当前位置： SCI文献检索 > ACS Chemical Biology期刊下所有文献 > PDZ-Reactive Peptide Activates Ephrin-B Reverse Signaling and Inhibits Neuronal Chemotaxis.

PDZ-Reactive Peptide Activates Ephrin-B Reverse Signaling and Inhibits Neuronal Chemotaxis.

Abstract：

:Intracellular reactions on nonenzymatic proteins that activate cellular signals are rarely found. We report one example here that a designed peptide derivative undergoes a nucleophilic reaction specifically with a cytosolic PDZ protein inside cells. This reaction led to the activation of ephrin-B reverse signaling, which subsequently inhibited SDF-1 induced neuronal chemotaxis of human neuroblastoma cells and mouse cerebellar granule neurons. Our work provides direct evidence that PDZ-RGS3 bridges ephrin-B reverse signaling and SDF-1 induced G protein signaling for the first time.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Yu Y,Liu M,Ng TT,Huang F,Nie Y,Wang R,Yao ZP,Li Z,Xia J

doi

10.1021/acschembio.5b00889

subject

Has Abstract

pub_date

2016-01-15 00:00:00

pages

149-58

issue

1

eissn

1554-8929

issn

1554-8937

journal_volume

11

pub_type

杂志文章

相关文献

ACS Chemical Biology文献大全
  • Rational design, preparation, and characterization of a therapeutic enzyme mutant with improved stability and function for cocaine detoxification.

    abstract：:Cocaine esterase (CocE) is known as the most efficient natural enzyme for cocaine hydrolysis. The major obstacle to the clinical application of wild-type CocE is the thermoinstability with a half-life of only ∼12 min at 37 °C. The previously designed T172R/G173Q mutant (denoted as enzyme E172-173) with an improved in ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb500257s

    authors： Fang L,Chow KM,Hou S,Xue L,Chen X,Rodgers DW,Zheng F,Zhan CG

    更新日期：2014-08-15 00:00:00

  • Small Molecule Inhibition of miR-544 Biogenesis Disrupts Adaptive Responses to Hypoxia by Modulating ATM-mTOR Signaling.

    abstract：:Hypoxia induces a complex circuit of gene expression that drives tumor progression and increases drug resistance. Defining these changes allows for an understanding of how hypoxia alters tumor biology and informs design of lead therapeutics. We probed the role of microRNA-544 (miR-544), which silences mammalian target...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.5b00265

    authors： Haga CL,Velagapudi SP,Strivelli JR,Yang WY,Disney MD,Phinney DG

    更新日期：2015-10-16 00:00:00

  • O-Demethylations catalyzed by Rieske nonheme iron monooxygenases involve the difficult oxidation of a saturated C-H bond.

    abstract：:Dicamba monooxygenase (DMO) catalyzes the O-demethylation of dicamba (3,6-dichloro-2-methoxybenzoate) to produce 3,6-dichlorosalicylate and formaldehyde. Recent crystallographic studies suggest that DMO catalyzes the challenging oxidation of a saturated C-H bond within the methyl group of dicamba to form a hemiacetal ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400154a

    authors： Jiang W,Wilson MA,Weeks DP

    更新日期：2013-08-16 00:00:00

  • Structural Insights into the Interaction of Clinically Relevant Phosphorothioate mRNA Cap Analogs with Translation Initiation Factor 4E Reveal Stabilization via Electrostatic Thio-Effect.

    abstract：:mRNA-based therapies and vaccines constitute a disruptive technology with the potential to revolutionize modern medicine. Chemically modified 5' cap structures have provided access to mRNAs with superior translational properties that could benefit the currently flourishing mRNA field. Prime examples of compounds that ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00864

    authors： Warminski M,Kowalska J,Nowak E,Kubacka D,Tibble R,Kasprzyk R,Sikorski PJ,Gross JD,Nowotny M,Jemielity J

