当前位置： SCI文献检索 > ACS Chemical Biology期刊下所有文献 > An Isotope-Coded Photocleavable Probe for Quantitative Profiling of Protein O-GlcNAcylation.

An Isotope-Coded Photocleavable Probe for Quantitative Profiling of Protein O-GlcNAcylation.

Abstract：

:O-linked N-acetylglucosamine ( O-GlcNAc) is a ubiquitous post-translational modification of proteins and is essential for cell function. Quantifying the dynamics of O-GlcNAcylation in a proteome-wide level is critical for uncovering cellular mechanisms and functional roles of O-GlcNAcylation in cells. Here, we develop an isotope-coded photocleavable probe for profiling protein O-GlcNAcylation dynamics using quantitative mass spectrometry-based proteomics. This probe enables selective tagging and isotopic labeling of O-GlcNAcylated proteins in one step from complex cellular mixtures. We demonstrate the application of the probe to quantitatively profile O-GlcNAcylation sites in 293T cells upon chemical induction of O-GlcNAc levels. We further applied the probe to quantitatively analyze the stoichiometry of O-GlcNAcylation between sorafenib-sensitive and sorafenib-resistant liver cancer cells, which lays the foundation for mechanistic investigation of O-GlcNAcylation in regulating cancer chemoresistance. Thus, this probe provides a powerful tool to profile O-GlcNAcylation dynamics in cells.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Li J,Li Z,Duan X,Qin K,Dang L,Sun S,Cai L,Hsieh-Wilson LC,Wu L,Yi W

doi

10.1021/acschembio.8b01052

subject

Has Abstract

pub_date

2019-01-18 00:00:00

pages

4-10

issue

1

eissn

1554-8929

issn

1554-8937

journal_volume

14

pub_type

杂志文章

相关文献

ACS Chemical Biology文献大全
  • Development of a selective activity-based probe for adenylating enzymes: profiling MbtA Involved in siderophore biosynthesis from Mycobacterium tuberculosis.

    abstract：:MbtA is an adenylating enzyme from Mycobacterium tuberculosis that catalyzes the first step in the biosynthesis of the mycobactins. A bisubstrate inhibitor of MbtA (Sal-AMS) was previously described that displays potent antitubercular activity under iron-replete as well as iron-deficient growth conditions. This findin...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300112x

    authors： Duckworth BP,Wilson DJ,Nelson KM,Boshoff HI,Barry CE 3rd,Aldrich CC

    更新日期：2012-10-19 00:00:00

  • Extreme entropy-enthalpy compensation in a drug-resistant variant of HIV-1 protease.

    abstract：:The development of HIV-1 protease inhibitors has been the historic paradigm of rational structure-based drug design, where structural and thermodynamic analyses have assisted in the discovery of novel inhibitors. While the total enthalpy and entropy change upon binding determine the affinity, often the thermodynamics ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300191k

    authors： King NM,Prabu-Jeyabalan M,Bandaranayake RM,Nalam MN,Nalivaika EA,Özen A,Haliloğlu T,Yilmaz NK,Schiffer CA

    更新日期：2012-09-21 00:00:00

  • Structural basis of the promiscuous inhibitor susceptibility of Escherichia coli LpxC.

    abstract：:The LpxC enzyme in the lipid A biosynthetic pathway is one of the most promising and clinically unexploited antibiotic targets for treatment of multidrug-resistant Gram-negative infections. Progress in medicinal chemistry has led to the discovery of potent LpxC inhibitors with a variety of chemical scaffolds and disti...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb400067g

    authors： Lee CJ,Liang X,Gopalaswamy R,Najeeb J,Ark ED,Toone EJ,Zhou P

    更新日期：2014-01-17 00:00:00

  • Douglas Weibel: using microfluidics for microbiology.

    abstract：:The ubiquity of microorganisms is unparalleled in any other known organism. These creatures surround our outsides and colonize our insides, a fact that has been known for centuries. However, despite their prevalence and long study, many of their characteristics still remain largely unexplained, including how proteins ...

