Abstract:
:Bone-marrow-derived macrophages are divided into two phenotypically and functionally distinct subsets, M1 and M2 macrophages. Recently, it was shown that adoptive transfer of M2-polarized peritoneal macrophages reduced the severity of experimental colitis in mice. However, it is still unclear whether peritoneal macrophages possess the same ability to be polarized to cells with functionally different phenotypes and cytokine production patterns as bone-marrow-derived macrophages. To address this question, we examined the ability of peritoneal macrophages to be polarized to the M1 and M2 phenotypes and determined the specific cytokine profiles of cells with each phenotype. We showed that peritoneal macrophages, as well as bone-marrow-derived macrophages, were differentiated into M1 and M2 phenotypes following stimulation with interferon-γ (IFN-γ) and interleukin-4 (IL-4)/IL-13, respectively. Following in vitro stimulation with lipopolysaccharide, M2-polarized peritoneal macrophages predominantly expressed T helper type 2 (Th2) cytokines and regulatory cytokines, including IL-4, IL-13, transforming growth factor-β and IL-10, whereas M1-polarized peritoneal macrophages expressed negligible amounts of Th1 and pro-inflammatory cytokines. ELISA showed that M2-polarized peritoneal macrophages produced significantly more IL-10 than M1-polarized peritoneal macrophages. Notably, M2-polarized peritoneal macrophages contributed more to the suppression of T-cell proliferation than did M1-polarized peritoneal macrophages. The mRNA expression of Th2 cytokines, including IL-4 and IL-13, increased in T-cells co-cultured with M2-polarized macrophages. Hence, our findings showed that M2 polarization of peritoneal macrophages induced regulatory cytokine production and suppressed T-cell proliferation in vitro, and that resident peritoneal macrophages could be used as a new adoptive transfer therapy for autoimmune/inflammatory diseases after polarization to the regulatory phenotype ex vivo.
journal_name
Immunologyjournal_title
Immunologyauthors
Oishi S,Takano R,Tamura S,Tani S,Iwaizumi M,Hamaya Y,Takagaki K,Nagata T,Seto S,Horii T,Osawa S,Furuta T,Miyajima H,Sugimoto Kdoi
10.1111/imm.12647subject
Has Abstractpub_date
2016-11-01 00:00:00pages
320-328issue
3eissn
0019-2805issn
1365-2567journal_volume
149pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Using the fluorescence-activated cell sorter (FACS), the percentages of T and B lymphocytes in the peripheral blood, spleen and lymph nodes of CBA syngeneic and allogeneic pregnancies were determined. At 19 days of syngeneic pregnancy, the level of T cells in the blood had decreased and that of B cells had increased. ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-04-01 00:00:00
abstract::Veiled cells (VC), isolated from the thoracic duct of irradiated lymphadenectomized mice (MLNX) or peritoneal macrophages (PM phi were incubated with isotope-labelled hen egg lysozyme (HEL), purified protein derivative (PPD) or keyhole limpet haemocyanin (KLH) in vitro. About 2-10 times less antigen was associated wit...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Stimulation of human polymorphonuclear leucocytes (PMN) with phagocytosable particles [yeast-IgG (Y-IgG)], lipopolysaccharide (LPS), tumour necrosis factor (TNF) or formyl-methionyl-leucyl-phenyl-alanine (FMLP) results in an increase of the interleukin-8 (IL-8) mRNA accumulation and a subsequent release of the protein...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::A suspension of nucleated femoral (BALB/c x CBA) bone marrow cells was inoculated, either into glass flasks or into flasks coated with a preformed syngeneic marrow derived monolayer, and cultured for long periods in media supplemented with hydrocortisone sodium succinate. Long term analysis of the lymphoid status of t...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Mice fed a protein-deficient diet containing 2% ovalbumin for 6 weeks (PD) show a reduced spleen IgA plaque-forming cell (PFC) response after oral immunization with sheep red blood cells (SRBC), compared to the response of mice fed a control diet containing 20% ovalbumin (C). This reduced IgA PFC response was partiall...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
abstract::Factors that influence the generation and maintenance of memory CD8+ T cells are not fully understood. The homeostasis of memory T cells is highly dynamic and tightly regulated by various stimuli, including cytokines and antigen-major histocompatibility complex ligands. We characterized the hepatitis C virus (HCV)-spe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2003.01769.x
更新日期:2003-12-01 00:00:00
