Abstract:
:The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Herbert BS,Hochreiter AE,Wright WE,Shay JWdoi
10.1038/nprot.2006.239subject
Has Abstractpub_date
2006-01-01 00:00:00pages
1583-90issue
3eissn
1754-2189issn
1750-2799pii
nprot.2006.239journal_volume
1pub_type
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