    journal_title：ACS chemical biology

    pub_type： 传,历史文章,杂志文章

    doi：10.1021/cb100179t

    authors： Brownlee C

    更新日期：2010-07-16 00:00:00

  • Modular strategy for the semisynthesis of a K+ channel: investigating interactions of the pore helix.

    abstract：:Chemical synthesis is a powerful method for precise modification of the structural and electronic properties of proteins. The difficulties in the synthesis and purification of peptides containing transmembrane segments have presented obstacles to the chemical synthesis of integral membrane proteins. Here, we present a...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb900210r

    authors： Komarov AG,Linn KM,Devereaux JJ,Valiyaveetil FI

    更新日期：2009-12-18 00:00:00

  • Probing enzymes late in the trypanosomal glycosylphosphatidylinositol biosynthetic pathway with synthetic glycosylphosphatidylinositol analogues.

    abstract：:Glycosylphosphatidylinositol (GPI)-anchored proteins are abundant in the protozoan parasite Trypanosoma brucei, the causative agent of African sleeping sickness in humans and the related disease Nagana in cattle, and disruption of GPI biosynthesis is genetically and chemically validated as a drug target. Here, we exam...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb800143w

    authors： Urbaniak MD,Yashunsky DV,Crossman A,Nikolaev AV,Ferguson MA

    更新日期：2008-10-17 00:00:00

  • Synthetic lethal compound combinations reveal a fundamental connection between wall teichoic acid and peptidoglycan biosyntheses in Staphylococcus aureus.

    abstract：:Methicillin resistance in Staphylococcus aureus depends on the production of mecA, which encodes penicillin-binding protein 2A (PBP2A), an acquired peptidoglycan transpeptidase (TP) with reduced susceptibility to β-lactam antibiotics. PBP2A cross-links nascent peptidoglycan when the native TPs are inhibited by β-lacta...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb100269f

    authors： Campbell J,Singh AK,Santa Maria JP Jr,Kim Y,Brown S,Swoboda JG,Mylonakis E,Wilkinson BJ,Walker S

    更新日期：2011-01-21 00:00:00

  • Characterization of CYP115 As a Gibberellin 3-Oxidase Indicates That Certain Rhizobia Can Produce Bioactive Gibberellin A4.

    abstract：:The gibberellin (GA) phytohormones are produced not only by plants but also by fungi and bacteria. Previous characterization of a cytochrome P450 (CYP)-rich GA biosynthetic operon found in many symbiotic, nitrogen-fixing rhizobia led to the elucidation of bacterial GA biosynthesis and implicated GA9 as the final produ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.6b01038

    authors： Nett RS,Contreras T,Peters RJ

    更新日期：2017-04-21 00:00:00

  • Proposed Mode of Binding and Action of Positive Allosteric Modulators at Opioid Receptors.

    abstract：:Available crystal structures of opioid receptors provide a high-resolution picture of ligand binding at the primary ("orthosteric") site, that is, the site targeted by endogenous ligands. Recently, positive allosteric modulators of opioid receptors have also been discovered, but their modes of binding and action remai...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.5b00712

    authors： Shang Y,Yeatman HR,Provasi D,Alt A,Christopoulos A,Canals M,Filizola M

    更新日期：2016-05-20 00:00:00

  • Antagonists for Constitutively Active Mutant Estrogen Receptors: Insights into the Roles of Antiestrogen-Core and Side-Chain.

    abstract：:A major risk for patients having estrogen receptor α (ERα)-positive breast cancer is the recurrence of drug-resistant metastases after initial successful treatment with endocrine therapies. Recent studies have implicated a number of activating mutations in the ligand-binding domain of ERα that stabilize the agonist co...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.8b00877

    authors： Sharma A,Toy W,Guillen VS,Sharma N,Min J,Carlson KE,Mayne CG,Lin S,Sabio M,Greene G,Katzenellenbogen BS,Chandarlapaty S,Katzenellenbogen JA

