Abstract:
:RNA modifications play important roles in RNA structures and regulation of gene expression and translation. We report the first RNA modification on the phosphate, the RNA phosphorothioate (PS) modification, discovered in both prokaryotes and eukaryotes. The PS modification is also first reported on nucleic acids of eukaryotes. The GpsG modification exists in the Rp configuration and was quantified with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). By knocking out the DndA gene in E. coli, we show the Dnd clusters that regulate DNA PS modification may also play roles in RNA PS modification. We also show that the GpsG modification locates on rRNA in E. coli, L. lactis, and HeLa cells, and it is not detected in rRNA-depleted total RNAs from these cells.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Wu Y,Tang Y,Dong X,Zheng YY,Haruehanroengra P,Mao S,Lin Q,Sheng Jdoi
10.1021/acschembio.0c00163subject
Has Abstractpub_date
2020-06-19 00:00:00pages
1301-1305issue
6eissn
1554-8929issn
1554-8937journal_volume
15pub_type
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