Esophageal squamous cell carcinoma cell proliferation induced by exposure to low concentration of cigarette smoke extract is mediated via targeting miR-101-3p/COX-2 pathway.

Abstract:

:Cigarette smoke has been implicated as a major risk factor for esophageal squamous cell carcinoma (ESCC). Several lines of evidence have suggested that the promoting effect of cigarette smoking extract (CSE) on ESCC is mediated by upregulation of cyclooxygenase-2 (COX-2) expression. Yet, the underlying molecular and cellular mechanisms of how CSE stimulates COX-2 expression and facilitates ESCC development are largely unknown. In the present study, we revealed microRNA (miR)-101-3p expression was downregulated upon exposure to low concentration of CSE in Eca109 cancer cells, and suppression of miR-101-3p was required for low CSE-induced cell proliferation, presenting as overexpression of miR-101-3p reversing CSE stimulated cancer cell growth. Luciferase assay revealed that COX-2 was a direct target for miR-101-3p and overexpression of miR-101-3p decreased cellular COX-2 protein expression. Furthermore, we found that COX-2 inhibitor and knockdown of COX-2 by siRNA interference could abolish CSE-induced cell proliferation, indicating that promotion of cancer cell proliferation by low concentration of CSE was dependent on COX-2 activity. Finally, downregulation of miR-101-3p expression and upregulation of COX-2 was found in ESCC specimens from patients with smoking history. Taken together, our findings revealed a new post-transcriptional mechanism by which CSE regulated COX-2 expression to favor cancer cell proliferation, suggesting miR-101-3p as a potential biomarker and therapeutic target for smoke-related ESCC.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Gong J,Chu Y,Xu M,Huo J,Lv L

doi

10.3892/or.2015.4379

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

463-71

issue

1

eissn

1021-335X

issn

1791-2431

journal_volume

35

pub_type

杂志文章
  • Modifying effect of tuna orbital oil rich in docosahexaenoic acid and vitamin D3 on azoxymethane-induced colonic aberrant crypt foci in rats.

    abstract::The modifying effect of dietary tuna (Thunnus thynnus orientalis) orbital oil rich in docosahexaenoic acid (DHA) and vitamin D3 (VD3) on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) was investigated in male F344 rats. Animals were given three weekly subcutaneous injections of AOM (15...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.7.5.1069

    authors: Kohno H,Yamaguchi N,Ohdoi C,Nakajima S,Odashima S,Tanaka T

    更新日期:2000-09-01 00:00:00

  • MicroRNA-195-5p is a potential diagnostic and therapeutic target for breast cancer.

    abstract::MicroRNAs (miRNAs) are a class of highly conserved, small endogenous single-strand non-coding RNAs. They are aberrantly expressed in the circulation and tissue of patients with cancer. Therefore, it has been suggested that they may act as key regulators of carcinogenesis. The aim of the present study was to examine th...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.2971

    authors: Luo Q,Wei C,Li X,Li J,Chen L,Huang Y,Song H,Li D,Fang L

    更新日期:2014-03-01 00:00:00

  • The role of targeting kinase activity by natural products in cancer chemoprevention and chemotherapy (Review).

    abstract::The WHO clearly identifies tumors as a curable or a chronic disease. The use of natural agents in cancer prevention and therapy is currently playing an important role. Our laboratory has been investigating various natural phenolic compounds, including grifolin, neoalbaconol and epigallocatechin-3-gallate (EGCG). In th...

    journal_title:Oncology reports

    pub_type: 杂志文章,评审

    doi:10.3892/or.2015.4029

    authors: Luo X,Yu X,Liu S,Deng Q,Liu X,Peng S,Li H,Liu J,Cao Y

    更新日期:2015-08-01 00:00:00

  • Expression of transglutaminases in human breast cancer and their possible clinical significance.

