Identification of an unconventional E3 binding surface on the UbcH5 ~ Ub conjugate recognized by a pathogenic bacterial E3 ligase.

abstract：:The role of endocytosis in the control of EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts in which dynamin was conditionally depleted. Dynamin is a GTPase shown to play an important role in the control clathrin mediated endocytosis of EGFR and other cell surface receptors. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sousa LP,Lax I,Shen H,Ferguson SM,De Camilli P,Schlessinger J

更新日期：2012-03-20 00:00:00

abstract：:Abnormal tubulofilamentous structures have been identified in electron micrographs of thin sections and negatively stained impression grids prepared from brains of animals with scrapie and other spongiform encephalopathies, and we showed that such tubules contain a core of filamentous structures resembling scrapie-ass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narang HK,Asher DM,Gajdusek DC

更新日期：1988-05-01 00:00:00

abstract：:Dynamic fluxes of s-block metals like potassium, sodium, and calcium are of broad importance in cell signaling. In contrast, the concept of mobile transition metals triggered by cell activation remains insufficiently explored, in large part because metals like copper and iron are typically studied as static cellular n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dodani SC,Domaille DW,Nam CI,Miller EW,Finney LA,Vogt S,Chang CJ

更新日期：2011-04-12 00:00:00

abstract：:Mutations of von Hippel-Lindau disease (VHL) tumor-suppressor gene product (pVHL) are found in patients with dominant inherited VHL syndrome and in the vast majority of sporadic clear cell renal carcinomas. The function of the pVHL protein has not been clarified. pVHL has been shown to form a complex with elongin B an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iwai K,Yamanaka K,Kamura T,Minato N,Conaway RC,Conaway JW,Klausner RD,Pause A

更新日期：1999-10-26 00:00:00

abstract：:Mucolipidosis type IV is an autosomal recessive lysosomal storage disease of unknown etiology that causes severe neurological and ophthalmological abnormalities. In an attempt to obtain insight into the nature of the metabolic abnormality in this disorder, we prospectively evaluated 15 consecutive patients, aged 2 to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schiffmann R,Dwyer NK,Lubensky IA,Tsokos M,Sutliff VE,Latimer JS,Frei KP,Brady RO,Barton NW,Blanchette-Mackie EJ,Goldin E

更新日期：1998-02-03 00:00:00

abstract：:Three-dimensional topological insulators (3D TIs) represent states of quantum matters in which surface states are protected by time-reversal symmetry and an inversion occurs between bulk conduction and valence bands. However, the bulk-band inversion, which is intimately tied to the topologically nontrivial nature of 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen ZG,Chen RY,Zhong RD,Schneeloch J,Zhang C,Huang Y,Qu F,Yu R,Li Q,Gu GD,Wang NL

更新日期：2017-01-31 00:00:00

abstract：:The yeast AAA-ATPase Cdc48 and the ubiquitin fusion degradation (UFD) proteins play important, evolutionarily conserved roles in ubiquitin dependent protein degradation. The N-terminal domain of Cdc48 interacts with substrate-recruiting cofactors, whereas the C terminus of Cdc48 binds to proteins such as Ufd3 that pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao G,Li G,Schindelin H,Lennarz WJ

更新日期：2009-09-22 00:00:00

abstract：:Ubiquitin E3 ligases are crucial for eliminating misfolded proteins before they form cytotoxic aggregates that threaten cell fitness and survival. However, it remains unclear how emerging misfolded proteins in the cytoplasm can be selectively recognized and eliminated by E3 ligases in plants. We found that Misfolded P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim JH,Cho SK,Oh TR,Ryu MY,Yang SW,Kim WT

更新日期：2017-11-14 00:00:00

abstract：:Erythropoietin, the hormone that is the primary regulator of erythrocyte production, has been purified from anemic-sheep plasma, with a purification factor of more than 1 million. Gel electrophoresis shows that the major contaminant is desialated erythropoietin. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldwasser E,Kung CK

更新日期：1971-04-01 00:00:00

abstract：:The colligative properties of ATP and catecholamines demonstrated in vitro are thought to be responsible for the extraordinary accumulation of solutes inside chromaffin cell secretory vesicles, although this has yet to be demonstrated in living cells. Because functional cells cannot be deprived of ATP, we have knocked...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Estévez-Herrera J,Domínguez N,Pardo MR,González-Santana A,Westhead EW,Borges R,Machado JD

