Abstract:
:The aim of the present study was to screen gastric cancer (GC) tissue and adjacent tissue for differences in mRNA and circular (circRNA) expression, to analyze the differences in circRNA and mRNA expression, and to investigate the circRNA expression in gastric carcinoma and its mechanism. circRNA and mRNA differential expression profiles generated using Agilent microarray technology were analyzed in the GC tissues and adjacent tissues. qRT-PCR was used to verify the differential expression of circRNAs and mRNAs according to the interactions between circRNAs and miRNAs as well as the possible existence of miRNA and mRNA interactions. We found that: i) the circRNA expression profile revealed 1,285 significant differences in circRNA expression, with circRNA expression downregulated in 594 samples and upregulated in 691 samples via interactions with miRNAs. The qRT-PCR validation experiments showed that hsa_circRNA_400071, hsa_circRNA_000543 and hsa_circRNA_001959 expression was consistent with the microarray analysis results. ii) 29,112 genes were found in the GC tissues and adjacent tissues, including 5,460 differentially expressed genes. Among them, 2,390 differentially expressed genes were upregulated and 3,070 genes were downregulated. Gene Ontology (GO) analysis of the differentially expressed genes revealed these genes involved in biological process classification, cellular component classification and molecular function classification. Pathway analysis of the differentially expressed genes identified 83 significantly enriched genes, including 28 upregulated genes and 55 downregulated genes. iii) 69 differentially expressed circRNAs were found that might adsorb specific miRNAs to regulate the expression of their target gene mRNAs. The conclusions are: i) differentially expressed circRNAs had corresponding miRNA binding sites. These circRNAs regulated the expression of target genes through interactions with miRNAs and might become new molecular biomarkers for GC in the future. ii) Differentially expressed genes may be involved in the occurrence of GC via a variety of mechanisms. iii) CD44, CXXC5, MYH9, MALAT1 and other genes may have important implications for the occurrence and development of GC through the regulation, interaction, and mutual influence of circRNA-miRNA-mRNA via different mechanisms.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Sui W,Shi Z,Xue W,Ou M,Zhu Y,Chen J,Lin H,Liu F,Dai Ydoi
10.3892/or.2017.5415subject
Has Abstractpub_date
2017-03-01 00:00:00pages
1804-1814issue
3eissn
1021-335Xissn
1791-2431journal_volume
37pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::To determine whether immunohistochemistry can identify BRCA1 mutations, immunohistochemical (IH) analysis was undertaken on paraffin sections of paired ovarian cancer and normal tissue using antibodies against both terminal regions of the BRCA1 protein. Ten patients at risk for BRCA1 mutations were studied. The result...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.7.5.949
更新日期:2000-09-01 00:00:00
abstract::KIAA1199 is a gene included in the Human Unidentified Gene-Encoded (HUGE) large protein database which contains more than 2,400 members identified in the Kazusa cDNA sequencing project. Early studies described KIAA1199 as an inner ear-specific protein in which 3 point mutations were found to be associated with non‑syn...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2014.3038
更新日期:2014-04-01 00:00:00
abstract::The aim of the present study was to investigate the efficacy of using human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) as gene delivery vectors in the treatment of ovarian cancer. Lentivectors overexpressing cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV-tk) (pGC-FU-CD-TK) ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2898
更新日期:2014-02-01 00:00:00
abstract::The tumour suppressor protein p53 (wild-type = wt-p53) is of major importance in the genetic integrity of the cell. Mutations of the p53-gene (mt-p53) are the most frequent genetic aberrations identified in different tumour entities. As analyzed in a wide variety of human malignomas, mt-p53 evokes a specific immune re...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000568
更新日期:2009-12-01 00:00:00
abstract::Oncolytic vaccinia virus is currently undergoing evaluation as a biological anticancer agent in clinical trials. This treatment exploits the lytic nature of a viral infection to eradicate the tumor mass in a cancer cell‑specific manner. So far, various vaccinia strains have been used as backbones in the design of onco...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6801
更新日期:2019-01-01 00:00:00
