当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Structure-activity relationship of a recombinant hybrid Manganese superoxide dismutase of Staphylococcus saprophyticus/S. equorum.

Structure-activity relationship of a recombinant hybrid Manganese superoxide dismutase of Staphylococcus saprophyticus/S. equorum.

Abstract：

:Recombinant hybrid Manganese superoxide dismutase from Staphyloccus saphropyticus/S. equorum (rMnSODSeq) exhibits stability at high temperatures. The enzyme occurs as a dimer that dissociates around 52°C prior to unfolding of the monomer around 64°C, demonstrating contribution of the dimeric form to stability. Here, structure - activity relationship of rMnSODSeq was evaluated on the basis of its activity and stability in the presence of inhibitors, NaCl, denaturants, detergents, reducing agents, and at different pH values. The activity was evaluated at both 37°C and 52°C, which the latter is the temperature for dissociation of the dimer. Dimer to monomer transition coincided with significant decrease in residual activity at 52°C. However, the activity assay results at 52°C and 37°C suggest spontaneous re-association of the monomer into dimer. Intriguingly, various new species with melting temperature (TM) values other than those of the dimer or monomer were observed. These species displayed medium to comparable level of residual activities to the native at 37°C. This report suggests that dimer to monomer transition may be not the only explanation for activity loss or decrease.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Retnoningrum DS,Arumsari S,Artarini A,Ismaya WT

doi

10.1016/j.ijbiomac.2017.01.096

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

222-227

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(16)31748-2

journal_volume

98

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Distribution of solvent and ligand molecules around aromatic side chains in proteins and its implication on carbonic anhydrase catalytic mechanism.

    abstract：:Interactions between aromatic phenylalanine, tyrosine and tryptophan residues and water as well as ligand molecules were analyzed by a computer search of a large number of high resolution structures in the protein data bank. Water molecules and oxygen ligands have a preference for the edges of the aromatic residues, b...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(95)01068-8

    authors： Håkansson K

    更新日期：1996-04-01 00:00:00

  • Common buckwheat-resistant starch as a suitable raw material for food production: A structural and physicochemical investigation.

    abstract：:Heat-moisture treatment (HMT) of starch is defined as a physical method to change its properties. Compared with maize and potato, starches from common buckwheat (Xinong9976 and Pingqiao2) were isolated and its morphology and physicochemical properties investigated by scanning electron microscope (SEM), X-ray diffracti...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.12.116

    authors： Gao L,Xia M,Li Z,Wang M,Wang P,Yang P,Gao X,Gao J

    更新日期：2020-02-15 00:00:00

  • pH-responsive poly(gellan gum-co-acrylamide-co-acrylic acid) hydrogel: Synthesis, and its application for organic dye removal.

    abstract：:A poly(gellan gum-co-acrylamide-co-acrylic acid) (GGDA) hydrogel was synthesized by modifying gellan gum (GG) with partly neutralized acrylic acid (AAc) and acrylamide (AAm) under the crosslinking agent trimethylolpropane triglycidyl ether. With the introduction of carboxyl and amino groups by the AAc and AAm, the GGD...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.03.024

    authors： Zheng M,Cai K,Chen M,Zhu Y,Zhang L,Zheng B

    更新日期：2020-06-15 00:00:00

  • Development of Ga-68 labeled, biotinylated thiosemicarbazone dextran-coated iron oxide nanoparticles as multimodal PET/MRI probe.

    abstract：:Bifunctional biotinylated thiosemicarbazone dextran-coated iron oxide Nanoparticles (NPs) were fabricated. Aldehyde groups of the oxidized dextran-coating layer were utilized to conjugate biotin as a tumor-targeting agent and thiosemicarbazide as a cation chelator on the surface of NPs. The final product was character...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.208

    authors： Gholipour N,Akhlaghi M,Mokhtari Kheirabadi A,Geramifar P,Beiki D

    更新日期：2020-04-01 00:00:00

  • Synthesis and characterization of iron oxide/cellulose nanocomposite film.

    abstract：:Iron oxide/cellulose high-performance nanocomposite film is successfully prepared by impregnation of iron oxide nanoparticles into a regenerated cellulose film. The structure, thermal stability and mechanical properties of the nanocomposite films are investigated by the wide-angle X-ray diffraction, Fourier transform ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.11.042

    authors： Yadav M,Mun S,Hyun J,Kim J

    更新日期：2015-03-01 00:00:00

  • Optimization of whey protein isolate-quince seed mucilage complex coacervation.

