Design and characterization of an active site selective caspase-3 transnitrosating agent.

Abstract:

:The oxidative addition of nitric oxide (NO) to a thiol, S-nitrosation, is a focus of studies on cyclic guanosine monophosphate (cGMP)-independent NO signaling. S-Nitrosation of the catalytic cysteine of the caspase proteases has important effects on apoptosis and consequently has received attention. Here we report on a small molecule that can directly probe the effects of S-nitrosation on the caspase cascade. This chemical tool is capable of permeating the mammalian cell membrane, selectively transnitrosating the caspase-3 active site cysteine, and halting apoptosis in cultured human T-cells. The efficacy of this reagent was compared with the commonly used reagent S-nitrosoglutathione and an esterified derivative.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Mitchell DA,Morton SU,Marletta MA

doi

10.1021/cb600393x

subject

Has Abstract

pub_date

2006-11-21 00:00:00

pages

659-65

issue

10

eissn

1554-8929

issn

1554-8937

journal_volume

1

pub_type

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