abstract::The morbidity and mortality of prostate cancer have been increasing recently, and the comprehensive treatment for prostate cancer is unable to achieve satisfactory outcomes. Quercetin is a natural flavonoid compound that has attracted increased interest and attention due to its anticancer activity. In vitro and in viv...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2015.3886
更新日期:2015-06-01 00:00:00
abstract::The precise mechanism through which the two estrogen receptor subtypes, ERα and ERβ, are linked to endometrial malignant progression is not fully understood. The aim of the present study was to examine their role in endometrial carcinoma cell migration, invasion and proliferation. We also explored the correlation betw...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2944
更新日期:2014-03-01 00:00:00
abstract::Urokinase plasminogen activator (uPA) and metalloproteinases (MMP) play key roles in invasion and metastasis, degrading extracellular matrix compounds and modulating tumor cell motility. Their regulation is an attractive therapeutic target for controlling tumor metastasis. Previously we have demonstrated that urokinas...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::The aims of this study were to investigate the expression levels of beta-catenin, Pin1 and cyclin D1 in salivary adenoid cystic carcinomas (SACC ) and to evaluate its clinical importance, furthermore, to elucidate whether beta-catenin expression was aberrant in SACC and whether Pin1 was involved in aberrant beta-caten...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract::MicroRNAs (miRNAs) play an important role in the genesis and development of gastric cancer. In the present study, we determined whether miRNA-135a-5p expression was increased in gastric cancer compared with adjacent non-tumor tissues using 20 pairs of gastric cancer and para-carcinoma tissue samples which were assesse...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3546
更新日期:2014-12-01 00:00:00
abstract::The purpose of this study was to investigate the radioprotective effect of HGFs (GM-CSF, IL-3 and SCF) in irradiated human peripheral blood mononuclear cells (PBMCs) in vitro, and the survival effect of lethally irradiated C3H mice in vivo. The irradiation of human PBMCs using a (137)Cs irradiator showed a dose-depend...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::Investigations into the cellular processing of radiolabeled monoclonal antibodies (mAbs) for their further use in radioimmunodiagnosis and cancer therapy are needed in order to understand the fate of internalized and catabolized mAbs. The anti-colorectal cancer mAb, A33, was labelled with 76Br and 125I using the direc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Curcumin, a naturally occurring yellow pigment isolated from turmeric, is a well known antioxidant with broad spectrum of anti-tumor activities against many human cancer cells. In this study, curcumin-induced cytotoxic effect of mouse-rat hybrid retina ganglion cells (N18) were investigated. For determining cell viabi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000411
更新日期:2009-07-01 00:00:00
abstract::It has been shown that overexpression of signal transducer and activator of transcription 3 (Stat3) contribute to the progression and metastasis of various solid tumors and that silencing Stat3 inhibits tumor growth in several types of cancer. Gene associated with retinoid-IFN-induced mortality 19 (GRIM-19), a Stat3-i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3233
更新日期:2014-08-01 00:00:00
abstract::A-kinase can inhibit RhoA activation through phosphorylating ser188 of RhoA. AKAP is a novel protein that can target PKA to different subcellular compartment. Evidence has been presented that PKA anchorage by AKAP is important for the kinase to exert its function. This study analyzed the role of PKA anchorage in PKA-i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::Breast cancer is one of the major health issues confronting women; however, treatment with conventional chemotherapeutic drugs is limited. Currently, paclitaxel is used as a therapeutic clinical agent to treat breast cancer that exerts antitumor activity in numerous types of cancer cell. Curcumin (diferuloylmethane), ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6603
更新日期:2018-10-01 00:00:00
abstract::MicroRNA-1 (miR-1) has been shown to function as a critical gene regulator in multiple types of cancers. However, the role of miR-1 in osteosarcoma has not been totally clarified. In the present study, we investigated the effects of miR-1 on osteosarcoma and the underlying mechanism. We found that miR-1 was downregula...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3274
更新日期:2014-09-01 00:00:00
