Abstract：
:The display and analysis of proteins expressed on biological surfaces has become an attractive tool for the study of molecular interactions in enzymology, protein engineering, and high-throughput screening. Among the growing number of established display systems, retroviruses offer a unique and fully mammalian platform for the expression of correctly folded and post-translationally modified proteins in the context of cell plasma membrane-derived particles. This is of special interest for therapeutic applications such as gene therapy and vaccine development and also offers advantages for the engineering of mammalian proteins toward customized binding affinities and catalytic activities. This review critically summarizes the basic concepts and applications of retroviral display and analyses its benefits in comparison to other display techniques.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Urban JH,Merten CAdoi
10.1021/cb100285nsubject
Has Abstractpub_date
2011-01-21 00:00:00pages
61-74issue
1eissn
1554-8929issn
1554-8937journal_volume
6pub_type
杂志文章,评审abstract：:Creation of an artificial oscillating gene expression system is one of the most challenging issues in synthetic biology. Here, we constructed a simple system to manipulate gene expression patterns to be circadian, reflecting the intrinsic cellular clock, by fusing a core clock protein, BMAL1 or CLOCK, with a zinc fing...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300432s
更新日期：2012-11-16 00:00:00
abstract：:Complexity generation in naturally occurring peptide scaffolds can occur either by posttranslational modifications of nascent ribosomal proteins or through post assembly line tailoring of nonribosomal peptides. Short enzymatic pathways utilizing bimodular and trimodular nonribosomal peptide synthetase (NRPS) assembly ...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb4001684
更新日期：2013-07-19 00:00:00
abstract：:Although protein kinase inhibitors present excellent pharmaceutical opportunities, lack of selectivity and associated therapeutic side effects are common. Bisubstrate-based inhibitors targeting both the high-selectivity peptide substrate binding groove and the high-affinity ATP pocket address this. However, they are t...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300709g
更新日期：2013-07-19 00:00:00
abstract：:Endocytosis is a key process in cellular delivery of macromolecules by molecular transporters, although the mechanism of internalization remains unclear. Here, we probe the cellular uptake of streptavidin using biotinylated guanidinoneomycin (biotinGNeo), a low molecular weight guanidinium-rich molecular transporter. ...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/cb400172h
更新日期：2013-07-19 00:00:00
abstract：:RAGE (Receptor for Advanced Glycation End-Products) has emerged as a major receptor that mediates vascular inflammation. Signaling through RAGE by damage-associated molecular pattern molecules often leads to uncontrolled inflammation that exacerbates the impact of the underlying disease. Oligomerization of RAGE is bel...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb4001553
更新日期：2013-07-19 00:00:00
abstract：:Despite the stereospecificity of translation for l-amino acids (l-AAs) in vivo, synthetic biologists have enabled ribosomal incorporation of d-AAs in vitro toward encoding polypeptides with pharmacologically desirable properties. However, the steps in translation limiting d-AA incorporation need clarification. In this...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00952
更新日期：2019-02-15 00:00:00
abstract：:Epitranscriptomic modifications play an important role in RNA function and can impact gene expression. Here, we apply a chemical proteomics approach to investigate readers of N1-methyladenosine (m1A), a poorly characterized modification on mammalian mRNA. We find that YTHDF proteins, known m6A readers, recognize m1A-m...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00655
更新日期：2020-01-17 00:00:00
abstract：:Detection of metabolites and post-translational modifications can be achieved using the azide as a bioorthogonal chemical reporter. Once introduced into target biomolecules, either metabolically or through chemical modification, the azide can be tagged with probes using one of three highly selective reactions: the Sta...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/cb6003228
更新日期：2006-11-21 00:00:00
abstract：:Combinatorial libraries built with severely restricted chemical diversity have yielded highly functional synthetic binding proteins. Structural analyses of these minimalist binding sites have revealed the dominant role of large tyrosine residues for mediating molecular contacts and of small serine/glycine residues for...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb800314v
更新日期：2009-05-15 00:00:00
abstract：:Vitamin A serves essential functions in mammalian biology as a signaling molecule and chromophore. This lipid can be synthesized from more than 50 putative dietary provitamin A precursor molecules which contain at least one unsubstituted β-ionone ring. We here scrutinized the enzymatic properties and substrate specifi...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00290
更新日期：2018-08-17 00:00:00
abstract：:The hypoxia inducible factor (HIF) system is central to the signaling of low oxygen (hypoxia) in animals. The levels of HIF-α isoforms are regulated in an oxygen-dependent manner by the activity of the HIF prolyl-hydroxylases (PHD or EGLN enzymes), which are Fe(II) and 2-oxoglutarate (2OG) dependent oxygenases. Here, ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400088q
更新日期：2013-07-19 00:00:00
abstract：:BRD4, a member of the bromodomain and extraterminal domain (BET) family, has emerged as a promising epigenetic target in cancer and inflammatory disorders. All reported BET family ligands bind within the bromodomain acetyl-lysine binding sites and competitively inhibit BET protein interaction with acetylated chromatin...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00058
更新日期：2020-04-17 00:00:00
abstract：:Enzymatic plasticity, as a modern term referring to the functional conversion of an enzyme, is significant for enzymatic activity redesign. The bacterial diterpene cyclase CotB2 is a typical plastic enzyme by which its native form precisely conducts a chemical reaction while its mutants diversify the catalytic functio...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00645
更新日期：2020-10-16 00:00:00
abstract：:ABCG2 is a membrane-localized, human transporter protein that has been demonstrated to reduce the intracellular accumulation of substrates through ATP-dependent efflux. Highly expressed in placental syncytiotrophoblasts, brain microvasculature, and the gastrointestinal tract, ABCG2 has been shown to mediate normal tis...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb900134c
更新日期：2009-08-21 00:00:00
abstract：:Binding of the Fc domain of Immunoglobulin G (IgG) to Fcγ receptors on leukocytes can initiate a series of signaling events resulting in antibody-dependent cell-mediated cytotoxicity (ADCC) and other important immune responses. Fc domains lacking glycosylation at N297 have greatly diminished Fcγ receptor binding and l...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300130k
更新日期：2012-09-21 00:00:00
abstract：:Available crystal structures of opioid receptors provide a high-resolution picture of ligand binding at the primary ("orthosteric") site, that is, the site targeted by endogenous ligands. Recently, positive allosteric modulators of opioid receptors have also been discovered, but their modes of binding and action remai...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00712
更新日期：2016-05-20 00:00:00
abstract：:The ubiquity of microorganisms is unparalleled in any other known organism. These creatures surround our outsides and colonize our insides, a fact that has been known for centuries. However, despite their prevalence and long study, many of their characteristics still remain largely unexplained, including how proteins ...
