Abstract:
:Rabbit apolipoprotein A-I (apo A-I) of molecular weight 27,612 contained 241 amino acids. In contrast to its human counterpart which has 3 methionine residues, the rabbit protein possesses only one and therefore produces 2 fragments after CNBr cleavage (CNBr I and II, NH2- and COOH-terminal, respectively). From a series of monoclonal antibodies raised against human apo A-I, 2 (A05 and A16) cross-reacted with rabbit apo A-I. In the present study, we show that A05 recognizes the rabbit CNBr I fragment while the integrity of the intermediate region between the 2 CNBr fragments (including the methionine residue) is required for the expression of the A16 antigenic determinant. Competition experiments were performed between human 125I-labelled high density lipoprotein (HDL) and a variety of preparations of human and rabbit apo A-I (including the purified and delipidated protein, complexes of dimyristoylphosphatidylcholine (DMPC) containing apo A-I, HDL subfractions and whole serum). The A05 antigenic determinant was expressed identically in all these fractions of both species. In contrast the A16 showed poor reactivity with delipidated apo A-I, the apparent affinity constant being about 100 times less than for HDL. These data suggest that phospholipids improve the recognition of apo A-I by the A16 antibody. The similar immunoreactivity of the human and rabbit proteins in the present study is consistent with the view that the NH2-terminal region contains the major portion activating lecithin:cholesterol acyltransferase.
journal_name
Atherosclerosisjournal_title
Atherosclerosisauthors
Leroy A,Vu-Dac N,Theret N,Pio F,Fruchart JCdoi
10.1016/0021-9150(89)90028-2subject
Has Abstractpub_date
1989-09-01 00:00:00pages
9-19issue
1eissn
0021-9150issn
1879-1484pii
0021-9150(89)90028-2journal_volume
79pub_type
杂志文章相关文献
ATHEROSCLEROSIS文献大全abstract:BACKGROUND AND AIMS:South Asians are at increased risk for cardiovascular disease (CVD). Aortic valve calcium (AVC) is associated with CVD risk and aortic stenosis. Elevated Lp(a) is a heritable risk factor for CVD and AVC. AVC prevalence and its association with Lp(a) have not been studied in South Asians. METHODS:Am...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2020.09.010
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:Leukocyte count is recognized as an inflammatory marker and a predictor of cardiovascular events. However, it is uncertain whether the contribution of leukocyte count to the risk of cardiovascular disease is independent of smoking. METHODS:The subjects were 4492 male employees aged 40-59 years who worked fo...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2006.09.002
更新日期:2007-11-01 00:00:00
abstract::We studied the cytotoxic effect of copper-oxidized LDL in human primary human umbilical vein endothelial cells (HUVEC) and the immortalized EA.hy 926 cell line. Copper oxidized LDL (50-200 microg apoB/ml) induced concentration-dependent apoptotic cell death in HUVEC but did not induce apoptosis in EA.hy 926 cells. Onl...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(99)00170-7
更新日期:1999-11-01 00:00:00
abstract::We previously reported linkage for plasma levels of high-density lipoprotein cholesterol (HDL-C) on 15q21 in Caucasian families from the National Heart, Lung, and Blood Institute Family Heart Study (NHLBI FHS). Hepatic lipase gene (LIPC), which has a major role in lipoprotein metabolism, resides within the linkage reg...
journal_title:Atherosclerosis
pub_type: 杂志文章,多中心研究
doi:10.1016/j.atherosclerosis.2008.09.007
更新日期:2009-05-01 00:00:00
abstract:INTRODUCTION:The possible cause of accelerated atherosclerosis in NAFLD may be the relationship with the MetS and its components. Our primary goal was to evaluate the relationship between NAFLD and subclinical atherosclerosis in adult male patients between 20 and 40 years of age. Moreover, we aimed to investigate the c...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2015.04.009
更新日期:2015-06-01 00:00:00
abstract::Over 200 risk factors for cardiovascular disease (CVD) have now been identified. Among these, the three most important are (1) abnormal lipids, including the fact that there are more than 15 types of cholesterol-containing lipoproteins and four different types of triglyceride-rich particles, some of which are very ath...
journal_title:Atherosclerosis
pub_type: 杂志文章,评审
doi:10.1016/0021-9150(96)05851-0
更新日期:1996-07-01 00:00:00
abstract::Numerous epidemiological studies have shown that lipoprotein(a) (Lp(a)) is an independent risk factor for the premature development of cardiovascular disease. In spite of such evidence, the structural and functional features of this atherogenic, cholesterol-rich particle are not clearly understood. We have demonstrate...
