当前位置： SCI文献检索 > BMC GENETICS期刊下所有文献 > Schrodinger's scat: a critical review of the currently available tiger (Panthera Tigris) and leopard (Panthera pardus) specific primers in India, and a novel leopard specific primer.

Schrodinger's scat: a critical review of the currently available tiger (Panthera Tigris) and leopard (Panthera pardus) specific primers in India, and a novel leopard specific primer.

Abstract：

BACKGROUND:Non-invasive sampling has opened avenues for the genetic study of elusive species, which has contributed significantly to their conservation. Where field based identity of non-invasive sample is ambiguous (e.g. carnivore scats), it is essential to establish identity of the species through molecular approaches. A cost effective procedure to ascertain species identity is to use species specific primers (SSP) for PCR amplification and subsequent resolution through agarose gel electrophoresis. However, SSPs if ill designed can often cross amplify non-target sympatric species. Herein we report the problem of cross amplification with currently published SSPs, which have been used in several recent scientific articles on tigers (Panthera tigris) and leopards (Panthera pardus) in India. Since these papers form pioneering research on which future work will be based, an early rectification is required so as to not propagate this error further. RESULTS:We conclusively show cross amplification of three of the four SSPs, in sympatric non-target species like tiger SSP amplifying leopard and striped hyena (Hyaena hyaena), and leopard SSP amplifying tiger, lion (Panthera leo persica) and clouded leopard (Neofelis nebulosa), with the same product size. We develop and test a non-cross-amplifying leopard specific primer pair within the mitochondrial cytochrome b region. We also standardize a duplex PCR method to screen tiger and leopard samples simultaneously in one PCR reaction to reduce cost and time. CONCLUSIONS:These findings suggest the importance of an often overlooked preliminary protocol of conclusive identification of species from non-invasive samples. The cross amplification of published primers in conspecifics suggests the need to revisit inferences drawn by earlier work.

journal_name

BMC Genet

journal_title

BMC genetics

authors

Maroju PA,Yadav S,Kolipakam V,Singh S,Qureshi Q,Jhala Y

doi

10.1186/s12863-016-0344-y

subject

Has Abstract

pub_date

2016-02-09 00:00:00

pages

37

issn

1471-2156

pii

10.1186/s12863-016-0344-y

journal_volume

17

pub_type

杂志文章,评审

相关文献

BMC GENETICS文献大全
  • Truncating CHRNG mutations associated with interfamilial variability of the severity of the Escobar variant of multiple pterygium syndrome.

    abstract：BACKGROUND:In humans, muscle-specific nicotinergic acetylcholine receptor (AChR) is a transmembrane protein with five different subunits, coded by CHRNA1, CHRNB, CHRND and CHRNG/CHRNE. The gamma subunit of AChR encoded by CHRNG is expressed during early foetal development, whereas in the adult, the γ subunit is replace...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-016-0382-5

    authors： Kariminejad A,Almadani N,Khoshaeen A,Olsson B,Moslemi AR,Tajsharghi H

    更新日期：2016-05-31 00:00:00

  • A genome-wide scanning and fine mapping study of COGA data.

    abstract：:A thorough genetic mapping study was performed to identify predisposing genes for alcoholism dependence using the Collaborative Study on the Genetics of Alcoholism (COGA) data. The procedure comprised whole-genome linkage and confirmation analyses, single locus and haplotype fine mapping analyses, and gene x environme...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-6-S1-S30

    authors： Yang HC,Chang CC,Lin CY,Chen CL,Lin CY,Fann CS

    更新日期：2005-12-30 00:00:00

  • Evaluation of linkage disequilibrium and its effect on non-parametric multipoint linkage analysis using two high density single-nucleotide polymorphism mapping panels.

    abstract：:Genotype data from the Illumina Linkage III SNP panel (n = 4,720 SNPs) and the Affymetrix 10 k mapping array (n = 11,120 SNPs) were used to test the effects of linkage disequilibrium (LD) between SNPs in a linkage analysis in the Collaborative Study on the Genetics of Alcoholism pedigree collection (143 pedigrees; 1,6...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-6-S1-S85

    authors： Murray SS

    更新日期：2005-12-30 00:00:00

  • Association of ADIPOQ gene with type 2 diabetes and related phenotypes in African American men and women: the Jackson Heart Study.

