Abstract:
:Autophagy, a catabolic pathway of lysosomal degradation, acts not only as an efficient recycle and survival mechanism during cellular stress, but also as an anti-infective machinery. The human pathogen Staphylococcus aureus (S. aureus) was originally considered solely as an extracellular bacterium, but is now recognized additionally to invade host cells, which might be crucial for persistence. However, the intracellular fate of S. aureus is incompletely understood. Here, we show for the first time induction of selective autophagy by S. aureus infection, its escape from autophagosomes and proliferation in the cytoplasm using live cell imaging. After invasion, S. aureus becomes ubiquitinated and recognized by receptor proteins such as SQSTM1/p62 leading to phagophore recruitment. Yet, S. aureus evades phagophores and prevents further degradation by a MAPK14/p38α MAP kinase-mediated blockade of autophagy. Our study demonstrates a novel bacterial strategy to block autophagy and secure survival inside the host cell.
journal_name
Autophagyjournal_title
Autophagyauthors
Neumann Y,Bruns SA,Rohde M,Prajsnar TK,Foster SJ,Schmitz Idoi
10.1080/15548627.2016.1226732subject
Has Abstractpub_date
2016-11-01 00:00:00pages
2069-2084issue
11eissn
1554-8627issn
1554-8635journal_volume
12pub_type
杂志文章相关文献
Autophagy文献大全abstract::Autophagy as a natural part of cellular homeostasis usually takes place unnoticed by neighboring cells. However, its co-occurrence with cell death may contribute to the clearance of these dying cells by recruited phagocytes. Autophagy associated with programmed cell death has recently been reported to be essential for...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.4731
更新日期:2007-09-01 00:00:00
abstract::The NIH-funded center for autophagy research named Autophagy, Inflammation, and Metabolism (AIM) Center of Biomedical Research Excellence, located at the University of New Mexico Health Science Center is now completing its second year as a working center with a mission to promote autophagy research locally, nationally...
journal_title:Autophagy
pub_type: 历史文章,杂志文章
doi:10.1080/15548627.2019.1634444
更新日期:2019-10-01 00:00:00
abstract::The conserved target of rapamycin (TOR) kinase is a central regulator of cell growth in response to nutrient availability. TOR forms 2 structurally and functionally distinct complexes, TORC1 and TORC2, and negatively regulates autophagy via TORC1. Here we demonstrate TOR also operates independently through the TORC2 s...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.36262
更新日期:2014-01-01 00:00:00
abstract::Mitochondrial morphological and structural changes play a role in several cellular processes, including apoptosis. We recently reported that mitochondrial elongation is also critical to sustain cell viability during macroautophagy. During macroautophagy unopposed mitochondrial fusion leads to organelle elongation both...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.7.10.16771
更新日期:2011-10-01 00:00:00
abstract::The Atg8-family proteins are subdivided into two subfamilies: the GABARAP and LC3 subfamilies. These proteins, which are major players of the autophagy pathway, present a conserved glycine in their C-terminus necessary for their association to the autophagosome membrane. This family of proteins present multiple roles ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1749367
更新日期:2020-04-19 00:00:00
abstract::Deficiency in decidualization has been widely regarded as an important cause of spontaneous abortion. Generalized decidualization also includes massive infiltration and enrichment of NK cells. However, the underlying mechanism of decidual NK (dNK) cell residence remains largely unknown. Here, we observe that the incre...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1833515
更新日期:2020-11-01 00:00:00
abstract::IRGM is a genetic risk factor for several autoimmune diseases. However, the mechanism of IRGM-mediated protection in autoimmunity remains undetermined. The abnormal activation of type I interferon (IFN) response is one of the significant factors in the pathogenesis of several autoimmune diseases. In our recent study, ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2020.1810920
更新日期:2020-08-19 00:00:00
abstract::The elimination of tumor cells by apoptosis is the main mechanism of action of chemotherapeutic drugs used in current treatment protocols of acute lymphoblastic leukemia (ALL). Despite the rapid cytoreduction achieved, serious acute and late complications are frequent, and resistance to chemotherapy develops. During t...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.5.8507