    abstract::Implicated in several physiologic processes, including tumour-host, some transglutaminases (TGase) have been noted to play a regulatory role in the extracellular matrix in cell adhesion and migration of cancer cells. This study sought to determine the level of expression of TGases and their possible clinical significa...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Jiang WG,Ablin R,Douglas-Jones A,Mansel RE

    更新日期:2003-11-01 00:00:00

  • Knockdown of Bmi1 inhibits the stemness properties and tumorigenicity of human bladder cancer stem cell-like side population cells.

    abstract::B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi1) is directly involved in cell growth, proliferation and self-renewal of cancer stem cells (CSCs). The aim of the present study was to assess the role of Bmi1 in the maintenance of stemness properties and tumorigenicity of human bladder CSC-like side ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2919

    authors: Zhu D,Wan X,Huang H,Chen X,Liang W,Zhao F,Lin T,Han J,Xie W

    更新日期:2014-02-01 00:00:00

  • Pancreatic tumor mass in a xenograft mouse model is decreased by treatment with therapeutic stem cells following introduction of therapeutic genes.

    abstract::Pancreatic cancer is the fourth most common cause of cancer-related mortality. In the present study, we employed 2 types of therapeutic stem cells expressing cytosine deaminase (CD) with or without human interferon-β (IFN‑β), HB1.F3.CD and HB1.F3.CD.IFN-β cells, respectively, to selectively treat pancreatic cancer. Th...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2564

    authors: Kim DJ,Yi BR,Lee HR,Kim SU,Choi KC

    更新日期:2013-09-01 00:00:00

  • Molecular genetic analyses of the TMPRSS2-ERG and TMPRSS2-ETV1 gene fusions in 50 cases of prostate cancer.

    abstract::Recently, gene fusions between the androgen responsive gene TMPRSS2 and members of the ETS-family of DNA-binding transcription factor genes were found in prostate cancer. Recurrent fusions were identified between the 5'-noncoding region of TMPRSS2 and ERG, or less frequently ETV1 or ETV4, resulting in overexpression o...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Winnes M,Lissbrant E,Damber JE,Stenman G

    更新日期:2007-05-01 00:00:00

  • miR-34a inhibits the metastasis of osteosarcoma cells by repressing the expression of CD44.

    abstract::Osteosarcoma is the most common type of malignant bone tumor in children and adolescents and approximately 30% of patients develop lung metastasis, which is the leading cause of mortality. In this study, we investigated the role of miR-34a in the invasion and metastasis of osteosarc...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2234

    authors: Zhao H,Ma B,Wang Y,Han T,Zheng L,Sun C,Liu T,Zhang Y,Qiu X,Fan Q

    更新日期:2013-03-01 00:00:00

  • Antitumor effects of IDN5109 on head and neck squamous cell carcinoma.

    abstract::Taxanes, a new class of antitumor drugs, are effective against a large number of human tumors, although there are problems with drug resistance. The novel taxane, IDN5109, is characterized by its high tolerability, antitumor efficacy, ability to overcome multidrug resistance, and oral bioavailabilty. We investigated t...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Sano D,Matsuda H,Ishiguro Y,Nishimura G,Kawakami M,Tsukuda M

    更新日期:2006-02-01 00:00:00

  • Improved sensitivity and specificity for prostate cancer diagnosis based on the urine PCA3/PSA ratio acquired by sequence‑specific RNA capture.

    abstract::Prostate cancer antigen 3 (PCA3) is a non-coding RNA fragment that is overexpressed in prostate cancer cells. However, the clinical applications of PCA3 are highly limited due to the instability of RNA and the lack of reliable and efficient RNA extraction and purification methods. Thus, in the present study, we compar...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4266

    authors: Zheng K,Dou Y,He L,Li H,Zhang Z,Chen Y,Ye A,Liu W,Kong L

    更新日期:2015-11-01 00:00:00

  • Polymorphisms in base excision repair genes and thyroid cancer risk.

