Abstract：
:In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Tanackovic V,Rydahl MG,Pedersen HL,Motawia MS,Shaik SS,Mikkelsen MD,Krunic SL,Fangel JU,Willats WG,Blennow Adoi
10.1038/srep30551subject
Has Abstractpub_date
2016-07-29 00:00:00pages
30551issn
2045-2322pii
srep30551journal_volume
6pub_type
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