Abstract：
:Hemolysis, a process by which the destruction of red blood cells leads to the release of hemoglobin, is a critical event observed during hemolytic disorders. Under oxidative stress conditions, hemoglobin can release its heme prosthetic group, which is highly cytotoxic and can catalyze the generation of reactive oxygen species (ROS), leading to several undesired redox reactions in the cells. Herein, we demonstrate for the first time that heme can mediate the activation and death of human platelets through ferroptosis, which is an iron-dependent form of nonapoptotic cell death. This study also suggests that the heme-mediated lipid peroxidation and ferroptosis in platelets may play an important role in hemolytic disorders.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
NaveenKumar SK,SharathBabu BN,Hemshekhar M,Kemparaju K,Girish KS,Mugesh Gdoi
10.1021/acschembio.8b00458subject
Has Abstractpub_date
2018-08-17 00:00:00pages
1996-2002issue
8eissn
1554-8929issn
1554-8937journal_volume
13pub_type
杂志文章abstract：:Protein phosphatases are critical regulators of cellular signaling in both eukaryotes and prokaryotes. The majority of protein phosphatases dephosphorylate phosphoserine/phosphothreonine or phosphotyrosine residues. Recently, however, YwlE, a member of the low-molecular weight protein tyrosine phosphatase (LMW-PTP) fa...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb4001469
更新日期：2013-09-20 00:00:00
abstract：:We have developed a chemically controlled very long-acting delivery system to support once-monthly administration of a peptidic GLP-1R agonist. Initially, the prototypical GLP-1R agonist exenatide was covalently attached to hydrogel microspheres by a self-cleaving β-eliminative linker; after subcutaneous injection in ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00218
更新日期：2017-08-18 00:00:00
abstract：:The innate immune system provides the first line of defense against pathogens through the recognition of nonspecific patterns in RNA to protect the cell in a generalized way. The human RNA-activated protein kinase, PKR, is a dsRNA binding protein and an essential sensor in the innate immune response, which recognizes ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00081
更新日期：2016-04-15 00:00:00
abstract：:A complication of diabetes is the inability of wounds to heal in diabetic patients. Diabetic wounds are refractory to healing due to the involvement of activated matrix metalloproteinases (MMPs), which remodel the tissue resulting in apoptosis. There are no readily available methods that identify active unregulated MM...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb4005468
更新日期：2014-01-17 00:00:00
abstract：:Thermodynamics and kinetics of protein-ligand binding are both important aspects for the design of novel drug molecules. Presently, thermodynamic data are collected with isothermal titration calorimetry, while kinetic data are mostly derived from surface plasmon resonance. The new method of kinITC provides both thermo...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00895
更新日期：2020-03-20 00:00:00
abstract：:Dicamba monooxygenase (DMO) catalyzes the O-demethylation of dicamba (3,6-dichloro-2-methoxybenzoate) to produce 3,6-dichlorosalicylate and formaldehyde. Recent crystallographic studies suggest that DMO catalyzes the challenging oxidation of a saturated C-H bond within the methyl group of dicamba to form a hemiacetal ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400154a
更新日期：2013-08-16 00:00:00
abstract：:Drug resistance continues to be a growing global problem. The efficacy of small molecule inhibitors is threatened by pools of genetic diversity in all systems, including antibacterials, antifungals, cancer therapeutics, and antivirals. Resistant variants often include combinations of active site mutations and distal "...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00370
更新日期：2019-11-15 00:00:00
abstract：:Inducible modulation is often required for precise investigations and manipulations of dynamic biological processes. Transcription activator-like effectors (TALEs) provide a powerful tool for targeted gene editing and transcriptional programming. We designed a series of chemical inducible systems by coupling TALEs wit...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00606
更新日期：2018-03-16 00:00:00
abstract：:The receptor tyrosine kinase EPHA2 (Ephrin type-A receptor 2) plays important roles in oncogenesis, metastasis, and treatment resistance, yet therapeutic targeting, drug discovery, or investigation of EPHA2 biology is hampered by the lack of appropriate inhibitors and structural information. Here, we used chemical pro...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00709
更新日期：2016-12-16 00:00:00
abstract：:Flavin-containing monooxygenases (FMOs) are emerging as effective players in oxidative drug metabolism. Until recently, the functions of the five human FMO isoforms were mostly linked to their capability of oxygenating molecules containing soft N- and S-nucleophiles. However, the human FMO isoform 5 was recently shown...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00470
更新日期：2017-09-15 00:00:00
abstract：:Next-generation DNA sequencing technologies have led to a massive accumulation of genomic and transcriptomic data from patients and healthy individuals. The major challenge ahead is to understand the functional significance of the elements of the human genome and transcriptome, and implications for diagnosis and treat...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/acschembio.7b00657
更新日期：2018-02-16 00:00:00
abstract：:We recently reported two novel tools for precisely controlling and quantifying Cas9 activity: a chemically inducible Cas9 variant (ciCas9) that can be rapidly activated by small molecules and a ddPCR assay for time-resolved measurement of DNA double strand breaks (DSB-ddPCR). Here, we further demonstrate the potential...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00652
更新日期：2018-02-16 00:00:00
abstract：:A major goal of personalized medicine in oncology is the identification of drugs with predictable efficacy based on a specific trait of the cancer cell, as has been demonstrated with gleevec (presence of Bcr-Abl protein), herceptin (Her2 overexpression), and iressa (presence of a specific EGFR mutation). This is a cha...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb4005832
更新日期：2013-10-18 00:00:00
abstract：:The human ClpP proteolytic complex (HsClpP) is a serine protease located in the mitochondrial matrix and participates in the maintenance of the mitochondrial proteome among other cellular functions. HsClpP typically forms a multimeric complex with the AAA+ protein unfoldase HsClpX. Notably, compared to that of normal,...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/acschembio.9b00347