abstract::HIV infection is associated with increased representation of T cells bearing an activated, memory (CD45RO+) phenotype. Although administration of antiretroviral agents and interleukin-2 (IL-2) augment depleted CD4+ T-cell numbers, such therapies have been preferentially beneficial for CD45RO+ T cells. Interleukin-15 (...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00807.x
更新日期:1999-06-01 00:00:00
abstract::CD45 is known to regulate signalling through many different surface receptors in diverse haemopoietic cell types. Here we report for the first time that CD45-/- bone marrow dendritic cells (BMDC) are more activated than CD45+/+ cells and that tumour necrosis factor (TNF) and interleukin-6 (IL-6) production by BMDC and...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02363.x
更新日期:2006-06-01 00:00:00
abstract::Cutaneous delayed hypersensitivity reactions to collagen in guinea-pigs were partially but specifically suppressed if the animals had been pretreated with collagen and Freund's incomplete adjuvant. Such animals responded normally to skin-reactive factor prepared with ovalbumin. Lymphoid cells from animals with normal ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-07-01 00:00:00
abstract::The pathology of the spleen, lymph nodes and thymus of C57L/J mice, infected intraperitoneally with 20 and 100 cysts of Echinococcus multilocularis is described at 2, 4, 8 and 12 weeks postinfection (p.i.). For the first 8 weeks, growth of the larval cyst mass (LCM) was slow and blastogenesis in T-dependent areas of b...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::Natural cell-mediated cytotoxicity is mediated by a family of effector cells that express cytolytic activities distinct from those generally attributed to B cells, T cells and macrophages; it includes both natural killer (NK) and natural cytotoxic (NC) activities. There is now convincing evidence to show that NC activ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::This paper uses B-lymphocyte cloning methods to quantify the effects of anti-mu chain antibody on immature and mature B cells. Nude mouse spleen lymphocytes were incubated with various concentrations of sheep anti-mouse mu chain antibody for times varying from 10 min to 24 h. They were then washed and plated in the ag...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract::Runx1 transcription factor is highly expressed at a CD4/CD8-double-negative (DN) stage of thymocyte development but is down-regulated when cells proceed to the double-positive (DP) stage. In the present study, we examined whether the down-regulation of Runx1 is necessary for thymocyte differentiation from the DN to DP...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03230.x
更新日期:2010-06-01 00:00:00
abstract::Lysine prevented the activation of Cl at low ionic strength or by heat aggregated IgG (aggIgG) measured by the hydrolysis of acetyl tyrosine ethyl ester (ATEe) by Cls. When serum was mixed with aggIgG, complement was activated and consumed. If lysine was present in the serum containing aggIgG, the activation of comple...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::The fungus metabolite cyclosporin A is a small cyclic peptide acting as a novel antilymphocytic agent. It is effective following either parenteral or oral administration in mice, rats and guinea-pigs. The suppressive effect after short and prolonged treatment on plaque-forming cells, the inhibition of the secondary hu...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract::Paratyphoid vaccine injected between 4 days and 3 hours before injection of labelled immune complexes (125-I-labelled BGG-anti-BGG), inhibits follicular trapping of these complexes in the mouse spleen. Inhibition is maximal when paratyphoid vaccine is given 1 day before, almost no label being found in the spleen folli...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
abstract::We have developed a thyroglobulin-specific haemolytic plaque assay and investigated potential sites of autoantibody synthesis in good and poor responder strains of rats immunized with thyroglobulin and in rats subjected to thymectomy and sub-lethal irradiation which subsequently develop thyroiditis spontaneously. The ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract::Radiation chimaeras, prepared from NIH (rapid-responder) mice or from the F1 progeny of a cross between H-2 compatible B10.G (slow-responder) and NIH mice, were tested for their ability to respond to infection with the intestinal nematode parasite Trichinella spiralis. Mice reconstituted with bone marrow (BM) from NIH...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::The third component of complement (C3) is synthesized and released by cultured human capillary endothelial cells. After the incubation of cells in a methionine-free medium containing 35S-methionine for 48 hr, culture supernatants were immunoprecipitated with anti-human C3 serum. SDS solubilization and 2-ME reduction o...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::The innate immune response provides the initial defence mechanism against infection by other organisms. However, an excessive immune response will cause damage to host tissues. In an attempt to identify microRNAs (miRNAs) that regulate the innate immune response in inflammation and homeostasis, we examined the differe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03374.x