journal_title:Atherosclerosis
pub_type: 杂志文章,评审
doi:10.1016/s0021-9150(97)00111-1
更新日期:1997-08-01 00:00:00
abstract::Whether an association, causative or not, exists between the level of serum selenium and the risk of ischaemic heart disease (IHD) remains unsettled. We investigated the issue in a cohort of 3387 males aged 53-74 years (mean 63). Based on information about health status, life-style and socioeconomic factors given in a...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(92)90035-f
更新日期:1992-09-01 00:00:00
abstract::Endothelial cell lipotoxicity mediated by accumulation of free fatty acids is an early event in the pathogenesis of atherosclerosis. The energy-sensor AMP-activated protein kinase (AMPK) is a key regulator of endothelial cell lipid metabolism. To test the hypothesis that bradykinin (BK) regulates AMPK and fatty acid o...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2007.12.003
更新日期:2008-09-01 00:00:00
abstract::Macrophage scavenger receptors class A (MSR) are thought to play an important role in atherogenesis by mediating the unrestricted uptake of modified lipoproteins by macrophages in the vessel wall leading to foam cell formation. To investigate the in vivo role of the MSR in this process, a transgenic mouse model expres...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(99)00204-x
更新日期:1999-12-01 00:00:00
abstract:UNLABELLED:Recent studies have shown, that chronic flavonoids treatment improves vascular function and cardiovascular remodeling by decreasing superoxide anion production as well as by increasing NO realize from endothelial cells. A progressive decrease in systolic blood pressure and reduction of low-density lipoprotei...
journal_title:Atherosclerosis
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.atherosclerosis.2006.12.032
更新日期:2007-10-01 00:00:00
abstract:RATIONALE:Lipoprotein lipase (LPL) X447 homozygotes are characterized by enhanced conversion of TRL apoB100. Here, we set out to investigate whether this LPL variant is also associated with enhanced apoB48 clearance. Therefore, we evaluated apoB48 kinetics in X447 homozygotes in the fed state by infusion of isotope L-[...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2006.08.038
更新日期:2007-10-01 00:00:00
abstract:UNLABELLED:Transglutaminases play an important role in vascular remodeling, calcification, cell adhesion and endothelial barrier function. In this study we investigate the influence of combined inhibition of both tissue-type transglutaminase (TG2) and the plasma transglutaminase FXIIIA on early lesion development. MET...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2010.07.054
更新日期:2010-11-01 00:00:00
abstract:INTRODUCTION:Fenofibrate has shown to reduce major cardiovascular events and slow angiographic progression of coronary atherosclerosis. The postulated mechanism of action is via the activation of peroxisomal proliferator-activated receptor-alpha (PPAR-alpha), a nuclear transcription factor that controls a variety of ce...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2006.02.036
更新日期:2007-01-01 00:00:00
abstract::The insertion/deletion (I/D) polymorphism of the ACE gene may be involved in structural arterial changes. Aim of the present study was to assess the relationship between the ACE I/D gene and vessel wall stiffness among older adults. The study was conducted within the Rotterdam study, a population-based cohort study in...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2004.01.012
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:Detection of inflamed atherosclerotic plaques is of crucial importance. The carotid artery cuff-model in ApoE(-/-) mice results in shear-stress induced atherosclerosis with inflamed plaques upstream (US) and 'stable' plaques downstream (DS) of the cuff. We evaluated the potential of F-18-FDG PET/CT to differ...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2014.03.008
更新日期:2014-06-01 00:00:00
abstract::Expression of ATP binding cassette transporter A1 (ABCA1), a major regulator of high density lipoprotein (HDL) biogenesis, is known to be up-regulated by the transcription factor liver X receptor (LXR) alpha, and expression is further enhanced by activation of the peroxisome proliferator activated receptors (PPARs). W...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2009.01.008
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVES:Urinary cystatin C (u-CysC) is a new biomarker for acute tubular kidney dysfunction and may also indicate chronic tubular dysfunction. Chronic kidney disease is an important cardiovascular risk factor, however it is not known if u-CysC is a risk marker for cardiovascular death. METHODS:The association betwe...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2014.02.020
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:To determine whether endogenous sex hormones (estradiol (E2), testosterone (T), sex hormone binding globulin (SHBG), and follicle stimulating hormone (FSH)) are longitudinally associated with progression of atherosclerosis among women at midlife. METHODS:249 Pre- or early peri-menopausal women (42-57 years) ...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2012.07.025
