Abstract：
:DNA-based nanopores are synthetic biomolecular membrane pores, whose geometry and chemical functionality can be tuned using the tools of DNA nanotechnology, making them promising molecular devices for applications in single-molecule biosensing and synthetic biology. Here we introduce a large DNA membrane channel with an ≈4 nm diameter pore, which has stable electrical properties and spontaneously inserts into flat lipid bilayer membranes. Membrane incorporation is facilitated by a large number of hydrophobic functionalizations or, alternatively, streptavidin linkages between biotinylated channels and lipids. The channel displays an Ohmic conductance of ≈3 nS, consistent with its size, and allows electrically driven translocation of single-stranded and double-stranded DNA analytes. Using confocal microscopy and a dye influx assay, we demonstrate the spontaneous formation of membrane pores in giant unilamellar vesicles. Pores can be created both in an outside-in and an inside-out configuration.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Krishnan S,Ziegler D,Arnaut V,Martin TG,Kapsner K,Henneberg K,Bausch AR,Dietz H,Simmel FCdoi
10.1038/ncomms12787subject
Has Abstractpub_date
2016-09-23 00:00:00pages
12787issn
2041-1723pii
ncomms12787journal_volume
7pub_type
杂志文章abstract：:The sigma-2 receptor, whose gene remains to be cloned, has been validated as a biomarker for tumour cell proliferation. Here we report the use of a novel photoaffinity probe, WC-21, to identify the sigma-2 receptor-binding site. WC-21, a sigma-2 ligand containing both a photoactive azide moiety and a fluorescein isoth...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms1386
更新日期：2011-07-05 00:00:00
abstract：:CD8+ T cells provide a critical defence from pathogens at mucosal epithelia including the female reproductive tract (FRT). Mucosal immunisation is considered essential to initiate this response, however this is difficult to reconcile with evidence that antigen delivered to skin can recruit protective CD8+ T cells to m...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-09969-2
更新日期：2019-05-17 00:00:00
abstract：:A topological insulator is the state of quantum matter possessing gapless spin-locking surface states across the bulk band gap, which has created new opportunities from novel electronics to energy conversion. However, the large concentration of bulk residual carriers has been a major challenge for revealing the proper...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms1771
更新日期：2012-03-27 00:00:00
abstract：:Microbial community structure can be analyzed by quantifying cell numbers or by quantifying biomass for individual populations. Methods for quantifying cell numbers are already available (e.g., fluorescence in situ hybridization, 16-S rRNA gene amplicon sequencing), yet high-throughput methods for assessing community ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-01544-x
更新日期：2017-11-16 00:00:00
abstract：:Competing interactions in the so-called spin-ice compounds stabilize a frustrated ground state with finite zero-point entropy and, interestingly, emergent magnetic monopole excitations. The properties of these monopoles are at the focus of recent research with particular emphasis on their quantum dynamics. It is predi...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms5853
更新日期：2014-09-04 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease, characterised by increased expression of type I interferon (IFN)-regulated genes and a striking sex imbalance towards females. Through combined genetic, in silico, in vitro, and ex vivo approaches, we define CXorf21, a gene of hitherto unknown function, whic...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-10106-2
更新日期：2019-05-15 00:00:00
abstract：:Evasion of the host phagocyte response by Staphylococcus aureus is crucial to successful infection with the pathogen. γ-haemolysin AB and CB (HlgAB, HlgCB) are bicomponent pore-forming toxins present in almost all human S. aureus isolates. Cellular tropism and contribution of the toxins to S. aureus pathophysiology ar...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms6438
更新日期：2014-11-11 00:00:00
abstract：:Electric eels (Electrophorus electricus) are legendary for their ability to incapacitate fish, humans, and horses with hundreds of volts of electricity. The function of this output as a weapon has been obvious for centuries but its potential role for electroreception has been overlooked. Here it is shown that electric...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms9638
更新日期：2015-10-20 00:00:00
abstract：:Bright solitons are non-dispersive wave solutions, arising in a diverse range of nonlinear, one-dimensional systems, including atomic Bose-Einstein condensates with attractive interactions. In reality, cold-atom experiments can only approach the idealized one-dimensional limit necessary for the realization of true sol...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms2893
更新日期：2013-01-01 00:00:00
abstract：:The description of ultrafast nonadiabatic chemical dynamics during molecular photo-transformations remains challenging because electronic and nuclear configurations impact each other and cannot be treated independently. Here we gain experimental insights, beyond the Born-Oppenheimer approximation, into the light-induc...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms15342
更新日期：2017-05-24 00:00:00
abstract：:Biomechanical energy harvesting textiles based on nanogenerators that convert mechanical energy into electricity have broad application prospects in next-generation wearable electronic devices. However, the difficult-to-weave structure, limited flexibility and stretchability, small device size and poor weatherability ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-08846-2
更新日期：2019-02-20 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Nature communications
pub_type： 已发布勘误
doi：10.1038/s41467-020-15252-6
更新日期：2020-04-08 00:00:00
abstract：:The interpretation of molecular vibrational spectroscopic signals in terms of atomic motion is essential to understand molecular mechanisms and for chemical characterization. The signals are usually assigned after harmonic normal mode analysis, even if molecular vibrations are known to be anharmonic. Here we obtain th...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-18211-3
更新日期：2020-08-28 00:00:00
abstract：:Dark matter-only simulations of galaxy formation predict many more subhalos around a Milky Way-like galaxy than the number of observed satellites. Proposed solutions require the satellites to inhabit dark matter halos with masses 10(9)-10(10 )Msun at the time they fell into the Milky Way. Here we use a modelling appro...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms8599
