Esophageal squamous cell carcinoma cell proliferation induced by exposure to low concentration of cigarette smoke extract is mediated via targeting miR-101-3p/COX-2 pathway.

Abstract:

:Cigarette smoke has been implicated as a major risk factor for esophageal squamous cell carcinoma (ESCC). Several lines of evidence have suggested that the promoting effect of cigarette smoking extract (CSE) on ESCC is mediated by upregulation of cyclooxygenase-2 (COX-2) expression. Yet, the underlying molecular and cellular mechanisms of how CSE stimulates COX-2 expression and facilitates ESCC development are largely unknown. In the present study, we revealed microRNA (miR)-101-3p expression was downregulated upon exposure to low concentration of CSE in Eca109 cancer cells, and suppression of miR-101-3p was required for low CSE-induced cell proliferation, presenting as overexpression of miR-101-3p reversing CSE stimulated cancer cell growth. Luciferase assay revealed that COX-2 was a direct target for miR-101-3p and overexpression of miR-101-3p decreased cellular COX-2 protein expression. Furthermore, we found that COX-2 inhibitor and knockdown of COX-2 by siRNA interference could abolish CSE-induced cell proliferation, indicating that promotion of cancer cell proliferation by low concentration of CSE was dependent on COX-2 activity. Finally, downregulation of miR-101-3p expression and upregulation of COX-2 was found in ESCC specimens from patients with smoking history. Taken together, our findings revealed a new post-transcriptional mechanism by which CSE regulated COX-2 expression to favor cancer cell proliferation, suggesting miR-101-3p as a potential biomarker and therapeutic target for smoke-related ESCC.

journal_name

Oncol Rep

journal_title

Oncology reports

authors

Gong J,Chu Y,Xu M,Huo J,Lv L

doi

10.3892/or.2015.4379

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

463-71

issue

1

eissn

1021-335X

issn

1791-2431

journal_volume

35

pub_type

杂志文章
  • Loss of ONECUT1 expression in human pancreatic cancer cells.

    abstract::ONECUT1 (HNF-6) is the prototype of a new class of homeodomain transcription factors, that controls the development of pancreatic ducts during mouse development. In the present study, the role of ONECUT1 and its targeted genes TCF2, PKHD1 and CYS1 was analyzed in human pancreatic ductal adenocarcinoma (PDAC). mRNA lev...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Jiang X,Zhang W,Kayed H,Zheng P,Giese NA,Friess H,Kleeff J

    更新日期:2008-01-01 00:00:00

  • MicroRNA-411 inhibits malignant biological behaviours of colorectal cancer cells by directly targeting PIK3R3.

    abstract::Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer-related mortality worldwide. Aberrant expression of miRNAs play important roles in the development and progression of various types of cancers by modulating oncogenic and tumour-suppressor pathways. Therefore, exploring the ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.6135

    authors: Zhao J,Xu J,Zhang R

    更新日期:2018-02-01 00:00:00

  • Restoration of connexin 43 by Bowman-Birk protease inhibitor in M5076 bearing mice.

    abstract::The present study was designed to investigate the effects of Bowman-Birk inhibitor (BBI) on up-regulation of connexin (Cx) expression to estimate BBI's tumor-suppressor effect in mice with M5076 ovarian sarcoma. The relative tumor weight (p<0.05, r(2)=0.301) and proliferating cell nuclear antigen (PCNA, p<0.01, r(2)=0...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Suzuki K,Yano T,Sadzuka Y,Sugiyama T,Seki T,Asano R

    更新日期:2005-06-01 00:00:00

  • Triptolide-induced cell cycle arrest and apoptosis in human renal cell carcinoma cells.

    abstract::Renal cell carcinoma (RCC) is the most frequent type of renal-originated malignancy. Although nephrectomy is successfully used to save the lives of patients with localized RCC, treatment of advanced and other refractory RCCs is poor and still inadequate. Here, we show that triptolide, a small molecule and a well-known...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1158

    authors: Li J,Zhu W,Leng T,Shu M,Huang Y,Xu D,Qiu P,Su X,Yan G

    更新日期:2011-04-01 00:00:00

  • Use of CA15‑3 for screening breast cancer: An antibody‑lectin sandwich assay for detecting glycosylation of CA15‑3 in sera.

