Abstract:
:Here we describe a protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient organ clearing, rapid imaging by light-sheet microscopy and quantitative imaging analysis of multiple samples. The organ or body is cleared by immersion for 1-14 d, with the exact time required dependent on the sample type and the experimental purposes. A single imaging set can be completed in 30-60 min. Image processing and analysis can take <1 d, but it is dependent on the number of samples in the data set. The CUBIC clearing protocol can process multiple samples simultaneously. We previously used CUBIC to image whole-brain neural activities at single-cell resolution using Arc-dVenus transgenic (Tg) mice. CUBIC informatics calculated the Venus signal subtraction, comparing different brains at a whole-organ scale. These protocols provide a platform for organism-level systems biology by comprehensively detecting cells in a whole organ or body.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Susaki EA,Tainaka K,Perrin D,Yukinaga H,Kuno A,Ueda HRdoi
10.1038/nprot.2015.085subject
Has Abstractpub_date
2015-11-01 00:00:00pages
1709-27issue
11eissn
1754-2189issn
1750-2799pii
nprot.2015.085journal_volume
10pub_type
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