    更新日期：2018-12-21 00:00:00

  • Fragment Assembly Approach Based on Graph/Network Theory with Quantum Chemistry Verifications for Assigning Multidimensional NMR Signals in Metabolite Mixtures.

    abstract：:The abundant observation of chemical fragment information for molecular complexities is a major advantage of biological NMR analysis. Thus, the development of a novel technique for NMR signal assignment and metabolite identification may offer new possibilities for exploring molecular complexities. We propose a new sig...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.5b00894

    authors： Ito K,Tsutsumi Y,Date Y,Kikuchi J

    更新日期：2016-04-15 00:00:00

  • DprE1 Is a Vulnerable Tuberculosis Drug Target Due to Its Cell Wall Localization.

    abstract：:The flavo-enzyme DprE1 catalyzes a key epimerization step in the decaprenyl-phosphoryl d-arabinose (DPA) pathway, which is essential for mycobacterial cell wall biogenesis and targeted by several new tuberculosis drug candidates. Here, using differential radiolabeling with DPA precursors and high-resolution fluorescen...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.5b00237

    authors： Brecik M,Centárová I,Mukherjee R,Kolly GS,Huszár S,Bobovská A,Kilacsková E,Mokošová V,Svetlíková Z,Šarkan M,Neres J,Korduláková J,Cole ST,Mikušová K

    更新日期：2015-07-17 00:00:00

  • Detecting Secretory Proteins by Acoustic Droplet Ejection in Multiplexed High-Throughput Applications.

    abstract：:Nearly one-third of the encoded proteome is comprised of secretory proteins that enable communication between cells and organ systems, playing a ubiquitous role in human health and disease. High-throughput detection of secreted proteins would enhance efforts to identify therapies for secretion-related diseases. Using ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00001

    authors： Iannotti MJ,MacArthur R,Jones R,Tao D,Singeç I,Michael S,Inglese J

    更新日期：2019-03-15 00:00:00

  • Engineering Atypical Tetracycline Formation in Amycolatopsis sulphurea for the Production of Modified Chelocardin Antibiotics.

    abstract：:To combat the increasing spread of antimicrobial resistance and the shortage of novel anti-infectives, one strategy for the development of new antibiotics is to optimize known chemical scaffolds. Here, we focus on the biosynthetic engineering of Amycolatopsis sulphurea for derivatization of the atypical tetracycline c...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.8b01125

    authors： Lukežič T,Fayad AA,Bader C,Harmrolfs K,Bartuli J,Groß S,Lešnik U,Hennessen F,Herrmann J,Pikl Š,Petković H,Müller R

    更新日期：2019-03-15 00:00:00

  • Surface labeling of enveloped viruses assisted by host cells.

    abstract：:Labeling of virus opens new pathways for the understanding of viruses themselves and facilitates the utilization of viruses in modern biology, medicine, and materials. Based on the characteristic that viruses hijack their host cellular machineries to survive and reproduce themselves, a host-cell-assisted strategy is p...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb2001878

    authors： Huang BH,Lin Y,Zhang ZL,Zhuan F,Liu AA,Xie M,Tian ZQ,Zhang Z,Wang H,Pang DW

    更新日期：2012-04-20 00:00:00

  • Structural and Functional Evidence Indicates Selective Oxygen Signaling in Caldanaerobacter subterraneus H-NOX.

    abstract：:Acute and specific sensing of diatomic gas molecules is an essential facet of biological signaling. Heme nitric oxide/oxygen binding (H-NOX) proteins are a family of gas sensors found in diverse classes of bacteria and eukaryotes. The most commonly characterized bacterial H-NOX domains are from facultative anaerobes a...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.6b00431

    authors： Hespen CW,Bruegger JJ,Phillips-Piro CM,Marletta MA

    更新日期：2016-08-19 00:00:00

  • The N-Terminal Domain of NPHP1 Folds into a Monomeric Left-Handed Antiparallel Three-Stranded Coiled Coil with Anti-apoptotic Function.

