Abstract:
:In 2010, a pathogenic flavivirus termed duck Tembusu virus (DTMUV) caused widespread outbreak of egg-drop syndrome in domesticated ducks in China. Although the glycoprotein E of DTMUV is an important structural component of the virus, the B-cell epitopes of this protein remains uncharacterized. Using phage display and mutagenesis, we identified a minimal B-cell epitope, 374EXE/DPPFG380, that mediates binding to a nonneutralizing monoclonal antibody. DTMUV-positive duck serum reacted with the epitope, and amino acid substitutions revealed the specific amino acids that are essential for antibody binding. Dot-blot assays of various flavivirus-positive sera indicated that EXE/DPPFG is a cross-reactive epitope in most flaviviruses, including Zika, West Nile, Yellow fever, dengue, and Japanese encephalitis viruses. These findings indicate that the epitope sequence is conserved among many strains of mosquito-borne flavivirus. Protein structure modeling revealed that the epitope is located in domain III of the DTMUV E protein. Together, these results provide new insights on the broad cross-reactivity of a B-cell binding site of the E protein of flaviviruses, which can be exploited as a diagnostic or therapeutic target for identifying, studying, or treating DTMUV and other flavivirus infections.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Li C,Bai X,Meng R,Shaozhou W,Zhang Q,Hua R,Liu JH,Liu M,Zhang Ydoi
10.1038/srep36288subject
Has Abstractpub_date
2016-11-08 00:00:00pages
36288issn
2045-2322pii
srep36288journal_volume
6pub_type
杂志文章abstract::A new, efficient method based on a series of matrices is introduced to completely describe the detailed topology of individual domains and their topology evolution in three-dimensional cellular structures. With this approach, we found a lot of new topological grain forms which are never reported before, i.e., there ar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25877
更新日期:2016-05-10 00:00:00
abstract::To compare the performance of eight score systems (MELD, uMELD, MELD-Na. iMELD, UKELD, MELD-AS, CTP, and mCTP) in predicting the post-transplant mortality, we analyzed the data of 6,014 adult cirrhotic patients who underwent liver transplantation between January 2003 and December 2010 from the China Liver Transplant R...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42253
更新日期:2017-02-13 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-69680-x
更新日期:2020-08-27 00:00:00
abstract::The peptidoglycan of Staphylococcus aureus is highly amidated. Amidation of α-D-isoglutamic acid in position 2 of the stem peptide plays a decisive role in the polymerization of cell wall building blocks. S. aureus mutants with a reduced degree of amidation are less viable and show increased susceptibility to methicil...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31098-x
更新日期:2018-08-28 00:00:00
abstract::Pro-inflammatory and amyloidogenic S100A9 protein is an important contributor to Alzheimer's disease (AD) pathology. Traumatic brain injury (TBI) is viewed as a precursor state for AD. Here we have shown that S100A9-driven amyloid-neuroinflammatory cascade was initiated in TBI and may serve as a mechanistic link betwe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31141-x
更新日期:2018-08-27 00:00:00
abstract::Hepatitis C virus (HCV) has long been observed to take advantage of the host mitochondria to support viral replication and assembly. The HCV core protein has been implicated to fragment host mitochondria. In this report, we have discovered that the non-structural protein 5A (NS5A) plays an instructive role in attachin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23464
更新日期:2016-03-24 00:00:00
abstract::Brain-computer interface (BCI) systems having the ability to classify brain waves with greater accuracy are highly desirable. To this end, a number of techniques have been proposed aiming to be able to classify brain waves with high accuracy. However, the ability to classify brain waves and its implementation in real-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45605-1
更新日期:2019-06-24 00:00:00
abstract::The ability to convert photons of different wavelengths directly into mechanical motion is of significant interest in many energy conversion and reconfigurable technologies. Here, using few layer 2H-MoS2 nanosheets, layer by layer process of nanocomposite fabrication, and strain engineering, we demonstrate a reversibl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34831
更新日期:2016-10-07 00:00:00
abstract::Studies focusing on biofilm assembly in deep-sea environments are rarely conducted. To examine the effects of substrate type on microbial community assembly, biofilms were developed on different substrates for different durations at two locations in the Red Sea: in a brine pool and in nearby bottom water (NBW) adjacen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06647
更新日期:2014-10-17 00:00:00
abstract::Biological research requires wide-field optical imaging techniques with resolution down to the nanometer scale to study the biological process in a sub-cell or single molecular level. To meet this requirement, wide-field structured illumination method (WFSIM) has been extensively studied. The resolution of WFSIM is de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01521-w
更新日期:2017-05-02 00:00:00
abstract::Ultrasonic spray coating process (USCP) with high material -utilization, low manufacture costs and compatibility to streamline production has been attractive in researches on photoelectric devices. However, surface tension exists in the solvent is still a huge obstacle to realize smooth organic film for organic light ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37042
更新日期:2016-11-22 00:00:00
abstract::Nile tilapia (Oreochromis niloticus) is a globally significant aquaculture species rapidly gaining status as a farmed commodity. In West Africa, wild Nile tilapia genetic resources are abundant yet knowledge of fine-scale population structure and patterns of natural genetic variation are limited. Coinciding with this ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53295-y
更新日期:2019-11-14 00:00:00
abstract::Japan has often been viewed as an Asian country that possesses a genetically homogenous community. The basis for partitioning the country into prefectures has largely been geographical, although cultural and linguistic differences still exist between some of the districts/prefectures, especially between Okinawa and th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17855
更新日期:2015-12-09 00:00:00