更新日期:2009-07-01 00:00:00
abstract::During reticulocyte maturation, hematopoietic progenitors undergo numerous changes to reach the final functional stage which concludes with the release of reticulocytes and erythrocytes into circulation. During this process some proteins, which are not required in the mature stage, are sequestered in the internal vesi...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.2.2.2350
更新日期:2006-04-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of upper and lower motor neurons. As with other age-dependent neurodegenerative disorders, ALS is linked to the presence of misfolded proteins that may perturb several intracellular mechanisms and trigger neu...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.7.13042
更新日期:2010-10-01 00:00:00
abstract::Macroautophagy/autophagy dysfunction is associated with many neurodegenerative diseases. TFEB (transcription factor EB), an important molecule that regulates lysosomal and autophagy function, is regarded as a potential target for treating some neurodegenerative diseases. However, the relationship between autophagy dys...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2019.1569926
更新日期:2019-06-01 00:00:00
abstract::Adaptive immunity, which plays an important role in the development of atherosclerosis, is mediated by major histocompatibility complex (MHC)-dependent antigen presentation. In atherosclerotic lesions, macrophages constitute an important class of antigen-presenting cells that activate adaptive immune responses to oxid...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1037061
更新日期:2015-01-01 00:00:00
abstract::Ferroptosis is a form of regulated cell death caused by iron accumulation and oxidative injury. BECN1 is a key regulator of macroautophagy/autophagy, a catabolic process of degradation induced by starvation or other stressors. Our recent findings reveal a novel alternative mechanism by which BECN1 can promote ferropto...
journal_title:Autophagy
pub_type: 评论,杂志文章
doi:10.1080/15548627.2018.1513758
更新日期:2018-01-01 00:00:00
abstract::Recent evidence suggests that autophagy may favor fibrosis through enhanced differentiation of fibroblasts in myofibroblasts. Here, we sought to characterize the mediators and signaling pathways implicated in autophagy-induced myofibroblast differentiation. Fibroblasts, serum starved for up to 4 d, showed increased LC...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/15548627.2014.981786
更新日期:2014-01-01 00:00:00
abstract::Autophagy and microRNA (miRNA) are important regulators during cancer cell tumorigenesis. Impaired autophagy and high expression of the oncogenic microRNA MIR224 are prevalent in hepatocellular carcinoma (HCC); however, the relationship between the 2 phenomena remains elusive. In this study, we are the first to reveal...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.29959
更新日期:2014-09-01 00:00:00
abstract::The RNA replication complexes of small positive-strand RNA viruses such as poliovirus are known to form on the surfaces of membranous vesicles in the cytoplasm of infected mammalian cells. These membranes resemble cellular autophagosomes in their double-membraned morphology, cytoplasmic lumen, lipid-rich composition a...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.5377
更新日期:2008-04-01 00:00:00
abstract::The islet in type 2 diabetes mellitus (T2DM) is characterized by a deficit in β-cells and increased β-cell apoptosis attributable at least in part to intracellular toxic oligomers of IAPP (islet amyloid polypeptide). β-cells of individuals with T2DM are also characterized by accumulation of polyubiquitinated proteins ...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.28478
更新日期:2014-06-01 00:00:00
abstract::Radiation and conventional cytotoxic chemotherapies are ineffective in treating renal cancer. Approximately 75 percent of renal cell carcinoma (RCC) is associated with an inactivation of the tumor suppressor gene von Hippel-Lindau (VHL). We exploited the possibility of targeting VHL-deficient RCC through synthetic let...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6785
更新日期:2008-10-01 00:00:00
abstract::When no supply of environmental nutrients is available, cells induce autophagy, thereby generating a source of emergency metabolic substrates and energy to maintain the basal cellular activity needed for survival. This autophagy response to starvation has been well characterized in various multicellular organisms, inc...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.4.8171
更新日期:2009-05-01 00:00:00
