Involvement of outside DNA sequences in the major kinetic path by which EcoRI endonuclease locates and leaves its recognition sequence.

abstract：:The initiator RNAs of mammalian Okazaki fragments are thought to be removed by RNase HI and the 5'-3' flap endonuclease (FEN1). Earlier evidence indicated that the cleavage site of RNase HI is 5' of the last ribonucleotide at the RNA-DNA junction on an Okazaki substrate. In current work, highly purified calf RNase HI ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murante RS,Henricksen LA,Bambara RA

更新日期：1998-03-03 00:00:00

abstract：:DNA from one biopsy sample of invasive cancer of the cervix contained sequences hybridizing with human papillomavirus (HPV) type 11 DNA only under nonstringent conditions. This DNA was molecularly cloned in lambda phage. Under stringent conditions of hybridization it cross-hybridized to a minor extent (less than 0.1%)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dürst M,Gissmann L,Ikenberg H,zur Hausen H

更新日期：1983-06-01 00:00:00

abstract：:Oxidative stress exacerbates neovascularization (NV) in many disease processes. In this study we investigated the mechanism of that effect. Mice deficient in superoxide dismutase 1 (Sod1(-/-) mice) have increased oxidative stress and show severe ocular NV that is reduced to baseline by antioxidants. Compared with wild...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong A,Shen J,Zeng M,Campochiaro PA

更新日期：2011-08-30 00:00:00

abstract：:The goldfish (Carassius auratus) visual pathway displays continuous growth and plasticity throughout life. Since homeobox genes are important transcriptional regulators in development, we searched for homeobox genes in the adult goldfish retina and brain. Using the PCR, we discovered a repertoire of homeobox sequences...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine EM,Schechter N

更新日期：1993-04-01 00:00:00

abstract：:Cooperation via production of common goods is found in diverse life forms ranging from viruses to social animals. However, natural selection predicts a "tragedy of the commons": Cheaters, benefiting from without producing costly common goods, are more fit than cooperators and should destroy cooperation. In an attempt ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waite AJ,Shou W

更新日期：2012-11-20 00:00:00

abstract：:Newborns are at increased risk of jaundice, a condition in which excess bilirubin accumulates in blood. Left untreated, jaundice can lead to neurological impairment and death. Jaundice resulting from unconjugated hyperbilirubinemia is easily treated with exposure to blue light, and phototherapy systems have been devel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keahey PA,Simeral ML,Schroder KJ,Bond MM,Mtenthaonnga PJ,Miros RH,Dube Q,Richards-Kortum RR

更新日期：2017-12-19 00:00:00

abstract：:Eukaryotic cells require mechanisms to establish the proportion of cellular volume devoted to particular organelles. These mechanisms are poorly understood. From a screen for plastid-to-nucleus signaling mutants in Arabidopsis thaliana, we cloned a mutant allele of a gene that encodes a protein of unknown function tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Larkin RM,Stefano G,Ruckle ME,Stavoe AK,Sinkler CA,Brandizzi F,Malmstrom CM,Osteryoung KW

更新日期：2016-02-23 00:00:00

abstract：:Gene regulation is a process with many steps allowing for stochastic biochemical reactions, which leads to expression noise-i.e., the cell-to-cell stochastic fluctuation in protein abundance. Such expression noise can give rise to drastically diverse phenotypes, even within isogenic cell populations. Although numerous...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li J,Min R,Vizeacoumar FJ,Jin K,Xin X,Zhang Z

更新日期：2010-06-08 00:00:00

abstract：:Squalene and sterol carrier protein of liver plays a general role as a vehicle for cholesterol and its water-insoluble precursors; the carrier protein is essential for enzymic cholesterol synthesis. Liver microsomal enzymes contain a small amount of endogenous carrier protein, which is readily removed by washing or pu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ritter MC,Dempsey ME

更新日期：1973-01-01 00:00:00

abstract：:A three-dimensional model of the protein arrangement in the Escherichia coli 30S ribosome was constructed by using computerized multidimensional scaling of immunoelectron microscope data. This enabled data comparison between the new electron microscope technique and other methods such as crosslinking, chemical protect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaffney PT,Craven G

