Abstract：
:Pathogenic Yersinia spp. secrete the effector YopJ (YopP) into host cells to counteract cytokine production and to induce programmed cell death (apoptosis). YopJ achieves these aims by inactivating mitogen-activated protein kinase (MAPK) and nuclear factor kappaB signaling pathways. YopJ was shown to bind to members of the MAPK kinase (MKK) family and was predicted to have protease activity toward ubiquitin (Ub)-like proteins. In a recent report, YopJ was demonstrated to inactivate MKKs via acetylation of critical serine or threonine residues. The ramifications of these exciting results are discussed in the context of other studies implicating YopJ as a Ub-like protease.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Bliska JBdoi
10.1021/cb600261ksubject
Has Abstractpub_date
2006-07-21 00:00:00pages
349-51issue
6eissn
1554-8929issn
1554-8937journal_volume
1pub_type
杂志文章,评审abstract：:The perturbation of protein kinases with small organic molecules is a powerful approach to dissect kinase function in complex biological systems. Covalent kinase inhibitors that target thiols in the ATP binding pocket of the kinase domain proved to be ideal reagents for the investigation of highly dynamic cellular pro...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb200465c
更新日期：2012-04-20 00:00:00
abstract：:Development of precision therapeutics is of immense interest, particularly as applied to the treatment of cancer. By analyzing the preferred cellular RNA targets of small molecules, we discovered that 5"-azido neomycin B binds the Drosha processing site in the microRNA (miR)-525 precursor. MiR-525 confers invasive pro...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00615
更新日期：2016-02-19 00:00:00
abstract：:Synthetic biology is the realization of systems with desired behavior using biological materials. A recent addition to the field is a bipartite consortium of the bacterium Escherichia coli in which each species harbors complementary gene circuits that actuate only when both are present above a critical density. This b...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb700256w
更新日期：2008-01-18 00:00:00
abstract：:Ubiquitin specific protease 7 (USP7) regulates the protein stability of key cellular regulators in pathways ranging from apoptosis to neuronal development, making it a promising therapeutic target. Here we used an engineered, activated variant of the USP7 catalytic domain to perform structure-activity studies of elect...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00031
更新日期：2020-06-19 00:00:00
abstract：:Simocyclinone D8 (1, SD8) has attracted attention due to its highly complex hybrid structure and the unusual way it inhibits bacterial DNA gyrase by preventing DNA binding to the enzyme. Although a hypothesis explaining simocyclinone biosynthesis has been previously proposed, little was proven in vivo due to the genet...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00669
更新日期：2016-01-15 00:00:00
abstract：:Understanding the complex biochemical mechanisms that underlie the regulation, toxicity, and protein binding of metal ions requires the ability to analyze the metal content of individual proteins in complex mixtures. In this issue of ACS Chemical Biology, a technique combining gel electrophoresis with synchrotron X-ra...
journal_title：ACS chemical biology
pub_type： 评论,杂志文章
doi：10.1021/cb100145z
更新日期：2010-06-18 00:00:00
abstract：:Synthetic compounds for controlling or creating human immunity have the potential to revolutionize disease treatment. Motivated by challenges in this arena, we report herein a strategy to target metastatic cancer cells for immune-mediated destruction by targeting the urokinase-type plasminogen activator receptor (uPAR...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb200374e
更新日期：2012-02-17 00:00:00
abstract：:Epitranscriptomic modifications play an important role in RNA function and can impact gene expression. Here, we apply a chemical proteomics approach to investigate readers of N1-methyladenosine (m1A), a poorly characterized modification on mammalian mRNA. We find that YTHDF proteins, known m6A readers, recognize m1A-m...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00655
更新日期：2020-01-17 00:00:00
abstract：:The LpxC enzyme in the lipid A biosynthetic pathway is one of the most promising and clinically unexploited antibiotic targets for treatment of multidrug-resistant Gram-negative infections. Progress in medicinal chemistry has led to the discovery of potent LpxC inhibitors with a variety of chemical scaffolds and disti...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400067g
更新日期：2014-01-17 00:00:00
abstract：:Glycosylphosphatidylinositol (GPI)-anchored proteins are abundant in the protozoan parasite Trypanosoma brucei, the causative agent of African sleeping sickness in humans and the related disease Nagana in cattle, and disruption of GPI biosynthesis is genetically and chemically validated as a drug target. Here, we exam...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb800143w
更新日期：2008-10-17 00:00:00
abstract：:Replacing the two Mn(2+) ions normally present in human Arginase I with Co(2+) resulted in a significantly lowered K(M) value without a concomitant reduction in k(cat). In addition, the pH dependence of the reaction was shifted from a pK(a) of 8.5 to a pK(a) of 7.5. The combination of these effects led to a 10-fold in...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb900267j
更新日期：2010-03-19 00:00:00
abstract：:The nonheme diiron enzyme cyanobacterial aldehyde deformylating oxygenase, cADO, catalyzes the highly unusual deformylation of aliphatic aldehydes to alkanes and formate. We have determined crystal structures for the enzyme with a long-chain water-soluble aldehyde and medium-chain carboxylic acid bound to the active s...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb500343j
更新日期：2014-11-21 00:00:00
abstract：:Regulators of G protein signaling (RGS) proteins are key players in regulating signaling via G protein-coupled receptors. RGS proteins directly bind to the Gα-subunits of activated heterotrimeric G-proteins, and accelerate the rate of GTP hydrolysis, thereby rapidly deactivating G-proteins. Using atomistic simulations...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400568g
更新日期：2013-12-20 00:00:00
abstract：:Reporter gene assays (RGAs) are commonly used to measure biological pathway modulation by small molecules. Understanding how such compounds interact with the reporter enzyme is critical to accurately interpret RGA results. To improve our understanding of reporter enzymes and to develop optimal RGA systems, we investig...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb3007264
