miRNA‑195 suppresses cell proliferation of ovarian cancer cell by regulating VEGFR2 and AKT signaling pathways.

Abstract:

:The present study aimed to investigate the functional effects of microRNA‑195 on ovarian cancer cells and the underling mechanism involved. Reverse transcription‑quantitative polymerase chain reaction was used to measure the expression of microRNA‑195 in patients with ovarian cancer. Cell proliferation and apoptosis were measured with MTT assay and flow cytometry, respectively. Caspase‑3/9 activity, vascular endothelial growth factor receptor (VEGFR)2 and phosphorylated protein kinase B (p‑AKT) protein expression were analyzed using caspase‑3/9 activity kits and western blot analysis. The expression of microRNA‑195 was downregulated in ovarian cancer, compared with the normal control group. Furthermore, microRNA‑195 suppresses cell proliferation and induced apoptosis of ovarian cancer cells. In addition, microRNA‑195 suppressed VEGFR2 and p‑AKT protein expression in ovarian cancer cells. The inhibition of VEGFR2 and p‑AKT increased the functional effects of microRNA‑195 on apoptosis of ovarian cancer cells. The results demonstrated that microRNA‑195 suppresses cell proliferation of ovarian cancer cells through regulation of VEGFR2 and AKT signaling pathways.

journal_name

Mol Med Rep

authors

Chen J

doi

10.3892/mmr.2018.9098

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

1666-1673

issue

2

eissn

1791-2997

issn

1791-3004

journal_volume

18

pub_type

杂志文章
  • RUNX2 controls human IPO8 basal transcription in Saos-2 cells.

    abstract::Runt-related transcription factor 2 (RUNX2) is a vital regulatory factor that controls osteoblast-specific gene expression; however, RUNX2‑regulated genes in human mesenchymal stem cells (hMSCs) remain to be fully elucidated. In the present study, chromatin immunoprecipitation (ChIP)-on-chip analysis of RUNX2 in hMSCs...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5356

    authors: Xiong J,Hu Z,Wang T,Xu X,Liu J,Wu P,Che X,Li W

    更新日期:2016-08-01 00:00:00

  • Correlation between the overexpression of urokinase receptor isoform uPAR (D1D2) and hepatic cell malignant transformation.

    abstract::In order to provide an abundant source of specimen and to reveal the correlation between the overexpression of urokinase plasminogen activator receptor isoform uPAR (D1D2) and hepatic cell malignant transformation, the optimal liver cell culture method was selected from three cell culture methods to culture and separa...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2006

    authors: Zhou Y,Lǚ X,Li S,Zhan L

    更新日期:2014-05-01 00:00:00

  • Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface.

    abstract::Mineralized bone matrix constituted with collagenous and non-collagenous proteins was synthesized by osteoblasts differentiated from mesenchymal stem cells. Secretory leukocyte protease inhibitor (SLPI), a serine protease inhibitor, promotes cell migration and proliferation, and suppresses the inflammatory response. R...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5381

    authors: Choi BD,Lee SY,Jeong SJ,Lim DS,Cha HJ,Chung WG,Jeong MJ

    更新日期:2016-08-01 00:00:00

  • Lentivirus‑mediated knockdown of MeCP2 inhibits the growth of colorectal cancer cells in vitro.

    abstract::Methyl‑CpG‑binding protein 2 (MeCP2) is a transcriptional repressor that has been implicated in tumor onset and progression. Compared with normal and other tumorous tissue, MeCP2 is highly expressed in well‑differentiated adenocarcinoma and mucinous adenocarcinoma tissues, particularly at the invasion site of colorect...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4612

    authors: Song N,Li K,Wang Y,Chen Z,Shi L

    更新日期:2016-01-01 00:00:00

  • Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells.

    abstract::Mechanical stress produced by orthodontic forces is a factor in the remodeling of periodontal ligaments (PDLs) and alveolar bone. It has been reported that the expression of a number of cytokines associated with osteoclastogenesis is upregulated when compressive forces act on osteoblasts and PDL cells. The present stu...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4141

    authors: Hayakawa T,Yoshimura Y,Kikuiri T,Matsuno M,Hasegawa T,Fukushima K,Shibata K,Deyama Y,Suzuki K,Iida J

    更新日期:2015-10-01 00:00:00

  • RBEL1 is required for osteosarcoma cell proliferation via inhibiting retinoblastoma 1.

    abstract::Osteosarcoma is the most common type of primary malignant tumor of the bone. However, mechanisms underlying osteosarcoma cell proliferation are poorly understood. The present study shows that RBEL1, a newly identified Rab-like GTPase, may be a key regulator of osteosarcoma cell proliferation. Knockdown of RBEL1 in ost...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4670

    authors: Tang H,Ji F,Sun J,Xie Y,Xu Y,Yue H

    更新日期:2016-02-01 00:00:00

  • Comprehensive analysis of expression profiles of long non‑coding RNAs with associated ceRNA network involved in gastric cancer progression.

