Abstract:
:Sulforaphane (SFN) is a naturally occurring chemopreventive agent, which effectively inhibits proliferation of HepG2 human hepatocellular carcinoma cells via mitochondria‑mediated apoptosis. Endoplasmic reticulum stress is considered the most important cause of cell apoptosis; therefore, the present study aimed to determine whether the endoplasmic reticulum pathway was involved in SFN-induced apoptosis of HepG2 cells. An MTT assay was used to detect the inhibitory effects of SFN on HepG2 cells. Fluorescence microscopy was used to observe the morphological changes in apoptotic cells, and western blot analysis was conducted to detect the expression of binding immunoglobulin protein (Bip)/glucose-regulated protein 78 (GRP78), X‑box binding protein‑1 (XBP‑1) and BH3 interacting domain death agonist (Bid). Furthermore, flow cytometry was used to determine the apoptotic rate of HepG2 cells, and the protein expression of C/EBP homologous protein (CHOP)/growth arrest‑ and DNA damage‑inducible gene 153 (GADD153) and caspase-12 in HepG2 cells. The results indicated that SFN significantly inhibited the proliferation of HepG2 cells; the half maximal inhibitory concentration values were 32.03±0.96, 20.90±1.96 and 13.87±0.44 µmol/l, following treatment with SFN for 24, 48 and 72 h, respectively. Following 48 h of SFN treatment (10, 20 and 40 µmol/l), the apoptotic rates of HepG2 cells were 31.8, 61.3 and 77.1%, respectively. Furthermore, after 48 h of exposure to SFN, the cells presented typical morphological alterations of apoptosis, as detected under fluorescence microscopy. Treatment with SFN for 48 h also significantly upregulated the protein expression levels of Bip/GRP78, XBP‑1, caspase‑12, CHOP/GADD153 and Bid in HepG2 cells. In conclusion, endoplasmic reticulum stress may be considered the most important mechanism underlying SFN-induced apoptosis in HepG2 cells.
journal_name
Mol Med Repjournal_title
Molecular medicine reportsauthors
Zou X,Qu Z,Fang Y,Shi X,Ji Ydoi
10.3892/mmr.2016.6016subject
Has Abstractpub_date
2017-01-01 00:00:00pages
331-338issue
1eissn
1791-2997issn
1791-3004journal_volume
15pub_type
杂志文章abstract::Genistein is an important chemopreventive agent against atherosclerosis and cancer. However, whether genistein is effective in the treatment of lung cancer, and its underlying mechanism, remains to be determined. The present study demonstrated that genistein treatment of A549 lung cancer cells decreased viability in a...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.6260
更新日期:2017-04-01 00:00:00
abstract::FW523-3, a new lipopeptide compound, was recently isolated and purified from the culture broth of a marine Micromonospora chalcea. FW523-3 was shown to inhibit the proliferation of certain cancer cells. However, the spectra and the underlying mechanism of its antitumor activity are unclear. In this study, the MTT and ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2011.496
更新日期:2011-07-01 00:00:00
abstract::microRNAs (miRNAs) are small non‑coding RNAs that regulate gene expression by binding to the untranslated regions (UTRs) of target mRNAs. Bioinformatic software predicted that MYCN, a gene overexpressed in aggressive neuroblastoma cells, is a target gene of miRNA‑202 (miR‑202) and that the promoter region of miR‑202 c...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2013.1845
更新日期:2014-02-01 00:00:00
abstract::Chronic administration of D‑galactose (D‑gal) is a useful method for establishing a model of natural aging in the auditory system. Previous studies have demonstrated that NADPH oxidases (NOXs) may be an important source of reactive oxygen species (ROS) in the peripheral auditory system (PAS) and cause an increase in m...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2014.2653
更新日期:2014-12-01 00:00:00
abstract::1,2-Dimethylthdrazine (DMH) has been known to induce vascular neoplasms, such as malignant endothelioma, in animal experiments through the induction of abnormal proliferation of human umbilical vein endothelial cells (HUVECs). We studied the effect of protein kinase C (PKC) isoforms...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2012.932
更新日期:2012-08-01 00:00:00
abstract::Diabetic nephropathy (DN) is a serious complication of diabetes and can cause an increased mortality risk. It was previously reported that NLR family pyrin domain containing 3 (NLRP3) inflammasome is involved in the pathogenesis of diabetes. However, the underlying mechanism is not clearly understood. In the present s...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9164
更新日期:2018-08-01 00:00:00
abstract::Senescence is a result of cellular stress and is a potential mechanism for regulating cancer. As a member of the mitogen‑activated protein kinase family, ERK1/2 (extracellular signal‑regulated protein kinase) has an important role in delivering extracellular signals to the nucleus, and these signals regulate the cell ...
