Abstract:
:Synthetic biology is the realization of systems with desired behavior using biological materials. A recent addition to the field is a bipartite consortium of the bacterium Escherichia coli in which each species harbors complementary gene circuits that actuate only when both are present above a critical density. This bacterial "consensus" system, functional in liquid, solid, and biofilm niches, represents a novel strategy that raises the bar in terms of the specificity and complexity of tasks performed by engineered organisms.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Wong JV,Song H,You Ldoi
10.1021/cb700256wsubject
Has Abstractpub_date
2008-01-18 00:00:00pages
27-9issue
1eissn
1554-8929issn
1554-8937journal_volume
3pub_type
杂志文章abstract::A new chemical method for the traceless labeling of glycoproteins with synthetic boronic acid (BA)-tosyl probes was successfully developed. The BA moiety acts as an affinity head to direct the formation of a cyclic boronate diester with the diol groups of glycans. Following this step, the electrophilic tosyl group is ...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb400631w
更新日期:2014-02-21 00:00:00
abstract::Despite being extensively characterized structurally and biochemically, the functional role of histone deacetylase 8 (HDAC8) has remained largely obscure due in part to a lack of known cellular substrates. Herein, we describe an unbiased approach using chemical tools in conjunction with sophisticated proteomics method...
journal_title:ACS chemical biology
pub_type: 信件
doi:10.1021/cb500492r
更新日期:2014-10-17 00:00:00
abstract::There is a current and pressing need for improved cancer therapies. The use of small molecule kinase inhibitors and their application in combinatorial regimens represent an approach to personalized targeted cancer therapy. A number of AGC kinases, including atypical Protein Kinase C enzymes (PKCs), are validated drug ...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.6b00827
更新日期:2017-02-17 00:00:00
abstract::Transcriptomes provide a myriad of potential RNAs that could be the targets of therapeutics or chemical genetic probes of function. Cell-permeable small molecules, however, generally do not exploit these targets, owing to the difficulty in the design of high affinity, specific small molecules targeting RNA. As part of...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb400265y
更新日期:2013-10-18 00:00:00
abstract::GPCRs mediate intracellular signaling upon external stimuli, making them ideal drug targets. However, little is known about their activation mechanisms due to the difficulty in purification. Here, we introduce a method to purify GPCRs in nanodiscs, which incorporates GPCRs into lipid bilayers immediately after membran...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb300466n
更新日期:2013-03-15 00:00:00
abstract::Chemical synthesis is a powerful method for precise modification of the structural and electronic properties of proteins. The difficulties in the synthesis and purification of peptides containing transmembrane segments have presented obstacles to the chemical synthesis of integral membrane proteins. Here, we present a...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb900210r
更新日期:2009-12-18 00:00:00
abstract::A two-way colorimetric biosensor based on unmodified gold nanoparticles (GNPs) and a switchable double-stranded DNA (dsDNA) concatemer have been demonstrated. Two hairpin probes (H1 and H2) were first designed that provided the fuels to assemble the dsDNA concatemers via hybridization chain reaction (HCR). A functiona...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.7b00060
更新日期:2017-05-19 00:00:00
abstract::Establishing the relative configuration of a bioactive natural product represents the most challenging part in determining its structure. Residual dipolar couplings (RDCs) are sensitive probes of the relative spatial orientation of internuclear vectors. We adapted a force field structure calculation methodology to all...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.7b00281
更新日期:2017-08-18 00:00:00
abstract::Vitamin A serves essential functions in mammalian biology as a signaling molecule and chromophore. This lipid can be synthesized from more than 50 putative dietary provitamin A precursor molecules which contain at least one unsubstituted β-ionone ring. We here scrutinized the enzymatic properties and substrate specifi...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.8b00290
更新日期:2018-08-17 00:00:00
abstract::Bacterial tRNA-guanine transglycosylase (Tgt) is involved in the biosynthesis of the modified tRNA nucleoside queuosine present in the anticodon wobble position of tRNAs specific for aspartate, asparagine, histidine, and tyrosine. Inactivation of the tgt gene leads to decreased pathogenicity of Shigella bacteria. Ther...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.0c00700
更新日期:2020-11-20 00:00:00
abstract::The Pygo-BCL9 complex is a chromatin reader, facilitating β-catenin-mediated oncogenesis, and is thus emerging as a potential therapeutic target for cancer. Its function relies on two ligand-binding surfaces of Pygo's PHD finger that anchor the histone H3 tail methylated at lysine 4 (H3K4me) with assistance from the B...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb500585s
更新日期:2014-12-19 00:00:00
abstract::Epigenetic regulation is directed, in part, by the correlated placement of histone post-translational modifications, but the mechanisms controlling correlated modifications are incompletely understood. Correlations arise from crosstalk among modifications and are frequently attributed to protein-protein interactions t...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb5004527
更新日期:2015-01-16 00:00:00
abstract::Sublancin 168 is a member of a small group of glycosylated antimicrobial peptides known as glycocins. The solution structure of sublancin 168, a 37-amino-acid peptide produced by Bacillus subtilis 168, has been solved by nuclear magnetic resonance (NMR) spectroscopy. Sublancin comprises two α-helices and a well-define...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb4008106
更新日期:2014-03-21 00:00:00
abstract::One-third of all human cancers harbor somatic RAS mutations. This leads to aberrant activation of downstream signaling pathways involving the RAF kinases. Current ATP-competitive RAF inhibitors are active in cancers with somatic RAF mutations, such as BRAF(V600) mutant melanomas. However, they paradoxically promote th...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb4003464
更新日期:2013-09-20 00:00:00