    更新日期：2021-01-13 00:00:00

  • Targeting Regorafenib-Induced Toxicity through Inhibition of Gut Microbial β-Glucuronidases.

    abstract：:Regorafenib (Stivarga) is an oral small molecule kinase inhibitor used to treat metastatic colorectal cancer, hepatocellular carcinomas, and gastrointestinal stromal tumors. Diarrhea is one of the most frequently observed adverse reactions associated with regorafenib. This toxicity may arise from the reactivation of t...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00663

    authors： Ervin SM,Hanley RP,Lim L,Walton WG,Pearce KH,Bhatt AP,James LI,Redinbo MR

    更新日期：2019-12-20 00:00:00

  • Characterization of Class IB Terpene Synthase: The First Crystal Structure Bound with a Substrate Surrogate.

    abstract：:Terpene synthases (TS) are classified into two broad types, Class I and II, based on the chemical strategy for initial carbocation formation and motif sequences of the catalytic site. We have recently identified a new class of enzymes, Class IB, showing the acceptability of long (C20-C35) prenyl-diphosphates as substr...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00145

    authors： Stepanova R,Inagi H,Sugawara K,Asada K,Nishi T,Ueda D,Yasuno Y,Shinada T,Miki K,Fujihashi M,Sato T

    更新日期：2020-06-19 00:00:00

  • Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers.

    abstract：:The T cell receptor (TCR)-cluster of differentiation 3 (CD3) signaling complex plays an important role in initiation of adaptive immune responses, but weak interactions have obstructed delineation of the individual TCR-CD3 subunit interactions during T cell signaling. Here, we demonstrate that unnatural amino acids (U...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb500351s

    authors： Wang W,Li T,Felsovalyi K,Chen C,Cardozo T,Krogsgaard M

    更新日期：2014-09-19 00:00:00

  • Evaluation of analogues of GalNAc as substrates for enzymes of the mammalian GalNAc salvage pathway.

    abstract：:Changes in glycosylation are correlated to disease and associated with differentiation processes. Experimental tools are needed to investigate the physiological implications of these changes either by labeling of the modified glycans or by blocking their biosynthesis. N-Acetylgalactosamine (GalNAc) is a monosaccharide...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb200511t

    authors： Pouilly S,Bourgeaux V,Piller F,Piller V

    更新日期：2012-04-20 00:00:00

  • Profiling Cysteine Reactivity and Oxidation in the Endoplasmic Reticulum.

    abstract：:The endoplasmic reticulum (ER) is the initial site of biogenesis of secretory pathway proteins, including proteins localized to the ER, Golgi, lysosomes, intracellular vesicles, plasma membrane, and extracellular compartments. Proteins within the secretory pathway contain a high abundance of disulfide bonds to protect...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b01014

    authors： Bechtel TJ,Li C,Kisty EA,Maurais AJ,Weerapana E

    更新日期：2020-02-21 00:00:00

  • Cell-penetrating bisubstrate-based protein kinase C inhibitors.

    abstract：:Although protein kinase inhibitors present excellent pharmaceutical opportunities, lack of selectivity and associated therapeutic side effects are common. Bisubstrate-based inhibitors targeting both the high-selectivity peptide substrate binding groove and the high-affinity ATP pocket address this. However, they are t...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300709g

    authors： van Wandelen LT,van Ameijde J,Ismail-Ali AF,van Ufford HC,Vijftigschild LA,Beekman JM,Martin NI,Ruijtenbeek R,Liskamp RM

    更新日期：2013-07-19 00:00:00

  • Peptides derived from the transmembrane domain of Bcl-2 proteins as potential mitochondrial priming tools.

    abstract：:The Bcl-2 family of proteins is crucial for apoptosis regulation. Members of this family insert through a specific C-terminal anchoring transmembrane domain (TMD) in the mitochondrial outer membrane where they hierarchically interact to determine cell fate. While the mitochondrial membrane has been proposed to activel...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb5002679

    authors： Andreu-Fernández V,Genoves A,Lee TH,Stellato M,Lucantoni F,Orzáez M,Mingarro I,Aguilar MI,Pérez-Payá E