journal_title：ACS chemical biology
pub_type： 传,历史文章,杂志文章
doi：10.1021/cb100179t
更新日期：2010-07-16 00:00:00
abstract：:Cutaneous leishmaniasis remains ignored in therapeutic drug discovery programs worldwide. This is mainly because cutaneous leishmaniasis is frequently a disease of impoverished populations in countries where funds are limited for research and patient care. However, the health burden of individuals in endemic areas man...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400800q
更新日期：2014-03-21 00:00:00
abstract：:Alzheimer's Disease (AD) is a progressive neurodegenerative disease and the most common cause of dementia. The current treatment options for AD are limited to ameliorating cognitive decline temporarily and not reversing or preventing the progression of dementia. Hence, more effective therapeutic strategies are needed ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00851
更新日期：2020-12-18 00:00:00
abstract：:The quest for ever more selective kinase inhibitors as potential future drugs has yielded a large repertoire of chemical probes that are selective for specific kinase conformations. These probes have been useful tools to obtain structural snapshots of kinase conformational plasticity. Similarly, kinetic and thermodyna...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb500870a
更新日期：2015-01-16 00:00:00
abstract：:Whereas the C. elegans genome was sequenced many years ago, the role of small molecule signals in its biology is still poorly understood. A recent publication reports the identification of two steroidal signaling molecules that regulate C. elegans reproductive development and dauer diapause via the nuclear receptor DA...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb600173t
更新日期：2006-05-23 00:00:00
abstract：:Toxic heavy metals have been considered to be harmful environmental contaminations. The molecular mechanisms of heavy-metals-induced cytotoxicity and carcinogenicity are still not well elucidated. Previous reports showed exposures to toxic heavy metals can cause a change of DNA cytosine methylation (5-methylcytosine, ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00170
更新日期：2017-06-16 00:00:00
abstract：:Photo- or optoacoustics (OA) imaging is increasingly being used as a non-invasive imaging method that can simultaneously reveal structure and function in deep tissue. However, the most frequent transgenic OA labels are current fluorescent proteins that are not optimized for OA imaging. Thus, they lack OA signal streng...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00299
更新日期：2019-09-20 00:00:00
abstract：:Diversity of scaffold structure and function is a hallmark of the >50,000 isoprenoid natural products such as taxol. Whereas most members of this class are assembled by iterative head-to-tail enzymatic joining reactions between delta2- and delta3-isopentenyl diphosphate (IPP) monomers, dimerization of two delta2-IPP m...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb700094s
更新日期：2007-05-22 00:00:00
abstract：:We recently reported two novel tools for precisely controlling and quantifying Cas9 activity: a chemically inducible Cas9 variant (ciCas9) that can be rapidly activated by small molecules and a ddPCR assay for time-resolved measurement of DNA double strand breaks (DSB-ddPCR). Here, we further demonstrate the potential...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00652
更新日期：2018-02-16 00:00:00
abstract：:Polyalkoxybenzenes are plant components displaying a wide range of biological activities. In these studies, we synthesized apiol and dillapiol isoxazoline analogues of combretastatins and evaluated their effect on sea urchin embryos. We have shown that p-methoxyphenyl isoxazoline caused sea urchin embryo immobilizatio...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/cb700163q
更新日期：2008-02-15 00:00:00
abstract：:Large-scale quantification of protein O-linked β- N-acetylglucosamine (O-GlcNAc) modification in a site-specific manner remains a key challenge in studying O-GlcNAc biology. Herein, we developed an isotope-tagged cleavable linker (isoTCL) strategy, which enabled isotopic labeling of O-GlcNAc through bioorthogonal conj...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00414
更新日期：2018-08-17 00:00:00
abstract：:Peptidoglycan glycosyltransferases (PGTs), enzymes that catalyze the formation of the glycan chains of the bacterial cell wall, have tremendous potential as antibiotic targets. The moenomycins, a potent family of natural product antibiotics, are the only known active site inhibitors of the PGTs and serve as blueprints...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb800078a
更新日期：2008-07-18 00:00:00
abstract：:CRISPR-Cas genome editing technologies have revolutionized modern molecular biology by making targeted DNA edits simple and scalable. These technologies are developed by domesticating naturally occurring microbial adaptive immune systems that display wide diversity of functionality for targeted nucleic acid cleavage. ...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/acschembio.7b00800
更新日期：2018-02-16 00:00:00
abstract：:Autophagy is a bulk, nonspecific protein degradation pathway that is involved in the pathogenesis of cancer and neurodegenerative disease. Here, we observed that xanthohumol (XN), a prenylated chalcone present in hops (Humulus lupulus L.) and beer, modulates autophagy. By using XN-immobilized beads, valosin-containing...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb200492h
更新日期：2012-05-18 00:00:00