    abstract::Elevated serum CA15‑3 assessed by enzyme‑linked immunosorbent assay (ELISA) has been considered a diagnostic marker of breast cancer. However, accumulating data indicate that the current ELISA system for detecting CA15‑3, which targets the peptide backbone of CA15‑3, is not sufficiently sensitive to detect early or lo...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6433

    authors: Choi JW,Moon BI,Lee JW,Kim HJ,Jin Y,Kim HJ

    更新日期:2018-07-01 00:00:00

  • Autophagy-mediated HMGB1 release promotes gastric cancer cell survival via RAGE activation of extracellular signal-regulated kinases 1/2.

    abstract::High mobility group box-B1 (HMGB1), an autophagy activator, is crucial in tumorigenesis. However, its extracellular role and signaling in gastric cancer remain unclear. Samples were collected from gastric cancer patients and healthy controls. Immunohistochemistry and immunocytochemistry were used to determine the loca...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.3782

    authors: Zhang QY,Wu LQ,Zhang T,Han YF,Lin X

    更新日期:2015-04-01 00:00:00

  • In silico analysis identified miRNA‑based therapeutic agents against glioblastoma multiforme.

    abstract::MicroRNAs (miRNAs or miRs) contribute to the development of various malignant neoplasms, including glioblastoma multiforme (GBM). The present study aimed to explore the pathogenesis of GBM and to identify latent therapeutic agents for patients with GBM, based on an in silico analysis. Gene chips that provide miRNA exp...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7022

    authors: Xiong DD,Xu WQ,He RQ,Dang YW,Chen G,Luo DZ

    更新日期:2019-04-01 00:00:00

  • Possible mechanism of growth inhibition by Scutellaria baicalensis in an estrogen-responsive mouse tumor cell line.

    abstract::We have studied the effects of Saiboku-to, a traditional Chinese medicine having suppressive activities for leukotriene production and release, on the proliferation of the estrogen-responsive mouse Leydig tumor cell line B-1F. In our previous reports, it is shown that Saiboku-to promotes, but Scutellaria baicalensis, ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1196

    authors: Murashima T,Katayama H,Shojiro K,Nishizawa Y

    更新日期:2011-05-01 00:00:00

  • Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligands, MICA/B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity.

    abstract::MHC class I-related chain molecules A and B (MICA and B) expressed on the cell-surface of tumor cells are ligands for an activating receptor, NKG2D, expressed on natural killer (NK) cells and stimulate the NK cell-mediated cytotoxicity. On the other hand, the soluble form of MICA and B produced by proteolytic cleavage...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or_00001026

    authors: Yamanegi K,Yamane J,Kobayashi K,Kato-Kogoe N,Ohyama H,Nakasho K,Yamada N,Hata M,Nishioka T,Fukunaga S,Futani H,Okamura H,Terada N

    更新日期:2010-12-01 00:00:00

  • Triple-negative breast cancer: New therapeutic options via signalling transduction cascades.

    abstract::Triple-negative breast cancer is a highly aggressive type of mammalian carcinoma. It is defined by a rather weak expression of estrogen-, progesterone- and Her2-receptor, and is thus difficult to treat, resulting in low disease-free and overall survival rates of the affected patients. Hence it is important to find new...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5512

    authors: Andergassen U,Kölbl AC,Mumm JN,Mahner S,Jeschke U

    更新日期:2017-05-01 00:00:00

  • Latent membrane protein 1 mediates the resistance of nasopharyngeal carcinoma cells to TRAIL-induced apoptosis by activation of the PI3K/Akt signaling pathway.

    abstract::The 5-year survival rate of nasopharyngeal carcinoma (NPC) is still disappointing despite the much improved technologies in its treatment. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) can selectively induce apoptosis in most tumor cells while sparing normal cells....

