当前位置： SCI文献检索 > ACS Chemical Biology期刊下所有文献 > Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers.

Quantitative analysis of T cell receptor complex interaction sites using genetically encoded photo-cross-linkers.

Abstract：

:The T cell receptor (TCR)-cluster of differentiation 3 (CD3) signaling complex plays an important role in initiation of adaptive immune responses, but weak interactions have obstructed delineation of the individual TCR-CD3 subunit interactions during T cell signaling. Here, we demonstrate that unnatural amino acids (UAA) can be used to photo-cross-link subunits of TCR-CD3 on the cell surface. Incorporating UAA in mammalian cells is usually a low efficiency process. In addition, TCR-CD3 is composed of eight subunits and both TCR and CD3 chains are required for expression on the cell surface. Photo-cross-linking of UAAs for studying protein complexes such as TCR-CD3 is challenging due to the difficulty of transfecting and expressing multisubunit protein complexes in cells combined with the low efficiency of UAA incorporation. Here, we demonstrate that by systematic optimization, we can incorporate UAA in TCR-CD3 with high efficiency. Accordingly, the incorporated UAA can be used for site-specific photo-cross-linking experiments to pinpoint protein interaction sites, as well as to confirm interaction sites identified by X-ray crystallography. We systemically compared two different photo-cross-linkers--p-azido-phenylalanine (pAzpa) and H-p-Bz-Phe-OH (pBpa)--for their ability to map protein subunit interactions in the 2B4 TCR. pAzpa was found to have higher cross-linking efficiency, indicating that optimization of the selection of the most optimal cross-linker is important for correct identification of protein-protein interactions. This method is therefore suitable for studying interaction sites of large, dynamic heteromeric protein complexes associated with various cellular membrane systems.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Wang W,Li T,Felsovalyi K,Chen C,Cardozo T,Krogsgaard M

doi

10.1021/cb500351s

subject

Has Abstract

pub_date

2014-09-19 00:00:00

pages

2165-72

issue

9

eissn

1554-8929

issn

1554-8937

journal_volume

9

pub_type

杂志文章

相关文献

ACS Chemical Biology文献大全
  • Biochemical and Structural Investigation of GnnA in the Lipopolysaccharide Biosynthesis Pathway of Acidithiobacillus ferrooxidans.

    abstract：:Lipopolysaccharide (LPS) is a crucial component in the outer membrane of Gram-negative bacteria that contributes to both pathogenicity as well as immunity against pathogenic bacteria. Typical LPS contains GlcN disaccharide as the core of lipid A. However, some bacteria such as Acidithiobacillus ferrooxidans and Leptos...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00791

    authors： Manissorn J,Sitthiyotha T,Montalban JRE,Chunsrivirot S,Thongnuek P,Wangkanont K

    更新日期：2020-12-18 00:00:00

  • 2D NMR-based metabolomics uncovers interactions between conserved biochemical pathways in the model organism Caenorhabditis elegans.

    abstract：:Ascarosides are small-molecule signals that play a central role in C. elegans biology, including dauer formation, aging, and social behaviors, but many aspects of their biosynthesis remain unknown. Using automated 2D NMR-based comparative metabolomics, we identified ascaroside ethanolamides as shunt metabolites in C. ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb3004644

    authors： Izrayelit Y,Robinette SL,Bose N,von Reuss SH,Schroeder FC

    更新日期：2013-02-15 00:00:00

  • Phenotypic Screen Identifies JAK2 as a Major Regulator of FAT10 Expression.

    abstract：:FAT10 is a ubiquitin-like protein suggested to target proteins for proteasomal degradation. It is highly upregulated upon pro-inflammatory cytokines, namely, TNFα, IFNγ, and IL6, and was found to be highly expressed in various epithelial cancers. Evidence suggests that FAT10 is involved in cancer development and may h...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00667

    authors： Reznik N,Kozer N,Eisenberg-Lerner A,Barr H,Merbl Y,London N

    更新日期：2019-12-20 00:00:00

  • Evaluation of analogues of GalNAc as substrates for enzymes of the mammalian GalNAc salvage pathway.