更新日期：2015-07-02 00:00:00
abstract：:Imputing genotypes from reference panels created by whole-genome sequencing (WGS) provides a cost-effective strategy for augmenting the single-nucleotide polymorphism (SNP) content of genome-wide arrays. The UK10K Cohorts project has generated a data set of 3,781 whole genomes sequenced at low depth (average 7x), aimi...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms9111
更新日期：2015-09-14 00:00:00
abstract：:Considerable interest has been devoted to converting mechanical energy into electricity using polymer nanofibres. In particular, piezoelectric nanofibres produced by electrospinning have shown remarkable mechanical energy-to-electricity conversion ability. However, there is little data for the acoustic-to-electric con...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms11108
更新日期：2016-03-23 00:00:00
abstract：:Oxygen vacancies in proximity to surfaces and heterointerfaces in oxide thin film heterostructures have major effects on properties, resulting, for example, in emergent conduction behaviour, large changes in metal-insulator transition temperatures or enhanced catalytic activity. Here we report the discovery of a means...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms11892
更新日期：2016-06-10 00:00:00
abstract：:Hydrogen spillover phenomenon is well-documented in hydrogenation catalysis but still highly disputed in hydrogen storage. Until now, the existence of hydrogen spillover through metal-organic frameworks (MOFs) remains a topic of ongoing debate and how far the split hydrogen atoms diffuse in such materials is unknown. ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-018-06269-z
更新日期：2018-09-17 00:00:00
abstract：:Active biofluid management is central to the realization of wearable bioanalytical platforms that are poised to autonomously provide frequent, real-time, and accurate measures of biomarkers in epidermally-retrievable biofluids (e.g., sweat). Accordingly, here, a programmable epidermal microfluidic valving system is de...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-18238-6
更新日期：2020-09-02 00:00:00
abstract：:Macrophage-mediated inflammation is thought to have a causal role in osteoarthritis-related pain and severity, and has been suggested to be triggered by endotoxins produced by the gastrointestinal microbiome. Here we investigate the relationship between joint pain and the gastrointestinal microbiome composition, and o...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-12873-4
更新日期：2019-10-25 00:00:00
abstract：:Thrombocytosis is present in more than 30% of patients with solid malignancies and correlates with worsened patient survival. Tumor cell interaction with various cellular components of the tumor microenvironment including platelets is crucial for tumor growth and metastasis. Although it is known that platelets can inf...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-00411-z
更新日期：2017-08-21 00:00:00
abstract：:The xeroderma pigmentosum C (XPC) complex initiates nucleotide excision repair by recognizing DNA lesions before recruiting downstream factors. How XPC detects structurally diverse lesions embedded within normal DNA is unknown. Here we present a crystal structure that captures the yeast XPC orthologue (Rad4) on a sing...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms6849
更新日期：2015-01-06 00:00:00
abstract：:In bulk superfluid 3He at zero magnetic field, two phases emerge with the B-phase stable everywhere except at high pressures and temperatures, where the A-phase is favoured. Aerogels with nanostructure smaller than the superfluid coherence length are the only means to introduce disorder into the superfluid. Here we us...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms12975
更新日期：2016-09-27 00:00:00
abstract：:The exploration of chemical space for new reactivity, reactions and molecules is limited by the need for separate work-up-separation steps searching for molecules rather than reactivity. Herein we present a system that can autonomously evaluate chemical reactivity within a network of 64 possible reaction combinations ...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms15733
更新日期：2017-06-09 00:00:00
abstract：:One of the main reasons why nonlinear-optical signal processing (regeneration, logic, etc.) has not yet become a practical alternative to electronic processing is that the all-optical elements with nonlinear input-output relationship have remained inherently single-channel devices (just like their electronic counterpa...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-017-00874-0
更新日期：2017-10-12 00:00:00
abstract：:Coupling between vibrational modes is essential for energy transfer and dissipation in condensed matter. For water, different O-H stretch modes are known to be very strongly coupled both within and between water molecules, leading to ultrafast dissipation and delocalization of vibrational energy. In contrast, the info...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-020-19759-w
更新日期：2020-11-25 00:00:00
abstract：:Idiopathic pulmonary fibrosis (IPF) is a fatal disease in which the intricate alveolar network of the lung is progressively replaced by fibrotic scars. Myofibroblasts are the effector cells that excessively deposit extracellular matrix proteins thus compromising lung structure and function. Emerging literature suggest...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/s41467-019-10839-0
更新日期：2019-07-05 00:00:00
abstract：:Ultrafast photoinduced electron transfer preceding energy equilibration still poses many experimental and conceptual challenges to the optimization of photoconversion since an atomic-scale description has so far been beyond reach. Here we combine femtosecond transient optical absorption spectroscopy with ultrafast X-r...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms7359
更新日期：2015-03-02 00:00:00
abstract：:The original version of this Article was missing the ORCID ID of Professor Nicola Pugno.Also in the original version of this Article, the third to last sentence of the fourth paragraph of the Results incorrectly read 'However, the stepwise addition of CNTs increases the self-entanglement and thereby the compressive st...
journal_title：Nature communications
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41467-017-02372-9
更新日期：2018-01-09 00:00:00
abstract：:Due to the generally delocalized nature of molecular valence orbitals, valence-shell spectroscopies do not usually allow to specifically target a selected atom in a molecule. However, in X-ray electron spectroscopy, the photoelectron momentum is large and the recoil angular momentum transferred to the molecule is larg...
journal_title：Nature communications
pub_type： 杂志文章
doi：10.1038/ncomms4816
更新日期：2014-05-09 00:00:00