    abstract：BACKGROUND:African Americans experience disproportionately higher prevalence of type 2 diabetes and related risk factors. Little research has been done on the association of ADIPOQ gene on type 2 diabetes, plasma adiponectin, blood glucose, HOMA-IR and body mass index (BMI) in African Americans. The objective of our re...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-015-0319-4

    authors： Davis SK,Xu R,Gebreab SY,Riestra P,Gaye A,Khan RJ,Wilson JG,Bidulescu A

    更新日期：2015-12-23 00:00:00

  • Contribution of genetic effects to genetic variance components with epistasis and linkage disequilibrium.

    abstract：BACKGROUND:Cockerham genetic models are commonly used in quantitative trait loci (QTL) analysis with a special feature of partitioning genotypic variances into various genetic variance components, while the F(infinity) genetic models are widely used in genetic association studies. Over years, there have been some confu...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-10-52

    authors： Wang T,Zeng ZB

    更新日期：2009-09-04 00:00:00

  • Epidermal growth factor receptor gene mutations in patients with lung adenocarcinoma differ by frequency and type between Uighur and Han ethnic groups in Xinjiang Autonomous Region.

    abstract：BACKGROUND:This study was designed to investigate epidermal growth factor receptor (EGFR) mutation types affecting lung cancer treatment in patients in Xinjiang, China. We detected and analyzed differences in the EGFR mutation points of Uighur and Han patients with lung adenocarcinoma. We examined 181 specimens of lung...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-015-0181-4

    authors： Li X,Wang X,Zhu H,Liu C,Zhou X,Zhao B,Duan H,Yang J,Gu G,Zhan Y,Yuan J,Abuduwaili K,Qionglu S

    更新日期：2015-02-28 00:00:00

  • Canine fibroblast growth factor receptor 3 sequence is conserved across dogs of divergent skeletal size.

    abstract：BACKGROUND:Fibroblast growth factor receptor 3 (FGFR3) is expressed in the growth plate of endochondral bones and serves as a negative regulator of linear bone elongation. Activating mutations severely limit bone growth, resulting in dwarfism, while inactivating mutations significantly enhance bone elongation and overa...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-9-67

    authors： Smith LB,Bannasch DL,Young AE,Grossman DI,Belanger JM,Oberbauer AM

    更新日期：2008-10-21 00:00:00

  • Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology.

    abstract：BACKGROUND:The Drosophila central nervous system (CNS) is a convenient model system for the study of the molecular mechanisms of conserved neurobiological processes. The manipulation of gene activity in specific cell types and subtypes of the Drosophila CNS is frequently achieved by employing the binary Gal4/UAS system...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-020-00895-7

    authors： Ogienko AA,Andreyeva EN,Omelina ES,Oshchepkova AL,Pindyurin AV

    更新日期：2020-10-22 00:00:00

  • AB-QTL analysis reveals new alleles associated to proline accumulation and leaf wilting under drought stress conditions in barley (Hordeum vulgare L.).

    abstract：BACKGROUND:Land plants have evolved several measures to maintain their life against abiotic stresses. The accumulation of proline is the most generalized response of plants under drought, heat or salt stress conditions. It is known as an osmoprotectant which also acts as an instant source of energy during drought recov...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-13-61

    authors： Sayed MA,Schumann H,Pillen K,Naz AA,Léon J

    更新日期：2012-07-20 00:00:00

  • Comparative analyses of genetic trends and prospects for selection against hip and elbow dysplasia in 15 UK dog breeds.

    abstract：BACKGROUND:Hip dysplasia remains one of the most serious hereditary diseases occurring in dogs despite long-standing evaluation schemes designed to aid selection for healthy joints. Many researchers have recommended the use of estimated breeding values (EBV) to improve the rate of genetic progress from selection agains...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-14-16

    authors： Lewis TW,Blott SC,Woolliams JA

    更新日期：2013-03-02 00:00:00

  • A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattle.

