Abstract:
:Systemic delivery of bioactive molecules in the CNS is hampered by the blood-brain barrier, which has bottlenecked noninvasive physiological study of the brain and the development of CNS drugs. Here we report that irradiation with an ultrashort pulsed laser to the blood vessel wall induces transient leakage of blood plasma without compromising vascular integrity. By combining this method with a systemic injection, we delivered target molecules in various tissues, including the brain cortex. This tool allows minimally invasive local delivery of chemical probes, nanoparticles, and viral vectors into the brain cortex. Furthermore, we demonstrated astrocyte-mediated vasodilation in vivo without opening the skull, using this method to load a calcium indicator in conjunction with label-free photoactivation of astrocytes.
journal_name
Proc Natl Acad Sci U S Aauthors
Choi M,Ku T,Chong K,Yoon J,Choi Cdoi
10.1073/pnas.1018790108subject
Has Abstractpub_date
2011-05-31 00:00:00pages
9256-61issue
22eissn
0027-8424issn
1091-6490pii
1018790108journal_volume
108pub_type
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