journal_title:Molecular medicine reports
pub_type: 杂志文章,评审
doi:10.3892/mmr.2018.9712
更新日期:2019-02-01 00:00:00
abstract::In recent years, accumulating evidence has demonstrated that microRNAs (miRs, miRNAs) may serve an important role in the occurrence and development of tumors. miR‑23a‑5p has been confirmed as an oncogene in numerous diseases through gene chip analysis. However, as the most common type of renal tumor, the expression an...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.6829
更新日期:2017-08-01 00:00:00
abstract::Dietary n‑3 polyunsaturated fatty acid (PUFA) exerts anti‑inflammatory and immunoregulatory effects via downregulation of the innate and adaptive immune responses. However, the effect of dietary n‑3 PUFA on experimental Crohn's Disease (CD) in rats and the underlying mechanisms are unclear. The present study aimed to ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7673
更新日期:2017-12-01 00:00:00
abstract::Aldosterone has an important role in the progression of renal fibrosis. In the present study, the concentration of aldosterone and klotho (KL) in the serum of patients with chronic kidney disease (CKD) were analyzed. A negative correlation was observed between aldosterone and KL, suggesting that KL may serve a protect...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9781
更新日期:2019-03-01 00:00:00
abstract::The problems of aging, obesity and a number of types of metabolic syndromes, including diabetes, are associated with a higher risk of non-alcoholic fatty liver disease (NAFLD). However, the mechanisms of occurrence and development of NAFLD in humans remain unclear. Sterol regulatory...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2012.953
更新日期:2012-09-01 00:00:00
abstract::The hepatitis C virus (HCV) alternate reading frame protein (ARFP or F protein) of the HCV 1b genotype is a double-frameshift product of the HCV core protein (Core). The discovery of HCV F protein challenges various biological functions attributed to Core. However, the specific characteristics of the host cellular imm...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2013.1816
更新日期:2014-02-01 00:00:00
abstract::Nasopharyngeal carcinoma (NPC) is endemic in the southern provinces of China and Southeast Asia. It has been reported that microRNA‑122 (miR‑122) and tripartite motif‑containing protein 29 (TRIM29) serve important roles in many types of tumor. The present study aimed to evaluate the expression of miR‑122 and TRIM29, a...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.8894
更新日期:2018-06-01 00:00:00
abstract::Gliomas are the most common type of primary brain tumor. Using current standard treatment regimens, the prognosis of patients with gliomas remains poor, which is predominantly due to the resistance of glioma cells to chemotherapy. The organ microenvironment has been implicated in the pathogenesis and survival of tumor...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4680
更新日期:2016-02-01 00:00:00
abstract::The oral fluoropyrimidine S-1 has marked efficacy in treating metastatic colorectal cancer patients. In the present study, we explored the therapeutic potential of combined in vivo treatment in the human colon cancer cell lines COL-1, KM12C and KM20C with consecutive oral S-1 and leucovorin (LV) in 2-week therapeutic ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr_00000111
更新日期:2009-05-01 00:00:00
abstract::Thyroid stimulating hormone (TSH) consists of an α‑subunit and a unique β‑subunit. The first in‑frame TSHβ splice variant produced by the cells of immune system was identified in 2009. The TSHβ splice variant and native TSHβ exhibit different expression profiles, and research has been conducted to elucidate the role o...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.10082
更新日期:2019-05-01 00:00:00
abstract::We wish to retract our article entitled 'Clinical, pathological and genetic characteristics of autosomal dominant inherited dynamin 2 centronuclear myopathy' published in Molecular Medicine Reports 13: 4273-4278, 2016. The article was submitted by the first author, Xinhong Liu, without the prior knowledge of the corre...