    abstract：:In this study, the complex coacervation of whey protein isolate (WPI) and quince seed mucilage (QSM) was studied as a function of pH (7.0-2.0), biopolymers concentration (0.05, 0.1 and 0.5%) and WPI:QSM ratio (10:90 to 90:10), according to protolytic titration, electrical conductivity (EC) and turbidity analyses. The ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.026

    authors： Ghadermazi R,Khosrowshahi Asl A,Tamjidi F

    更新日期：2019-06-15 00:00:00

  • Internal structure of high degree substitution acetylated potato starch by chemical surface gelatinization.

    abstract：:To understand the contribution of granule inner portion to the pasting property of acetylated potato starch under the electric field and ultrasonic treatment, these starches were subjected to chemical surface gelatinization by 4.0 mol/L CaCl2 to obtain remaining granules with pasting time 0, 6, 12, 18, 24, 30 min, res...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.12.102

    authors： Cao M,Gao Q

    更新日期：2020-02-15 00:00:00

  • Scutellaria baicalensis Georgi polysaccharide ameliorates DSS-induced ulcerative colitis by improving intestinal barrier function and modulating gut microbiota.

    abstract：:The aim of this study was to investigate the effect of a polysaccharide from Scutellaria baicalensis Georgi on UC. Gut microbiota dysbiosis is a worldwide problem associating with ulcerative colitis. One homogeneous polysaccharide, named SP2-1, was isolated from Scutellaria baicalensis Georgi. SP2-1 comprised mannose,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.10.259

    authors： Cui L,Guan X,Ding W,Luo Y,Wang W,Bu W,Song J,Tan X,Sun E,Ning Q,Liu G,Jia X,Feng L

    更新日期：2021-01-01 00:00:00

  • Protein phosphatase inhibitor-1 (PPI-1) has protective activities in stress conditions in E. coli.

    abstract：:Protein phosphatase inhibitor-1 (PPI-1) is a major inhibitor of protein phosphatase 1 (PP1), which regulates signal transduction in many eukaryotic cellular processes. Biophysical studies have shown that PPI-1 has a large Stokes radius and is heat stable, suggesting that it lacks extensive secondary structures. The un...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2006.01.001

    authors： Kim TD

    更新日期：2006-02-28 00:00:00

  • Chitosan-functionalized nanofibers: A comprehensive review on challenges and prospects for food applications.

    abstract：:Chitosan exhibits outstanding properties, which allow a wide range of applications. For this reason, chitosan-based biomaterials have been developed over the years and, among these biomaterials, chitosan-based nanomaterials may significantly change the material properties, which could result in some exceptional featur...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2018.11.042

    authors： de Farias BS,Sant'Anna Cadaval Junior TR,de Almeida Pinto LA

    更新日期：2019-02-15 00:00:00

  • TPR domain of Ser/Thr phosphatase of Aspergillus oryzae shows no auto-inhibitory effect on the dephosphorylation activity.

    abstract：:A Ser/Thr phosphatase gene cloned from Aspergillus oryzae, aoppt, revealed that the tetratricopeptide repeat (TPR) and catalytic domains of the full-length AoPPT are located at the N- and C-terminal regions, respectively, similar to those of human Ser/Thr phosphatase 5 (PP5) and yeast Ppt1. Four different regions of A...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2007.03.005

    authors： Feng B,Zhao CH,Tanaka S,Imanaka H,Imamura K,Nakanishi K

    更新日期：2007-08-01 00:00:00

  • Advances in upstream and downstream strategies of pectinase bioprocessing: A review.

    abstract：:This review aims to highlight the bioprocessing strategies behind one of the most commercially produced enzymes in the biotechnological market, pectinase. Significant steps in the upstream processing of this enzyme include its source, the selection of the substrate, the reaction conditions and the overall reactor desi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2020.06.224

    authors： John J,Kaimal KKS,Smith ML,Rahman PKSM,Chellam PV

    更新日期：2020-11-01 00:00:00

  • Optimized extraction of polysaccharides from Cymbopogon citratus and its biological activities.

    abstract：:In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.01.033

    authors： Thangam R,Suresh V,Kannan S

    更新日期：2014-04-01 00:00:00

  • A simple MWCNTs@paper biosensor for CA19-9 detection and its long-term preservation by vacuum freeze drying.

    abstract：:This paper introduces a cheap simple MWCNTs@paper biosensor for the detection of CA19-9, which is a biomarker of pancreatic cancer. By adding the CA19-9 antibody to the surface of MWCNTs which are deposited on the microporous filter paper, the correlation between the concentration of CA19-9 and resistance of biosensor...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.09.176

    authors： Jin W,Zhang R,Dong C,Jiang T,Tian Y,Yang Q,Yi W,Hou J

    更新日期：2020-02-01 00:00:00

  • Development of a new paper based nano-biosensor using the co-catalytic effect of tyrosinase from banana peel tissue (Musa Cavendish) and functionalized silica nanoparticles for voltammetric determination of l-tyrosine.