更新日期：1978-07-01 00:00:00

abstract：:This paper reports a general in situ method to grow a polymer conjugate solely from the C terminus of a recombinant protein. GFP was fused at its C terminus with an intein; cleavage of the intein provided a unique thioester moiety at the C terminus of GFP that was used to install an atom transfer radical polymerizatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao W,Liu W,Christensen T,Zalutsky MR,Chilkoti A

更新日期：2010-09-21 00:00:00

abstract：:Infection is a major complication of implantable medical devices, which provide a scaffold for biofilm formation, thereby reducing susceptibility to antibiotics and complicating treatment. Hematogenous implant-related infections following bacteremia are particularly problematic because they can occur at any time in a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Cheng LI,Helfer DR,Ashbaugh AG,Miller RJ,Tzomides AJ,Thompson JM,Ortines RV,Tsai AS,Liu H,Dillen CA,Archer NK,Cohen TS,Tkaczyk C,Stover CK,Sellman BR,Miller LS

更新日期：2017-06-27 00:00:00

abstract：:Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson LH,Mankovitz R,Baker RM,Till JE,Siminovitch L,Whitmore GF

更新日期：1970-06-01 00:00:00

abstract：:The basolateral membranes of Aplysia californica foregut absorptive cells contain both Cl(-)-stimulated ATPase and ATP-dependent Cl- transport activities, and each was inhibited by orthovanadate. Both of these orthovanadate-sensitive activities were reconstituted into proteoliposomes. The reaction sequence kinetics we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerencser GA,Zelezna B

更新日期：1993-09-01 00:00:00

abstract：:Many animals employ sex pheromones to find mating partners during their reproductive seasons. However, most sex pheromones of vertebrates remain to be identified. Over the past 20 years, steroids and prostaglandins have been identified as sex pheromones in several fishes. These pheromones are broadly termed "hormonal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yambe H,Kitamura S,Kamio M,Yamada M,Matsunaga S,Fusetani N,Yamazaki F

更新日期：2006-10-17 00:00:00

abstract：:Defining the molecules that regulate tumor cell survival is an essential prerequisite for the development of targeted approaches to cancer treatment. Whereas many studies aimed at identifying such targets use human tumor cells grown in vitro or as s.c. xenografts, it is unclear whether such experimental models replica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Camphausen K,Purow B,Sproull M,Scott T,Ozawa T,Deen DF,Tofilon PJ

更新日期：2005-06-07 00:00:00

abstract：:Streptococcus pneumoniae is a major human pathogen that successfully adapts to the host environment via an efficient uptake system for free DNA liberated from other organisms in the upper respiratory tract, facilitating immune evasion and drug resistance. Although the initial signaling events leading to pneumococcal c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balaban M,Bättig P,Muschiol S,Tirier SM,Wartha F,Normark S,Henriques-Normark B

更新日期：2014-02-18 00:00:00

abstract：:RNA polymerase pausing represents an important mechanism of transcriptional regulation. In this study, we use a single-molecule transcription assay to investigate the effect of template base-pair composition on pausing by RNA polymerase II and the evolutionarily distinct mitochondrial polymerase Rpo41. For both enzyme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamft B,Bintu L,Ishibashi T,Bustamante C

更新日期：2012-06-05 00:00:00

abstract：:Tauopathies are a class of neurodegenerative diseases associated with pathological tau. Despite many advances in our understanding of these diseases, the direct mechanism through which tau contributes to neurodegeneration remains poorly understood. Previously, our laboratory implicated the histone demethylase LSD1 in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Engstrom AK,Walker AC,Moudgal RA,Myrick DA,Kyle SM,Bai Y,Rowley MJ,Katz DJ

更新日期：2020-11-17 00:00:00

abstract：:Molecular landers are molecules comprising of a central rigid molecular wire maintained above a metallic surface by organic spacers, which allows specific ultrahigh vacuum-scanning tunneling microscopy experiments to be performed at the single-molecule level. The understanding of the molecule-surface interactions, int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moresco F,Gourdon A