abstract::Intrinsically disordered proteins are abundant in the eukaryotic proteome, and they are implicated in a range of different diseases. However, there is a paucity of experimental data on molecular details of the coupled binding and folding of such proteins. Two interacting and relatively well studied disordered protein ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02076
更新日期:2013-01-01 00:00:00
abstract::Anticarsia gemmatalis (velvetbean caterpillar) and Chrysodeixis includens (soybean looper) are two important defoliation pests of soybeans. In the present study, we have investigated the susceptibility and brush border membrane-binding properties of both species to Bacillus thuringiensis Cry1Ea toxin. Bioassays perfor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54850-3
更新日期:2019-12-03 00:00:00
abstract::Typhoid fever poses significant burden on healthcare systems in Southeast Asia and other endemic countries. Several epidemiological and genomic studies have attributed pseudogenisation to be the major driving force for the evolution of Salmonella Typhi although its real potential remains elusive. In the present study,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07457
更新日期:2014-12-12 00:00:00
abstract::The subjective experience of thermal pain follows the detection and encoding of noxious stimuli by primary afferent neurons called nociceptors. However, nociceptor morphology has been hard to access and the mechanisms of signal transduction remain unresolved. In order to understand how heat transducers in nociceptors ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17670
更新日期:2015-12-07 00:00:00
abstract::The objective of this study was to investigate the influence of the genes encoding the KIR receptors and their HLA ligands in the susceptibility of ocular toxoplasmosis. A total of 297 patients serologically-diagnosed with toxoplasmosis were selected and stratified according to the presence (n = 148) or absence (n = 1...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep36632
更新日期:2016-11-09 00:00:00
abstract::Anthracnose caused by Colletotrichum is one of the most severe diseases of Hevea brasiliensis. However, research on the diversity and geographical distribution of Colletotrichum remains limited in China. In this study, we investigated the phylogenetic diversity of Colletotrichum isolates associated with symptomatic ti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28166-7
更新日期:2018-07-11 00:00:00
abstract::Honeybee pupae morphology can be affected by a number of stressor, but in vivo investigation is difficult. A computed tomography (CT) technique was applied to visualize a comb's inner structure without damaging the brood. The CT scan was performed on a brood comb containing pupae developed from eggs laid by the queen ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46474-4
更新日期:2019-07-23 00:00:00
abstract::The purpose of this study was to determine the significance of deep structural lesions for impairment of consciousness following hemorrhagic stroke and recovery at ICU discharge. Our study focused on deep lesions that previously were implicated in studies of disorders of consciousness. We analyzed MRI measures obtaine...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41042-2
更新日期:2019-03-12 00:00:00
abstract::The sweetpotato whitefly Bemisia tabaci (Gennadius) was challenged with different combinations of matrine (insecticide) and Lecanicillium muscarium (entomopathogenic fungus). Our results revealed a synergistic relationship between matrine and L. muscarium on mortality and enzyme activities of B. tabaci. To illustrate ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46558
更新日期:2017-04-20 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) is a devastating cause of diarrhea in pigs worldwide. Most of studies have focused on molecular and pathogenic characterization of PEDV, whereas there were limited studies in understanding the role of gut microbiota (GM) in viral-associated diarrhea. Here, using the Illumina MiSe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17830-z
更新日期:2017-12-12 00:00:00
abstract::The gene separation anxiety (san) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of this enzyme is still poorly understood. Naa50/San was originally proposed to be required for centromeric sis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39118
更新日期:2016-12-20 00:00:00
abstract::Most organisms are able to maintain systemic water homeostasis over a wide range of external or dietary osmolarities. The excretory system, composed of the kidneys in mammals and the Malpighian tubules and hindgut in insects, can increase water conservation and absorption to maintain systemic water homeostasis, which ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07993
更新日期:2015-01-23 00:00:00
abstract::We introduce a non-invasive approach for optogenetic regulation in biological cells through highly scattering skull tissue using wavefront shaping. The wavefront of the incident light was systematically controlled using a spatial light modulator in order to overcome multiple light-scattering in a mouse skull layer and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13289
更新日期:2015-08-21 00:00:00
abstract::Recently, detecting biological particles by analyzing their mechanical properties has attracted increasing attention. To detect and identify different bioparticles and estimate their dimensions, a mechanical nanosensor is introduced in this paper. To attract particles, numerous parts of the substrate are coated with d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60296-9
更新日期:2020-02-25 00:00:00
abstract::Controlled surface wettability for oil has been intensively studied to remove industrial oil waste or oil spill pollution from seas or rivers. In particular, external stimuli-induced special wetting materials, such as photo-responsive TiO2, have attracted considerable attention for oil-water separation. In this study,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12908
更新日期:2015-08-11 00:00:00
abstract::As the world's oceans are currently threatened by anthropogenic pollution and climate change, coral breeding has become an important conservation method, since it can limit marine organisms' exposure to sub-optimal environment conditions. However, the aquarium environment is inherently different from the ocean, and th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33341-x
更新日期:2018-10-11 00:00:00
abstract::Amorphous silicon oxycarbide (SiOC) is of great technological interest. However, its atomic-level structure is not well understood. Using density functional theory calculations, we show that the clustering tendency of C atoms in SiOC is extremely sensitive to hydrogen (H): without H, the C-C interaction is attractive,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13051
更新日期:2015-08-13 00:00:00