livingston AK,Satoh-Cruz M,Froehlich JE,Maurino VG,Kramer DM

更新日期：2015-04-28 00:00:00

abstract：:Cyclic GMP (cGMP) is an important second messenger in eukaryotes. It is formed by guanylyl cyclases (GCs), members of the nucleotidyl cyclases class III, which also comprises adenylyl cyclases (ACs) from most organisms. To date, no structures of eukaryotic GCs are available, and all bacterial class III proteins were f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rauch A,Leipelt M,Russwurm M,Steegborn C

更新日期：2008-10-14 00:00:00

abstract：:Peptide-protein interactions are important mediators of cellular-signaling events. Consensus binding motifs (also known as short linear motifs) within these contacts underpin molecular recognition, yet have poor pharmacological properties as discrete species. Here, we present methods to transform intact peptides into ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lawson KV,Rose TE,Harran PG

更新日期：2013-10-01 00:00:00

abstract：:Successful adaptation to changes in an animal's emotional and motivational environment depends on behavioral flexibility accompanied by changes in bodily responses, e.g., autonomic and endocrine, which support the change in behavior. Here, we identify the orbitofrontal cortex (OFC) as pivotal in the flexible regulatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reekie YL,Braesicke K,Man MS,Roberts AC

更新日期：2008-07-15 00:00:00

abstract：:Myelodysplastic syndromes (MDS) are among the most frequent hematologic malignancies. Patients have a short survival and often progress to acute myeloid leukemia. The diagnosis of MDS can be difficult; there is a paucity of molecular markers, and the pathophysiology is largely unknown. Therefore, we conducted a multic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aivado M,Spentzos D,Germing U,Alterovitz G,Meng XY,Grall F,Giagounidis AA,Klement G,Steidl U,Otu HH,Czibere A,Prall WC,Iking-Konert C,Shayne M,Ramoni MF,Gattermann N,Haas R,Mitsiades CS,Fung ET,Libermann TA

更新日期：2007-01-23 00:00:00

abstract：:The present investigation studied the influence of the blastocyst's state of activity on the "window" of implantation in the receptive uterus in the mouse. The receptive state of the uterus is defined as the limited time when the uterine milieu is favorable to blastocyst acceptance and implantation. In the mouse, impl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paria BC,Huet-Hudson YM,Dey SK

更新日期：1993-11-01 00:00:00

abstract：:The mechanisms regulating clonal expansion and contraction of T cells in response to immunization remain to be identified. A recent study established that there was a log-linear relation between CD4 T-cell precursor number (PN) and factor of expansion (FE), with a slope of ∼-0.5 over a range of 3-30,000 precursors per...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bocharov G,Quiel J,Luzyanina T,Alon H,Chiglintsev E,Chereshnev V,Meier-Schellersheim M,Paul WE,Grossman Z

更新日期：2011-02-22 00:00:00

abstract：:We describe a quantum mechanics/molecular mechanics investigation of the guanidinoacetate methyltransferase catalyzed reaction, which shows that proton transfer from guanidinoacetate (GAA) to Asp-134 and methyl transfer from S-adenosyl-L-methionine (AdoMet) to GAA are concerted. By self-consistent-charge density funct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang X,Bruice TC

更新日期：2006-10-31 00:00:00

abstract：:Specific binding properties of the tritium-labeled delta opiate receptor agonist [3H][2-D-penicillamine, 5-D-penicillamine]enkephalin [( 3H][D-Pen2, D-Pen5]enkephalin) were characterized in the rat brain and in a mouse neuroblastoma-rat glioma hybrid cell line (NG 108-15). Saturation isotherms of [3H][D-Pen2, D-Pen5]e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama K,Gee KW,Mosberg HI,Hruby VJ,Yamamura HI

更新日期：1985-04-01 00:00:00

abstract：:The uncatalyzed reactions of O(2) (S = 1) with organic substrates (S = 0) are thermodynamically favorable but kinetically slow because they are spin-forbidden and the one-electron reduction potential of O(2) is unfavorable. In nature, many of these important O(2) reactions are catalyzed by metalloenzymes. In the case ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pau MY,Lipscomb JD,Solomon EI

更新日期：2007-11-20 00:00:00

abstract：:Transcription in eukaryotic nuclei is carried out by DNA-dependent RNA polymerases I, II, and III. Human RNA polymerase III (Pol III) transcribes small untranslated RNAs that include tRNAs, 5S RNA, U6 RNA, and some microRNAs. Increased Pol III transcription has been reported to accompany or cause cell transformation. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haurie V,Durrieu-Gaillard S,Dumay-Odelot H,Da Silva D,Rey C,Prochazkova M,Roeder RG,Besser D,Teichmann M

更新日期：2010-03-02 00:00:00

abstract：:The substrate specificity of the catalytic subunit of rabbit skeletal muscle 3': 5'-cyclic AMP-dependent protein kinase (EC 2.7.1.37; ATP: protein phosphotransferase) has been studied using the synthetic peptide Arg-Gly-Tyr-Ser-Leu-Gly corresponding to the sequence around serine 24, a phosphorylation site in reduced, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kemp BE,Benjamini E,Krebs EG