abstract::The receptor tyrosine kinase EPHA2 (Ephrin type-A receptor 2) plays important roles in oncogenesis, metastasis, and treatment resistance, yet therapeutic targeting, drug discovery, or investigation of EPHA2 biology is hampered by the lack of appropriate inhibitors and structural information. Here, we used chemical pro...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.6b00709
更新日期:2016-12-16 00:00:00
abstract::Flavin-containing monooxygenases (FMOs) are emerging as effective players in oxidative drug metabolism. Until recently, the functions of the five human FMO isoforms were mostly linked to their capability of oxygenating molecules containing soft N- and S-nucleophiles. However, the human FMO isoform 5 was recently shown...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.7b00470
更新日期:2017-09-15 00:00:00
abstract::In urodele amphibians, an early step in limb regeneration is skeletal muscle fiber dedifferentiation into a cellulate that proliferates to contribute new limb tissue. However, mammalian muscle cannot dedifferentiate after injury. We have developed a novel, small-molecule-based method to induce dedifferentiation in mam...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb200532v
更新日期:2012-04-20 00:00:00
abstract::Ubiquitin specific protease 7 (USP7) regulates the protein stability of key cellular regulators in pathways ranging from apoptosis to neuronal development, making it a promising therapeutic target. Here we used an engineered, activated variant of the USP7 catalytic domain to perform structure-activity studies of elect...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.0c00031
更新日期:2020-06-19 00:00:00
abstract::Cocaine esterase (CocE) is known as the most efficient natural enzyme for cocaine hydrolysis. The major obstacle to the clinical application of wild-type CocE is the thermoinstability with a half-life of only ∼12 min at 37 °C. The previously designed T172R/G173Q mutant (denoted as enzyme E172-173) with an improved in ...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb500257s
更新日期:2014-08-15 00:00:00
abstract::We have developed an improved tool for imaging acidic tumors by reporting the insertion of a transmembrane helix: the pHLIP-Fluorescence Insertion REporter (pHLIP-FIRE). In acidic tissues, such as tumors, peptides in the pHLIP family insert as α-helices across cell membranes. The cell-inserting end of the pHLIP-FIRE p...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb500388m
更新日期:2014-11-21 00:00:00
abstract::Mutations that constitutively activate the phosphatidyl-inositol-3-kinase (PI3K) signaling pathway, including alterations in PI3K, PTEN, and AKT, are found in a variety of human cancers, implicating the PI3K lipid kinase as an attractive target for the development of therapeutic agents to treat cancer and other relate...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb800039y
更新日期:2008-05-16 00:00:00
abstract::Ca(II) ions are critical for the proper function of neurons by contributing to synaptic signaling and regulating neuronal plasticity. Dysregulation of Ca(II) is associated with a number of pathologies that cause neurodegeneration; therefore the ability to monitor Ca(II) intracellularly is an important target for molec...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.9b00638
更新日期:2020-02-21 00:00:00
abstract::Inducible modulation is often required for precise investigations and manipulations of dynamic biological processes. Transcription activator-like effectors (TALEs) provide a powerful tool for targeted gene editing and transcriptional programming. We designed a series of chemical inducible systems by coupling TALEs wit...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.7b00606
更新日期:2018-03-16 00:00:00
abstract::STAT family proteins are important mediators of cell signaling and represent therapeutic targets for the treatment of human diseases. Most STAT inhibitors target the protein-protein interaction domain, the SH2 domain, but specificity for a single STAT protein is often limited. Recently, we developed catechol bisphosph...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.9b00137
更新日期:2019-04-19 00:00:00
abstract::The expansion of the genetic code with noncanonical amino acids (ncAA) enables the function of proteins to be tailored with high molecular precision. In this approach, the ncAA is charged to an orthogonal nonsense suppressor tRNA by an aminoacyl-tRNA-synthetase (aaRS) and incorporated into the target protein in vivo b...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb5006273
更新日期:2014-12-19 00:00:00
abstract::RAGE (Receptor for Advanced Glycation End-Products) has emerged as a major receptor that mediates vascular inflammation. Signaling through RAGE by damage-associated molecular pattern molecules often leads to uncontrolled inflammation that exacerbates the impact of the underlying disease. Oligomerization of RAGE is bel...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb4001553
更新日期:2013-07-19 00:00:00
abstract::How primitive enzymes emerged from a primordial pool remains a fundamental unanswered question with important practical implications in synthetic biology. Here we show that a de novo evolved ATP binding protein, selected solely on the basis of its ability to bind ATP, mediates the regiospecific hydrolysis of ATP to AD...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb900109w
更新日期:2009-08-21 00:00:00
abstract::Reactive oxygen species (ROS) play an important role in the onset of Parkinson's disease (PD), and deciphering protective mechanisms is a major goal for therapeutic development. Here, DJ-1 (PARK7) gained major attention when a conserved cysteine residue with a putative role in oxidative stress sensing/protection was l...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/acschembio.8b00633
更新日期:2018-08-17 00:00:00
abstract::The Morita-Baylis-Hillman reaction forms a carbon-carbon bond between the α-carbon of a conjugated carbonyl compound and a carbon electrophile. The reaction mechanism involves Michael addition of a nucleophile catalyst at the carbonyl β-carbon, followed by bond formation with the electrophile and catalyst disassociati...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb3006227
更新日期:2013-04-19 00:00:00
abstract::The genetic code specifies 20 common amino acids and is largely preserved in both single and multicellular organisms. Unnatural amino acids (Uaas) have been genetically incorporated into proteins by using engineered orthogonal tRNA/aminoacyl-tRNA synthetase (RS) pairs, enabling new research capabilities and precision ...
journal_title:ACS chemical biology
pub_type: 杂志文章
doi:10.1021/cb200542j
更新日期:2012-07-20 00:00:00