    更新日期：2014-08-15 00:00:00

  • Effect of sequence and structural properties on 14-helical beta-peptide activity against Candida albicans planktonic cells and biofilms.

    abstract：:Beta-peptides (beta-amino acid oligomers) that mimic the amphiphilic, helical, and cationic properties of natural antimicrobial peptides have previously been shown to display antifungal activity against planktonic Candida albicans cells. Beta-peptides offer several advantages over conventional peptides composed of alp...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb900093r

    authors： Karlsson AJ,Pomerantz WC,Neilsen KJ,Gellman SH,Palecek SP

    更新日期：2009-07-17 00:00:00

  • Discovery of a nitric oxide responsive quorum sensing circuit in Vibrio harveyi.

    abstract：:Bacteria use small molecules to assess the density and identity of nearby organisms and formulate a response. This process, called quorum sensing (QS), commonly regulates bioluminescence, biofilm formation, and virulence. Vibrio harveyi have three described QS circuits. Each involves the synthesis of a molecule that r...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300215t

    authors： Henares BM,Higgins KE,Boon EM

    更新日期：2012-08-17 00:00:00

  • Retroviral display in gene therapy, protein engineering, and vaccine development.

    abstract：:The display and analysis of proteins expressed on biological surfaces has become an attractive tool for the study of molecular interactions in enzymology, protein engineering, and high-throughput screening. Among the growing number of established display systems, retroviruses offer a unique and fully mammalian platfor...

    journal_title：ACS chemical biology

    pub_type： 杂志文章,评审

    doi：10.1021/cb100285n

    authors： Urban JH,Merten CA

    更新日期：2011-01-21 00:00:00

  • Small molecule inhibitors of bromodomain-acetyl-lysine interactions.

    abstract：:Bromodomains are protein modules that bind to acetylated lysine residues. Their interaction with histone proteins suggests that they function as "readers" of histone lysine acetylation, a component of the proposed "histone code". Bromodomain-containing proteins are often found as components of larger protein complexes...

    journal_title：ACS chemical biology

    pub_type： 杂志文章,评审

    doi：10.1021/cb500996u

    authors： Brand M,Measures AR,Wilson BG,Cortopassi WA,Alexander R,Höss M,Hewings DS,Rooney TP,Paton RS,Conway SJ

    更新日期：2015-01-16 00:00:00

  • Bacterial Riboswitches and Ribozymes Potently Activate the Human Innate Immune Sensor PKR.

    abstract：:The innate immune system provides the first line of defense against pathogens through the recognition of nonspecific patterns in RNA to protect the cell in a generalized way. The human RNA-activated protein kinase, PKR, is a dsRNA binding protein and an essential sensor in the innate immune response, which recognizes ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.6b00081

    authors： Hull CM,Anmangandla A,Bevilacqua PC

    更新日期：2016-04-15 00:00:00

  • An Isotope-Coded Photocleavable Probe for Quantitative Profiling of Protein O-GlcNAcylation.

    abstract：:O-linked N-acetylglucosamine ( O-GlcNAc) is a ubiquitous post-translational modification of proteins and is essential for cell function. Quantifying the dynamics of O-GlcNAcylation in a proteome-wide level is critical for uncovering cellular mechanisms and functional roles of O-GlcNAcylation in cells. Here, we develop...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.8b01052

    authors： Li J,Li Z,Duan X,Qin K,Dang L,Sun S,Cai L,Hsieh-Wilson LC,Wu L,Yi W

    更新日期：2019-01-18 00:00:00

  • Antiviral activity of an isatin derivative via induction of PERK-Nrf2-mediated suppression of cap-independent translation.

