Deletion of the Parkin coregulated gene causes male sterility in the quaking(viable) mouse mutant.

abstract：:Phenotypic modification of dorsal root ganglion (DRG) neurons represents an important mechanism underlying neuropathic pain. However, the nerve injury-induced molecular changes are not fully identified. To determine the molecular alterations in a broader way, we have carried out cDNA array on the genes mainly made fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao HS,Huang QH,Zhang FX,Bao L,Lu YJ,Guo C,Yang L,Huang WJ,Fu G,Xu SH,Cheng XP,Yan Q,Zhu ZD,Zhang X,Chen Z,Han ZG,Zhang X

更新日期：2002-06-11 00:00:00

abstract：:Charybdotoxin (ChTX), a K+ channel blocker, depolarizes human peripheral T lymphocytes and renders them insensitive to activation by mitogen. We observed four types of K+ channels in human T cells: one voltage-activated, and three Ca(2+)-activated. To discern the mechanism by which ChTX depolarizes T cells, we examine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leonard RJ,Garcia ML,Slaughter RS,Reuben JP

更新日期：1992-11-01 00:00:00

abstract：:One of the technical bottlenecks in producing nonhuman primate models is that current assisted reproductive techniques, such as in vitro culture and frozen conservation of multicell-stage embryos, often result in poor embryo quality and subsequently lead to low birth rates. We investigated whether pronuclear embryo tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun Q,Dong J,Yang W,Jin Y,Yang M,Wang Y,Wang PL,Hu Y,Tsien JZ

更新日期：2008-09-02 00:00:00

abstract：:The nature of chemical bonds of ruthenium(Ru)-quinine(Q) complexes, mononuclear [Ru(trpy)(3,5-t-Bu(2)Q)(OH(2))](ClO(4))(2) (trpy = 2,2':6',2''-terpyridine, 3,5-di-tert-butyl-1,2-benzoquinone) (1), and binuclear [Ru(2)(btpyan)(3,6-di-Bu(2)Q)(2)(OH(2))](2+) (btpyan = 1,8-bis(2,2':6',2''-terpyrid-4'-yl)anthracene, 3,6-t-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tanaka K,Isobe H,Yamanaka S,Yamaguchi K

更新日期：2012-09-25 00:00:00

abstract：:Cytochrome c oxidase (COX) is a 13-subunit protein complex that catalyzes the last step in mitochondrial electron transfer in mammals. Of the 10 subunits encoded by nuclear DNA (three are mtDNA products), some are expressed as tissue- and/or development-specific isoforms. For COX subunit VIII, previous work showed tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldberg A,Wildman DE,Schmidt TR,Huttemann M,Goodman M,Weiss ML,Grossman LI

更新日期：2003-05-13 00:00:00

abstract：:Echoviruses have been implicated in multiple human disease syndromes, including aseptic meningitis, paralysis, and heart disease, but no animal model is available for studying the pathogenesis of infection. Production of human integrin very late antigen 2, a receptor for echovirus type 1, in transgenic mice conferred ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes SA,Thaker HM,Racaniello VR

更新日期：2003-12-23 00:00:00

abstract：:Artificially aminoacylated suppressor tRNAs were used to introduce photoreactive amino acids into model mitochondrial precursor proteins to probe the environment along the protein import pathway. Amino acids with benzophenone side chains of various lengths [DL-2-amino-3-(p-benzoylphenyl)propanoic acid (1) and DL-2-ami...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanamori T,Nishikawa S,Shin I,Schultz PG,Endo T

更新日期：1997-01-21 00:00:00

abstract：:The Ser52Pro variant of transthyretin (TTR) produces aggressive, highly penetrant, autosomal-dominant systemic amyloidosis in persons heterozygous for the causative mutation. Together with a minor quantity of full-length wild-type and variant TTR, the main component of the ex vivo fibrils was the residue 49-127 fragme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mangione PP,Porcari R,Gillmore JD,Pucci P,Monti M,Porcari M,Giorgetti S,Marchese L,Raimondi S,Serpell LC,Chen W,Relini A,Marcoux J,Clatworthy IR,Taylor GW,Tennent GA,Robinson CV,Hawkins PN,Stoppini M,Wood SP,Pepys

更新日期：2014-01-28 00:00:00

abstract：:Purified influenza viral cores catalyze the entire process of viral RNA transcription, which includes the endonucleolytic cleavage of heterologous RNAs containing cap 1 (m(7)GpppNm) structures to generate capped primers 10-13 nucleotides long, the initiation of transcription via the incorporation of a guanosine residu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ulmanen I,Broni BA,Krug RM