    abstract::Thyroid cancer (TC) is the most frequent endocrine malignancy, accounting however for only 1-2% of all human cancers, and the best-established risk factor for TC is radiation exposure, particularly during childhood. Since the BER pathway seems to play an important role in the repair of DNA damage induced by IR and oth...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1975

    authors: Santos LS,Branco SC,Silva SN,Azevedo AP,Gil OM,Manita I,Ferreira TC,Limbert E,Rueff J,Gaspar JF

    更新日期:2012-11-01 00:00:00

  • Evaluation of diethylnitrosamine- or hepatitis B virus X gene-induced hepatocellular carcinoma with 18F-FDG PET/CT: a preclinical study.

    abstract::The aim of this study was to evaluate whether the development of hepatocellular carcinoma (HCC) in murine models resembles tumor progression in humans, using non‑invasive molecular imaging methods. Murine HCC models were generated by treating mice with diethylnitrosamine (DEN) or by the transgenic expression of hepati...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3575

    authors: Park JH,Kang JH,Lee YJ,Kim KI,Lee TS,Kim KM,Park JA,Ko YO,Yu DY,Nahm SS,Jeon TJ,Park YS,Lim SM

    更新日期:2015-01-01 00:00:00

  • Withaferin A inhibits matrix metalloproteinase-9 activity by suppressing the Akt signaling pathway.

    abstract::Withaferin A (Wit A), a steroidal lactone isolated from Withania somnifera, exhibits anti-inflammatory, immuno-modulatory and anti-angiogenic properties and antitumor activities. In the present study, we investigated the effects of Wit A on protease-mediated invasiveness of the human metastatic cancer cell lines Caski...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2487

    authors: Lee DH,Lim IH,Sung EG,Kim JY,Song IH,Park YK,Lee TJ

    更新日期:2013-08-01 00:00:00

  • Human umbilical vein endothelial cell vaccine suppresses the angiogenesis of esophageal squamous cell carcinoma in a humanized mouse model.

    abstract::The antitumor effect of the human umbilical vein endothelial cell (HUVEC) vaccine has been well documented; however, its anti‑angiogenic effects on human esophageal squamous cell carcinoma (ESCC) have yet to be studied. In the present study, a 'humanized' mouse model was established by transplanting NOD/SCID mice with...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6653

    authors: Liu H,Zhao J,Yang Y,Jin G,Zhang X,Wang D,Xie C,Liu K,Chen X,Li X,Tian F,Dong Z,Lu J

    更新日期:2018-11-01 00:00:00

  • NSPc1 polycomb protein complex binds and cross‑talks to lncRNAs in glioma H4 cells.

    abstract::Recently, emerging evidence shows that a number of long non‑coding RNAs (lncRNAs) recruit polycomb group (PcG) proteins to specific chromatin loci to silence relevant gene expression. In the present study, we provided evidence that lncRNA candidates, selected by bioinformatic analysis and nervous system polycomb 1 (NS...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7000

    authors: Wang Y,Liang Z,Li H,Tao J,Sun Y,Gong Y

    更新日期:2019-04-01 00:00:00

  • Paclitaxel enhances tumoricidal potential of TRAIL via inhibition of MAPK in resistant gastric cancer cells.

    abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) holds promise for cancer therapy due to its unique capacity to selectively trigger apoptosis in cancer cells. However, TRAIL therapy is greatly hampered by its resistance. A preclinical successful strategy is to identify combination treatments that sensit...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4666

    authors: Li L,Wen XZ,Bu ZD,Cheng XJ,Xing XF,Wang XH,Zhang LH,Guo T,Du H,Hu Y,Fan B,Ji JF

    更新日期:2016-05-01 00:00:00

  • Genome-wide profiling of long non-coding RNA expression patterns in the EGFR-TKI resistance of lung adenocarcinoma by microarray.