更新日期：2013-05-17 00:00:00
abstract：:Ca(II) ions are critical for the proper function of neurons by contributing to synaptic signaling and regulating neuronal plasticity. Dysregulation of Ca(II) is associated with a number of pathologies that cause neurodegeneration; therefore the ability to monitor Ca(II) intracellularly is an important target for molec...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00638
更新日期：2020-02-21 00:00:00
abstract：:Hydrogen sulfide (H2S) is an important gasotransmitter and biomolecule, and many synthetic small-molecule H2S donors have been developed for H2S-related research. One important class of triggerable H2S donors is self-immolative thiocarbamates, which function by releasing carbonyl sulfide (COS), which is rapidly conver...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/acschembio.8b00981
更新日期：2019-02-15 00:00:00
abstract：:The emergence of multidrug-resistant Staphylococcus aureus strains has become a serious clinical problem. Iron is absolutely required for the bacterial growth, virulence associated with colonization, and survival from the host immune system. The FeoB protein is a major iron permease in bacterial ferrous iron transport...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00842
更新日期：2021-01-15 00:00:00
abstract：:The steady increase in the prevalence of multidrug-resistant Staphylococcus aureus has made the search for novel antibiotics to combat this clinically important pathogen an urgent matter. In an effort to discover antibacterials with new chemical structures and mechanisms, we performed a growth inhibition screen of a s...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00982
更新日期：2020-07-17 00:00:00
abstract：:Enzymatic transfer of the AMP portion of ATP to substrate proteins has recently been described as an essential mechanism of bacterial infection for several pathogens. The first AMPylator to be discovered, VopS from Vibrio parahemolyticus, catalyzes the transfer of AMP onto the host GTPases Cdc42 and Rac1. Modification...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb4006886
更新日期：2014-02-21 00:00:00
abstract：:Kinetic parameters of protein-ligand interactions are progressively acknowledged as valuable information for rational drug discovery. However, a targeted optimization of binding kinetics is not easy to achieve, and further systematic studies are necessary to increase the understanding about molecular mechanisms involv...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00979
更新日期：2017-01-20 00:00:00
abstract：:The human ClpP proteolytic complex (HsClpP) is a serine protease located in the mitochondrial matrix and participates in the maintenance of the mitochondrial proteome among other cellular functions. HsClpP typically forms a multimeric complex with the AAA+ protein unfoldase HsClpX. Notably, compared to that of normal,...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/acschembio.9b00347
更新日期：2019-11-15 00:00:00
abstract：:The mitochondrial ADP/ATP carrier (AAC) is a prominent actor in the energetic regulation of the cell, importing ADP into the mitochondria and exporting ATP toward the cytoplasm. Severe genetic diseases have been ascribed to specific mutations in this membrane protein. How minute, well-localized modifications of the tr...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300012j
更新日期：2012-07-20 00:00:00
abstract：:In type-2 diabetes (T2D), islet amyloid polypeptide (IAPP) self-associates into toxic assemblies causing islet β-cell death. Therefore, preventing IAPP toxicity is a promising therapeutic strategy for T2D. The molecular tweezer CLR01 is a supramolecular tool for selective complexation of K residues in (poly)peptides. ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00146
更新日期：2015-06-19 00:00:00
abstract：:We report progress toward a general strategy for mimicking the recognition properties of specific α-helices within natural proteins through the use of oligomers that are less susceptible than conventional peptides to proteolysis. The oligomers contain both α- and β-amino acid residues, with the density of the β subuni...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00109
更新日期：2015-07-17 00:00:00
abstract：:New technology for the derivatization of peptide natural products is required for drug development. Despite the recent advances in the genome sequencing technique enabling us to search for the biosynthetic genes for wide variety of natural products, the technical methods to get access to them are limited. A class of R...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00330
更新日期：2019-06-21 00:00:00
abstract：:The quest for ever more selective kinase inhibitors as potential future drugs has yielded a large repertoire of chemical probes that are selective for specific kinase conformations. These probes have been useful tools to obtain structural snapshots of kinase conformational plasticity. Similarly, kinetic and thermodyna...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb500870a
更新日期：2015-01-16 00:00:00
abstract：:Hemolysis, a process by which the destruction of red blood cells leads to the release of hemoglobin, is a critical event observed during hemolytic disorders. Under oxidative stress conditions, hemoglobin can release its heme prosthetic group, which is highly cytotoxic and can catalyze the generation of reactive oxygen...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00458
更新日期：2018-08-17 00:00:00
abstract：:Chloropupukeananin and chloropestolides are novel metabolites of the plant endophyte Pestalotiopsis fici, showing antimicrobial, antitumor, and anti-HIV activities. Their highly complex and unique skeletons were generated from the coisolated pestheic acid (1) and iso-A82775C (10) based on our previous studies. Here, w...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b01059
更新日期：2018-03-16 00:00:00
abstract：:Several C-β-d-glucopyranosyl azoles have recently been uncovered as among the most potent glycogen phosphorylase (GP) catalytic site inhibitors discovered to date. Toward further exploring their translational potential, ex vivo experiments have been performed for their effectiveness in reduction of glycogenolysis in h...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00172
更新日期：2019-07-19 00:00:00
abstract：:Nephronectin is an extracellular matrix protein that interacts with the α8β1 integrin receptor and plays a role in tissue and organ development, though the motifs that mediate adhesion to the receptor remain unclear. This paper describes the use of self-assembled monolayers to study the adhesion of α8β1-presenting cel...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb200186j
更新日期：2011-10-21 00:00:00
