Approaches to Study Phosphatases.

Abstract:

:Phosphatases play key roles in normal physiology and diseases. Studying phosphatases has been both essential and challenging, and the application of conventional genetic and biochemical methods has led to crucial but still limited understanding of their mechanisms, substrates, and exclusive functions within highly intricate networks. With the advances in technologies such as cellular imaging and molecular and chemical biology in terms of sensitive tools and methods, the phosphatase field has thrived in the past years and has set new insights for cell signaling studies and for therapeutic development. In this review, we give an overview of the existing interdisciplinary tools for phosphatases, give examples on how they have been applied to increase our understanding of these enzymes, and suggest how they-and other tools yet barely used in the phosphatase field-might be adapted to address future questions and challenges.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Fahs S,Lujan P,Köhn M

doi

10.1021/acschembio.6b00570

subject

Has Abstract

pub_date

2016-11-18 00:00:00

pages

2944-2961

issue

11

eissn

1554-8929

issn

1554-8937

journal_volume

11

pub_type

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