    abstract：:We report here an isatin derivative 45 (ID45) against coxsackievirus B3 (CVB3) replication, which was synthesized based on a high-throughput screen of a unique natural product library. ID45 showed the most potent anti-CVB3 activity among the four synthesized compounds. Treatment of cells with ID45 before or after infe...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400775z

    authors： Zhang HM,Dai H,Hanson PJ,Li H,Guo H,Ye X,Hemida MG,Wang L,Tong Y,Qiu Y,Liu S,Wang F,Song F,Zhang B,Wang JG,Zhang LX,Yang D

    更新日期：2014-04-18 00:00:00

  • Catalytic mechanisms and biocatalytic applications of aspartate and methylaspartate ammonia lyases.

    abstract：:Ammonia lyases catalyze the formation of α,β-unsaturated bonds by the elimination of ammonia from their substrates. This conceptually straightforward reaction has been the emphasis of many studies, with the main focus on the catalytic mechanism of these enzymes and/or the use of these enzymes as catalysts for the synt...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb3002792

    authors： de Villiers M,Puthan Veetil V,Raj H,de Villiers J,Poelarends GJ

    更新日期：2012-10-19 00:00:00

  • In vitro selection of anti-Akt2 thioether-macrocyclic peptides leading to isoform-selective inhibitors.

    abstract：:The Akt kinase family, consisting of three isoforms in humans, is a well-validated class of drug target. Through various screening campaigns in academics and pharmaceutical industries, several promising inhibitors have been developed to date. However, due to the mechanistic and structural similarities of Akt kinases, ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb200388k

    authors： Hayashi Y,Morimoto J,Suga H

    更新日期：2012-03-16 00:00:00

  • Cytochrome c Reduction by H2S Potentiates Sulfide Signaling.

    abstract：:Hydrogen sulfide (H2S) is an endogenously produced gas that is toxic at high concentrations. It is eliminated by a dedicated mitochondrial sulfide oxidation pathway, which connects to the electron transfer chain at the level of complex III. Direct reduction of cytochrome c (Cyt C) by H2S has been reported previously b...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.8b00463

    authors： Vitvitsky V,Miljkovic JL,Bostelaar T,Adhikari B,Yadav PK,Steiger AK,Torregrossa R,Pluth MD,Whiteman M,Banerjee R,Filipovic MR

    更新日期：2018-08-17 00:00:00

  • Analysis of amyloid nanostructures using photo-cross-linking: in situ comparison of three widely used photo-cross-linkers.

    abstract：:Photoinduced cross-linking (PIC) has become a powerful tool in chemical biology for the identification and mapping of stable or transient interactions between biomacromolecules and their (unknown) ligands. However, the value of PIC for in vitro and in vivo structural proteomics can be realized only if cross-linking re...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400731s

    authors： Preston GW,Radford SE,Ashcroft AE,Wilson AJ

    更新日期：2014-03-21 00:00:00

  • Linking High-Throughput Screens to Identify MoAs and Novel Inhibitors of Mycobacterium tuberculosis Dihydrofolate Reductase.

    abstract：:Though phenotypic and target-based high-throughput screening approaches have been employed to discover new antibiotics, the identification of promising therapeutic candidates remains challenging. Each approach provides different information, and understanding their results can provide hypotheses for a mechanism of act...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.7b00468

    authors： Santa Maria JP Jr,Park Y,Yang L,Murgolo N,Altman MD,Zuck P,Adam G,Chamberlin C,Saradjian P,Dandliker P,Boshoff HIM,Barry CE 3rd,Garlisi C,Olsen DB,Young K,Glick M,Nickbarg E,Kutchukian PS

    更新日期：2017-09-15 00:00:00

  • Mutations of Triad Determinants Changes the Substrate Alignment at the Catalytic Center of Human ALOX5.