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1423

    authors: Li SS,Yang S,Wang S,Yang XM,Tang QL,Wang SH

    更新日期:2011-12-01 00:00:00

  • The function of miRNAs in hepatocarcinogenesis induced by hepatitis B virus X protein (Review).

    abstract::MicroRNAs are short RNAs that play a crucial role in all biological processes through post-transcriptional regulation for protein-coding genes and inducing mRNA degradation. Hepatitis B virus infection has been considered as a major risk factor in hepatocellular carcinoma, further research indicates that hepatitis B v...

    journal_title:Oncology reports

    pub_type: 杂志文章,评审

    doi:10.3892/or.2017.5716

    authors: Wang Z,Wu Z,Huang P

    更新日期:2017-08-01 00:00:00

  • Tropomodulin 3 promotes liver cancer progression by activating the MAPK/ERK signaling pathway.

    abstract::Tropomodulin 3 (TMOD3) is a member of the pointed‑end capping protein family that contributes to invasion and metastasis in several types of malignancies. TMOD3 has been found to be crucial for membranous skeleton and embryonic development; however, little is known regarding the role of TMOD3 in liver cancer progressi...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2019.7052

    authors: Jin C,Chen Z,Shi W,Lian Q

    更新日期:2019-05-01 00:00:00

  • Loss of the metastasis suppressor gene KiSS1 is associated with lymph node metastasis and poor prognosis in human colorectal cancer.

    abstract::Cancer research is currently focused on blocking the metastatic process at its early steps. Some particularly attractive targets are metastasis suppressor genes, which control cancer cell dissemination. The aim of this study was to clarify the relationship between the expression of KiSS1, a metastasis suppressor gene,...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2558

    authors: Okugawa Y,Inoue Y,Tanaka K,Toiyama Y,Shimura T,Okigami M,Kawamoto A,Hiro J,Saigusa S,Mohri Y,Uchida K,Kusunoki M

    更新日期:2013-09-01 00:00:00

  • Downregulation of occludin affects the proliferation, apoptosis and metastatic properties of human lung carcinoma.

    abstract::Lung cancer is the most frequent and deadliest cancer in the world, especially in China. However, the molecular mechanisms involved in lung cancer remain unclear. Occludin (OCLN), one of the first identified tight junction proteins, has been revealed to be a necessary integral protein for tight junction structure and ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6408

    authors: Wang M,Liu Y,Qian X,Wei N,Tang Y,Yang J

    更新日期:2018-07-01 00:00:00

  • A peptide derived from alpha-fetoprotein inhibits the proliferation induced by estradiol in mammary tumor cells in culture.

    abstract::This study was aimed to obtain additional information on the activity of a cyclized 9-amino acid peptide (cP) containing the active site of alpha fetoprotein, which inhibits the estrogen-stimulated proliferation of tumor cells in culture and of xenografts in immunodeficient mice. Breast cancer cells cultured in the pr...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Sierralta WD,Epuñan MJ,Reyes JM,Valladares LE,Andersen TT,Bennett JA,Jacobson HI,Pino AM

    更新日期:2008-01-01 00:00:00

  • Involvement of ROS in the inhibitory effect of thermotherapy combined with chemotherapy on A549 human lung adenocarcinoma cell growth through the Akt pathway.

    abstract::Mechanisms of synergistic effect of thermotherapy and chemotherapy on human lung adenocarcinoma cell growth are unclear. The purpose of this study was to investigate these effects and explore the function of ROS, Akt and caspase-3 in relation to these. A549 cells were subjected to different thermochemotherapy treatmen...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1954

    authors: Wang L,Liu X,Wu Y,Wu W,Wu Y

    更新日期:2012-10-01 00:00:00

  • Nuclear EpICD expression and its role in hepatocellular carcinoma.

