Abstract:
:Photo- or optoacoustics (OA) imaging is increasingly being used as a non-invasive imaging method that can simultaneously reveal structure and function in deep tissue. However, the most frequent transgenic OA labels are current fluorescent proteins that are not optimized for OA imaging. Thus, they lack OA signal strength, and their absorption maxima are positioned at short wavelengths, thus giving small penetration depths and strong background signals. Here, we apply insights from our recent determination of the structure of the fluorescent phycobiliprotein smURFP to mutate a range of residues to promote the nonradiative decay pathway that generates the OA signal. We identified hydrophobic and aromatic substitutions within the chromophore-binding pocket that substantially increase the intensity of the OA signal and red-shift the absorption. Our results demonstrate the feasibility of structure-based mutagenesis to repurpose fluorescent probes for OA imaging, and they may provide structure-function insights for de novo engineering of transgenic OA probes.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Fuenzalida Werner JP,Mishra K,Huang Y,Vetschera P,Glasl S,Chmyrov A,Richter K,Ntziachristos V,Stiel ACdoi
10.1021/acschembio.9b00299subject
Has Abstractpub_date
2019-09-20 00:00:00pages
1896-1903issue
9eissn
1554-8929issn
1554-8937journal_volume
14pub_type
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