更新日期：2016-07-12 00:00:00

abstract：:Synthesis of the iron-storage protein ferritin is thought to be regulated at the translational level by the cytosolic content of chelatable iron. This response to iron is regulated by the iron-modulated binding to ferritin mRNAs of a repressor protein, the iron regulatory element-binding protein. From measurements mad...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisenstein RS,Garcia-Mayol D,Pettingell W,Munro HN

更新日期：1991-02-01 00:00:00

abstract：:Shigella sonnei is a human-adapted pathogen that is emerging globally as the dominant agent of bacterial dysentery. To investigate local establishment, we sequenced the genomes of 263 Vietnamese S. sonnei isolated over 15 y. Our data show that S. sonnei was introduced into Vietnam in the 1980s and has undergone locali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holt KE,Thieu Nga TV,Thanh DP,Vinh H,Kim DW,Vu Tra MP,Campbell JI,Hoang NV,Vinh NT,Minh PV,Thuy CT,Nga TT,Thompson C,Dung TT,Nhu NT,Vinh PV,Tuyet PT,Phuc HL,Lien NT,Phu BD,Ai NT,Tien NM,Dong N,Parry CM,Hie

更新日期：2013-10-22 00:00:00

abstract：:The highly ordered and reproducible structure of the fungal prion HET-s makes it an excellent model system for studying the inherent properties of prions, self-propagating infectious proteins that have been implicated in a number of fatal diseases. In particular, the HET-s prion-forming domain readily folds into a rel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wan W,Stubbs G

更新日期：2014-04-08 00:00:00

abstract：:We analyzed a very large set of molecular interactions that had been derived automatically from biological texts. We found that published statements, regardless of their verity, tend to interfere with interpretation of the subsequent experiments and, therefore, can act as scientific "microparadigms," similar to domina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rzhetsky A,Iossifov I,Loh JM,White KP

更新日期：2006-03-28 00:00:00

abstract：:A genetic analysis is necessary to gain a greater understanding of the complex developmental processes in mammals. Toward this end, an insertional transgenic mouse mutant has been isolated that results in abnormal germ-cell development. This recessive mutation manifests as infertility in both males and females and is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pellas TC,Ramachandran B,Duncan M,Pan SS,Marone M,Chada K

更新日期：1991-10-01 00:00:00

abstract：:Goblet cell metaplasia and mucus hypersecretion are observed in many pulmonary diseases, including asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis. However, the regulation of goblet cell differentiation remains unclear. Here, we identify a regulator of this process in an N-ethyl-N-nitrosourea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HT,Yin W,Nakamichi Y,Panza P,Grohmann B,Buettner C,Guenther S,Ruppert C,Kobayashi Y,Guenther A,Stainier DYR

更新日期：2019-12-17 00:00:00

abstract：:To characterize the region on human IgG1 responsible for its high-affinity interaction with the human Fc gamma receptor class I (Fc gamma RI), we have analyzed the binding properties of a series of genetically engineered chimeric antidinitrophenyl antibodies with identical murine antibody combining sites and hybrid Ig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chappel MS,Isenman DE,Everett M,Xu YY,Dorrington KJ,Klein MH

更新日期：1991-10-15 00:00:00

abstract：:DNA sequences containing short adenine tracts are intrinsically curved and play a role in transcriptional regulation. Despite many high-resolution NMR and x-ray studies, the origins of curvature remain disputed. Long-range restraints provided by 85 residual dipolar couplings were measured for a DNA decamer containing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbic A,Zimmer DP,Crothers DM

更新日期：2003-03-04 00:00:00

abstract：:Nuclear bodies are RNA and protein-rich, membraneless organelles that play important roles in gene regulation. The largest and most well-known nuclear body is the nucleolus, an organelle whose primary function in ribosome biogenesis makes it key for cell growth and size homeostasis. The nucleolus and other nuclear bod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berry J,Weber SC,Vaidya N,Haataja M,Brangwynne CP