    abstract::Regulated intramembrane proteolysis of epithelial cell adhesion molecule (EpCAM) results in shedding of the extracellular domain (EpEX) and release of the intra-cellular domain (EpICD) into the cytoplasm. Released EpICD associates with FHL2, β-catenin and Lef-1 to form a nuclear complex and triggers oncogenic signalin...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2016.4789

    authors: Park SY,Bae JS,Cha EJ,Chu HH,Sohn JS,Moon WS

    更新日期:2016-07-01 00:00:00

  • G31P, CXCR1/2 inhibitor, with cisplatin inhibits the growth of mice hepatocellular carcinoma and mitigates high‑dose cisplatin-induced nephrotoxicity.

    abstract::Cisplatin (DDP), a cytotoxic antitumor drug, functions in a dose-dependent manner. However, the pursuit for high‑dose therapeutic effects leads to more serious side effects including kidney toxicity. Nephrotoxicity caused due to endothelial cell dysfunction and neutrophils infiltration in kidneys. Interleukin-8 (IL-8)...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2014.3659

    authors: Li L,Khan MN,Li Q,Chen X,Wei J,Wang B,Cheng JW,Gordon JR,Li F

    更新日期:2015-02-01 00:00:00

  • Ursolic acid inhibits growth and induces apoptosis in gemcitabine-resistant human pancreatic cancer via the JNK and PI3K/Akt/NF-κB pathways.

    abstract::Pancreatic cancer is one of the most deadly carcinomas worldwide. Although gemcitabine as the standard chemotherapy agent has been proven to be effective, the response rate remains at 5.4% and the 5-year survival rate is extremely poor. Ursolic acid (UA) is a small molecule compound...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2012.1827

    authors: Li J,Liang X,Yang X

    更新日期:2012-08-01 00:00:00

  • A phase II study of insulin-like growth factor receptor inhibition with nordihydroguaiaretic acid in men with non-metastatic hormone-sensitive prostate cancer.

    abstract::Insulin-like growth factor (IGF)-mediated signaling is a newly recognized clinical target in prostate cancer, and it is hypothesized that blockade of the IGF receptor (IGF1R) will impair downstream signaling and slow tumor growth. In this study the efficacy of nordihydroguaiaretic a...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2011.1487

    authors: Friedlander TW,Weinberg VK,Huang Y,Mi JT,Formaker CG,Small EJ,Harzstark AL,Lin AM,Fong L,Ryan CJ

    更新日期:2012-01-01 00:00:00

  • Sanguinarine-induced apoptosis in lung adenocarcinoma cells is dependent on reactive oxygen species production and endoplasmic reticulum stress.

    abstract::Sanguinarine (SAN), an alkaloid isolated from plants of the Papaveraceae family, is a compound with multiple biological activities. In the present study, we explored the anticancer properties of SAN in lung cancer using the human lung adenocarcinoma cell line SPC-A1. Our results revealed that SAN inhibited SPC-A1 cell...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4054

    authors: Gu S,Yang XC,Xiang XY,Wu Y,Zhang Y,Yan XY,Xue YN,Sun LK,Shao GG

    更新日期:2015-08-01 00:00:00

  • Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway.

    abstract::Dendropanax morbifera Leveille is found throughout southwestern Korea, and has been used in traditional medicine for various diseases, such as migraine headache, infectious diseases, skin diseases and dysmenorrhea. However, the molecular mechanisms of D. morbifera concerning its biochemical actions in cancer have not ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2013.2542

    authors: Lee JW,Park C,Han MH,Hong SH,Lee TK,Lee SH,Kim GY,Choi YH

    更新日期:2013-09-01 00:00:00

  • Expression of matrix metalloproteinases in human transitional cell carcinoma of the urinary bladder.

    abstract::Matrix metalloproteinases (MMPs) are associated with invasion and metastasis of several human malignant tumors. Especially so MMP-7, as it is mainly produced by the actual cancer cells. However, the expression of MMP-7 in transitional cell carcinoma (TCC) of the urinary bladder has not been previously investigated. We...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Sumi T,Yoshida H,Hyun Y,Yasui T,Matsumoto Y,Hattori K,Sugimura K,Kawashima H,Nakatani T,Ishiko O

