Abstract:
:Serine is the only amino acid that is encoded by two disjoint codon sets so that a tandem substitution of two nucleotides is required to switch between the two sets. Previously published evidence suggests that, for the most evolutionarily conserved serines, the codon set switch occurs by simultaneous substitution of two nucleotides. Here we report a genome-wide reconstruction of the evolution of serine codons in triplets of closely related species from diverse prokaryotes and eukaryotes. The results indicate that the great majority of codon set switches proceed by two consecutive nucleotide substitutions, via a threonine or cysteine intermediate, and are driven by selection. These findings imply a strong pressure of purifying selection in protein evolution, which in the case of serine codon set switches occurs via an initial deleterious substitution quickly followed by a second, compensatory substitution. The result is frequent reversal of amino acid replacements and, at short evolutionary distances, pervasive homoplasy.
journal_name
Proc Natl Acad Sci U S Aauthors
Rogozin IB,Belinky F,Pavlenko V,Shabalina SA,Kristensen DM,Koonin EVdoi
10.1073/pnas.1615832113subject
Has Abstractpub_date
2016-11-15 00:00:00pages
13109-13113issue
46eissn
0027-8424issn
1091-6490pii
1615832113journal_volume
113pub_type
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