Structure-Based Mutagenesis of Phycobiliprotein smURFP for Optoacoustic Imaging.

Abstract:

:Photo- or optoacoustics (OA) imaging is increasingly being used as a non-invasive imaging method that can simultaneously reveal structure and function in deep tissue. However, the most frequent transgenic OA labels are current fluorescent proteins that are not optimized for OA imaging. Thus, they lack OA signal strength, and their absorption maxima are positioned at short wavelengths, thus giving small penetration depths and strong background signals. Here, we apply insights from our recent determination of the structure of the fluorescent phycobiliprotein smURFP to mutate a range of residues to promote the nonradiative decay pathway that generates the OA signal. We identified hydrophobic and aromatic substitutions within the chromophore-binding pocket that substantially increase the intensity of the OA signal and red-shift the absorption. Our results demonstrate the feasibility of structure-based mutagenesis to repurpose fluorescent probes for OA imaging, and they may provide structure-function insights for de novo engineering of transgenic OA probes.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Fuenzalida Werner JP,Mishra K,Huang Y,Vetschera P,Glasl S,Chmyrov A,Richter K,Ntziachristos V,Stiel AC

doi

10.1021/acschembio.9b00299

subject

Has Abstract

pub_date

2019-09-20 00:00:00

pages

1896-1903

issue

9

eissn

1554-8929

issn

1554-8937

journal_volume

14

pub_type

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