abstract::Autophagy is a catabolic process by which cells remove unwanted proteins and damaged organelles. It is important for maintaining cellular homeostasis and can also be used by cells to remove intracellular microbial pathogens. As such, some viruses such as herpes simplex virus-1 (HSV-1) have evolved mechanisms to suppre...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.4.11669
更新日期:2010-05-01 00:00:00
abstract::The role of autophagy, a catabolic lysosome-dependent pathway, has recently been recognized in a variety of disorders, including Pompe disease, which results from a deficiency of the glycogen-degrading lysosomal hydrolase acid-alpha glucosidase (GAA). Skeletal and cardiac muscle are most severely affected by the progr...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.1.7293
更新日期:2009-01-01 00:00:00
abstract::Chronic lung inflammation is accepted as being associated with the development of lung cancer caused by nickel exposure. Therefore, identifying the molecular mechanisms that lead to a nickel-induced sustained inflammatory microenvironment that causes transformation of human bronchial epithelial cells is of high signif...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1196313
更新日期:2016-10-02 00:00:00
abstract::The lack of a rapid and quantitative autophagy assay has substantially hindered the development and implementation of autophagy-targeting therapies for a variety of human diseases. To address this critical issue, we developed a novel autophagy assay using the newly developed Cyto-ID fluorescence dye. We first verified...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2015.1017181
更新日期:2015-01-01 00:00:00
abstract::Complex membrane trafficking events are involved in the regulation of antigen processing and presentation of both endogenous and exogenous antigens. While these processes were believed to involve mainly organelles along the endo/phagocytic and the biosynthetic pathways, recent studies have shown that autophagy also pa...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.7.9163
更新日期:2009-10-01 00:00:00
abstract::Autophagy is essential for prolonging yeast survival during nutrient deprivation; however, this report shows that some autophagy proteins may also be accelerating population death in those conditions. While leucine starvation caused YCA1-mediated apoptosis characterized by increased annexin V staining, nitrogen depriv...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.19314
更新日期:2012-05-01 00:00:00
abstract::Autophagy is one of the survival processes of cancer cells, especially in stressful conditions such as starvation, hypoxia and chemotherapeutic agents. However, its roles in tumor survival have not yet been fully elucidated. Here, we found for the first time that JAK2/STAT3 was activated in HeLa cells when they were s...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.6.8.13547
更新日期:2010-11-01 00:00:00
abstract::One of the major side effects of cisplatin chemotherapy is toxic acute kidney injury due to preferential accumulation of cisplatin in renal proximal tubule epithelial cells and the subsequent injury to these cells. Apoptosis is known as a major mechanism of cisplatin-induced cell death in renal tubular cells. We have ...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.6309
更新日期:2008-07-01 00:00:00
abstract::Autophagy has recently been implicated in the host defense against the intracellular protozoan pathogen, Toxoplasma gondii, a major opportunistic pathogen of the central nervous system in immunosuppressed individuals. In both IFN gamma-activated macrophages and astrocytes, the p47 GTPases traffic to the T. gondii para...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.4161/auto.5.2.7637
更新日期:2009-02-01 00:00:00
abstract::The N-terminal amino acid of a protein is an essential determinant of ubiquitination and subsequent proteasomal degradation in the N-end rule pathway. Using para-chloroamphetamine (PCA), a specific inhibitor of the arginylation branch of the pathway (Arg/N-end rule pathway), we identified that blocking the Arg/N-end r...
journal_title:Autophagy
pub_type: 杂志文章
doi:10.1080/15548627.2016.1222991
更新日期:2016-11-01 00:00:00
abstract::The Atg1/ULK1 complex plays a central role in starvation-induced autophagy, integrating signals from upstream sensors such as MTOR and AMPK and transducing them to the downstream autophagy pathway. Much progress has been made in the last few years in understanding the mechanisms by which the complex is regulated throu...
journal_title:Autophagy
pub_type: 杂志文章,评审
doi:10.4161/auto.23323
更新日期:2013-02-01 00:00:00