    abstract：:In this paper, a new and facile method for the electrochemical determination of l-tyrosine was designed. First, 3-mercaptopropyl trimethoxysilane-functionalized silica nanoparticles were added to a paper disc. Then, the banana peel tissue and the mediator potassium hexacyanoferrate were dropped onto the paper, respect...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.02.060

    authors： Rahimi-Mohseni M,Raoof JB,Ojani R,Aghajanzadeh TA,Bagheri Hashkavayi A

    更新日期：2018-07-01 00:00:00

  • Chitosan/Sterculia striata polysaccharides nanocomplex as a potential chloroquine drug release device.

    abstract：:Nanoparticles are produced by means of polyelectrolyte complexation (PEC) of oppositely charged polycationic chitosan (CH) with polyanionic polysaccharide extracted from Sterculia striata exudates (rhamnogalacturonoglycan (RG)-type polysaccharide). The nanoparticles formed with low-molar-mass CH are larger than those ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.03.070

    authors： Magalhães GA Jr,Moura Neto E,Sombra VG,Richter AR,Abreu CM,Feitosa JP,Paula HC,Goycoolea FM,de Paula RC

    更新日期：2016-07-01 00:00:00

  • Aerodynamic properties and in silico deposition of isoniazid loaded chitosan/thiolated chitosan and hyaluronic acid hybrid nanoplex DPIs as a potential TB treatment.

    abstract：:Existing therapies yield low drug encapsulation or accumulation in the lungs, hence the site-specific drug delivery remains the challenge for tuberculosis. Lately, dry powder inhalers (DPIs) are showing promising drug deposition in the deeper lung tissues. Biocompatible polymers with the ability to naturally recognize...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.10.192

    authors： Mukhtar M,Pallagi E,Csóka I,Benke E,Farkas Á,Zeeshan M,Burián K,Kókai D,Ambrus R

    更新日期：2020-12-15 00:00:00

  • Hypoglycemic activity in vitro of polysaccharides from Camellia oleifera Abel. seed cake.

    abstract：:We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro. The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjuga...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.04.054

    authors： Zhang S,Li X

    更新日期：2018-08-01 00:00:00

  • Rapid analysis of polyhydroxyalkanoate contents and its monomer compositions by pyrolysis-gas chromatography combined with mass spectrometry (Py-GC/MS).

    abstract：:Here, we report an analysis method for determining PHA (polyhydroxyalkanoates) contents and their monomer composition in microbial cells based on pyrolysis gas chromatography combined with mass spectrometry (Py-GC/MS). Various kinds of microbial cells accumulating different PHA contents and monomer compositions were p...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2021.01.108

    authors： Khang TU,Kim MJ,In Yoo J,Sohn YJ,Jeon SG,Park SJ,Na JG

    更新日期：2021-01-21 00:00:00

  • TRIP13 is a predictor for poor prognosis and regulates cell proliferation, migration and invasion in prostate cancer.

    abstract：:Thyroid hormone receptor interactor 13 (TRIP13) has been reported to be overexpressed in serval types of human cancers, and regulate tumor cell proliferation, migration and invasion. However, the role of TRIP13 in prostate cancer was still unclear. In our study, the correlation between TRIP13 expression and clinical p...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.168

    authors： Dong L,Ding H,Li Y,Xue D,Li Z,Liu Y,Zhang T,Zhou J,Wang P

    更新日期：2019-01-01 00:00:00

  • Isolation and analysis of a novel acidic polysaccharide from the case of squid pen.

    abstract：:A new non-sulphated acidic polysaccharide with an average molecular mass of 55 kDa was isolated from squid pen case after papain digestion and beta-elimination. This polysaccharide contains mainly L-iduronic acid, D-glucuronic acid, D-galactosamine, D-glucosamine and significant amounts of neutral sugars as glucose, g...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(99)00066-5

    authors： Theocharis AD,Karamanos NK,Tsegenidis T

    更新日期：1999-10-01 00:00:00

  • Fabrication of pure chitosan nanofibrous membranes as effective absorbent for dye removal.

    abstract：:The pure chitosan nanofibrous membranes with average fiber diameter of 86±18, 114±17,164±28nm were successfully prepared by electrospinning. Batch adsorption experiments of using chitosan nanofibrous membranes as adsorbent to remove acid blue-113 were conducted. The adsorption capacity of 1377mg/g was achieved by the ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.08.072

    authors： Li C,Lou T,Yan X,Long YZ,Cui G,Wang X

    更新日期：2018-01-01 00:00:00

  • Efficient adsorption of methylene blue and lead ions in aqueous solutions by 5-sulfosalicylic acid modified lignin.