    abstract：:Changes in glycosylation are correlated to disease and associated with differentiation processes. Experimental tools are needed to investigate the physiological implications of these changes either by labeling of the modified glycans or by blocking their biosynthesis. N-Acetylgalactosamine (GalNAc) is a monosaccharide...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb200511t

    authors： Pouilly S,Bourgeaux V,Piller F,Piller V

    更新日期：2012-04-20 00:00:00

  • Parietal Structures of Escherichia coli Can Impact the D-Cateslytin Antibacterial Activity.

    abstract：:Bacterial resistance to conventional antibiotics is of major concern. Antimicrobial peptides (AMPs) are considered excellent alternatives. Among them, D-cateslytin (D-Ctl, derivative of a host defense peptide) has shown high efficiency against a broad spectrum of bacteria. The first target of AMPs is the outer membran...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00622

    authors： Quilès F,Barth D,Peric O,Fantner GE,Francius G

    更新日期：2020-10-16 00:00:00

  • Heterologous Biosynthesis of Type II Polyketide Products Using E. coli.

    abstract：:The heterologous biosynthesis of complex natural products has enabled access to polyketide, nonribosomal peptide, isoprenoid, and other compounds with wide-spanning societal value. Though several surrogate host systems exist, Escherichia coli is often a preferred choice due to its rapid growth kinetics and extensive m...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00827

    authors： Liu X,Hua K,Liu D,Wu ZL,Wang Y,Zhang H,Deng Z,Pfeifer BA,Jiang M

    更新日期：2020-05-15 00:00:00

  • The STAT5b Linker Domain Mediates the Selectivity of Catechol Bisphosphates for STAT5b over STAT5a.

    abstract：:STAT family proteins are important mediators of cell signaling and represent therapeutic targets for the treatment of human diseases. Most STAT inhibitors target the protein-protein interaction domain, the SH2 domain, but specificity for a single STAT protein is often limited. Recently, we developed catechol bisphosph...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00137

    authors： Gräb J,Berg A,Blechschmidt L,Klüver B,Rubner S,Fu DY,Meiler J,Gräber M,Berg T

    更新日期：2019-04-19 00:00:00

  • The N-Terminal Domain of NPHP1 Folds into a Monomeric Left-Handed Antiparallel Three-Stranded Coiled Coil with Anti-apoptotic Function.

    abstract：:Mutations in the NPHP1 gene, coding for human nephrocystin-1 (NPHP1), cause the autosomal recessive disease nephronophthisis, the most common cause of end-stage renal disease in children and adolescents. The function and structure of NPHP1 are still poorly characterized. NPHP1 presents a modular structure well in keep...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00582

    authors： Mannella V,Quilici G,Nigro EA,Lampis M,Minici C,Degano M,Boletta A,Musco G

    更新日期：2019-08-16 00:00:00

  • Chemical probes of surface layer biogenesis in Clostridium difficile.

    abstract：:Clostridium difficile, a leading cause of hospital-acquired infection, possesses a dense surface layer (S-layer) that mediates host-pathogen interactions. The key structural components of the S-layer result from proteolytic cleavage of a precursor protein, SlpA, into high- and low-molecular-weight components. Here we ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb9002859

    authors： Dang TH,de la Riva L,Fagan RP,Storck EM,Heal WP,Janoir C,Fairweather NF,Tate EW

    更新日期：2010-03-19 00:00:00

  • A molecular-beacon-based screen for small molecule inhibitors of miRNA maturation.

    abstract：:miRNAs are small non-coding RNAs that regulate about 60% of mammalian genes by modulating their transcript levels. Network scale studies of miRNA-mediated regulatory circuits demonstrate the central importance of this class of small RNA in the maintenance of biological robustness. More recently, several reports have d...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300650y

    authors： Bose D,Jayaraj GG,Kumar S,Maiti S

    更新日期：2013-05-17 00:00:00

  • Cruentaren A binds F1F0 ATP synthase to modulate the Hsp90 protein folding machinery.

