Abstract：
:Bacteria use small molecules to assess the density and identity of nearby organisms and formulate a response. This process, called quorum sensing (QS), commonly regulates bioluminescence, biofilm formation, and virulence. Vibrio harveyi have three described QS circuits. Each involves the synthesis of a molecule that regulates phosphorylation of its cognate receptor kinase. Each receptor exchanges phosphate with a common phosphorelay protein, LuxU, which ultimately regulates bioluminescence. Here, we show that another small molecule, nitric oxide (NO), participates in QS through LuxU. V. harveyi display a NO concentration-dependent increase in bioluminescence that is regulated by an hnoX gene. We demonstrate that H-NOX is a NO sensor and NO/H-NOX regulates phosphorylation of a kinase that transfers phosphate to LuxU. This study reveals the discovery of a fourth QS pathway in V. harveyi and suggests that bacteria use QS to integrate not only the density of bacteria but also other diverse information about their environment into decisions about gene expression.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Henares BM,Higgins KE,Boon EMdoi
10.1021/cb300215tsubject
Has Abstractpub_date
2012-08-17 00:00:00pages
1331-6issue
8eissn
1554-8929issn
1554-8937journal_volume
7pub_type
杂志文章abstract：:Chromophore-assisted light inactivation (CALI) is a potentially powerful tool for the acute disruption of a target protein inside living cells with high spatiotemporal resolution. This technology, however, has not been widely utilized, mainly because of the lack of an efficient chromophore as the photosensitizing agen...
journal_title：ACS chemical biology
pub_type： 信件
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更新日期：2011-05-20 00:00:00
abstract：:There is a current and pressing need for improved cancer therapies. The use of small molecule kinase inhibitors and their application in combinatorial regimens represent an approach to personalized targeted cancer therapy. A number of AGC kinases, including atypical Protein Kinase C enzymes (PKCs), are validated drug ...
journal_title：ACS chemical biology
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journal_title：ACS chemical biology
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更新日期：2007-08-17 00:00:00
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journal_title：ACS chemical biology
pub_type： 杂志文章
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更新日期：2018-09-21 00:00:00
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journal_title：ACS chemical biology
pub_type： 杂志文章
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更新日期：2019-04-19 00:00:00
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更新日期：2015-06-19 00:00:00
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更新日期：2006-11-21 00:00:00
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pub_type： 评论,杂志文章
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更新日期：2010-06-18 00:00:00
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更新日期：2014-02-21 00:00:00
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更新日期：2012-07-20 00:00:00
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更新日期：2013-12-20 00:00:00
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更新日期：2013-07-19 00:00:00
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