更新日期：1976-04-01 00:00:00

abstract：:What are the forces that shape the structure of prokaryotic genomes: the order of genes, their proximity, and their orientation? Coregulation and coordinated horizontal gene transfer are believed to promote the proximity of functionally related genes and the formation of operons. However, forces that influence the str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kolesov G,Wunderlich Z,Laikova ON,Gelfand MS,Mirny LA

更新日期：2007-08-28 00:00:00

abstract：:The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Su SS,Modrich P

更新日期：1986-07-01 00:00:00

abstract：:The Ca(2+) channel alpha(1A)-subunit is a voltage-gated, pore-forming membrane protein positioned at the intersection of two important lines of research: one exploring the diversity of Ca(2+) channels and their physiological roles, and the other pursuing mechanisms of ataxia, dystonia, epilepsy, and migraine. alpha(1A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jun K,Piedras-Rentería ES,Smith SM,Wheeler DB,Lee SB,Lee TG,Chin H,Adams ME,Scheller RH,Tsien RW,Shin HS

更新日期：1999-12-21 00:00:00

abstract：:Antisense oligodeoxyribonucleotides targeted to the epidermal growth factor (EGF) receptor were encapsulated into liposomes linked to folate via a polyethylene glycol spacer (folate-PEG-liposomes) and efficiently delivered into cultured KB cells via folate receptor-mediated endocytosis. The oligonucleotides were a pho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang S,Lee RJ,Cauchon G,Gorenstein DG,Low PS

更新日期：1995-04-11 00:00:00

abstract：:Androgen independence is responsible for most prostate cancer lethality, yet currently there are no effective clinical treatments. We have been investigating the mechanisms underlying androgen-independent prostate cancer in Nkx3.1;Pten mutant mice, which display salient features of the disease, including a requirement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao H,Ouyang X,Banach-Petrosky WA,Gerald WL,Shen MM,Abate-Shen C

更新日期：2006-09-26 00:00:00

abstract：:ApcMin (Min, multiple intestinal neoplasia) is a point mutation in the murine homolog of the APC gene. Min/+ mice develop multiple intestinal adenomas, as do humans carrying germ-line mutations in APC. Female mice carrying Min are also prone to develop mammary tumors. Min/+ mammary glands are more sensitive to chemica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moser AR,Mattes EM,Dove WF,Lindstrom MJ,Haag JD,Gould MN

更新日期：1993-10-01 00:00:00

abstract：:We previously demonstrated that the B chain of platelet-derived growth factor (PDGF-B) is transcribed in human atherosclerotic plaques, indicating that production of growth factors within plaques could occur during atherogenesis. However, since atherosclerotic plaques are composed of several cell types and three of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrett TB,Benditt EP

更新日期：1988-04-01 00:00:00

abstract：:Optical waveguides simultaneously transport light at different colors, forming the basis of fiber-optic telecommunication networks that shuttle data in dozens of spectrally separated channels. Here, we reimagine this wavelength division multiplexing (WDM) paradigm in a novel context--the differentiated detection and i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozcelik D,Parks JW,Wall TA,Stott MA,Cai H,Parks JW,Hawkins AR,Schmidt H

更新日期：2015-10-20 00:00:00

abstract：:A reaction cycle for electron-transportdriven proton pumps is proposed. It includes two distinct conformational states of the pump protein in which the primary electron acceptor has different reduction potentials. This has as an unavoidable consequence that the steady-state rate equation for the catalytic reaction dri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brzezinski P,Malmström BG

更新日期：1986-06-01 00:00:00

abstract：:Regulation of the sterol-synthesizing mevalonate pathway occurs in part through feedback-regulated endoplasmic reticulum degradation of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R). In yeast, the Hmg2p isozyme of HMG-R is regulated in this manner. We have tested the involvement of ubiquitination in the regulated d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hampton RY,Bhakta H

更新日期：1997-11-25 00:00:00

abstract：:A remarkable property of tetrapod bone is its ability to detect and remodel areas where damage has accumulated through prolonged use. This process, believed vital to the long-term health of bone, is considered to be initiated and orchestrated by osteocytes, cells within the bone matrix. It is therefore surprising that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Atkins A,Dean MN,Habegger ML,Motta PJ,Ofer L,Repp F,Shipov A,Weiner S,Currey JD,Shahar R

更新日期：2014-11-11 00:00:00

abstract：:A vaccine capable of stimulating protective antiviral antibody responses is needed to curtail the global AIDS epidemic caused by HIV-1. Although rarely elicited during the course of natural infection or upon conventional vaccination, the membrane-proximal ectodomain region (MPER) of the HIV-1 glycoprotein of M(r) 41,0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song L,Sun ZY,Coleman KE,Zwick MB,Gach JS,Wang JH,Reinherz EL,Wagner G,Kim M

更新日期：2009-06-02 00:00:00

abstract：:The synaptic vesicle membrane protein synaptotagmin (tagmin) is essential for fast, calcium-dependent, neurotransmitter release and is likely to be the calcium sensor for exocytosis, because of its many calcium-dependent properties. Polyphosphoinositides are needed for exocytosis, but it has not been known why. We now...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schiavo G,Gu QM,Prestwich GD,Söllner TH,Rothman JE

更新日期：1996-11-12 00:00:00