更新日期：2005-06-21 00:00:00

abstract：:The primary structure of the Escherichia coli hemolysin polypeptide (HlyA) is used to predict intramolecular structures involved in the secretion and cytolytic activity of the molecule. The C-terminal region of HlyA contains a repeated, 8-amino acid chain represented by the consensus sequence Leu-Xaa-Gly-Gly-Xaa-Gly-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felmlee T,Welch RA

更新日期：1988-07-01 00:00:00

abstract：:Combined effects of two drugs present simultaneously are usually expressed as summation, synergism or potentiation, and antagonism. When the sum of the effects of each drug present separately equals the combined effect of the two drugs present simultaneously, the action is called additive or summation. However, the ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kamaya H,Ueda I,Eyring H

更新日期：1976-06-01 00:00:00

abstract：:Quaking(viable) (qk(v)) is a recessive neurological mouse mutation with severe dysmyelination of the CNS and spermiogenesis failure. The molecular lesion in the qk(v) mutant is a deletion of approximately 1 Mb on mouse chromosome 17 that alters the expression of the qk gene in oligodendrocytes. Complementation analysi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lorenzetti D,Bishop CE,Justice MJ

更新日期：2004-06-01 00:00:00

abstract：:Neuropathy target esterase (NTE) is a neuronal membrane protein originally identified for its property to be modified by organo-phosphates (OPs), which in humans cause neuropathy characterized by axonal degeneration. Drosophila mutants for the homolog gene of NTE, swisscheese (sws), indicated a possible involvement of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akassoglou K,Malester B,Xu J,Tessarollo L,Rosenbluth J,Chao MV

更新日期：2004-04-06 00:00:00

abstract：:Human T cell leukemia virus type 1 (HTLV-1) has evolved a remarkable strategy to thwart the antiviral effects of the cellular cytidine deaminase APOBEC3G (hA3G). HTLV-1 infects T lymphocytes in vivo, where, like HIV-1, it is likely to encounter hA3G. HIV-1 counteracts the innate antiviral activity of hA3G by producing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Derse D,Hill SA,Princler G,Lloyd P,Heidecker G

更新日期：2007-02-20 00:00:00

abstract：:Treatment of a human breast cancer cell line (MDA-MB-435) in nude mice with a recombinant adenovirus containing the human interferon (IFN) consensus gene, IFN-con1 (ad5/IFN), resulted in tumor regression in 100% of the animals. Tumor regression occurred when virus was injected either within 24 hr of tumor cell implant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang JF,Hu C,Geng Y,Selm J,Klein SB,Orazi A,Taylor MW

更新日期：1996-04-30 00:00:00

abstract：:Sparse sensor placement is a central challenge in the efficient characterization of complex systems when the cost of acquiring and processing data is high. Leading sparse sensing methods typically exploit either spatial or temporal correlations, but rarely both. This work introduces a sparse sensor optimization that i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohren TL,Daniel TL,Brunton SL,Brunton BW

更新日期：2018-10-16 00:00:00

abstract：:We describe the partial purification of an endonuclease from calf thymus that nicks phage PM2 DNA irradiated with UV doses producing only a few pyrimidine dimers per molecule. It has much less activity on DNA that has been subjected to enzymatic photoreactivation after UV irradiation. The calf thymus endonuclease is d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waldstein EA,Peller S,Setlow RB

更新日期：1979-08-01 00:00:00

abstract：:We have compared the efficiency of transformation by linear DNA fragments in yeast strains carrying different numbers of homologous targets for recombination. In strains carrying dispersed copies of a target and in strains carrying tandem arrays, the frequency of transformation is proportional to the number of targets...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson JH,Leung WY,Bosco G,Dieu D,Haber JE

更新日期：1994-01-04 00:00:00

abstract：:Most marine organisms disperse via ocean currents as larvae, so it is often assumed that larval-stage duration is the primary determinant of geographic range size. However, empirical tests of this relationship have yielded mixed results, and alternative hypotheses have rarely been considered. Here we assess the relati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luiz OJ,Allen AP,Robertson DR,Floeter SR,Kulbicki M,Vigliola L,Becheler R,Madin JS

更新日期：2013-10-08 00:00:00