    abstract：:The molecular chaperone Hsp90 requires the assistance of immunophilins, co-chaperones, and partner proteins for the conformational maturation of client proteins. Hsp90 inhibition represents a promising anticancer strategy due to the dependence of numerous oncogenic signaling pathways upon Hsp90 function. Historically,...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400906e

    authors： Hall JA,Kusuma BR,Brandt GE,Blagg BS

    更新日期：2014-04-18 00:00:00

  • pH-Dependent DNA Distortion and Repression of Gene Expression by Pectobacterium atrosepticum PecS.

    abstract：:Transcriptional activity is exquisitely sensitive to changes in promoter DNA topology. Transcription factors may therefore control gene activity by modulating the relative positioning of -10 and -35 promoter elements. The plant pathogen Pectobacterium atrosepticum, which causes soft rot in potatoes, must alter gene ex...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.6b00168

    authors： Deochand DK,Meariman JK,Grove A

    更新日期：2016-07-15 00:00:00

  • Structural and Biosynthetic Analysis of the Fabrubactins, Unusual Siderophores from Agrobacterium fabrum Strain C58.

    abstract：:Siderophores are iron-chelating molecules produced by microorganisms and plants to acquire exogenous iron. Siderophore biosynthetic enzymology often produces elaborate and unique molecules through unusual reactions to enable specific recognition by the producing organisms. Herein, we report the structure of two sidero...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00809

    authors： Vinnik V,Zhang F,Park H,Cook TB,Throckmorton K,Pfleger BF,Bugni TS,Thomas MG

    更新日期：2021-01-15 00:00:00

  • Aggregation-mediated macromolecular uptake by a molecular transporter.

    abstract：:Endocytosis is a key process in cellular delivery of macromolecules by molecular transporters, although the mechanism of internalization remains unclear. Here, we probe the cellular uptake of streptavidin using biotinylated guanidinoneomycin (biotinGNeo), a low molecular weight guanidinium-rich molecular transporter. ...

    journal_title：ACS chemical biology

    pub_type： 信件

    doi：10.1021/cb400172h

    authors： Inoue M,Tong W,Esko JD,Tor Y

    更新日期：2013-07-19 00:00:00

  • Calcium-dependent ligand binding and G-protein signaling of family B GPCR parathyroid hormone 1 receptor purified in nanodiscs.

    abstract：:GPCRs mediate intracellular signaling upon external stimuli, making them ideal drug targets. However, little is known about their activation mechanisms due to the difficulty in purification. Here, we introduce a method to purify GPCRs in nanodiscs, which incorporates GPCRs into lipid bilayers immediately after membran...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300466n

    authors： Mitra N,Liu Y,Liu J,Serebryany E,Mooney V,DeVree BT,Sunahara RK,Yan EC

    更新日期：2013-03-15 00:00:00

  • Systematic Synthesis and Binding Study of HIV V3 Glycopeptides Reveal the Fine Epitopes of Several Broadly Neutralizing Antibodies.

    abstract：:A class of new glycan-reactive broadly neutralizing antibodies represented by PGT121, 10-1074, and PGT128 has recently been discovered that targets specific N-glycans and the peptide region around the V3 domain. However, the glycan specificity and fine epitopes of these bNAbs remain to be further defined. We report he...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.7b00319

    authors： Orwenyo J,Cai H,Giddens J,Amin MN,Toonstra C,Wang LX

    更新日期：2017-06-16 00:00:00

  • Structural Basis of Substrate Specificity in Geobacter metallireducens SMUG1.

    abstract：:Base deamination is a common type of DNA damage that occurs in all organisms. DNA repair mechanisms are critical to maintain genome integrity, in which the base excision repair pathway plays an essential role. In the BER pathway, the uracil DNA glycosylase superfamily is responsible for removing the deaminated bases f...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.6b00164

    authors： Zhang Z,Shen J,Yang Y,Li J,Cao W,Xie W

    更新日期：2016-06-17 00:00:00

  • Discovery of Novel Antibiotics as Covalent Inhibitors of Fatty Acid Synthesis.

