Abstract:
:Many cellular processes are regulated by posttranslational modifications that are recognized by specific domains in protein binding partners. These interactions are often weak, thus allowing a highly dynamic and combinatorial regulatory network of protein-protein interactions. We report an efficient strategy that overcomes challenges in structural analysis of such a weak transient interaction between the Tudor domain of the Survival of Motor Neuron (SMN) protein and symmetrically dimethylated arginine (sDMA). The posttranslational modification is chemically introduced and covalently linked to the effector module by a one-pot expressed protein ligation (EPL) procedure also enabling segmental incorporation of NMR-active isotopes for structural analysis. Covalent coupling of the two interacting moieties shifts the equilibrium to the bound state, and stoichiometric interactions are formed even for low affinity interactions. Our approach should enable the structural analysis of weak interactions by NMR or X-ray crystallography to better understand the role of posttranslational modifications in dynamic biological processes.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Tripsianes K,Chu NK,Friberg A,Sattler M,Becker CFdoi
10.1021/cb400723jsubject
Has Abstractpub_date
2014-02-21 00:00:00pages
347-52issue
2eissn
1554-8929issn
1554-8937journal_volume
9pub_type
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