    abstract：:Mutations in the NPHP1 gene, coding for human nephrocystin-1 (NPHP1), cause the autosomal recessive disease nephronophthisis, the most common cause of end-stage renal disease in children and adolescents. The function and structure of NPHP1 are still poorly characterized. NPHP1 presents a modular structure well in keep...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00582

    authors： Mannella V,Quilici G,Nigro EA,Lampis M,Minici C,Degano M,Boletta A,Musco G

    更新日期：2019-08-16 00:00:00

  • Small Molecule Inhibitors of Human DNA Polymerase λ.

    abstract：:To discover chemical probes to further under-stand the function of individual DNA polymerases, we established a generally applicable high-throughput screening. By applying this technique we discovered three novel inhibitor classes of human DNA polymerase λ (DNA Pol λ), a key enzyme to maintain the genetic integrity of...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb100382m

    authors： Strittmatter T,Bareth B,Immel TA,Huhn T,Mayer TU,Marx A

    更新日期：2011-04-15 00:00:00

  • Biological matching of chemical reactivity: pairing indole nucleophilicity with electrophilic isoprenoids.

    abstract：:The indole side chain of tryptophan has latent nucleophilic reactivity at both N1 and all six (nonbridgehead) carbons, which is not generally manifested in post-translational reactions of proteins. On the other hand, all seven positions can be prenylated by the primary metabolite Δ(2)-isopentenyl diphosphate by dimeth...

    journal_title：ACS chemical biology

    pub_type： 杂志文章,评审

    doi：10.1021/cb500695k

    authors： Walsh CT

    更新日期：2014-12-19 00:00:00

  • Targeting Regorafenib-Induced Toxicity through Inhibition of Gut Microbial β-Glucuronidases.

    abstract：:Regorafenib (Stivarga) is an oral small molecule kinase inhibitor used to treat metastatic colorectal cancer, hepatocellular carcinomas, and gastrointestinal stromal tumors. Diarrhea is one of the most frequently observed adverse reactions associated with regorafenib. This toxicity may arise from the reactivation of t...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.9b00663

    authors： Ervin SM,Hanley RP,Lim L,Walton WG,Pearce KH,Bhatt AP,James LI,Redinbo MR

    更新日期：2019-12-20 00:00:00

  • Peptide-based investigation of the Escherichia coli RNA polymerase σ(70):core interface as target site.

    abstract：:The number of bacterial strains that are resistant against antibiotics increased dramatically during the past decades. This fact stresses the urgent need for the development of new antibacterial agents with novel modes of action targeting essential enzymes such as RNA polymerase (RNAP). Bacterial RNAP is a large multi...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb3005758

    authors： Hüsecken K,Negri M,Fruth M,Boettcher S,Hartmann RW,Haupenthal J

    更新日期：2013-04-19 00:00:00

  • Discovery of a nitric oxide responsive quorum sensing circuit in Vibrio harveyi.

    abstract：:Bacteria use small molecules to assess the density and identity of nearby organisms and formulate a response. This process, called quorum sensing (QS), commonly regulates bioluminescence, biofilm formation, and virulence. Vibrio harveyi have three described QS circuits. Each involves the synthesis of a molecule that r...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb300215t

    authors： Henares BM,Higgins KE,Boon EM

    更新日期：2012-08-17 00:00:00

  • Mechanistic Insights into Taxadiene Epoxidation by Taxadiene-5α-Hydroxylase.

    abstract：:The anticancer molecule taxol (Paclitaxel) stands as one of the most medically and economically important natural products. However, despite decades of extensive study, its biosynthesis remains poorly understood. Unpredictable behavior of the first oxygenation enzyme, taxadiene-5α-hydroxylase, which produces a range o...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.5b00767

    authors： Edgar S,Zhou K,Qiao K,King JR,Simpson JH,Stephanopoulos G

    更新日期：2016-02-19 00:00:00

  • Small-molecule screening: advances in microarraying and cell-imaging technologies.