    abstract：:The steady increase in the prevalence of multidrug-resistant Staphylococcus aureus has made the search for novel antibiotics to combat this clinically important pathogen an urgent matter. In an effort to discover antibacterials with new chemical structures and mechanisms, we performed a growth inhibition screen of a s...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00982

    authors： Wang J,Ye X,Yang X,Cai Y,Wang S,Tang J,Sachdeva M,Qian Y,Hu W,Leeds JA,Yuan Y

    更新日期：2020-07-17 00:00:00

  • Functional evaluation of key interactions evident in the structure of the eukaryotic Cys-loop receptor GluCl.

    abstract：:The publication of the first high-resolution crystal structure of a eukaryotic Cys-loop receptor, GluClα, has provided valuable structural information on this important class of ligand-gated ion channels (LGIC). However, limited functional data exist for the GluCl receptors. Before applying the structural insights fro...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb500323d

    authors： Daeffler KN,Lester HA,Dougherty DA

    更新日期：2014-10-17 00:00:00

  • Structural Studies Revealed Active Site Distortions of Human Furin by a Small Molecule Inhibitor.

    abstract：:Proprotein convertases (PCs) represent highly selective serine proteases that activate their substrates upon proteolytic cleavage. Their inhibition is a promising strategy for the treatment of several pathologies including cancer, atherosclerosis, hypercholesterolaemia, and infectious diseases. Here, we present the fi...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.6b01110

    authors： Dahms SO,Jiao GS,Than ME

    更新日期：2017-05-19 00:00:00

  • Chemical detoxification of small molecules by Caenorhabditis elegans.

    abstract：:Caenorhabditis elegans lives in compost and decaying fruit, eats bacteria and is exposed to pathogenic microbes. We show that C. elegans is able to modify diverse microbial small-molecule toxins via both O- and N-glucosylation as well as unusual 3'-O-phosphorylation of the resulting glucosides. The resulting glucosyla...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300520u

    authors： Stupp GS,von Reuss SH,Izrayelit Y,Ajredini R,Schroeder FC,Edison AS

    更新日期：2013-02-15 00:00:00

  • A chemical biological strategy to facilitate diabetic wound healing.

    abstract：:A complication of diabetes is the inability of wounds to heal in diabetic patients. Diabetic wounds are refractory to healing due to the involvement of activated matrix metalloproteinases (MMPs), which remodel the tissue resulting in apoptosis. There are no readily available methods that identify active unregulated MM...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb4005468

    authors： Gooyit M,Peng Z,Wolter WR,Pi H,Ding D,Hesek D,Lee M,Boggess B,Champion MM,Suckow MA,Mobashery S,Chang M

    更新日期：2014-01-17 00:00:00

  • On-chip synthesis and screening of a sialoside library yields a high affinity ligand for Siglec-7.

    abstract：:The Siglec family of sialic acid-binding proteins are differentially expressed on white blood cells of the immune system and represent an attractive class of targets for cell-directed therapy. Nanoparticles decorated with high-affinity Siglec ligands show promise for delivering cargo to Siglec-bearing cells, but this ...

    journal_title：ACS chemical biology

    pub_type： 信件

    doi：10.1021/cb400125w

    authors： Rillahan CD,Schwartz E,Rademacher C,McBride R,Rangarajan J,Fokin VV,Paulson JC

    更新日期：2013-07-19 00:00:00

  • Development of a selective activity-based probe for adenylating enzymes: profiling MbtA Involved in siderophore biosynthesis from Mycobacterium tuberculosis.

