Abstract:
:Carbohydrate microarrays have received considerable attention as an advanced technology for the rapid analysis of carbohydrate-protein interactions. This protocol provides detailed procedures for the preparation of carbohydrate microarrays by immobilizing hydrazide-conjugated carbohydrates on epoxide-derivatized glass slides. In addition, we describe the use we make of these microarrays in glycomics research. Unlike other techniques that require large amounts of samples and long assay times, carbohydrate microarrays are used to carry out the rapid assessment of a number of carbohydrate-recognition events with tiny amounts of carbohydrate samples. Furthermore, the microarray technology is also utilized for the rapid assay of enzyme activities. We are able to routinely prepare carbohydrate microarrays within 12 h by using hydrazide-conjugated carbohydrates and apply these microarrays for the studies of glycan-protein interactions within 8 h.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Park S,Lee MR,Shin Idoi
10.1038/nprot.2007.373subject
Has Abstractpub_date
2007-01-01 00:00:00pages
2747-58issue
11eissn
1754-2189issn
1750-2799pii
nprot.2007.373journal_volume
2pub_type
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