Abstract:
:A specific protein profile that accompanies neoplastic transformation in the premalignant airway epithelium could provide an opportunity for early diagnosis of lung cancer. The aim of this study was to screen and identify early candidate biomarkers of non-small cell lung cancer. Thirteen non-small cell lung cancer samples were obtained within 30 minutes after a surgical resection. Laser capture microdissection was performed to enrich the normal lung cell and squamous metaplasia or atypical adenomatous hyperplasia cell populations. The resulting tandem mass spectrum was automatically searched for proteins against International Protein Index (IPI) human protein database using the TurboSEQUEST searching engine. The molecular function and biological processes of identified proteins were determined based on universal bioinformatics tools. The 2 proteins of interest, focal adhesion kinase and C-terminal Src kinase, were validated using Western blot method. A total of 863 proteins were identified by automatically searching the tandem mass spectrum, among which 427 were dysregulated expression in premalignant airway epithelium compared with those of normal lung cells. The 427 proteins were mainly distributed in 24 sorts of cellular components, 22 molecular function, 15 biological processes, and 10 significant perturbations of pathways. The most significant network included 48 genes and was related to energy production, cell cytoskeleton, cell adhesion, metabolism, oxidative stress, and small molecule biochemistry. Focal adhesion kinase and C-terminal Src kinase were significantly overexpressed in premalignant lung lesion cells compared with the normal lung cells in 13 cases. We identified that there were 427 proteins involved in non-small cell lung cancer carcinogenic process and confirmed the key biological pathways in premalignant lung tissue. The significantly upregulated focal adhesion kinase and C-terminal Src kinase could be considered as molecular biomarkers for early diagnosis and prognosis of non-small cell lung cancer.
journal_name
Technol Cancer Res Treatjournal_title
Technology in cancer research & treatmentauthors
Nan Y,Du J,Ma L,Jiang H,Jin F,Yang Sdoi
10.1177/1533034615627391subject
Has Abstractpub_date
2017-02-01 00:00:00pages
66-74issue
1eissn
1533-0346issn
1533-0338pii
1533034615627391journal_volume
16pub_type
杂志文章abstract:PURPOSE:In this study, we aimed to evaluate the prognostic value of fluorodeoxyglucose uptake in the lung parenchyma and the presence of subclinical interstitial lung disease on computed tomography as predictive factors for survival following stereotactic body radiation therapy in patients with stage I non-small cell l...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818794934
更新日期:2018-01-01 00:00:00
abstract::Lung carcinoma is the most frequently diagnosed malignancy in the world, with the incidence increasing through the 20th century. Presentation may be as a tumor mass primarily obstructing the central bronchial lumen, or a mass infiltrating lung tissue. Cryosurgery can be used as a method of palliative treatment for bot...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460400300207
更新日期:2004-04-01 00:00:00
abstract:UNLABELLED:The purpose of this study was to compare the prognostic value of the percentage of positive biopsy cores (PPBC), the percentage of cancer volume (PCV), and the maximum involvement of biopsy cores (MIBC) as a prognostic factor in low- and intermediate-risk patients with clinically localized prostate cancer wh...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrtexpress.2013.600271
更新日期:2014-06-01 00:00:00
abstract::To describe the technological background, the accuracy, and clinical feasibility for single session lung radiosurgery using a real-time robotic system with respiratory tracking. The latest version of image-guided real-time respiratory tracking software (Synchrony, Accuray Incorporated, Sunnyvale, CA) was applied and i...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460700600409
更新日期:2007-08-01 00:00:00
abstract::Numerous beam directions using 3-D conformal techniques can be employed in treating tumors in the posterior fossa, each with characteristic normal tissue exposure along the entrance and exit trajectory. A representative variety of beam configurations were modeled in a modern computer planning system initially with the...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460300200611
更新日期:2003-12-01 00:00:00
abstract::The PIN diode developed by Bhabha Atomic Research Centre (BARC) was modified for its use as a dosimeter in radiation therapy. For this purpose the diode was mounted on a printed circuit board (PCB) and provided with necessary connections so that its response against irradiation can be recorded by a standard radiothera...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500388
更新日期:2014-08-01 00:00:00