更新日期：1981-12-01 00:00:00

abstract：:Microcin B17 (MccB17) is a 3.1-kDa Escherichia coli antibiotic that contains thiazole and oxazole heterocycles in a peptide backbone. MccB17 inhibits its cellular target, DNA gyrase, by trapping the enzyme in a complex that is covalently bound to double-strand cleaved DNA, in a manner similar to the well-known quinolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamble DB,Miller DA,Heddle JG,Maxwell A,Walsh CT,Hollfelder F

更新日期：2001-07-03 00:00:00

abstract：:We report potent inhibition of the Mg(II).ATP-dependent protein phosphatase, Fc.M, by the regulatory subunit dimer of type II cAMP-dependent protein kinase, RII2. The protein kinase catalytic subunit has no effect on phosphatase activity and is unable to substitute for kinase FA in the kinase FA- and Mg(II).ATP-mediat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jurgensen SR,Chock PB,Taylor S,Vandenheede JR,Merlevede W

更新日期：1985-11-01 00:00:00

abstract：:In the presence of N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-Ala as donor, and either D-[(14)C]alanine, [(14)C]-glycine, or meso-[(3)H]diaminopimelic acid as acceptor, the DD carboxypeptidases from Streptomyces R61 and R39 catalyze a transpeptidation reaction with the release of terminal D-alanine from the donor and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pollock JJ,Ghuysen JM,Linder R,Salton MR,Perkins HR,Nieto M,Leyh-Bouille M,Frere JM,Johnson K

更新日期：1972-03-01 00:00:00

abstract：:An enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions was partially purified approximately 500- to 1000-fold by DEAE-cellulose chromatography, gel filtration on Sephacryl S300, and chromatography on single-stranded DNA-cellulose. The partially purified enzyme did not have any detectable nuclease acti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Symington LS,Kolodner R

更新日期：1985-11-01 00:00:00

abstract：:Amyloid beta-protein (Abeta) is linked to neuronal injury and death in Alzheimer's disease (AD). Of particular relevance for elucidating the role of Abeta in AD is new evidence that oligomeric forms of Abeta are potent neurotoxins that play a major role in neurodegeneration and the strong association of the 42-residue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bitan G,Kirkitadze MD,Lomakin A,Vollers SS,Benedek GB,Teplow DB

更新日期：2003-01-07 00:00:00

abstract：:During response of budding yeast to peptide mating pheromone, the cell becomes markedly polarized and MAPK scaffold protein Ste5 localizes to the resulting projection (shmoo tip). We demonstrated before that this recruitment is essential for sustained MAPK signaling and requires interaction of a pleckstrin homology (P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garrenton LS,Stefan CJ,McMurray MA,Emr SD,Thorner J

更新日期：2010-06-29 00:00:00

abstract：:A translocation involving human chromosome 10, band q24, in a subset of T-cell acute leukemias disrupts a region surrounding the putative oncogene HOX11, which encodes a protein with a homeodomain. The HOX11 protein binds to a specific DNA sequence, it localizes to the cell nucleus, and it transactivates transcription...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dear TN,Sanchez-Garcia I,Rabbitts TH

更新日期：1993-05-15 00:00:00

abstract：:Accumulative aggregation of mutant Huntingtin (Htt) is a primary neuropathological hallmark of Huntington's disease (HD). Currently, mechanistic understanding of the cytotoxicity of mutant Htt aggregates remains limited, and neuroprotective strategies combating mutant Htt-induced neurodegeneration are lacking. Here, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu Y,Li C,Tao X,Brazill JM,Park J,Diaz-Perez Z,Zhai RG

更新日期：2019-09-17 00:00:00

abstract：:Mass cytometry enables an unprecedented number of parameters to be measured in individual cells at a high throughput, but the large dimensionality of the resulting data severely limits approaches relying on manual "gating." Clustering cells based on phenotypic similarity comes at a loss of single-cell resolution and o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shekhar K,Brodin P,Davis MM,Chakraborty AK

更新日期：2014-01-07 00:00:00

abstract：:Here, we report the isolation and characterization of an endogenous peptide ligand of GPR103 from rat brains. The purified peptide was found to be the 43-residue RF-amide peptide QRFP. We also describe two mouse homologues of human GPR103, termed mouse GPR103A and GPR103B. QRFP binds and activates the human GPR103, as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takayasu S,Sakurai T,Iwasaki S,Teranishi H,Yamanaka A,Williams SC,Iguchi H,Kawasawa YI,Ikeda Y,Sakakibara I,Ohno K,Ioka RX,Murakami S,Dohmae N,Xie J,Suda T,Motoike T,Ohuchi T,Yanagisawa M,Sakai J

