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Quantitative evaluation of Oryeongsan and its action on water regulation in renal inner medullary collecting duct cells.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:Oryeongsan (ORS, Wulingsan) has been reported to possess renal protective effects from renal diseases such as diabetes-induced renal damage, and nephrocalcinosis. AIM OF THE STUDY:This study was conducted to evaluate the quantitative analysis and the inhibitory effect of ORS on hypertonic stress-induced water channel and apoptosis in murine inner medullary collecting duct cell line (mIMCD-3). MATERIALS AND METHODS:Chromatographic and NMR spectroscopic analysis were performed and water balance regulation was determined by Western blot, RT-PCR, and immunofluorescnece. RESULTS:Seven active principles (5-hydroxymethylfurfural, alismoxide, methyl(-)trans-cinnamate, adenine, guanosine, adenosine, and ferulic acid) in ORS were isolated and the structures were identified mainly by NMR spectroscopic analysis. In addition, contents of these metabolites in ORS were evaluated by HPLC analysis. Pretreatment with ORS significantly attenuated the hypertonic stress (175mM NaCl)-induced increase in protein levels of AQP2 and apical membrane insertion. ORS also attenuated osmolyte sodium-myo-inositol transporter (SMIT) expression and tonicity-responsive enhancer binding protein (TonEBP) mRNA under hypertonic stress. Those actions of ORS presented the similar effect of PKA inhibitor which AQP2 expression throughout the inhibition of vasopressin-mediated cAMP/PKA signal pathway. On the other hand, pretreatment with ORS attenuated hypertonic stress-induced cell death. Hypertonic stress-induced Bax or caspase-3 expression was decreased by ORS, resulting in anti-apoptotic effect. CONCLUSIONS:The present data suggest that the beneficial effect of ORS in water balance and apoptosis against hypertonic stress of renal collecting ducts.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Lee YJ,Lee SM,Cui X,Yoon JJ,Oh HC,Kim YC,Park MC,Kang DG,Lee HS

doi

10.1016/j.jep.2016.03.030

subject

Has Abstract

pub_date

2016-06-05 00:00:00

pages

310-8

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(16)30135-0

journal_volume

185

pub_type

杂志文章

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Quantitative evaluation of Oryeongsan and its action on water regulation in renal inner medullary collecting duct cells.