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Sensing proteins through nanopores: fundamental to applications.

Abstract：

:Proteins subjected to an electric field and forced to pass through a nanopore induce blockades of ionic current that depend on the protein and nanopore characteristics and interactions between them. Recent advances in the analysis of these blockades have highlighted a variety of phenomena that can be used to study protein translocation and protein folding, to probe single-molecule catalytic reactions in order to obtain kinetic and thermodynamic information, and to detect protein-antibody complexes, proteins with DNA and RNA aptamers, and protein-pore interactions. Nanopore design is now well controlled, allowing the development of future biotechnologies and medicine applications.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Oukhaled A,Bacri L,Pastoriza-Gallego M,Betton JM,Pelta J

doi

10.1021/cb300449t

subject

Has Abstract

pub_date

2012-12-21 00:00:00

pages

1935-49

issue

12

eissn

1554-8929

issn

1554-8937

journal_volume

7

pub_type

杂志文章,评审

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