更新日期:2011-05-01 00:00:00
abstract::Mouse lymph node cells cultured with Concanavalin A (Con A) in serum-free medium containing 59Fe-transferrin took up 59Fe more rapidly than cells cultured without Con-A. Uptake of iron commenced rapidly and preceded the onset of DNA synthesis in stimulated cells. Total uptake of transferrin during culture was much low...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::Co-stimulation of highly purified peripheral T lymphocytes from healthy blood donors with the adhesion molecules CD2 and CD28 in association with recombinant interleukin-7 (rIL-7) induced T-cell proliferation, multiple cytokine secretion and IL-2 receptivity. We demonstrated that rIL-7 is as potent as rIL-2 in inducin...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::The intestinal lamina propria (LP) contains antigen-presenting cells with features of dendritic cells and macrophages, collectively referred to as mononuclear phagocytes (MNPs). Association of MNPs with the epithelium is thought to play an important role in multiple facets of intestinal immunity including imprinting M...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12801
更新日期:2017-12-01 00:00:00
abstract::A highly purified preparation of mouse B cells showed greatly decreased incorporation of [6-3H]-thymidine when stimulated with pokeweed Pa-1 mitogen or bacterial lipopolysaccharide compared with mouse splenic lymphocytes. This decreased stimulation was restored by the addition of purified T cells, but not macrophages....
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract::We have previously reported that human granulocyte-macrophage colony-stimulating factor (hGM-CSF) causes a stage-specific inhibition of T-cell receptor (TCR) alphabeta cell development in the thymus of transgenic mice constitutively expressing the hGM-CSF receptor. Since it has been reported that the addition of inter...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01402.x
更新日期:2002-06-01 00:00:00
abstract::Activation of human eosinophils by platelet-activating factor (PAF) involves multiple signal transduction pathways. Among these, protein kinase C has been demonstrated both to mediate respiratory burst and to suppress an alternative pathway of activation of respiratory burst and arachidonic acid metabolism in eosinoph...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00037.x
更新日期:2000-06-01 00:00:00
abstract::The ability of preparations of cobra factor (CoF) to convert C3 via the properdin pathway (anticomplementary activity) has been demonstrated to be unrelated to its ascribed ability to convert a tolerogenic signal into an immunogenic stimulus (adjuvant activity). It has been shown that CoF preparations which have becom...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-03-01 00:00:00
abstract::The gut immune system and its modification by diet have been implicated in the pathogenesis of type 1 diabetes (T1D). Therefore, we investigated gut immune status in non-diabetes-prone LEW.1AR1 and diabetes-prone LEW.1AR1-iddm rats and evaluated the effect of a low antigen, hydrolysed casein (HC)-based diet on gut imm...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12457
更新日期:2015-07-01 00:00:00
abstract::In order to elucidate possible roles of thymic nurse cells (TNC) we isolated them as lympho-epithelial cell complexes from spontaneous thymomas of BUF/Mna rats and characterized them using immuno- and enzyme-histochemical techniques. A remarkable increase in the number of TNC was seen at 8 months of age, immediately b...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Gene ablation studies in mice indicate that lymphotoxin (LT)alpha, LTbeta and LTbetaR are essential for the genesis of lymph nodes (LN), normal structural development of peripheral lymphoid tissues and the differentiation of natural killer (NK) cells. LTbetaR binds to the heterotrimeric cytokines LTalpha1beta2 and LIG...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01586.x
更新日期:2003-03-01 00:00:00