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVES:Markers of non-specific inflammation, such as C-reactive protein (CRP) or leukocyte count are increased in end-stage renal disease patients. Recent studies have shown positive associations between inflammatory markers and cardiovascular mortality in kidney transplant recipients, but these analyses had been l...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2010.01.004
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND AND AIMS:Studying atherosclerotic calcification in vivo requires mouse models with genetic modifications. Previous studies showed that injection of recombinant adeno-associated virus vector (AAV) encoding a gain-of-function mutant PCSK9 into mice promotes atherosclerosis. We aimed to study cardiovascular cal...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2016.06.011
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVE:Paraoxonase 1 (PON1) is a high-density lipoprotein (HDL)-associated enzyme which possess anti-atherogenic properties. Our aim was to analyze the effect of HDL phospholipids on HDL-associated paraoxonase (PON1) catalytic and biological activities. METHODS AND RESULTS:In HDL isolated from di-oleoyl-phosphatidy...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2008.05.016
更新日期:2009-02-01 00:00:00
abstract::Oxidized LDL (OxLDL) is a proatherogenic lipoprotein, accumulating in the vascular wall and contributing to the pathogenesis of vascular dysfunction early in the development of atherosclerosis. Enhanced serum levels of OxLDL, as well as antibodies against its epitopes, are predictive for endothelial dysfunction and co...
journal_title:Atherosclerosis
pub_type: 杂志文章,评审
doi:10.1016/j.atherosclerosis.2005.10.005
更新日期:2006-04-01 00:00:00
abstract::Protein synthesis in smooth muscle cells from normal and atherosclerotic rabbit aortas was studied. Cultures of cells from the third passage were incubated with [3H]proline and the synthesis rates of collagen and total protein measured from the radioactivities incorporated into protein-bound hydroxyproline and proline...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/0021-9150(80)90089-1
更新日期:1980-09-01 00:00:00
abstract:RATIONALE:Macrophage cholesterol homeostasis maintenance is the result of a balance between influx, endogenous synthesis, esterification/hydrolysis and efflux. Excessive accumulation of cholesterol leads to foam cell formation, which is the major pathology of atherosclerosis. Previous studies have shown that miR-27 (mi...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2014.02.008
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:Lipid rafts are cholesterol-enriched microdomains on cell membranes. We hypothesized that these microdomains could involve modified low-density lipoprotein (LDL) uptake. METHODS AND RESULTS:Co-localizations of cholesterol-enriched microdomains and CD204 during the uptake of acetyl LDL (AcLDL) and oxidized LD...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2010.10.019
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND AND AIMS:A recently identified locus for coronary artery disease (CAD) tagged by rs8042271 is in a region of tight linkage disequilibrium (LD) between 2 genes (MFGE8, ABHD2) previously linked to atherosclerosis. Here we have explored the regulatory framework of this region to identify its functional relation...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2019.02.012
更新日期:2019-05-01 00:00:00
abstract::Inflammation is thought to play a central role in the etiology and outcome of atherosclerosis. Animal studies as well as in vitro and in vivo human studies suggest that host factors modulate the magnitude and extent of inflammatory responses. We investigated familial aggregation of three systemic markers of inflammati...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/s0021-9150(00)00586-4
更新日期:2001-02-15 00:00:00
abstract:BACKGROUND AND AIMS:Peripheral artery disease (PAD) is associated with poor mobility and fatigue, but the relationship between preclinical ankle-brachial index (ABI) and early markers of fatigue and functional decline has not been defined. METHODS:570 adults, 50 and older, from the Baltimore Longitudinal Study of Agin...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2018.03.037
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND AND AIMS:The recently identified adiponectin paralogue C1q and tumor necrosis factor-related protein 1 (CTRP1) has been associated with obesity-linked disorders and coronary atherosclerosis. So far, the impact of circulating CTRP1 on the incidence of future cardiovascular events is unclear. Therefore, we aim...
journal_title:Atherosclerosis
pub_type: 杂志文章
doi:10.1016/j.atherosclerosis.2019.04.222
更新日期:2019-07-01 00:00:00