    abstract::Mutations in the epidermal growth factor receptor (EGFR) make lung adenocarcinoma cells sensitive to EGFR tyrosine kinase inhibitors (TKIs). Long-term cancer therapy may cause the occurrence of acquired resistance to EGFR TKIs. Long non-coding RNAs (lncRNAs) play important roles in tumor formation, tumor metastasis an...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4758

    authors: Wu Y,Yu DD,Hu Y,Yan D,Chen X,Cao HX,Yu SR,Wang Z,Feng JF

    更新日期:2016-06-01 00:00:00

  • CX3CL1 increases invasiveness and metastasis by promoting epithelial-to-mesenchymal transition through the TACE/TGF-α/EGFR pathway in hypoxic androgen-independent prostate cancer cells.

    abstract::Epithelial-to-mesenchymal transition (EMT) endows cancer cells with enhanced invasive and metastatic potential during cancer progression. Fractalkine, also known as chemokine (C-X3-C motif) ligand 1 (CX3CL1), the only member recognized so far that belongs to the CX3C chemokine subfamily, was reported to participate in...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4470

    authors: Tang J,Xiao L,Cui R,Li D,Zheng X,Zhu L,Sun H,Pan Y,Du Y,Yu X

    更新日期:2016-02-01 00:00:00

  • High-level expression of podoplanin in benign and malignant soft tissue tumors: immunohistochemical and quantitative real-time RT-PCR analysis.

    abstract::Podoplanin is a 38 kDa mucin-type transmembrane glycoprotein that was first identified in rat glomerular epithelial cells (podocytes). It is expressed in normal lymphatic endothelium, but is absent from vascular endothelial cells. D2-40 is a commercially available mouse monoclonal antibody which binds to an epitope on...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1141

    authors: Xu Y,Ogose A,Kawashima H,Hotta T,Ariizumi T,Li G,Umezu H,Endo N

    更新日期:2011-03-01 00:00:00

  • Fat accumulation in adipose tissues as a risk factor for the development of endometrial cancer.

    abstract::Fat accumulation in adipose tissues is a risk factor for the development of human cancers. However, there are no studies describing the fat accumulation in adipose tissue or its distribution in human endometrial cancer. We first examined fat accumulation in adipose tissues separately on CT images of 122 patients with ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1259

    authors: Nakamura K,Hongo A,Kodama J,Hiramatsu Y

    更新日期:2011-07-01 00:00:00

  • Ets-1 is implicated in the regulation of androgen co-regulator FHL2 and reveals specificity for migration, but not invasion, of PC3 prostate cancer cells.

    abstract::Different members of the Ets-family of transcription factors are involved in TMPRSS-2-Ets translocations frequently found in human prostate cancers. We previously reported that Ets-1, which is the prototype of Ets-family members, promotes both migration and invasion of melanoma, Hela and glioma cells. Here, we examine...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1156

    authors: Shaikhibrahim Z,Langer B,Lindstrot A,Florin A,Bosserhoff A,Buettner R,Wernert N

    更新日期:2011-04-01 00:00:00

  • Induction and mechanism of HeLa cell apoptosis by 9-oxo‑10, 11-dehydroageraphorone from Eupatorium adenophorum.

    abstract::9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum. The aim of the present study was to examine the induction and mechanism of HeLa cell apoptosis by euptox A. The apoptosis‑inducing effect of the euptox A on HeLa cells was examined by MTT assay. The un...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.3778

    authors: Liao F,Hu Y,Wu L,Tan H,Luo B,He Y,Qiao Y,Mo Q,Wang Y,Zuo Z,Deng J,Wei Y

    更新日期:2015-04-01 00:00:00

  • Effective antitumor immunity against murine gliomas using dendritic cells transduced with hTERTC27 recombinant adenovirus.

    abstract::hTERTC27, a 27-kDa hTERT C-terminal polypeptide has been demonstrated to cause hTERT-positive HeLa cell apoptosis and inhibits the growth of mouse melanoma. hTERTC27 has been associated with telomere dysfunction, regulation of gene-regulated apoptosis, the cell cycle and activation of natural killer (NK) cells, but it...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1619

    authors: Gong HX,He L,Li XP,Wang YD,Li Y,Huang JJ,Wang Z,Xie D,Kung HF,Peng Y

    更新日期:2012-04-01 00:00:00

  • Elevated L1CAM expression in precursor lesions and primary and metastastic tissues of pancreatic ductal adenocarcinoma.