    abstract：:MbtA is an adenylating enzyme from Mycobacterium tuberculosis that catalyzes the first step in the biosynthesis of the mycobactins. A bisubstrate inhibitor of MbtA (Sal-AMS) was previously described that displays potent antitubercular activity under iron-replete as well as iron-deficient growth conditions. This findin...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300112x

    authors： Duckworth BP,Wilson DJ,Nelson KM,Boshoff HI,Barry CE 3rd,Aldrich CC

    更新日期：2012-10-19 00:00:00

  • Cytosolic Delivery of Macromolecules in Live Human Cells Using the Combined Endosomal Escape Activities of a Small Molecule and Cell Penetrating Peptides.

    abstract：:Ineffective cellular delivery is a common problem in numerous biological applications. Developing delivery reagents that work robustly in a variety of experimental settings remains a challenge. Herein, we report how peptides derived from the prototypical cell penetrating peptide TAT can be used in combination with a s...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00585

    authors： Allen J,Najjar K,Erazo-Oliveras A,Kondow-McConaghy HM,Brock DJ,Graham K,Hager EC,Marschall ALJ,Dübel S,Juliano RL,Pellois JP

    更新日期：2019-12-20 00:00:00

  • NMR structure of the S-linked glycopeptide sublancin 168.

    abstract：:Sublancin 168 is a member of a small group of glycosylated antimicrobial peptides known as glycocins. The solution structure of sublancin 168, a 37-amino-acid peptide produced by Bacillus subtilis 168, has been solved by nuclear magnetic resonance (NMR) spectroscopy. Sublancin comprises two α-helices and a well-define...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb4008106

    authors： Garcia De Gonzalo CV,Zhu L,Oman TJ,van der Donk WA

    更新日期：2014-03-21 00:00:00

  • Chemical Modulation of Human Mitochondrial ClpP: Potential Application in Cancer Therapeutics.

    abstract：:The human ClpP proteolytic complex (HsClpP) is a serine protease located in the mitochondrial matrix and participates in the maintenance of the mitochondrial proteome among other cellular functions. HsClpP typically forms a multimeric complex with the AAA+ protein unfoldase HsClpX. Notably, compared to that of normal,...

    journal_title：ACS chemical biology

    pub_type： 杂志文章,评审

    doi：10.1021/acschembio.9b00347

    authors： Wong KS,Houry WA

    更新日期：2019-11-15 00:00:00

  • Peptide-based investigation of the Escherichia coli RNA polymerase σ(70):core interface as target site.

    abstract：:The number of bacterial strains that are resistant against antibiotics increased dramatically during the past decades. This fact stresses the urgent need for the development of new antibacterial agents with novel modes of action targeting essential enzymes such as RNA polymerase (RNAP). Bacterial RNAP is a large multi...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb3005758

    authors： Hüsecken K,Negri M,Fruth M,Boettcher S,Hartmann RW,Haupenthal J

    更新日期：2013-04-19 00:00:00

  • Inhibition of WTA synthesis blocks the cooperative action of PBPs and sensitizes MRSA to β-lactams.

    abstract：:Rising drug resistance is limiting treatment options for infections by methicillin-resistant Staphylococcus aureus (MRSA). Herein we provide new evidence that wall teichoic acid (WTA) biogenesis is a remarkable antibacterial target with the capacity to destabilize the cooperative action of penicillin-binding proteins ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300413m

    authors： Farha MA,Leung A,Sewell EW,D'Elia MA,Allison SE,Ejim L,Pereira PM,Pinho MG,Wright GD,Brown ED

    更新日期：2013-01-18 00:00:00

  • Chemoproteomics-Enabled Covalent Ligand Screening Reveals ALDH3A1 as a Lung Cancer Therapy Target.

    abstract：:Chemical genetics is a powerful approach for identifying therapeutically active small molecules, but identifying the mechanisms of action underlying hit compounds remains challenging. Chemoproteomic platforms have arisen to tackle this challenge and enable rapid mechanistic deconvolution of small-molecule screening hi...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.8b00381

    authors： Counihan JL,Wiggenhorn AL,Anderson KE,Nomura DK

    更新日期：2018-08-17 00:00:00