更新日期：2006-05-09 00:00:00

abstract：:Modern practice for training classification deepnets involves a terminal phase of training (TPT), which begins at the epoch where training error first vanishes. During TPT, the training error stays effectively zero, while training loss is pushed toward zero. Direct measurements of TPT, for three prototypical deepnet a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papyan V,Han XY,Donoho DL

更新日期：2020-10-06 00:00:00

abstract：:The Eemian (last interglacial, 130-115 ka) was likely the warmest of all interglacials of the last 800 ka, with summer Arctic temperatures 3-5 °C above present. Here, we present improved Eemian climate records from central Greenland, reconstructed from the base of the Greenland Ice Sheet Project 2 (GISP2) ice core. Ou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yau AM,Bender ML,Robinson A,Brook EJ

更新日期：2016-08-30 00:00:00

abstract：:Multiple steps of plant growth and development rely on rapid cell elongation during which secretory and endocytic trafficking via the trans-Golgi network (TGN) plays a central role. Here, we identify the ECHIDNA (ECH) protein from Arabidopsis thaliana as a TGN-localized component crucial for TGN function. ECH partiall...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gendre D,Oh J,Boutté Y,Best JG,Samuels L,Nilsson R,Uemura T,Marchant A,Bennett MJ,Grebe M,Bhalerao RP

更新日期：2011-05-10 00:00:00

abstract：:DEAD-box RNA helicase proteins use the energy of ATP hydrolysis to drive the unwinding of duplex RNA. However, the mechanism that couples ATP utilization to duplex RNA unwinding is unknown. We measured ATP utilization and duplex RNA unwinding by DbpA, a non-processive bacterial DEAD-box RNA helicase specifically activ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henn A,Cao W,Licciardello N,Heitkamp SE,Hackney DD,De La Cruz EM

更新日期：2010-03-02 00:00:00

abstract：:Forests play a key role in the carbon balance of terrestrial ecosystems. One of the main uncertainties in global change predictions lies in how the spatiotemporal dynamics of forest productivity will be affected by climate warming. Here we show an increasing influence of climate on the spatial variability of tree grow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shestakova TA,Gutiérrez E,Kirdyanov AV,Camarero JJ,Génova M,Knorre AA,Linares JC,Resco de Dios V,Sánchez-Salguero R,Voltas J

更新日期：2016-01-19 00:00:00

abstract：:Gram-negative bacteria deliver a cadre of virulence factors directly into the cytoplasm of eukaryotic host cells to promote pathogenesis and/or commensalism. Recently, families of virulence proteins have been recognized that function as E3 Ubiquitin-ligases. How these bacterial ligases integrate into the ubiquitin (Ub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levin I,Eakin C,Blanc MP,Klevit RE,Miller SI,Brzovic PS

更新日期：2010-02-16 00:00:00

abstract：:B7 family proteins provide costimulatory signals that regulate T cell responses. Here we report the third set of B7 family-related T cell inhibitory molecules with the identification of a homolog of the B7 family, B7x. It is expressed in immune cells, nonlymphoid tissues, and some tumor cell lines. B7x inhibits cell-c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zang X,Loke P,Kim J,Murphy K,Waitz R,Allison JP

更新日期：2003-09-02 00:00:00

abstract：:Prostaglandin (PG) D2 has been shown to be transformed by human 11-ketoreductase to 9 alpha,11 beta-PGF2, a biologically active metabolite that is produced in vivo. During the course of developing a mass spectrometric assay for 9 alpha,11 beta-PGF2, several compounds with characteristics similar to PGF2 were detected ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wendelborn DF,Seibert K,Roberts LJ 2nd

更新日期：1988-01-01 00:00:00

abstract：:The Dbf4Cdc7 kinase acts at the level of individual origins to promote the initiation of DNA replication. We demonstrate through both immunoprecipitation and two-hybrid assays that a domain comprising the first 296 aa of Dbf4p interacts with Orc2p and Orc3p subunits of the origin recognition complex (ORC). Given that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duncker BP,Shimada K,Tsai-Pflugfelder M,Pasero P,Gasser SM

更新日期：2002-12-10 00:00:00

abstract：:Copper homeostasis is essential for bacterial pathogen fitness and infection, and has been the focus of a number of recent studies. In Salmonella, envelope protection against copper overload and macrophage survival depends on CueP, a major copper-binding protein in the periplasm. This protein is also required to deliv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pezza A,Pontel LB,López C,Soncini FC

更新日期：2016-10-11 00:00:00

abstract：:The amino acid sequence of blastokinin, also called uteroglobin, has been determined by a combined study of both the intact native molecule and the peptide fragments resulting from tryptic and chymotryptic digestions. Sequence analyses performed by automated methods and by sequential digestion with leucine aminopeptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Popp RA,Foresman KR,Wise LD,Daniel JC Jr

更新日期：1978-11-01 00:00:00