更新日期：2019-11-15 00:00:00
abstract：:Reactive oxygen species (ROS) play an important role in the onset of Parkinson's disease (PD), and deciphering protective mechanisms is a major goal for therapeutic development. Here, DJ-1 (PARK7) gained major attention when a conserved cysteine residue with a putative role in oxidative stress sensing/protection was l...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00633
更新日期：2018-08-17 00:00:00
abstract：:Development of precision therapeutics is of immense interest, particularly as applied to the treatment of cancer. By analyzing the preferred cellular RNA targets of small molecules, we discovered that 5"-azido neomycin B binds the Drosha processing site in the microRNA (miR)-525 precursor. MiR-525 confers invasive pro...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00615
更新日期：2016-02-19 00:00:00
abstract：:RNA modifications play important roles in RNA structures and regulation of gene expression and translation. We report the first RNA modification on the phosphate, the RNA phosphorothioate (PS) modification, discovered in both prokaryotes and eukaryotes. The PS modification is also first reported on nucleic acids of eu...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00163
更新日期：2020-06-19 00:00:00
abstract：:Bacterial tRNA-guanine transglycosylase (Tgt) is involved in the biosynthesis of the modified tRNA nucleoside queuosine present in the anticodon wobble position of tRNAs specific for aspartate, asparagine, histidine, and tyrosine. Inactivation of the tgt gene leads to decreased pathogenicity of Shigella bacteria. Ther...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00700
更新日期：2020-11-20 00:00:00
abstract：:Nearly one-third of the encoded proteome is comprised of secretory proteins that enable communication between cells and organ systems, playing a ubiquitous role in human health and disease. High-throughput detection of secreted proteins would enhance efforts to identify therapies for secretion-related diseases. Using ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00001
更新日期：2019-03-15 00:00:00
abstract：:MbtA is an adenylating enzyme from Mycobacterium tuberculosis that catalyzes the first step in the biosynthesis of the mycobactins. A bisubstrate inhibitor of MbtA (Sal-AMS) was previously described that displays potent antitubercular activity under iron-replete as well as iron-deficient growth conditions. This findin...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300112x
更新日期：2012-10-19 00:00:00
abstract：:Small molecule kinase inhibitors that stabilize distinct ATP binding site conformations can differentially modulate the global conformation of Src-family kinases (SFKs). However, it is unclear which specific ATP binding site contacts are responsible for modulating the global conformation of SFKs and whether these inhi...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00429
更新日期：2020-07-17 00:00:00
abstract：:The nematode Caenorhabditis elegans is an excellent model organism for studies of glycan dynamics, a goal that requires tools for imaging glycans in vivo. Here we applied the bioorthogonal chemical reporter technique for the molecular imaging of mucin-type O-glycans in live C. elegans. We treated worms with azidosugar...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb900254y
更新日期：2009-12-18 00:00:00
abstract：:The Siglec family of sialic acid-binding proteins are differentially expressed on white blood cells of the immune system and represent an attractive class of targets for cell-directed therapy. Nanoparticles decorated with high-affinity Siglec ligands show promise for delivering cargo to Siglec-bearing cells, but this ...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/cb400125w
更新日期：2013-07-19 00:00:00
abstract：:A new chemical method for the traceless labeling of glycoproteins with synthetic boronic acid (BA)-tosyl probes was successfully developed. The BA moiety acts as an affinity head to direct the formation of a cyclic boronate diester with the diol groups of glycans. Following this step, the electrophilic tosyl group is ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400631w
更新日期：2014-02-21 00:00:00
abstract：:Mutations that constitutively activate the phosphatidyl-inositol-3-kinase (PI3K) signaling pathway, including alterations in PI3K, PTEN, and AKT, are found in a variety of human cancers, implicating the PI3K lipid kinase as an attractive target for the development of therapeutic agents to treat cancer and other relate...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb800039y
更新日期：2008-05-16 00:00:00
abstract：:Engineering gene circuits with novel functions holds promise for broad applications in biology, engineering, and medicine. Directed evolution complements rational design as an important strategy for optimizing gene circuits and circuit elements. ...
journal_title：ACS chemical biology
pub_type： 评论,杂志文章,评审
doi：10.1021/cb6004596
更新日期：2006-12-20 00:00:00
abstract：:Synthetic compounds for controlling or creating human immunity have the potential to revolutionize disease treatment. Motivated by challenges in this arena, we report herein a strategy to target metastatic cancer cells for immune-mediated destruction by targeting the urokinase-type plasminogen activator receptor (uPAR...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb200374e
更新日期：2012-02-17 00:00:00
abstract：:Endothelial progenitor cells (EPCs) and endothelial cells (ECs) play a vital role in endothelialization and vascularization for tissue regeneration. Various EPC/EC targeting biomolecules have been investigated to improve tissue regeneration with limited success often due to their limited functional specificity and str...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00118
更新日期：2017-04-21 00:00:00
abstract：:Although peptide-based reporters of protein tyrosine kinase (PTK) activity have been used to study PTK enzymology in vitro, the application of these reporters to intracellular conditions is compromised by their dephosphorylation, preventing PTK activity measurements. Nonproteinogenic amino acids may be utilized to rat...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00667
更新日期：2016-02-19 00:00:00
abstract：:Platinum-based drugs have been used to successfully treat diverse cancers for several decades. Cisplatin, the original compound of this class, cross-links DNA, resulting in cell cycle arrest and cell death via apoptosis. Cisplatin is effective against several tumor types, yet it exhibits toxic side effects and tumors ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300320d
更新日期：2012-11-16 00:00:00