    abstract::The promigratory molecule L1CAM is overexpressed in various tumors, often representing an unfavorable prognostic marker. Recently, we identified L1CAM expression in pancreatic ductal adenocarcinoma (PDAC) cells accounting for chemoresistance and increased cell migration. Thus, the present study aims at further elucida...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2010.909

    authors: Bergmann F,Wandschneider F,Sipos B,Moldenhauer G,Schniewind B,Welsch T,Schirrmacher P,Klöppel G,Altevogt P,Schäfer H,Sebens Müerköster S

    更新日期:2010-10-01 00:00:00

  • Optimal subset of signature miRNAs consisting of 7 miRNAs that can serve as a novel diagnostic and prognostic predictor for the progression of cervical cancer.

    abstract::Cervical cancer is the second most commonly diagnosed cancer in women. Novel prognostic biomarkers are required to predict the progression of cervical cancer. Cervical cancer expression data were obtained from The Cancer Genome Atlas (TCGA) database. MicroRNAs (miRNAs) significantly differentially expressed between ea...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7097

    authors: Shi C,Yang Y,Zhang L,Zhang T,Yu J,Qin S,Gao Y

    更新日期:2019-06-01 00:00:00

  • NRF2 is overexpressed in ovarian epithelial carcinoma and is regulated by gonadotrophin and sex-steroid hormones.

    abstract::Aberrant nuclear factor-erythroid 2 (NRF2) expression correlates with tumor development. We investigated NRF2 expression in ovarian epithelial carcinoma (OEC), aiming to identify associations with clinicopathological factors, hormones and induced reactive oxygen species (ROS). Immun...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1700

    authors: Liao H,Zhou Q,Zhang Z,Wang Q,Sun Y,Yi X,Feng Y

    更新日期:2012-06-01 00:00:00

  • miR-3619-5p inhibits prostate cancer cell growth by activating CDKN1A expression.

    abstract::Recent studies have shown that miRNAs have potent abilities to activate gene expression by targeting promoter elements, a phenomenon known as RNA activation (RNAa). In the present study, we identified a new endogenous miR-3619-5p which was decreased in prostate cancer tissues and cells compared to corresponding normal...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.5250

    authors: Li S,Wang C,Yu X,Wu H,Hu J,Wang S,Ye Z

    更新日期:2017-01-01 00:00:00

  • Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation.

    abstract::The activation of receptor tyrosine kinases (RTKs) results in cellular effects including cell proliferation, survival, migration and invasion; RTKs also play an important role in tumourigenesis. It has been reported that EGFR signalling controls the migration of oral squamous cell carcinoma (OSCC) SAS and HSC3 cells b...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5587

    authors: Yasui H,Ohnishi Y,Nakajima M,Nozaki M

    更新日期:2017-06-01 00:00:00

  • Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is associated with a poor prognosis.

    abstract::The aim of the present study was to investigate the expression, function and underlying molecular mechanism of the long non‑coding (lnc) RNA RP1‑163G9.1 in patients with gastric adenocarcinoma (GA). The expression levels of lncRNA RP1‑163G9.1 were determined in 112 paired clinical GA tissues by reverse transcription‑q...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7127

    authors: Sun B,Dang Y,Zhang F,Li K,Ouyang X,Wang K,Huang Q

    更新日期:2019-06-01 00:00:00

  • Biological comparison of ovarian cancer resistant cell lines to cisplatin and Taxol by two different administrations.

    abstract::Drug resistance is one of the major obstacles to chemotherapy of ovarian cancer. Studies with cell lines can serve as an initial screen for agents that might modulate drug resistance. To establish more appropriate models of drug resistance and explore whether the differences exist in the different drug resistant subli...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Yan XD,Li M,Yuan Y,Mao N,Pan LY

    更新日期:2007-05-01 00:00:00