更新日期：2015-09-22 00:00:00

abstract：:The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized "late" polyoma-specific RNA appears as "giant" molecules of heterogeneous size, up to several times large...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acheson NH,Buetti E,Scherrer K,Weil R

更新日期：1971-09-01 00:00:00

abstract：:The size of an organism reflects its metabolic rate, growth rate, mortality, and other important characteristics; therefore, the distribution of body size is a major determinant of ecosystem structure and function. Body-size distributions often are multimodal, with several peaks of abundant sizes, and previous studies...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lampert A,Tlusty T

更新日期：2013-01-02 00:00:00

abstract：:The history of the genus Pan is a topic of enduring interest. Chimpanzees (Pan troglodytes) are often divided into subspecies, but the population structure and genetic history of chimpanzees across Africa remain unclear. Some population genetics studies have led to speculation that, until recently, this species consti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gonder MK,Locatelli S,Ghobrial L,Mitchell MW,Kujawski JT,Lankester FJ,Stewart CB,Tishkoff SA

更新日期：2011-03-22 00:00:00

abstract：:Ring-shaped hexameric helicases and translocases support essential DNA-, RNA-, and protein-dependent transactions in all cells and many viruses. How such systems coordinate ATPase activity between multiple subunits to power conformational changes that drive the engagement and movement of client substrates is a fundame...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomsen ND,Lawson MR,Witkowsky LB,Qu S,Berger JM

更新日期：2016-11-29 00:00:00

abstract：:The enhanced disease resistance 1 (edr1) mutation of Arabidopsis confers resistance to powdery mildew disease caused by the fungus Erysiphe cichoracearum. Resistance mediated by the edr1 mutation is correlated with induction of several defense responses, including host cell death. Double mutant analysis revealed that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frye CA,Tang D,Innes RW

更新日期：2001-01-02 00:00:00

abstract：:Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract x-rays to at least 4.5-A resolution. The subfragment 1 crystallizes in space group P2(1)2(1)2(1) where a = 107 A, b = 117 A, and c = 278 A. The cell dimensions and intensity distribution on x-ray diffraction photogr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rayment I,Winkelmann DA

更新日期：1984-07-01 00:00:00

abstract：:Historically, rate constants were determined in vitro and it was unknown whether they were valid for in vivo biological processes. Here, we bridge this gap by measuring binding dynamics between a pair of proteins in living HeLa cells. Binding of a β-lactamase to its protein inhibitor was initiated by microinjection an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillip Y,Kiss V,Schreiber G

更新日期：2012-01-31 00:00:00

abstract：:Surface proteins of mouse thymus and spleen cells were radioiodinated with lactoperoxidase. After solubilization, the labeled proteins were precipitated by antibodies directed against mouse immunoglobulin chains; the precipitates were analyzed by radioautography after Na dodecyl sulfate-gel electrophoresis. Radioactiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lisowska-Bernstein B,Rinuy A,Vassalli P

更新日期：1973-10-01 00:00:00

abstract：:Most processing of sensation involves the cortical hemisphere opposite (contralateral) to the stimulated limb. Stroke patients can exhibit changes in the interhemispheric balance of sensory signal processing. It is unclear whether these changes are the result of poststroke rewiring and experience, or whether they coul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohajerani MH,Aminoltejari K,Murphy TH

更新日期：2011-05-31 00:00:00

abstract：:Cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) belong to the Cucumovirus genus. They have a tripartite genome consisting of single-stranded RNAs, designated 1, 2, and 3. Previous studies have shown that viable pseudorecombinants could be created in vitro by reciprocal exchanges between CMV and TAV RNA 3, b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masuta C,Ueda S,Suzuki M,Uyeda I

更新日期：1998-09-01 00:00:00

abstract：:Single crystal diamond produced by chemical vapor deposition (CVD) at very high growth rates (up to 150 microm/h) has been successfully annealed without graphitization at temperatures up to 2200 degrees C and pressures <300 torr. Crystals were annealed in a hydrogen environment by using microwave plasma techniques for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng YF,Yan CS,Lai J,Krasnicki S,Shu H,Yu T,Liang Q,Mao HK,Hemley RJ

更新日期：2008-11-18 00:00:00