Abstract：
:Mammalian cytokinetic abscission is mediated by the ESCRT membrane fission machinery. While much has been clarified on the topology and kinetics of abscission through high-resolution microscopy, key questions regarding the mechanism of abscission remain open. Here we apply cryogenic soft-X-ray tomography to elucidate new ultrastructural details in the intercellular membrane bridge connecting cells undergoing abscission. In particular, we resolve defined ring-like structures inside the midbody dark zone that have been inaccessible to EM, and identify membrane extrusions at the abscission sites. In cells at late stages of abscission we resolve a complex array of helical spirals, extending the structural information obtained by EM. Our results highlight the advantages of soft-X-ray tomography and emphasize the importance of using complementary approaches for characterizing cellular structures. Notably, by providing new structural data from intact cells we present a realistic view on the topology of abscission and suggest new mechanistic models for ESCRT mediated abscission.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sherman S,Kirchenbuechler D,Nachmias D,Tamir A,Werner S,Elbaum M,Elia Ndoi
10.1038/srep27629subject
Has Abstractpub_date
2016-06-10 00:00:00pages
27629issn
2045-2322pii
srep27629journal_volume
6pub_type
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