abstract::Although an MRI scanner is a single stand-alone modality, different acquisition techniques may be applied to collect structural and functional information, including vascular or angiogenic properties measured by dynamic contrast enhanced MRI (DCE-MRI) and Choline (Cho) metabolism measured by proton MR spectroscopy ima...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:
更新日期:2006-08-01 00:00:00
abstract::One of the most lethal carcinomas is pancreatic cancer. As standard treatment using chemotherapy and radiation has shown limited success, thermal regimens (cryotherapy or heat ablation) are emerging as viable alternatives. Although promising, our understanding of pancreatic cancer response to thermal ablation remains ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034616655658
更新日期:2017-08-01 00:00:00
abstract:PURPOSE:The study was aimed to compare online 6 degree-of-freedom image registrations of TrueBeam cone-beam computed tomography and BrainLab ExacTrac X-ray imaging systems for intracranial radiosurgery. METHODS:Phantom and patient studies were performed on a Varian TrueBeam STx linear accelerator (version 2.5), which ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034616683069
更新日期:2017-06-01 00:00:00
abstract::To quantify the advantages of a 6 degrees of freedom (dof) versus the conventional 3- or 4-dof correction modality for stereotactic body radiation therapy (SBRT) treatments. Eighty-five patients were fitted with 5-7 infra-red passive markers for optical localization. Data, acquired during the treatment, were analyzed ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460800700304
更新日期:2008-06-01 00:00:00
abstract::The inhibitor of growth (ING) family proteins have been defined as candidate tumor suppressors. ING4 as a novel member of ING family has potential tumor-suppressive effects via multiple pathways. However, the therapeutic effect of adenovirus-mediated ING4 (Ad-ING4) gene transfer in human osteosarcoma is still unknown....
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500424
更新日期:2015-10-01 00:00:00
abstract::Histological differentiation is a major pathological criterion indicating the risk of tumor invasion and metastasis in patients with hepatocellular carcinoma. The degree of tumor differentiation is controlled by a complex interacting network of associated proteins. The principal aim of the present study is to identify...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034617732426
更新日期:2017-12-01 00:00:00
abstract::Many conventional chemotherapeutic drugs, as well as DNA for cancer gene therapy, require efficient access to the cell interior to be effective. The cell membrane is a formidable barrier to many of these drugs, including therapeutic DNA constructs. Electropermeabilization (EP, often used synonymously with "electropora...
journal_title:Technology in cancer research & treatment
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1177/153303460200100110
更新日期:2002-02-01 00:00:00
abstract::This study was performed to observe the effect of the combination of nimotuzumab with radiation or gemcitabine-based chemoradiation on antipancreatic cancer cell therapy. Pancreatic cancer cells (PANC-1) were treated with nimotuzumab alone or combined with radiation (2, 4, or 8 Gy), which was either with or without ge...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615585209
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND:Circulating tumor cells (CTCs) hold huge potential for both clinical applications and basic research into the management of cancer, but the relationship between CTC count and cervical cancer prognosis remains unclear. Therefore, research on this topic is urgently required. OBJECTIVE:This study investigated ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820957005
更新日期:2020-01-01 00:00:00
abstract::Hepatocellular carcinoma is the fourth leading cause of cancer-related death worldwide. Depending on the extent of disease and competing comorbidities for mortality, multiple liver-directed therapy options exist for the treatment of hepatocellular carcinoma. Advancements in radiation oncology have led to the emergence...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,评审
doi:10.1177/1533033818790217
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Antibody resistance, both de novo and acquired, is usually related to high risk of recurrence and lower survival rate in gynecologic cancers. Prevention or reversal of the resistance often yields beneficial clinical results. It was reported that anti-human epidermal growth factor receptor 3 monoclonal antibo...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615588422
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:Recent studies have confirmed that immune-associated genes perform a crucial function in recurrence and metastasis of thyroid carcinoma. A reliable immune-related prognostic signature for patients with thyroid cancer is needed. This study constructed a novel immune-related prognostic signature for thyroid ca...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820935860