    abstract：BACKGROUND:Marbling score (MS) is the major quantitative trait that affects carcass quality in beef cattle. In this study, we examined the association between genetic polymorphisms of the micromolar calcium-activated neutral protease gene (micro-calpain, CAPN1) and carcass traits in Korean cattle (also known as Hanwoo)...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-9-33

    authors： Cheong HS,Yoon DH,Park BL,Kim LH,Bae JS,Namgoong S,Lee HW,Han CS,Kim JO,Cheong IC,Shin HD

    更新日期：2008-04-19 00:00:00

  • Progeny-testing of full-sibs IBD in a SSC2 QTL region highlights epistatic interactions for fatness traits in pigs.

    abstract：BACKGROUND:Many QTL have been detected in pigs, but very few of them have been fine-mapped up to the causal mutation. On SSC2, the IGF2-intron3-G3072A mutation has been described as the causative polymorphism for a QTL underlying muscle mass and backfat deposition, but further studies have demonstrated that at least on...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-12-92

    authors： Tortereau F,Sanchez MP,Fève K,Gilbert H,Iannuccelli N,Billon Y,Milan D,Bidanel JP,Riquet J

    更新日期：2011-10-27 00:00:00

  • High density linkage disequilibrium maps of chromosome 14 in Holstein and Angus cattle.

    abstract：BACKGROUND:Linkage disequilibrium (LD) maps can provide a wealth of information on specific marker-phenotype relationships, especially in areas of the genome where positional candidate genes with similar functions are located. A recently published high resolution radiation hybrid map of bovine chromosome 14 (BTA14) tog...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-9-45

    authors： Marques E,Schnabel RD,Stothard P,Kolbehdari D,Wang Z,Taylor JF,Moore SS

    更新日期：2008-07-08 00:00:00

  • MicroRNA-146a rs2910164 is associated with severe preeclampsia in Black South African women on HAART.

    abstract：BACKGROUND:South African (SA) Black women have a high prevalence of preeclampsia and HIV, both conditions associated with increased inflammation. miR-146a is an inflammatory-associated miR and a common single nucleotide polymorphism (rs2910164) has been associated with several disease conditions. To date, this SNP has ...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-016-0469-z

    authors： Maharaj NR,Ramkaran P,Pillay S,Chuturgoon AA

    更新日期：2017-01-19 00:00:00

  • Microsatellite linkage analysis, single-nucleotide polymorphisms, and haplotype associations with ECB21 in the COGA data.

    abstract：:This study, part of the Genetic Analysis Workshop 14 (GAW14), explored real Collaborative Study on the Genetics of Alcoholism data for linkage and association mapping between genetic polymorphisms (microsatellite and single-nucleotide polymorphisms (SNPs)) and beta (16.5-20 Hz) oscillations of the brain rhythms (ecb21...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-6-S1-S94

    authors： Kraja AT,Borecki IB,Province MA

    更新日期：2005-12-30 00:00:00

  • Genome-enabled predictions for binomial traits in sugar beet populations.

    abstract：BACKGROUND:Genomic information can be used to predict not only continuous but also categorical (e.g. binomial) traits. Several traits of interest in human medicine and agriculture present a discrete distribution of phenotypes (e.g. disease status). Root vigor in sugar beet (B. vulgaris) is an example of binomial trait ...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-15-87

    authors： Biscarini F,Stevanato P,Broccanello C,Stella A,Saccomani M

    更新日期：2014-07-22 00:00:00

  • An adaptive gene-level association test for pedigree data.

    abstract：BACKGROUND:We propose a gene-level association test that accounts for individual relatedness and population structures in pedigree data in the framework of linear mixed models (LMMs). Our method data-adaptively combines the results across a class of score-based tests, only requiring fitting a single null model (under t...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-018-0639-2

    authors： Park JY,Wu C,Pan W

    更新日期：2018-09-17 00:00:00

  • Refining associations between TAS2R38 diplotypes and the 6-n-propylthiouracil (PROP) taste test: findings from the Avon Longitudinal Study of Parents and Children.