    更新日期:2003-03-01 00:00:00

  • PBOV1 correlates with progression of ovarian cancer and inhibits proliferation of ovarian cancer cells.

    abstract::Prostate and breast cancer overexpressed 1 (PBOV1) is significantly upregulated in prostate, breast and bladder cancer, while its expression status in ovarian cancer and its clinical significance are unclear. We examined the expression levels of PBOV1 mRNA and protein in ovarian cancer cell lines and primary tissues u...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4396

    authors: Wang L,Niu CH,Wu S,Wu HM,Ouyang F,He M,He SY

    更新日期:2016-01-01 00:00:00

  • E26 transformation (ETS)‑specific related transcription factor‑3 (ELF3) orchestrates a positive feedback loop that constitutively activates the MAPK/Erk pathway to drive thyroid cancer.

    abstract::Thyroid cancer is the most commonly diagnosed malignancy of the endocrine system, the incidence of which has increased rapidly in the last 30 years. Genetic alterations in pathways, including the mitogen‑activated protein kinase (MAPK)/extracellular signal‑regulated kinase (Erk) and phosphatidylinositol‑3‑kinase (PI3K...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2018.6807

    authors: Chen H,Chen W,Zhang X,Hu L,Tang G,Kong J,Wang Z

    更新日期:2019-01-01 00:00:00

  • MicroRNA and target mRNA selection through invasion and cytotoxicity cell modeling and bioinformatics approaches in esophageal squamous cell carcinoma.

    abstract::This study analyzed microRNA (miRNA) and mRNA expression profiles and investigated the biological characteristics of ESCC by using invasion and cytotoxicity cell models. miRNA profiles were evaluated through miRNA microarray. Transwell chamber and nedaplatin (NDP) were used to construct invasion and cytotoxicity cell ...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2017.5776

    authors: Lu M,Song Y,Fu W,Liu Y,Huai S,Cui X,Pang L,Yang L,Wei Y

    更新日期:2017-08-01 00:00:00

  • Overall survival with and without laryngeal function preservation in 580 patients with hypopharyngeal squamous cell carcinoma.

    abstract::The aims of the present study were to review the experience of different surgical reconstruction methods for hypopharyngeal squamous cell carcinoma (HSCC) and compared the survival of patients with and without laryngeal function (LF) preservation. The clinical characteristics of 580 patients were retrospectively obtai...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.2015.4313

    authors: Zhou J,Li Y,Wei D,Qian Y,Li W,Liu D,Li G,Pan X,Lei D

    更新日期:2015-12-01 00:00:00

  • Plasma levels and expression of vascular endothelial growth factor-A in human localized prostate cancer.

    abstract::Although the impact of vascular endothelial growth factor (VEGF) is clearly established in advanced prostate cancer (PCa), its role in localized PCa remains to be determined. The aim of our study was to analyse the plasma levels of VEGF-A and the expression of VEGF-A in prostatic tissue in a population of patients wit...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:

    authors: Peyromaure M,Badoual C,Camparo P,Grabar S,Goulvestre C,Fulla Y,Vieillefond A,Mao K,Dinh-Xuan AT

    更新日期:2007-07-01 00:00:00

  • An antibody assay predictive of BRCA1 mutations in ovarian tumors and normal tissue.

    abstract::To determine whether immunohistochemistry can identify BRCA1 mutations, immunohistochemical (IH) analysis was undertaken on paraffin sections of paired ovarian cancer and normal tissue using antibodies against both terminal regions of the BRCA1 protein. Ten patients at risk for BRCA1 mutations were studied. The result...

    journal_title:Oncology reports

    pub_type: 杂志文章

    doi:10.3892/or.7.5.949

    authors: Byrne TJ,Reece MT,Adams LA,Lane MA,Cohn GM

    更新日期:2000-09-01 00:00:00