    abstract：:For the specificity of ALOX15 orthologs of different mammals, the geometry of the amino acids Phe353, Ile418, Met419, and Ile593 ("triad determinants") is important, and mutagenesis of these residues altered the reaction specificity of these enzymes. Here we expressed wild-type human ALOX5 and its F359W/A424I/N425M/A6...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00674

    authors： Ivanov I,Golovanov AB,Ferretti C,Canyelles-Niño M,Heydeck D,Stehling S,Lluch JM,González-Lafont À,Kühn H

    更新日期：2019-12-20 00:00:00

  • Adversarial Controls for Scientific Machine Learning.

    abstract：:New machine learning methods to analyze raw chemical and biological data are now widely accessible as open-source toolkits. This positions researchers to leverage powerful, predictive models in their own domains. We caution, however, that the application of machine learning to experimental research merits careful cons...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.8b00881

    authors： Chuang KV,Keiser MJ

    更新日期：2018-10-19 00:00:00

  • 3-Oxo-β-sultam as a Sulfonylating Chemotype for Inhibition of Serine Hydrolases and Activity-Based Protein Profiling.

    abstract：:3-Oxo-β-sultams are four-membered ring ambident electrophiles that can react with nucleophiles either at the carbonyl carbon or at the sulfonyl sulfur atoms, and that have been reported to inhibit serine hydrolases via acylation of the active-site serine residue. We have developed a panel of 3-oxo-β-sultam inhibitors ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00090

    authors： Carvalho LAR,Almeida VT,Brito JA,Lum KM,Oliveira TF,Guedes RC,Gonçalves LM,Lucas SD,Cravatt BF,Archer M,Moreira R

    更新日期：2020-04-17 00:00:00

  • Defined substrates for human embryonic stem cell growth identified from surface arrays.

    abstract：:Methods for the rapid identification of defined cell growth conditions are lacking. This deficiency is a major barrier to the investigation and application of human embryonic stem (ES) cells. To address this problem, we developed a method for generating arrays of self-assembled monolayers (SAMs) in which each element ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb700032u

    authors： Derda R,Li L,Orner BP,Lewis RL,Thomson JA,Kiessling LL

    更新日期：2007-05-22 00:00:00

  • Biochemical and biophysical analysis of a chiral PqsD inhibitor revealing tight-binding behavior and enantiomers with contrary thermodynamic signatures.

    abstract：:Antivirulence strategies addressing bacterial pathogenicity without exhibiting growth inhibition effects represent a novel approach to overcome today's crisis in antibiotic development. In recent studies, we examined various inhibitors of PqsD, an enzyme involved in formation of Pseudomonas aeruginosa cell-to-cell sig...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400530d

    authors： Storz MP,Brengel C,Weidel E,Hoffmann M,Hollemeyer K,Steinbach A,Müller R,Empting M,Hartmann RW

    更新日期：2013-12-20 00:00:00

  • Studying weak and dynamic interactions of posttranslationally modified proteins using expressed protein ligation.

    abstract：:Many cellular processes are regulated by posttranslational modifications that are recognized by specific domains in protein binding partners. These interactions are often weak, thus allowing a highly dynamic and combinatorial regulatory network of protein-protein interactions. We report an efficient strategy that over...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400723j

    authors： Tripsianes K,Chu NK,Friberg A,Sattler M,Becker CF

    更新日期：2014-02-21 00:00:00

  • Crystallographic fragment screening and structure-based optimization yields a new class of influenza endonuclease inhibitors.

    abstract：:Seasonal and pandemic influenza viruses continue to be a leading global health concern. Emerging resistance to the current drugs and the variable efficacy of vaccines underscore the need for developing new flu drugs that will be broadly effective against wild-type and drug-resistant influenza strains. Here, we report ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400400j

    authors： Bauman JD,Patel D,Baker SF,Vijayan RS,Xiang A,Parhi AK,Martínez-Sobrido L,LaVoie EJ,Das K,Arnold E

    更新日期：2013-11-15 00:00:00