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:Gene expression profiles from early-onset breast cancer and normal tissues were analyzed to explore the genes and prognostic factors associated with breast cancer. METHODS:GSE109169 and GSE89116 were obtained from the database of Gene Expression Omnibus. We firstly screened the differentially expressed gene...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033819893670
更新日期:2020-01-01 00:00:00
abstract::Non-thermal irreversible electroporation (N-TIRE) has shown promise as an ablative therapy for a variety of soft-tissue neoplasms. Here we describe the therapeutic planning aspects and first clinical application of N-TIRE for the treatment of an inoperable, spontaneous malignant intracranial glioma in a canine patient...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500181
更新日期:2011-02-01 00:00:00
abstract:PURPOSE:To evaluate a method for reducing metal artifacts, arising from dental fillings, on cone-beam computed tomography images. MATERIALS AND METHODS:A projection interpolation algorithm is applied to cone-beam computed tomography images containing metal artifacts from dental fillings. This technique involves identi...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034615618319
更新日期:2016-12-01 00:00:00
abstract::Targeted immunotherapy using dendritic cell vaccine has been employed for the treatment of solid tumors. Topical 5-aminolevulinic acid-mediated photodynamic therapy, an established approach for topical cancers, can induce an effective antitumor immune response. We have previously shown that 5-aminolevulinic acid-media...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033818785275
更新日期:2018-01-01 00:00:00
abstract::Optical mammography is one of several new techniques for breast cancer detection and characterization presently under development for clinical use that provide information other than morphologic, in particular on the biochemical and metabolic state of normal and diseased tissue. In breast tissue, scattering of red to ...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460500400503
更新日期:2005-10-01 00:00:00
abstract::Growing evidence suggests that aberration of the DNA repair pathway significantly contributes to tumorigenesis. Single-nucleotide polymorphisms in DNA repair-related genes such as WRN have been implicated in cancer risk. However, the results of published studies remain inconclusive. Therefore, we performed a meta-anal...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章,meta分析
doi:10.1177/1533034614561359
更新日期:2016-02-01 00:00:00
abstract::A fiber confocal back scattering micro-spectrometer (FCBS) was established, which combined fiber confocal microscopy with light scattering spectroscopy (LSS) for early diagnosis of the cancer cell at cellular level. An adherent monolayer human normal gastric epithelium line GES-1 and a carcinoma cell line NCI-N87 as w...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500222
更新日期:2011-10-01 00:00:00
abstract::Colorectal cancer is one of the most common cancers in the world. Protein phosphatase magnesium-dependent 1 d (PPM1D) is aberrantly upregulated in many human carcinoma cells, and recent research has suggested that it could be a potential therapeutic target of cancer. However, the function of PPM1D in colorectal carcin...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.7785/tcrt.2012.500349
更新日期:2013-12-01 00:00:00
abstract::The microRNA-200 (miR-200) family has been reported to be vital for the inhibition of epithelial-to-mesenchymal transition (EMT) in tumor cells. The miR-200 family represents a complex multi-factorial regulatory network which has not been well described in breast cancer. This study aimed to clarify the underlying regu...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820979672
更新日期:2020-01-01 00:00:00
abstract::The study of the effectiveness of cryotherapy as a curative treatment for prostate cancer has often relied on the use of either in vitro cell culture monolayers or animal models. While the data gleaned from these studies have been valuable, each model has inherent limitations. In order to bridge the gap between in vit...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/153303460700600204
更新日期:2007-04-01 00:00:00
abstract::eLiposomes encapsulate a perfluorocarbon nanoemulsion droplet inside a liposome. Ultrasound is then used as a trigger mechanism to vaporize the perfluorocarbon, break the liposome, and release the desired drug to the tumor tissue. The purpose of this research is to show that eLiposomes synthesized using perfluoropenta...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533034614551480
更新日期:2015-08-01 00:00:00
abstract:INTRODUCTION:Osteosarcoma is the most common primary tumor of bone, although some molecular markers have been identified, the detailed molecular mechanisms underlying osteosarcoma are currently not fully understood. In the present study, we attempted to identify the potential key genes and pathways in osteosarcoma usin...
journal_title:Technology in cancer research & treatment
pub_type: 杂志文章
doi:10.1177/1533033820965605
更新日期:2020-01-01 00:00:00