    abstract：BACKGROUND:Previous investigations have highlighted the importance of genetic variation in the determination of bitter tasting ability, however have left unaddressed questions as to within group variation in tasting ability or the possibility of genetic prescription of intermediate tasting ability. Our aim was to exami...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-8-51

    authors： Timpson NJ,Heron J,Day IN,Ring SM,Bartoshuk LM,Horwood J,Emmett P,Davey-Smith G

    更新日期：2007-07-28 00:00:00

  • Detection of quantitative trait loci affecting haematological traits in swine via genome scanning.

    abstract：BACKGROUND:Haematological traits, which consist of mainly three components: leukocyte traits, erythrocyte traits and platelet traits, play extremely important role in animal immune function and disease resistance. But knowledge of the genetic background controlling variability of these traits is very limited, especiall...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-11-56

    authors： Gong YF,Lu X,Wang ZP,Hu F,Luo YR,Cai SQ,Qi CM,Li S,Niu XY,Qiu XT,Zeng J,Zhang Q

    更新日期：2010-06-28 00:00:00

  • Genomic characterisation, chromosomal assignment and in vivo localisation of the canine high mobility group A1 (HMGA1) gene.

    abstract：BACKGROUND:The high mobility group A1 proteins (HMGA1a/HMGA1b) are highly conserved between mammalian species and widely described as participating in various cellular processes. By inducing DNA conformation changes the HMGA1 proteins indirectly influence the binding of various transcription factors and therefore effec...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-9-49

    authors： Beuing C,Soller JT,Muth M,Wagner S,Dolf G,Schelling C,Richter A,Willenbrock S,Reimann-Berg N,Winkler S,Nolte I,Bullerdiek J,Murua Escobar H

    更新日期：2008-07-23 00:00:00

  • Exome sequencing of a family with lone, autosomal dominant atrial flutter identifies a rare variation in ABCB4 significantly enriched in cases.

    abstract：BACKGROUND:Lone atrial flutter (AFL) and atrial fibrillation (AF) are common and sometimes consequential cardiac conduction disorders with a strong heritability, as underlined by recent genome-wide association studies that identified genetic modifiers. Follow-up family-based genetic analysis also identified Mendelian t...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-015-0177-0

    authors： Maciąg A,Villa F,Ferrario A,Spinelli CC,Carrizzo A,Malovini A,Torella A,Montenero C,Parisi A,Condorelli G,Vecchione C,Nigro V,Montenero AS,Puca AA

    更新日期：2015-02-11 00:00:00

  • Molecular cytogenetics of valuable Arctic and sub-Arctic pasture grass species from the Aveneae/Poeae tribe complex (Poaceae).

    abstract：BACKGROUND:Grasslands in the Arctic tundra undergo irreversible degradation due to climatic changes and also over-exploitation and depletion of scarce resources. Comprehensive investigations of cytogenomic structures of valuable Arctic and sub-Arctic grassland species is essential for clarifying their genetic peculiari...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-019-0792-2

    authors： Amosova AV,Zoshchuk SA,Rodionov AV,Ghukasyan L,Samatadze TE,Punina EO,Loskutov IG,Yurkevich OY,Muravenko OV

    更新日期：2019-12-04 00:00:00

  • Genetic diversity and population structure of the Sapsaree, a native Korean dog breed.

    abstract：BACKGROUND:The Sapsaree is a breed of dog (Canis familiaris) native to Korea, which became perilously close to extinction in the mid-1980s. However, with systematic genetic conservation and restoration efforts, this breed was rescued from extinction and population sizes have been gradually increasing over the past few ...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-019-0757-5

    authors： Gajaweera C,Kang JM,Lee DH,Lee SH,Kim YK,Wijayananda HI,Kim JJ,Ha JH,Choi BH,Lee SH

    更新日期：2019-08-05 00:00:00

  • Identification and mapping of stable QTL with main and epistasis effect on rice grain yield under upland drought stress.