    abstract：:Cell-permeable small molecules can be used to modulate protein function selectively, rapidly, reversibly, and conditionally with temporal and quantitative control in biological systems. The identification of these chemical probes can require the screening of large numbers of small molecules. With the advent of new tec...

    journal_title：ACS chemical biology

    pub_type： 杂志文章,评审

    doi：10.1021/cb600321j

    authors： Nicholson RL,Welch M,Ladlow M,Spring DR

    更新日期：2007-01-23 00:00:00

  • Covalent-Fragment Screening of BRD4 Identifies a Ligandable Site Orthogonal to the Acetyl-Lysine Binding Sites.

    abstract：:BRD4, a member of the bromodomain and extraterminal domain (BET) family, has emerged as a promising epigenetic target in cancer and inflammatory disorders. All reported BET family ligands bind within the bromodomain acetyl-lysine binding sites and competitively inhibit BET protein interaction with acetylated chromatin...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00058

    authors： Olp MD,Sprague DJ,Goetz CJ,Kathman SG,Wynia-Smith SL,Shishodia S,Summers SB,Xu Z,Statsyuk AV,Smith BC

    更新日期：2020-04-17 00:00:00

  • Measuring picomolar intracellular exchangeable zinc in PC-12 cells using a ratiometric fluorescence biosensor.

    abstract：:Zinc plays both physiological and pathological roles in biology, making it of increasing interest. To date, intracellular free zinc has been measured in cell types supplemented with or enriched in zinc, such as hippocampal neurons. Here we quantitatively image intracellular exchangeable zinc in an ordinary resting cel...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb500043a

    authors： Bozym RA,Thompson RB,Stoddard AK,Fierke CA

    更新日期：2006-03-17 00:00:00

  • Heavy Metals Induce Decline of Derivatives of 5-Methycytosine in Both DNA and RNA of Stem Cells.

    abstract：:Toxic heavy metals have been considered to be harmful environmental contaminations. The molecular mechanisms of heavy-metals-induced cytotoxicity and carcinogenicity are still not well elucidated. Previous reports showed exposures to toxic heavy metals can cause a change of DNA cytosine methylation (5-methylcytosine, ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.7b00170

    authors： Xiong J,Liu X,Cheng QY,Xiao S,Xia LX,Yuan BF,Feng YQ

    更新日期：2017-06-16 00:00:00

  • Insertions within the Saxitoxin Biosynthetic Gene Cluster Result in Differential Toxin Profiles.

    abstract：:The neurotoxin saxitoxin and related paralytic shellfish toxins are produced by multiple species of cyanobacteria and dinoflagellates. This study investigates the two saxitoxin-producing strains of Scytonema crispum, CAWBG524 and CAWBG72, isolated in New Zealand. Each strain was previously reported to have a distinct ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.8b00608

    authors： Cullen A,D'Agostino PM,Mazmouz R,Pickford R,Wood S,Neilan BA

    更新日期：2018-11-16 00:00:00

  • Functional evaluation of key interactions evident in the structure of the eukaryotic Cys-loop receptor GluCl.

    abstract：:The publication of the first high-resolution crystal structure of a eukaryotic Cys-loop receptor, GluClα, has provided valuable structural information on this important class of ligand-gated ion channels (LGIC). However, limited functional data exist for the GluCl receptors. Before applying the structural insights fro...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/cb500323d

    authors： Daeffler KN,Lester HA,Dougherty DA

    更新日期：2014-10-17 00:00:00

  • Cell-Penetrating Peptides Escape the Endosome by Inducing Vesicle Budding and Collapse.

    abstract：:Cell-penetrating peptides (CPPs) are capable of delivering membrane-impermeable cargoes (including small molecules, peptides, proteins, nucleic acids, and nanoparticles) into the cytosol of mammalian cells and have the potential to revolutionize biomedical research and drug discovery. However, the mechanism of action ...

    journal_title：ACS chemical biology

    pub_type： 杂志文章

    doi：10.1021/acschembio.0c00478

    authors： Sahni A,Qian Z,Pei D

    更新日期：2020-09-18 00:00:00