    abstract::Long non‑coding RNAs (lncRNAs) play critical roles in the development and progression of cancers. The present study aimed to identify novel lncRNAs and associated microRNAs (miRNAs or miRs) and mRNAs in gastric cancer. Differentially expressed lncRNAs (DElncRNAs) and differentially expressed mRNAs (DEmRNAs) of 6 paire...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10478

    authors: Gu W,Ren JH,Zheng X,Hu XY,Hu MJ

    更新日期:2019-09-01 00:00:00

  • Presence of multimeric isoforms of human C-reactive protein in tissues and blood.

    abstract::The baseline concentration of C-reactive protein (CRP) has been associated with a wide array of human diseases. In epidemiological studies and in the clinic, CRP is typically measured as a pentamer, composed of 5 identical CRP subunits. The present study aimed to determine whether other isoforms were present in the bl...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5922

    authors: Li Q,Xu W,Xue X,Wang Q,Han L,Li W,Lv S,Liu D,Richards J,Shen Z,Ma L,Song Q

    更新日期:2016-12-01 00:00:00

  • Aberrant miRNA expression response to UV irradiation in human liver cancer cells.

    abstract::microRNAs (miRNAs) are small, highly conserved, non-coding RNAs that regulate gene expression at the post‑transcriptional level. The expression of these small RNA genes is tightly regulated during development, differentiation and apoptosis of normal cells, however, they are often deregulated in various types of cancer...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.1901

    authors: Liang G,Li G,Wang Y,Lei W,Xiao Z

    更新日期:2014-03-01 00:00:00

  • Hair growth is promoted by BeauTop via expression of EGF and FGF‑7.

    abstract::Minoxidil and finasteride have been approved to treat hair loss by the Food and Drug Administration. However, the further elucidation of treatments for hair loss, including those using Chinese herbal medicine, remains important clinically. BeauTop (BT) is a health food supplement which contains Ginseng radix, Astragal...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8917

    authors: Lee CY,Yang CY,Lin CC,Yu MC,Sheu SJ,Kuan YH

    更新日期:2018-06-01 00:00:00

  • Calcineurin/NFAT signaling pathway mediates titanium particle‑induced inflammation and osteoclast formation by inhibiting RANKL and M‑CSF in vitro.

    abstract::Wear particles serve a central role in periprosthetic osteolysis, which leads to the aseptic loosening of prostheses. In the present study a lentiviral vector was constructed to silence macrophage colony stimulating factor (M‑CSF) and receptor activator of nuclear factor κ‑B ligand (RANKL) genes, which synergistically...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7670

    authors: Zhang Y,Jiang P,Li W,Liu X,Lu Y,Huang Z,Song K

    更新日期:2017-12-01 00:00:00

  • (‑)‑Epigallocatechin‑3‑gallate induces apoptosis in human pancreatic cancer cells via PTEN.

    abstract::Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a cancer suppressor gene and an important negative regulator in the phosphatidylinositide 3‑kinase (PI3K)/protein kinase B (Akt)/ mechanistic target of rapamycin (mTOR) signaling pathway. The PI3K/Akt/mTOR pathway can promote cancer cell survival, proli...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5277

    authors: Liu S,Xu ZL,Sun L,Liu Y,Li CC,Li HM,Zhang W,Li CJ,Qin W

    更新日期:2016-07-01 00:00:00

  • Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins.

    abstract::The aim of the present study was to analyze the protein composition of ductal breast carcinoma and the surrounding normal tissue in individual patients using comparative 2D proteomics and mass spectrometry to detect candidate disease biomarkers for diagnosis and prognosis. Samples of normal and cancerous tissue obtain...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1375

    authors: Carcoforo P,Ura B,Mischiati C,Squerzanti M,Lanzara V,Cervellati C,Calza R,De Laureto PP,Frare E,Portinari M,Feriotto G,Lanzara S,Agostinelli E,Bergamini CM

    更新日期:2013-05-01 00:00:00

  • Protective effect of rhein against oxidative stress-related endothelial cell injury.

    abstract::Endothelial cell injury caused by reactive oxygen species (ROS) plays a critical role in the pathogenesis of atherosclerosis. Therefore, phytochemicals or antioxidants that inhibit the production of ROS have clinical value for the treatment of atherosclerosis. Rhein is one of the most important active components of rh...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.793

    authors: Zhong XF,Huang GD,Luo T,Deng ZY,Hu JN

    更新日期:2012-05-01 00:00:00

  • TET1 partially mediates HDAC inhibitor-induced suppression of breast cancer invasion.

