Abstract:
:The oxidative addition of nitric oxide (NO) to a thiol, S-nitrosation, is a focus of studies on cyclic guanosine monophosphate (cGMP)-independent NO signaling. S-Nitrosation of the catalytic cysteine of the caspase proteases has important effects on apoptosis and consequently has received attention. Here we report on a small molecule that can directly probe the effects of S-nitrosation on the caspase cascade. This chemical tool is capable of permeating the mammalian cell membrane, selectively transnitrosating the caspase-3 active site cysteine, and halting apoptosis in cultured human T-cells. The efficacy of this reagent was compared with the commonly used reagent S-nitrosoglutathione and an esterified derivative.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Mitchell DA,Morton SU,Marletta MAdoi
10.1021/cb600393xsubject
Has Abstractpub_date
2006-11-21 00:00:00pages
659-65issue
10eissn
1554-8929issn
1554-8937journal_volume
1pub_type
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