abstract::c‑mesenchymal‑epithelial transition (Met) is a transmembrane tyrosine kinase receptor of hepatocyte growth factor (HGF). HGF/Met signaling stimulates numerous pathways, including the Ras/mitogenactivated protein kinase (MAPK), phosphatidylinositol 3‑kinase/protein kinase B and Wnt/β‑catenin pathways, which serve impor...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7799
更新日期:2020-12-01 00:00:00
abstract::The mammalian target of rapamycin (mTOR) is a key kinase acting downstream of growth factor receptor PI3K and AKT signaling, leading to processes resulting in increased cell size and proliferation through translation control. Rapamycin, a specific inhibitor of mTOR, results predomin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1855
更新日期:2012-09-01 00:00:00
abstract::Angiogenesis is crucial for the development and metastasis of human brain glioma. Based on our previous successful construction of a lentivirus-mediated alphastatin (an endogenous angiogenesis inhibitor) gene transfer system and our findings that alphastatin exhibited potent inhibit...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2187
更新日期:2013-03-01 00:00:00
abstract::Synovial sarcomas (SS) consistently show a characteristic chromosomal translocation, t(X;18)(p11;q11), which usually leads to the formation of 2 chimeric fusion transcripts, SYT-SSX1 and -SSX2. A recent multi-institutional retrospective study revealed that the SYT-SSX fusion type emerged as the only independent signif...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::Livistona chinensis seeds have been used for centuries to clinically treat various types of cancer. Our published data suggest that Livistona chinensis seeds are able to inhibit hepatocellular carcinoma (HCC) growth in vitro and in vivo via promotion of mitochondrial-dependent apoptosis. To further elucidate the molec...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3051
更新日期:2014-04-01 00:00:00
abstract::To estimate the incidence of Epstein-Barr virus-associated gastric carcinoma (EBV-GC) in Colombia and to clarify its clinicopathological features, we examined 178 consecutive gastric carcinoma cases, diagnosed during the period from 1996 to 1998, at Hospital Universitario del Valle in Cali, Colombia. The mean age of t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::Melanoma is the seventh most common malignancy in females and the fifth most common cancer in males worldwide. An increasing number of studies have reported that microRNA (miRNA) dysregulation is frequently observed in various types of human cancers, including melanoma. Abnormally expressed miRNAs play an important ro...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.6074
更新日期:2018-01-01 00:00:00
abstract::We aimed to investigate the relationship between miRNA-146a and latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC). The expression levels of LMP1 in 40 cases of NPC, 28 cases of chronic nasopharyngitis and NPC cell lines CNE1 and CNE1-GL (...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1933
更新日期:2012-10-01 00:00:00
abstract::Recent studies suggest that latexin (Lxn) expression is involved in stem cell regulation and that it plays significant roles in tumor cell migration and invasion. The clinicopathological significance of Lxn expression and its possible correlation with CD133 expression in pancreatic ductal adenocarcinoma (PDAC) is curr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4353
更新日期:2016-01-01 00:00:00
abstract::In the present study, a novel signaling pathway of microRNA-141 (miR-141)/fused in sarcoma (FUS) was investigated in neuroblastoma (NB). Gene expression of miR-141 was evaluated in 6 NB cell lines. IMR-32 and SH-SY5Y cells were transduced with the miR-141 mimic lentivirus. The effects of miR-141 upregulation on cell p...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4640
更新日期:2016-05-01 00:00:00
abstract::The present study aimed to investigate the effects of cold atmospheric plasma (CAP)‑activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7518
更新日期:2020-05-01 00:00:00
abstract::This study aimed to investigate the effect of RNA interference (RNAi)-mediated downregulation of the expression of the c-jun gene (a proto-oncogene) on the radiosensitivity of a radioresistant human nasopharyngeal carcinoma cell line (CNE-2R) and to validate its potential as an anticancer target. A lentiviral vector w...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3692
更新日期:2015-03-01 00:00:00
abstract::Gentian violet (GV) is a cationic triphenylmethane dye, with potent antifungal and antibacterial activity. We recently reported that in vitro GV suppresses the differentiation of bone resorbing osteoclasts while stimulating the differentiation and activity of bone forming osteoblasts. Breast cancer is highly metastati...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4190
更新日期:2015-10-01 00:00:00