    abstract::Histone deacetylases (HDACs) are important in chromatin remodeling and epigenetic regulation of gene expression. Histone deacetylase inhibitors (HDACi) have highly effective anti-metastatic and anti-angiogenic activity in various types of cancer, while the molecular mechanisms involved in this process are not fully un...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2517

    authors: Lu HG,Zhan W,Yan L,Qin RY,Yan YP,Yang ZJ,Liu GC,Li GQ,Wang HF,Li XL,Li Z,Gao L,Chen GQ

    更新日期:2014-11-01 00:00:00

  • Acetylshikonin suppresses invasion of Porphyromonas gingivalis‑infected YD10B oral cancer cells by modulating the interleukin-8/matrix metalloproteinase axis.

    abstract::The development of pharmaceutical agents possessing anti‑invasive and anti‑metastatic abilities, as well as apoptotic activity, is important in decreasing the incidence and recurrence of oral cancer. Cancer cells are known to acquire invasiveness not only through epigenetic changes, but also from inflammatory stimuli ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8151

    authors: Cho BH,Jung YH,Kim DJ,Woo BH,Jung JE,Lee JH,Choi YW,Park HR

    更新日期:2018-02-01 00:00:00

  • STRA6 as a possible candidate gene for pathogenesis of osteoporosis from RNA‑seq analysis of human mesenchymal stem cells.

    abstract::To identify novel candidate genes associated with osteoporosis, RNA‑sequence analysis of human mesenchymal stem cells (hMSCs) from patients with osteoporosis (G3) and osteopenia (G2), and healthy controls (G1) was performed. Differentially expressed genes (DEGs) from among the three groups were identified. DEGs were s...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7072

    authors: Song I,Choi YJ,Jin Y,Kim JW,Koh JT,Ji HM,Jeong SY,Won YY,Kim W,Chung YS

    更新日期:2017-10-01 00:00:00

  • miR‑124‑3p regulates angiogenesis in peripheral arterial disease by targeting STAT3.

    abstract::Peripheral arterial disease (PAD) is the third leading cause of cardiovascular morbidity worldwide, after coronary artery disease and stroke. As endogenous regulators of gene expression, microRNAs (miRs) are implicated in the development and progression of various diseases, including types of cancer, autoimmune diseas...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11538

    authors: Shi Y,Xu X,Luan P,Kou W,Li M,Yu Q,Zhuang J,Xu Y,Peng W,Jian W

    更新日期:2020-12-01 00:00:00

  • Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis.

    abstract::Lumbar disc disease (LDD) is common in aged populations, and it is primarily caused by intervertebral disc degeneration (IDD). Cartilage intermediate layer protein (CILP), which is specifically expressed in intervertebral discs (IVDs), is suspected to be associated with IDD. However, it remains unclear whether CILP co...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8588

    authors: He J,Feng C,Sun J,Lu K,Chu T,Zhou Y,Pan Y

    更新日期:2018-04-01 00:00:00

  • De novo transcriptome analysis and gene expression profiling of fish scales isolated from Carassius auratus during space flight: Impact of melatonin on gene expression in response to space radiation.

    abstract::Astronauts are inevitably exposed to two major risks during space flight, microgravity and radiation. Exposure to microgravity has been discovered to lead to rapid and vigorous bone loss due to elevated osteoclastic activity. In addition, long‑term exposure to low‑dose‑rate space radiation was identified to promote DN...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11363

    authors: Furusawa Y,Yamamoto T,Hattori A,Suzuki N,Hirayama J,Sekiguchi T,Tabuchi Y

    更新日期:2020-10-01 00:00:00

  • β‑Lapachone ameliorates L‑DOPA‑induced dyskinesia in a 6‑OHDA‑induced mouse model of Parkinson's disease.

    abstract::The dopamine precursor 3,4‑dihydroxyphenyl‑ l‑alanine (L‑DOPA) is the most widely used symptomatic treatment for Parkinson's disease (PD); however, its prolonged use is associated with L‑DOPA‑induced dyskinesia in more than half of patients after 10 years of treatment. The present study investigated whether co‑treatme...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2021.11856

    authors: Ryu YK,Park HY,Go J,Lee IB,Choi YK,Lee CH,Kim KS

    更新日期:2021-03-01 00:00:00

  • Ephrin type‑A receptor 2 regulates sensitivity to paclitaxel in nasopharyngeal carcinoma via the phosphoinositide 3‑kinase/Akt signalling pathway.

    abstract::Ephrin type‑A receptor 2 (EphA2) is a receptor tyrosine kinase that is associated with cancer cell metastasis. There has been little investigation into its impact on the regulation of sensitivity to paclitaxel in nasopharyngeal carcinoma (NPC). In the present study, upregulation of EphA2 expression enhanced the surviv...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2799

    authors: Wang Y,Liu Y,Li G,Su Z,Ren S,Tan P,Zhang X,Qiu Y,Tian Y

    更新日期:2015-02-01 00:00:00

  • MYBL2 protects against H9c2 injury induced by hypoxia via AKT and NF‑κB pathways.