    abstract：:As a kind of biomass that exists widely in plants, lignin shows much diversity as a functional material. In order to improve the adsorption ability, lignin was chemically modified by 5-sulfosalicylic acid and then used to adsorb methylene blue (MB) and Pb2+ from aqueous solutions. The results showed that the 5-sulfosa...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.213

    authors： Jin Y,Zeng C,Lü QF,Yu Y

    更新日期：2019-02-15 00:00:00

  • Oxidative stress level and dehydrin gene expression pattern differentiate two contrasting cucumber F1 hybrids under high fertigation treatment.

    abstract：:Two cucumber F1 cultivar hybrids were investigated for stress tolerance markers upon application of different strength of Hoagland fertigation solutions (HG). 'Joker' and 'Oitol' cultivar hybrids were studied, representing typically field grown and greenhouse cultivated genotypes, respectively. At standard fertigation...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.050

    authors： Oszlányi R,Mirmazloum I,Pónya Z,Szegő A,Jamal S,Bat-Erdene O,Papp I

    更新日期：2020-10-15 00:00:00

  • Expression and characterization of a novel recombinant cytotoxin II from Naja naja oxiana venom: A potential treatment for breast cancer.

    abstract：:Breast cancer (BC) is among the leading causes of mortality from cancer in women. Many of the available anticancer drugs have various side effects. Therefore, researchers are seeking novel anticancer agents particularly from natural compounds and in this regard, snake venom is still one of the main sources of drug dis...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.130

    authors： Derakhshani A,Silvestris N,Hajiasgharzadeh K,Mahmoudzadeh S,Fereidouni M,Paradiso AV,Brunetti O,Atarod D,Safarpour H,Baradaran B

    更新日期：2020-11-01 00:00:00

  • The exquisite structural biophysics of the Golgi Reassembly and Stacking Proteins.

    abstract：:Golgi Reassembly and Stacking Proteins (GRASPs) were firstly described as crucial elements in determining the structure of the Golgi complex. However, data have been accumulating over the years showing GRASPs can participate in various cell processes beyond the Golgi maintenance, including cell adhesion and migration,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2020.08.203

    authors： Mendes LFS,Fontana NA,Reddy ST,Uversky VN,Costa-Filho AJ

    更新日期：2020-12-01 00:00:00

  • In vivo antioxidant and anti-inflammatory effects of soluble dietary fiber Konjac glucomannan in type-2 diabetic rats.

    abstract：:Although the anti-diabetic properties of Konjac glucomannan (KGM) have been reported previously; however, the molecular pathways of its anti-diabetic properties are not clear. The present study hypothesized that KGM could mitigate oxidative stress and inflammation. Three doses of KGM (40, 80, 120 mg/kg b.w.) decreased...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.105

    authors： Zhao Y,Jayachandran M,Xu B

    更新日期：2020-09-15 00:00:00

  • Structure characterization of one polysaccharide from Lepidium meyenii Walp., and its antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells.

    abstract：:The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp. were investigated. The molecular weight was estimated to be 4.67 × 105 Da by HPLC-ELSD analysis. Monosaccharide composition was deter...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.06.117

    authors： Wang W,Zhang F,Li Q,Chen H,Zhang W,Yu P,Zhao T,Mao G,Feng W,Yang L,Wu X

    更新日期：2018-10-15 00:00:00

  • Synthesis of a chitosan/polyvinyl alcohol/activate carbon biocomposite for removal of hexavalent chromium from aqueous solution.

    abstract：:In this study, a biocomposite of chitosan/poly vinyl alcohol/activated carbon was synthesized and used for hexavalent chrome removal from aqueous solution. The synthesized adsorbent was characterized by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FTIR) analysis. The effect of important variables...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.044

    authors： Nowruzi R,Heydari M,Javanbakht V

    更新日期：2020-03-15 00:00:00

  • Scrutinizing the different pectin types on stability of an Iranian traditional drink "Doogh".

    abstract：:Doogh is a fermented dairy drink which is highly consumed by Iranian people. Stability of this healthy drink was investigated in terms of sedimentation rate, viscosity, density and particle size characteristics including surface-weighted mean diameter (D32), Span and particle uniformity. Eight treatments were performe...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.06.034

    authors： Joudaki H,Mousavi M,Safari M,Razavi SH,Emam-Djomeh Z,Gharibzahedi SM

    更新日期：2013-09-01 00:00:00