    abstract：BACKGROUND:Drought is one of the most important abiotic stresses that cause drastic reduction in rice grain yield (GY) in rainfed environments. The identification and introgression of QTL leading to high GY under drought have been advocated to be the preferred breeding strategy to improve drought tolerance of popular r...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-15-63

    authors： Sandhu N,Singh A,Dixit S,Sta Cruz MT,Maturan PC,Jain RK,Kumar A

    更新日期：2014-05-27 00:00:00

  • Mutation screen and association studies in the diacylglycerol O-acyltransferase homolog 2 gene (DGAT2), a positional candidate gene for early onset obesity on chromosome 11q13.

    abstract：BACKGROUND:DGAT2 is a promising candidate gene for obesity because of its function as a key enzyme in fat metabolism and because of its localization on chromosome 11q13, a linkage region for extreme early onset obesity detected in our sample. We performed a mutation screen in 93 extremely obese children and adolescents...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-8-17

    authors： Friedel S,Reichwald K,Scherag A,Brumm H,Wermter AK,Fries HR,Koberwitz K,Wabitsch M,Meitinger T,Platzer M,Biebermann H,Hinney A,Hebebrand J

    更新日期：2007-05-03 00:00:00

  • Abnormal X: autosome ratio, but normal X chromosome inactivation in human triploid cultures.

    abstract：BACKGROUND:X chromosome inactivation (XCI) is that aspect of mammalian dosage compensation that brings about equivalence of X-linked gene expression between females and males by inactivating one of the two X chromosomes (Xi) in normal female cells, leaving them with a single active X (Xa) as in male cells. In cells wit...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-7-41

    authors： Gartler SM,Varadarajan KR,Luo P,Norwood TH,Canfield TK,Hansen RS

    更新日期：2006-07-03 00:00:00

  • Genomic prediction of trait segregation in a progeny population: a case study of Japanese pear (Pyrus pyrifolia).

    abstract：BACKGROUND:In cross breeding, it is important to choose a good parental combination that has high probability of generating offspring with desired characteristics. This study examines a method for predicting the segregation of target traits in a progeny population based on genome-wide markers and phenotype data of pare...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-14-81

    authors： Iwata H,Hayashi T,Terakami S,Takada N,Saito T,Yamamoto T

    更新日期：2013-09-12 00:00:00

  • Whole genome scan reveals the genetic signature of African Ankole cattle breed and potential for higher quality beef.

    abstract：BACKGROUND:Africa is home to numerous cattle breeds whose diversity has been shaped by subtle combinations of human and natural selection. African Sanga cattle are an intermediate type of cattle resulting from interbreeding between Bos taurus and Bos indicus subspecies. Recently, research has asserted the potential of ...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-016-0467-1

    authors： Taye M,Kim J,Yoon SH,Lee W,Hanotte O,Dessie T,Kemp S,Mwai OA,Caetano-Anolles K,Cho S,Oh SJ,Lee HK,Kim H

    更新日期：2017-02-09 00:00:00

  • Fast and accurate haplotype frequency estimation for large haplotype vectors from pooled DNA data.

    abstract：BACKGROUND:Typically, the first phase of a genome wide association study (GWAS) includes genotyping across hundreds of individuals and validation of the most significant SNPs. Allelotyping of pooled genomic DNA is a common approach to reduce the overall cost of the study. Knowledge of haplotype structure can provide ad...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/1471-2156-13-94

    authors： Iliadis A,Anastassiou D,Wang X

    更新日期：2012-10-30 00:00:00

  • Focal exposure of limited lung volumes to high-dose irradiation down-regulated organ development-related functions and up-regulated the immune response in mouse pulmonary tissues.

    abstract：BACKGROUND:Despite the emergence of stereotactic body radiotherapy (SBRT) for treatment of medically inoperable early-stage non-small-cell lung cancer patients, the molecular effects of focal exposure of limited lung volumes to high-dose radiation have not been fully characterized. This study was designed to identify m...

    journal_title：BMC genetics

    pub_type： 杂志文章

    doi：10.1186/s12863-016-0338-9

    authors： Kim BY,Jin H,Lee YJ,Kang GY,Cho J,Lee YS

    更新日期：2016-01-27 00:00:00