    abstract::Cardiovascular diseases have become one of the major public health problems in many countries. The downregulation of MYBL2 was found in H9c2 and native cardiomyocytes cells after hypoxia treatment. The present study aimed to investigate the effects of MYB proto‑oncogene like 2 (MYBL2) on H9c2 injury induced by hypoxia...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8387

    authors: Shao M,Ren Z,Zhang R

    更新日期:2018-03-01 00:00:00

  • MicroRNA‑29a enhances cisplatin sensitivity in non‑small cell lung cancer through the regulation of REV3L.

    abstract::Cisplatin‑based chemotherapy may greatly enhance patient prognosis; however, chemotherapy resistance remains an obstacle to curing patients with non‑small cell lung cancer (NSCLC). The aim of the present study was to explore the microRNAs (miRs) that could regulate cisplatin sensitivity and provide a potential treatme...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9723

    authors: Chen X,Zhu H,Ye W,Cui Y,Chen M

    更新日期:2019-02-01 00:00:00

  • Matrine inhibits the migratory and invasive properties of nasopharyngeal carcinoma cells.

    abstract::Matrine is a widely used Chinese herbal medicine that has historically been used in the treatment of inflammation and cancer. However, the antimetastatic effects and associated molecular mechanisms of matrine on nasopharyngeal carcinoma (NPC) remain to be elucidated. Therefore, the aims of the present study were to as...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3276

    authors: Sun B,Xu M

    更新日期:2015-06-01 00:00:00

  • Bioinformatic analysis of microRNA expression in Parkinson's disease.

    abstract::Parkinson's disease (PD) is a type of movement disorder caused by loss of dopamine‑producing neurons in the midbrain. In order to identify the synergistic microRNA (miRNA) pattern in PD, miRNA and mRNA double expression profiles of PD were downloaded. Differentially expressed miRNA and mRNA were identified [P<0.01, fo...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2837

    authors: Hao B,Chen X,Dai D,Zou C,Wu X,Chen J

    更新日期:2015-02-01 00:00:00

  • β-Elemene inhibits proliferation through crosstalk between glia maturation factor β and extracellular signal‑regulated kinase 1/2 and impairs drug resistance to temozolomide in glioblastoma cells.

    abstract::β-elemene, a plant-derived drug extracted from Curcuma wenyujin, has demonstrated marked antiproliferative effects on glioblastoma, while toxicity remains low. However, the underlying molecular mechanisms of the antitumor activity of β-elemene remain to be elucidated. Previously, it was identified that the glia matura...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2273

    authors: Zhu TZ,Li XM,Luo LH,Xu YH,Cao P,Liu Y,Liang GB

    更新日期:2014-08-01 00:00:00

  • Upregulation of MAPK10, TUBB2B and RASL11B may contribute to the development of neuroblastoma.

    abstract::The present study aimed to investigate genes and transcription factors (TFs) that may contribute to neuroblastoma (NB) development. The GSE78061 dataset that included 25 human NB cell lines and four retinal pigment epithelial cell lines was used to analyze differentially expressed genes (DEGs) between groups. Function...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10589

    authors: Liu J,Li Y

    更新日期:2019-10-01 00:00:00

  • Effect of fractionated irradiation on the expression of multidrug resistance genes in the CNE1 human nasopharyngeal carcinoma cell line.

    abstract::Nasopharyngeal carcinoma (NPC) often develops drug resistance following radiotherapy. The molecular basis of radiotherapy-related multidrug resistance (MDR) remains unclear. In the present study, we investigated the effect of fractionated irradiation on the expression of the MDR-1 gene and the MDR-associated protein P...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1148

    authors: Ji XN,Yang F,Sui XM,Wang FG,Ge RG,Quan XL,Zhao T,Gao BW,Wang RY

    更新日期:2013-01-01 00:00:00

  • [Corrigendum] Effects of the Tangningtongluo formula as an alternative strategy for diabetics via upregulation of insulin receptor substrate-1.

    abstract::Subsequently to the publication of this article, the authors have realized that Fig. 4 contained some incorrectly incorporated data panels; specifically, the A‑d, B‑b and B‑c panels did not display the correct data. The revised version of Fig. 4 featuring the correct data for the A‑d, B‑b and B‑c panels is shown on th...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,已发布勘误

    doi:10.3892/mmr.2019.10649

    authors: Cheng L,Song J,Li G,Liu Y,Wang Y,Meng X,Sun G,Sun X

    更新日期:2019-11-01 00:00:00