journal_title:Molecular medicine reports
pub_type: 杂志文章,撤回出版物
doi:10.3892/mmr.2016.5267
更新日期:2016-07-01 00:00:00
abstract::Genetically modified mesenchymal stem cells have been used in attempts to increase the expression of interleukin‑1 receptor antagonist (IL‑1Ra); however, the attempts thus far have been unsuccessful. The aim of the present study was to investigate whether the lentivirus transduced IL‑1Ra gene was able to be stably exp...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.4003
更新日期:2015-09-01 00:00:00
abstract::Non‑alcoholic steatohepatitis (NASH) is a common clinicopathological condition. Currently, the pathogenesis of NASH remains unknown, and no optimal therapy option currently exists. It has previously been demonstrated that diallyl disulfide (DADS) was capable of attenuating liver dysfunction, as DADS supplementation ha...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.10316
更新日期:2019-08-01 00:00:00
abstract::Paraquat (PQ) poisoning‑induced pulmonary fibrosis is one of the primary causes of mortality in patients with PQ poisoning. The potential mechanism of PQ‑induced pulmonary fibrosis was thought to be mediated by inflammation. Recently, bone marrow‑derived mesenchymal stem cells (BMSCs) have been considered as a potenti...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.9976
更新日期:2019-04-01 00:00:00
abstract::The contractile to synthetic phenotypic switching of vascular smooth muscle cells (VSMCs) in response to fibroblast growth factor (FGF) has been previously described. However, the role of the inflammatory response induced by FGF signaling in VSMCs and its occurrence in atherosclerosis remains unclear. In the present s...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2019.10249
更新日期:2019-07-01 00:00:00
abstract::The present study aimed to investigate the functional effects of microRNA‑195 on ovarian cancer cells and the underling mechanism involved. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression of microRNA‑195 in patients with ovarian cancer. Cell proliferation and apoptosis w...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2018.9098
更新日期:2018-08-01 00:00:00
abstract::Resistin, an adipocytokine secreted by fat tissues, has been shown to be associated with increased local and systemic complications in acute pancreatitis (AP). However, the mechanism underlying the effect of resistin in the aggravation of AP remains to be elucidated. The aim of the present study was to investigate the...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.6027
更新日期:2017-01-01 00:00:00
abstract::In the present study, the effect of glucosamine administration (1.5 or 3 g/day) on cartilage and bone metabolism was investigated in bicycle racers, using cartilage‑ and bone‑specific biomarkers, including C‑terminal cross‑linked telopeptides of type II collagen (CTX‑II), C‑terminal...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2013.1289
更新日期:2013-03-01 00:00:00
abstract::Several studies have noted benign thecoma‑fibroma tumors with positive F‑18 fluorodeoxyglucose (FDG) accumulation mimicking malignant ovarian tumors following F‑18 FDG positron emission tomography (PET). The present study analyzed four cases with false‑positive F‑18 FDG PET/computed tomography (CT) diagnoses of thecom...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.5016
更新日期:2016-05-01 00:00:00
abstract::Lysyl oxidase-like 2 (LOXL2) is key in the hepatocellular carcinoma (HCC) tumor microenvironment and metastatic niche formation. However, its effect on proliferation and clinical parameters in HCC require further elucidation. The present study aimed to investigate LOXL2 expression in HCC from in vitro and clinical asp...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.5474
更新日期:2016-09-01 00:00:00
abstract::Cerebral infarction is a cerebrovascular disease caused by local brain ischemic necrosis or softening, which is associated with diabetes, obesity, hypertension and rheumatic heart arrhythmia. Previous studies have indicated that aspirin is a potential oral anticoagulant in the treatment of cerebral ischemic stroke. Ho...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2017.7879
更新日期:2018-01-01 00:00:00
abstract::The aim of the present study was to investigate Fas expression in tumor samples from patients with gastric cancer, in order to determine the involvement of the Fas signaling pathway. The protein expression levels of Fas, caspase-8, caspase‑3 and poly (adenosine diphosphate‑ribose) polymerase 1 (PARP1) were examined in...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2016.6037
更新日期:2017-02-01 00:00:00
abstract::Ras‑GTPase‑activating protein SH3 domain‑binding protein 1 (G3BP1) has been reported to be of importance in the occurrence and development of colon cancer. However, the underlying mechanisms remain largely unknown. Therefore, the aim of the present study was to investigate the role of Wnt/β‑catenin signaling in G3BP1‑...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2020.11527
更新日期:2020-11-01 00:00:00
abstract::Cardiac stem cells (CSCs) can differentiate into cardiac muscle‑like cells; however, it remains unknown whether CSCs may possess the ability to differentiate into pacemaker cells. The aim of the present study was to determine whether angiotensin II (Ang II) could promote the specialization of CSCs into pacemaker‑like ...
journal_title:Molecular medicine reports
pub_type: 杂志文章
doi:10.3892/mmr.2015.3149
更新日期:2015-05-01 00:00:00